Restriction map for
pFN21ASDA0139
(Linear) (Six-base) MAPSORT of: KIAA0139.seq Check: 3856 from: 1 to: 4146
KIAA0139.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1314 4146
Size: 1314 2832
AccI GT'mk_AC
Cuts at: 0 955 4146
Size: 955 3191
AclI AA'CG_TT
Cuts at: 0 2106 4146
Size: 2106 2040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 1529 2729 3699 4146
Size: 323 1206 1200 970 447
Fragments arranged by size:
1206 1200 970 447 323
AfeI AGC'GCT
Cuts at: 0 2136 4146
Size: 2136 2010
AflIII A'CryG_T
Cuts at: 0 565 4146
Size: 565 3581
AhdI GACnn_n'nnGTC
Cuts at: 0 3514 4146
Size: 3514 632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2911 2943 4146
Size: 2911 32 1203
Fragments arranged by size:
2911 1203 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2719 4146
Size: 2719 1427
ApaI G_GGCC'C
Cuts at: 0 3415 4146
Size: 3415 731
ApoI r'AATT_y
Cuts at: 0 49 451 547 577 1189 1677 2117 3380
Size: 49 402 96 30 612 488 440 1263
Cuts at: 3380 4146
Size: 766
Fragments arranged by size:
1263 766 612 488 440 402 96 49
30
AseI AT'TA_AT
Cuts at: 0 1112 4146
Size: 1112 3034
AvaI C'yCGr_G
Cuts at: 0 1502 3575 4146
Size: 1502 2073 571
Fragments arranged by size:
2073 1502 571
AvrII C'CTAG_G
Cuts at: 0 3724 4146
Size: 3724 422
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2512 2545 4146
Size: 2512 33 1601
Fragments arranged by size:
2512 1601 33
BamHI G'GATC_C
Cuts at: 0 1700 1992 4146
Size: 1700 292 2154
Fragments arranged by size:
2154 1700 292
BanI G'GyrC_C
Cuts at: 0 1945 2689 4146
Size: 1945 744 1457
Fragments arranged by size:
1945 1457 744
BanII G_rGCy'C
Cuts at: 0 2843 3415 4146
Size: 2843 572 731
Fragments arranged by size:
2843 731 572
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 1072 1982 2270 2634 3017 3726 4146
Size: 410 662 910 288 364 383 709 420
Fragments arranged by size:
910 709 662 420 410 383 364 288
BbvCI CC'TCA_GC
Cuts at: 0 1820 3758 4146
Size: 1820 1938 388
Fragments arranged by size:
1938 1820 388
BclI T'GATC_A
Cuts at: 0 1452 4146
Size: 1452 2694
BfrBI ATG'CAT
Cuts at: 0 684 877 4146
Size: 684 193 3269
Fragments arranged by size:
3269 684 193
BglI GCCn_nnn'nGGC
Cuts at: 0 2964 4146
Size: 2964 1182
BglII A'GATC_T
Cuts at: 0 3861 4146
Size: 3861 285
Bme1580I G_kGCm'C
Cuts at: 0 3415 4146
Size: 3415 731
BmgBI CAC'GTC
Cuts at: 0 2758 2908 3118 3451 3934 4146
Size: 2758 150 210 333 483 212
Fragments arranged by size:
2758 483 333 212 210 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 1951 4146
Size: 1951 2195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4077 4146
Size: 4077 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1786 2050 2715 3762 3795 4146
Size: 277 1509 264 665 1047 33 351
Fragments arranged by size:
1509 1047 665 351 277 264 33
Bpu10I CC'TnA_GC
Cuts at: 0 998 1820 3758 4146
Size: 998 822 1938 388
Fragments arranged by size:
1938 998 822 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1765 2002 3474 3768 4146
Size: 76 1689 237 1472 294 378
Fragments arranged by size:
1689 1472 378 294 237 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 2789 3501 4004 4016 4022 4146
Size: 2789 712 503 12 6 124
Fragments arranged by size:
2789 712 503 124 12 6
BsaBI GATnn'nnATC
Cuts at: 0 3224 3344 3434 3461 3698 4146
Size: 3224 120 90 27 237 448
Fragments arranged by size:
3224 448 237 120 90 27
BsaHI Gr'CG_yC
Cuts at: 0 2814 3027 4146
Size: 2814 213 1119
Fragments arranged by size:
2814 1119 213
BsaWI w'CCGG_w
Cuts at: 0 1263 4146
Size: 1263 2883
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1741 2449 2452 3164 3571 3664 3968 4146
Size: 1741 708 3 712 407 93 304 178
Fragments arranged by size:
1741 712 708 407 304 178 93 3
BseYI C'CCAG_C
Cuts at: 0 533 3563 3996 4146
Size: 533 3030 433 150
Fragments arranged by size:
3030 533 433 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2282 3416 4146
Size: 2282 1134 730
Fragments arranged by size:
2282 1134 730
BsiHKAI G_wGCw'C
Cuts at: 0 1694 4146
Size: 1694 2452
BsiWI C'GTAC_G
Cuts at: 0 1003 4146
Size: 1003 3143
BsmI GAATG_Cn'
Cuts at: 0 611 745 1954 2261 4146
Size: 611 134 1209 307 1885
Fragments arranged by size:
1885 1209 611 307 134
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2748 3863 3924 4146
Size: 2748 1115 61 222
Fragments arranged by size:
2748 1115 222 61
Bsp1286I G_dGCh'C
Cuts at: 0 1694 2843 3415 4146
Size: 1694 1149 572 731
Fragments arranged by size:
1694 1149 731 572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1314 1431 4146
Size: 1314 117 2715
Fragments arranged by size:
2715 1314 117
BsrBI CCG'CTC
Cuts at: 0 2475 2538 2805 3969 4146
Size: 2475 63 267 1164 177
Fragments arranged by size:
2475 1164 267 177 63
BsrDI GCAATG_nn'
Cuts at: 0 1428 4146
Size: 1428 2718
BsrFI r'CCGG_y
Cuts at: 0 3 2954 3836 4146
Size: 3 2951 882 310
Fragments arranged by size:
2951 882 310 3
BssHII G'CGCG_C
Cuts at: 0 2490 4146
Size: 2490 1656
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1281 1539 4146
Size: 1281 258 2607
Fragments arranged by size:
2607 1281 258
BstXI CCAn_nnnn'nTGG
Cuts at: 0 687 2109 3530 4146
Size: 687 1422 1421 616
Fragments arranged by size:
1422 1421 687 616
BstYI r'GATC_y
Cuts at: 0 177 348 1480 1700 1992 2166 3861 4146
Size: 177 171 1132 220 292 174 1695 285
Fragments arranged by size:
1695 1132 292 285 220 177 174 171
Bsu36I CC'TnA_GG
Cuts at: 0 2915 4146
Size: 2915 1231
BtgI C'CryG_G
Cuts at: 0 442 4146
Size: 442 3704
BtsI GCAGTG_nn'
Cuts at: 0 481 1661 4146
Size: 481 1180 2485
Fragments arranged by size:
2485 1180 481
ClaI AT'CG_AT
Cuts at: 0 2367 4146
Size: 2367 1779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3507 3542 4146
Size: 3507 35 604
Fragments arranged by size:
3507 604 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1575 4146
Size: 1575 2571
EarI CTCTTCn'nnn_
Cuts at: 0 1820 2333 2426 2474 2510 2615 2724 2787
Size: 1820 513 93 48 36 105 109 63
Cuts at: 2787 2795 3741 3879 3959 4146
Size: 8 946 138 80 187
Fragments arranged by size:
1820 946 513 187 138 109 105 93
80 63 48 36 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1300 4146
Size: 1300 2846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 323 1529 1786 2050 2715 2729 3699
Size: 277 46 1206 257 264 665 14 970
Cuts at: 3699 3762 3795 4146
Size: 63 33 351
Fragments arranged by size:
1206 970 665 351 277 264 257 63
46 33 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3056 4146
Size: 3056 1090
EcoO109I rG'GnC_Cy
Cuts at: 0 2704 2899 3202 3232 3262 3292 3322 3352
Size: 2704 195 303 30 30 30 30 30
Cuts at: 3352 3411 3412 3663 3889 3972 4146
Size: 59 1 251 226 83 174
Fragments arranged by size:
2704 303 251 226 195 174 83 59
30 30 30 30 30 1
EcoRI G'AATT_C
Cuts at: 0 577 3380 4146
Size: 577 2803 766
Fragments arranged by size:
2803 766 577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 210 1559 1759 4146
Size: 210 1349 200 2387
Fragments arranged by size:
2387 1349 210 200
HaeII r_GCGC'y
Cuts at: 0 516 630 1743 2138 3158 4146
Size: 516 114 1113 395 1020 988
Fragments arranged by size:
1113 1020 988 516 395 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 661 693 909 941 1509 1541 1692 1724
Size: 661 32 216 32 568 32 151 32
Cuts at: 1724 2767 2799 2914 2946 3154 3157 3186 3189
Size: 1043 32 115 32 208 3 29 3
Cuts at: 3189 3500 3532 3552 3584 3881 3913 4072 4104
Size: 311 32 20 32 297 32 159 32
Cuts at: 4104 4146
Size: 42
Fragments arranged by size:
1043 661 568 311 297 216 208 159
151 115 42 32 32 32 32 32
32 32 32 32 32 29 20 3
3
HincII GTy'rAC
Cuts at: 0 235 4146
Size: 235 3911
HindIII A'AGCT_T
Cuts at: 0 539 4146
Size: 539 3607
MfeI C'AATT_G
Cuts at: 0 1173 1721 4146
Size: 1173 548 2425
Fragments arranged by size:
2425 1173 548
MluI A'CGCG_T
Cuts at: 0 565 4146
Size: 565 3581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1419 2567 3197 3317 4146
Size: 1419 1148 630 120 829
Fragments arranged by size:
1419 1148 829 630 120
MslI CAynn'nnrTG
Cuts at: 0 3035 4146
Size: 3035 1111
MspA1I CmG'CkG
Cuts at: 0 708 1566 1698 3094 3757 4146
Size: 708 858 132 1396 663 389
Fragments arranged by size:
1396 858 708 663 389 132
NaeI GCC'GGC
Cuts at: 0 5 4146
Size: 5 4141
NcoI C'CATG_G
Cuts at: 0 442 4146
Size: 442 3704
NgoMIV G'CCGG_C
Cuts at: 0 3 4146
Size: 3 4143
NsiI A_TGCA'T
Cuts at: 0 686 879 4146
Size: 686 193 3267
Fragments arranged by size:
3267 686 193
NspI r_CATG'y
Cuts at: 0 1542 4146
Size: 1542 2604
PflMI CCAn_nnn'nTGG
Cuts at: 0 3118 4146
Size: 3118 1028
PpuMI rG'GwC_Cy
Cuts at: 0 2704 3202 3232 3262 3292 3322 3352 3663
Size: 2704 498 30 30 30 30 30 311
Cuts at: 3663 3889 3972 4146
Size: 226 83 174
Fragments arranged by size:
2704 498 311 226 174 83 30 30
30 30 30
PshAI GACnn'nnGTC
Cuts at: 0 3200 3260 3290 3320 3785 4146
Size: 3200 60 30 30 465 361
Fragments arranged by size:
3200 465 361 60 30 30
PspOMI G'GGCC_C
Cuts at: 0 3411 4146
Size: 3411 735
PstI C_TGCA'G
Cuts at: 0 1322 4146
Size: 1322 2824
PvuII CAG'CTG
Cuts at: 0 708 1566 3094 3757 4146
Size: 708 858 1528 663 389
Fragments arranged by size:
1528 858 708 663 389
SanDI GG'GwC_CC
Cuts at: 0 3202 3232 3262 3292 3322 3972 4146
Size: 3202 30 30 30 30 650 174
Fragments arranged by size:
3202 650 174 30 30 30 30
SapI GCTCTTCn'nnn_
Cuts at: 0 2333 2510 2795 3959 4146
Size: 2333 177 285 1164 187
Fragments arranged by size:
2333 1164 285 187 177
ScaI AGT'ACT
Cuts at: 0 1586 4146
Size: 1586 2560
SexAI A'CCwGG_T
Cuts at: 0 3495 4146
Size: 3495 651
SfcI C'TryA_G
Cuts at: 0 1318 2418 4146
Size: 1318 1100 1728
Fragments arranged by size:
1728 1318 1100
SmlI C'TyrA_G
Cuts at: 0 55 1502 1744 1981 3489 3575 3783 4146
Size: 55 1447 242 237 1508 86 208 363
Fragments arranged by size:
1508 1447 363 242 237 208 86 55
SphI G_CATG'C
Cuts at: 0 1542 4146
Size: 1542 2604
SspI AAT'ATT
Cuts at: 0 360 1758 4146
Size: 360 1398 2388
Fragments arranged by size:
2388 1398 360
StuI AGG'CCT
Cuts at: 0 2988 4060 4146
Size: 2988 1072 86
Fragments arranged by size:
2988 1072 86
StyI C'CwwG_G
Cuts at: 0 442 3355 3415 3502 3724 4146
Size: 442 2913 60 87 222 422
Fragments arranged by size:
2913 442 422 222 87 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1933 3896 4028 4040 4046 4146
Size: 1933 1963 132 12 6 100
Fragments arranged by size:
1963 1933 132 100 12 6
TatI w'GTAC_w
Cuts at: 0 1134 1584 4146
Size: 1134 450 2562
Fragments arranged by size:
2562 1134 450
Tth111I GACn'n_nGTC
Cuts at: 0 2909 3497 4146
Size: 2909 588 649
Fragments arranged by size:
2909 649 588
XbaI T'CTAG_A
Cuts at: 0 351 502 907 2650 4146
Size: 351 151 405 1743 1496
Fragments arranged by size:
1743 1496 405 351 151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2747 3213 3243 3273 3303 3333 4146
Size: 2747 466 30 30 30 30 813
Fragments arranged by size:
2747 813 466 30 30 30 30
XhoI C'TCGA_G
Cuts at: 0 1502 3575 4146
Size: 1502 2073 571
Fragments arranged by size:
2073 1502 571
XmnI GAAnn'nnTTC
Cuts at: 0 498 764 2117 4146
Size: 498 266 1353 2029
Fragments arranged by size:
2029 1353 498 266
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AhdI AloI
AlwNI ApaI ApoI AseI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BclI BfrBI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI
DrdI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII MfeI MluI MmeI MslI
MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI PpuMI
PshAI PspOMI PstI PvuII SanDI SapI ScaI SexAI
SfcI SmlI SphI SspI StuI StyI TaqII TatI
Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI AleI AlfI ApaLI AscI
AsiSI BbeI BcgI BciVI BlpI BmtI BsaAI BsaXI
BsiEI BspEI BspHI BsrGI BssSI BstBI BstEII BstZ17I
BtgZI DraI DraIII EaeI EagI EcoICRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MscI NarI NdeI
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI TstI XmaI ZraI