(Circular) (Six-base) MAPSORT of: pFN21ASDA0132.seq Check: 3728 from: 1 to: 6601 pFN21ASDA0132 6601 bp With 182 enzymes: SgfI * April 10, 2017 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2956 3088 2956 Size: 132 6469 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2259 4411 278 Size: 53 83 186 936 723 2152 2468 Fragments arranged by size: 2468 2152 936 723 186 83 53 AccI GT'mk_AC Cuts at: 1937 3944 1937 Size: 2007 4594 Acc65I G'GTAC_C Cuts at: 3922 3922 Size: 6601 AclI AA'CG_TT Cuts at: 1563 4728 5101 1563 Size: 3165 373 3063 Fragments arranged by size: 3165 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2161 4664 6048 6316 1632 Size: 529 2503 1384 268 1917 Fragments arranged by size: 2503 1917 1384 529 268 AfeI AGC'GCT Cuts at: 3629 3629 Size: 6601 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6404 AflIII A'CryG_T Cuts at: 1203 3747 5500 6477 1203 Size: 2544 1753 977 1327 Fragments arranged by size: 2544 1753 1327 977 AgeI A'CCGG_T Cuts at: 5432 5432 Size: 6601 AhdI GACnn_n'nnGTC Cuts at: 5330 5330 Size: 6601 AleI CACnn'nnGTG Cuts at: 1166 2625 2966 3336 1166 Size: 1459 341 370 4431 Fragments arranged by size: 4431 1459 370 341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2643 3574 1184 Size: 1459 931 4211 Fragments arranged by size: 4211 1459 931 AlwNI CAG_nnn'CTG Cuts at: 2248 2557 2821 4344 5916 2248 Size: 309 264 1523 1572 2933 Fragments arranged by size: 2933 1572 1523 309 264 ApaLI G'TGCA_C Cuts at: 4658 5814 4658 Size: 1156 5445 ApoI r'AATT_y Cuts at: 1493 2435 3910 4136 4288 6397 1493 Size: 942 1475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 942 226 152 AseI AT'TA_AT Cuts at: 160 1032 5155 160 Size: 872 4123 1606 Fragments arranged by size: 4123 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 AvaI C'yCGr_G Cuts at: 1870 1943 2631 3926 1870 Size: 73 688 1295 4545 Fragments arranged by size: 4545 1295 688 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3559 3592 3559 Size: 33 6568 BamHI G'GATC_C Cuts at: 3931 4298 3931 Size: 367 6234 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2983 3071 3100 3922 5377 Size: 325 217 627 1196 88 29 822 1455 Cuts at: 5377 618 Size: 1842 Fragments arranged by size: 1842 1455 1196 822 627 325 217 88 29 BanII G_rGCy'C Cuts at: 1391 2946 3858 3920 1391 Size: 1555 912 62 4072 Fragments arranged by size: 4072 1555 912 62 BbeI G_GCGC'C Cuts at: 2987 2987 Size: 6601 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2332 2749 928 Size: 971 433 417 4780 Fragments arranged by size: 4780 971 433 417 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2557 2591 2845 2879 4792 4826 1903 Size: 34 620 34 254 34 1913 34 3678 Fragments arranged by size: 3678 1913 620 254 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2414 4493 5703 2414 Size: 2079 1210 3312 Fragments arranged by size: 3312 2079 1210 BclI T'GATC_A Cuts at: 1548 3519 1548 Size: 1971 4630 BfrBI ATG'CAT Cuts at: 2650 2650 Size: 6601 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2447 3120 5212 Size: 107 122 71 631 605 775 673 2092 Cuts at: 5212 136 Size: 1525 Fragments arranged by size: 2092 1525 775 673 631 605 122 107 71 BglII A'GATC_T Cuts at: 2931 6596 2931 Size: 3665 2936 BlpI GC'TnA_GC Cuts at: 2237 2595 3038 4018 2237 Size: 358 443 980 4820 Fragments arranged by size: 4820 980 443 358 Bme1580I G_kGCm'C Cuts at: 1790 2093 2632 3258 3691 4662 5818 1790 Size: 303 539 626 433 971 1156 2573 Fragments arranged by size: 2573 1156 971 626 539 433 303 BmgBI CAC'GTC Cuts at: 1318 2485 3651 1318 Size: 1167 1166 4268 Fragments arranged by size: 4268 1167 1166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2290 2785 3057 Size: 416 53 83 399 24 873 495 272 Cuts at: 3057 4332 5290 442 Size: 1275 958 1753 Fragments arranged by size: 1753 1275 958 873 495 416 399 272 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 3068 3175 3814 5261 1239 Size: 971 858 107 639 1447 2579 Fragments arranged by size: 2579 1447 971 858 639 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4726 5591 5889 4726 Size: 865 298 5438 Fragments arranged by size: 5438 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5264 882 Size: 617 3765 2219 Fragments arranged by size: 3765 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3898 4392 493 Size: 711 2694 494 2702 Fragments arranged by size: 2702 2694 711 494 BsaBI GATnn'nnATC Cuts at: 6595 6595 Size: 6601 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2150 2256 2852 2984 Size: 53 83 186 936 617 106 596 132 Cuts at: 2984 3555 4408 4790 5425 275 Size: 571 853 382 635 1451 Fragments arranged by size: 1451 936 853 635 617 596 571 382 186 132 106 83 53 BsaWI w'CCGG_w Cuts at: 3081 5034 5432 5706 5853 6264 3081 Size: 1953 398 274 147 411 3418 Fragments arranged by size: 3418 1953 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2375 2405 2435 2465 3173 Size: 30 165 30 972 30 30 30 708 Cuts at: 3173 3203 1178 Size: 30 4576 Fragments arranged by size: 4576 972 708 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 2954 1186 Size: 1504 264 4833 Fragments arranged by size: 4833 1504 264 BseYI C'CCAG_C Cuts at: 2051 2157 2564 3587 5804 6300 2051 Size: 106 407 1023 2217 496 2352 Fragments arranged by size: 2352 2217 1023 496 407 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2570 2936 3551 1163 Size: 573 834 366 615 4213 Fragments arranged by size: 4213 834 615 573 366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2292 4051 4812 5840 1540 Size: 275 186 291 1759 761 1028 2301 Fragments arranged by size: 2301 1759 1028 761 291 275 186 BsiHKAI G_wGCw'C Cuts at: 2946 4662 4747 5818 2946 Size: 1716 85 1071 3729 Fragments arranged by size: 3729 1716 1071 85 BsmI GAATG_Cn' Cuts at: 4112 4205 4112 Size: 93 6508 BsmBI CGTCTCn'nnnn_ Cuts at: 3641 3641 Size: 6601 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2632 2946 3258 3691 3858 3920 Size: 399 303 539 314 312 433 167 62 Cuts at: 3920 4662 4747 5818 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 539 433 399 314 312 303 167 85 62 BspEI T'CCGG_A Cuts at: 6264 6264 Size: 6601 BspHI T'CATG_A Cuts at: 2220 2487 4490 6220 2220 Size: 267 2003 1730 2601 Fragments arranged by size: 2601 2003 1730 267 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2257 2956 3088 3102 3956 844 Size: 1413 699 132 14 854 3489 Fragments arranged by size: 3489 1413 854 699 132 14 BsrBI CCG'CTC Cuts at: 1394 1823 3111 3361 4488 1394 Size: 429 1288 250 1127 3507 Fragments arranged by size: 3507 1288 1127 429 250 BsrDI GCAATG_nn' Cuts at: 65 1248 2575 3534 5096 5270 65 Size: 1183 1327 959 1562 174 1396 Fragments arranged by size: 1562 1396 1327 1183 959 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2287 3119 3678 5245 5432 1700 Size: 110 477 832 559 1567 187 2869 Fragments arranged by size: 2869 1567 832 559 477 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6601 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6601 BssSI C'ACGA_G Cuts at: 4661 5673 4661 Size: 1012 5589 BstAPI GCAn_nnn'nTGC Cuts at: 794 2971 794 Size: 2177 4424 BstBI TT'CG_AA Cuts at: 2007 5405 2007 Size: 3398 3203 BstEII G'GTnAC_C Cuts at: 1184 2707 3802 1184 Size: 1523 1095 3983 Fragments arranged by size: 3983 1523 1095 BstXI CCAn_nnnn'nTGG Cuts at: 2419 2461 3199 2419 Size: 42 738 5821 Fragments arranged by size: 5821 738 42 BstYI r'GATC_y Cuts at: 1972 2032 2889 2931 3931 4298 4683 4700 6152 Size: 60 857 42 1000 367 385 17 1452 Cuts at: 6152 6238 6250 6596 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1000 857 385 367 346 86 60 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2094 2094 Size: 6601 BtgI C'CryG_G Cuts at: 513 1065 2460 3012 3104 3150 3316 3444 3849 Size: 552 1395 552 92 46 166 128 405 Cuts at: 3849 513 Size: 3265 Fragments arranged by size: 3265 1395 552 552 405 166 128 92 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2566 487 Size: 683 56 1340 4522 Fragments arranged by size: 4522 1340 683 56 BtsI GCAGTG_nn' Cuts at: 2261 2542 2695 4117 4911 4931 2261 Size: 281 153 1422 794 20 3931 Fragments arranged by size: 3931 1422 794 281 153 20 ClaI AT'CG_AT Cuts at: 1554 3285 1554 Size: 1731 4870 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6566 DraI TTT'AAA Cuts at: 3905 3905 Size: 6601 DraIII CAC_nnn'GTG Cuts at: 2133 2314 3379 2133 Size: 181 1065 5355 Fragments arranged by size: 5355 1065 181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2522 2924 5608 809 Size: 507 1206 402 2684 1802 Fragments arranged by size: 2684 1802 1206 507 402 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 2664 3117 Size: 54 1425 50 275 18 459 375 453 Cuts at: 3117 3289 4048 4937 6566 8 Size: 172 759 889 1629 43 Fragments arranged by size: 1629 1425 889 759 459 453 375 275 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2289 4048 1537 Size: 275 477 1759 4090 Fragments arranged by size: 4090 1759 477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4531 1353 Size: 3178 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2156 3104 5188 5562 5708 387 Size: 1064 138 567 948 2084 374 146 1280 Fragments arranged by size: 2084 1280 1064 948 567 374 146 138 EcoICRI GAG'CTC Cuts at: 2944 2944 Size: 6601 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2161 2210 3068 3175 3814 4664 5261 Size: 393 529 49 858 107 639 850 597 Cuts at: 5261 6048 6316 1239 Size: 787 268 1524 Fragments arranged by size: 1524 858 850 787 639 597 529 393 268 107 49 EcoO109I rG'GnC_Cy Cuts at: 2055 2055 Size: 6601 EcoRI G'AATT_C Cuts at: 2435 3910 4288 2435 Size: 1475 378 4748 Fragments arranged by size: 4748 1475 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2227 2227 Size: 6601 FspI TGC'GCA Cuts at: 5107 5107 Size: 6601 HaeII r_GCGC'y Cuts at: 1678 2428 2499 2822 2987 3198 3631 5748 1678 Size: 750 71 323 165 211 433 2117 2531 Fragments arranged by size: 2531 2117 750 433 323 211 165 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 3021 3053 5242 Size: 32 135 32 803 32 614 32 2189 Cuts at: 5242 5274 5316 5348 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2189 803 614 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3945 4191 669 Size: 988 281 912 1095 246 3079 Fragments arranged by size: 3079 1095 988 912 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6601 HpaI GTT'AAC Cuts at: 4191 4191 Size: 6601 KasI G'GCGC_C Cuts at: 2983 2983 Size: 6601 KpnI G_GTAC'C Cuts at: 3926 3926 Size: 6601 MfeI C'AATT_G Cuts at: 4200 4200 Size: 6601 MluI A'CGCG_T Cuts at: 6477 6477 Size: 6601 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2925 3052 5715 5899 1305 Size: 1620 127 2663 184 2007 Fragments arranged by size: 2663 2007 1620 184 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 3291 10 Size: 54 1425 343 834 625 3320 Fragments arranged by size: 3320 1425 834 625 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2459 2625 2645 2966 3251 3272 Size: 648 1218 75 166 20 321 285 21 Cuts at: 3272 3297 3336 3527 4559 4918 5077 518 Size: 25 39 191 1032 359 159 2042 Fragments arranged by size: 2042 1218 1032 648 359 321 285 191 166 159 75 39 25 21 20 MspA1I CmG'CkG Cuts at: 1606 2248 2557 2875 2910 3014 3106 3482 3613 Size: 642 309 318 35 104 92 376 131 Cuts at: 3613 4017 4694 5842 6087 1606 Size: 404 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 642 404 376 318 309 245 131 104 92 35 NaeI GCC'GGC Cuts at: 3121 3121 Size: 6601 NarI GG'CG_CC Cuts at: 2984 2984 Size: 6601 NcoI C'CATG_G Cuts at: 513 1065 2460 3849 513 Size: 552 1395 1389 3265 Fragments arranged by size: 3265 1395 1389 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6601 NgoMIV G'CCGG_C Cuts at: 3119 3119 Size: 6601 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6601 NotI GC'GGCC_GC Cuts at: 4048 4048 Size: 6601 NsiI A_TGCA'T Cuts at: 2652 2652 Size: 6601 NspI r_CATG'y Cuts at: 1132 1229 2650 3959 5504 1132 Size: 97 1421 1309 1545 2229 Fragments arranged by size: 2229 1545 1421 1309 97 PasI CC'CwG_GG Cuts at: 1348 3691 1348 Size: 2343 4258 PciI A'CATG_T Cuts at: 5500 5500 Size: 6601 PflMI CCAn_nnn'nTGG Cuts at: 1108 2302 2314 3228 1108 Size: 1194 12 914 4481 Fragments arranged by size: 4481 1194 914 12 PfoI T'CCnGG_A Cuts at: 3753 3753 Size: 6601 PmeI GTTT'AAAC Cuts at: 3905 3905 Size: 6601 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4670 4702 6183 6215 4670 Size: 32 1481 32 5056 Fragments arranged by size: 5056 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6601 PsiI TTA'TAA Cuts at: 4171 4171 Size: 6601 PstI C_TGCA'G Cuts at: 830 2082 2268 2537 2868 2880 2898 3099 3953 Size: 1252 186 269 331 12 18 201 854 Cuts at: 3953 830 Size: 3478 Fragments arranged by size: 3478 1252 854 331 269 201 186 18 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6601 PvuII CAG'CTG Cuts at: 2248 2557 2875 3482 3613 2248 Size: 309 318 607 131 5236 Fragments arranged by size: 5236 607 318 309 131 SacI G_AGCT'C Cuts at: 2946 2946 Size: 6601 SacII CC_GC'GG Cuts at: 3015 3107 3015 Size: 92 6509 SalI G'TCGA_C Cuts at: 1936 3943 1936 Size: 2007 4594 SbfI CC_TGCA'GG Cuts at: 3099 3953 3099 Size: 854 5747 ScaI AGT'ACT Cuts at: 4849 6561 4849 Size: 1712 4889 SexAI A'CCwGG_T Cuts at: 2922 2922 Size: 6601 SfcI C'TryA_G Cuts at: 826 1045 2078 2264 2533 2864 2876 2894 3095 Size: 219 1033 186 269 331 12 18 201 Cuts at: 3095 3949 5084 5765 5956 826 Size: 854 1135 681 191 1471 Fragments arranged by size: 1471 1135 1033 854 681 331 269 219 201 191 186 18 12 SfoI GGC'GCC Cuts at: 2985 2985 Size: 6601 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 SgrAI Cr'CCGG_yG Cuts at: 1700 3678 1700 Size: 1978 4623 SmaI CCC'GGG Cuts at: 1872 2633 3928 1872 Size: 761 1295 4545 Fragments arranged by size: 4545 1295 761 SmlI C'TyrA_G Cuts at: 820 1017 1943 4705 5606 5868 820 Size: 197 926 2762 901 262 1553 Fragments arranged by size: 2762 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3898 4392 493 Size: 3405 494 2702 Fragments arranged by size: 3405 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6601 SphI G_CATG'C Cuts at: 1132 3959 1132 Size: 2827 3774 SspI AAT'ATT Cuts at: 5 52 4525 5 Size: 47 4473 2081 Fragments arranged by size: 4473 2081 47 StuI AGG'CCT Cuts at: 1517 2041 2210 1517 Size: 524 169 5908 Fragments arranged by size: 5908 524 169 StyI C'CwwG_G Cuts at: 513 1065 2412 2460 2988 3727 3849 4040 513 Size: 552 1347 48 528 739 122 191 3074 Fragments arranged by size: 3074 1347 739 552 528 191 122 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4628 4645 4798 4983 5428 325 Size: 1255 3048 17 153 185 445 1498 Fragments arranged by size: 3048 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 4847 6559 Size: 274 80 33 51 1443 660 2210 1712 Cuts at: 6559 96 Size: 138 Fragments arranged by size: 2210 1712 1443 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6561 6593 6561 Size: 32 6569 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6601 XbaI T'CTAG_A Cuts at: 3937 3937 Size: 6601 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6601 XmaI C'CCGG_G Cuts at: 1870 2631 3926 1870 Size: 761 1295 4545 Fragments arranged by size: 4545 1295 761 XmnI GAAnn'nnTTC Cuts at: 4730 4730 Size: 6601 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2257 4409 276 Size: 53 83 186 936 723 2152 2468 Fragments arranged by size: 2468 2152 936 723 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI BplI Bpu10I BsiWI BstZ17I EcoNI EcoRV FseI FspAI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI