Restriction map for
pFN21ASDA0132
(Linear) (Six-base) MAPSORT of: KIAA0132.seq Check: 2888 from: 1 to: 1872
KIAA0132.orf
With 182 enzymes: SgfI *
February 20, 2009 16:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 933 1065 1872
Size: 933 132 807
Fragments arranged by size:
933 807 132
AatII G_ACGT'C
Cuts at: 0 236 1872
Size: 236 1636
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 1872
Size: 138 1734
AfeI AGC'GCT
Cuts at: 0 1606 1872
Size: 1606 266
AflIII A'CryG_T
Cuts at: 0 1724 1872
Size: 1724 148
AleI CACnn'nnGTG
Cuts at: 0 602 943 1313 1872
Size: 602 341 370 559
Fragments arranged by size:
602 559 370 341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 620 1551 1872
Size: 620 931 321
Fragments arranged by size:
931 620 321
AlwNI CAG_nnn'CTG
Cuts at: 0 225 534 798 1872
Size: 225 309 264 1074
Fragments arranged by size:
1074 309 264 225
ApoI r'AATT_y
Cuts at: 0 412 1872
Size: 412 1460
AvaI C'yCGr_G
Cuts at: 0 608 1872
Size: 608 1264
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1536 1569 1872
Size: 1536 33 303
Fragments arranged by size:
1536 303 33
BanI G'GyrC_C
Cuts at: 0 960 1048 1077 1872
Size: 960 88 29 795
Fragments arranged by size:
960 795 88 29
BanII G_rGCy'C
Cuts at: 0 923 1835 1872
Size: 923 912 37
Fragments arranged by size:
923 912 37
BbeI G_GCGC'C
Cuts at: 0 964 1872
Size: 964 908
BbsI GAAGACnn'nnnn_
Cuts at: 0 309 726 1872
Size: 309 417 1146
Fragments arranged by size:
1146 417 309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 534 568 822 856 1872
Size: 534 34 254 34 1016
Fragments arranged by size:
1016 534 254 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 391 1872
Size: 391 1481
BclI T'GATC_A
Cuts at: 0 1496 1872
Size: 1496 376
BfrBI ATG'CAT
Cuts at: 0 627 1872
Size: 627 1245
BglI GCCn_nnn'nGGC
Cuts at: 0 424 1097 1872
Size: 424 673 775
Fragments arranged by size:
775 673 424
BglII A'GATC_T
Cuts at: 0 908 1872
Size: 908 964
BlpI GC'TnA_GC
Cuts at: 0 214 572 1015 1872
Size: 214 358 443 857
Fragments arranged by size:
857 443 358 214
Bme1580I G_kGCm'C
Cuts at: 0 70 609 1235 1668 1872
Size: 70 539 626 433 204
Fragments arranged by size:
626 539 433 204 70
BmgBI CAC'GTC
Cuts at: 0 462 1628 1872
Size: 462 1166 244
Fragments arranged by size:
1166 462 244
BmrI ACTGGGnnnn_n'
Cuts at: 0 267 762 1034 1872
Size: 267 495 272 838
Fragments arranged by size:
838 495 272 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1045 1152 1791 1872
Size: 187 858 107 639 81
Fragments arranged by size:
858 639 187 107 81
BsaHI Gr'CG_yC
Cuts at: 0 127 233 829 961 1532 1872
Size: 127 106 596 132 571 340
Fragments arranged by size:
596 571 340 132 127 106
BsaWI w'CCGG_w
Cuts at: 0 1058 1872
Size: 1058 814
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 352 382 412 442 1150 1180 1872
Size: 352 30 30 30 708 30 692
Fragments arranged by size:
708 692 352 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 667 931 1872
Size: 667 264 941
Fragments arranged by size:
941 667 264
BseYI C'CCAG_C
Cuts at: 0 28 134 541 1564 1872
Size: 28 106 407 1023 308
Fragments arranged by size:
1023 407 308 106 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 913 1528 1872
Size: 547 366 615 344
Fragments arranged by size:
615 547 366 344
BsiEI CG_ry'CG
Cuts at: 0 269 1872
Size: 269 1603
BsiHKAI G_wGCw'C
Cuts at: 0 923 1872
Size: 923 949
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1618 1872
Size: 1618 254
Bsp1286I G_dGCh'C
Cuts at: 0 70 609 923 1235 1668 1835 1872
Size: 70 539 314 312 433 167 37
Fragments arranged by size:
539 433 314 312 167 70 37
BspHI T'CATG_A
Cuts at: 0 197 464 1872
Size: 197 267 1408
Fragments arranged by size:
1408 267 197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 234 933 1065 1079 1872
Size: 234 699 132 14 793
Fragments arranged by size:
793 699 234 132 14
BsrBI CCG'CTC
Cuts at: 0 1088 1338 1872
Size: 1088 250 534
Fragments arranged by size:
1088 534 250
BsrDI GCAATG_nn'
Cuts at: 0 552 1511 1872
Size: 552 959 361
Fragments arranged by size:
959 552 361
BsrFI r'CCGG_y
Cuts at: 0 264 1096 1655 1872
Size: 264 832 559 217
Fragments arranged by size:
832 559 264 217
BstAPI GCAn_nnn'nTGC
Cuts at: 0 948 1872
Size: 948 924
BstEII G'GTnAC_C
Cuts at: 0 684 1779 1872
Size: 684 1095 93
Fragments arranged by size:
1095 684 93
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 438 1176 1872
Size: 396 42 738 696
Fragments arranged by size:
738 696 396 42
BstYI r'GATC_y
Cuts at: 0 9 866 908 1872
Size: 9 857 42 964
Fragments arranged by size:
964 857 42 9
Bsu36I CC'TnA_GG
Cuts at: 0 71 1872
Size: 71 1801
BtgI C'CryG_G
Cuts at: 0 437 989 1081 1127 1293 1421 1826 1872
Size: 437 552 92 46 166 128 405 46
Fragments arranged by size:
552 437 405 166 128 92 46 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 543 1872
Size: 543 1329
BtsI GCAGTG_nn'
Cuts at: 0 238 519 672 1872
Size: 238 281 153 1200
Fragments arranged by size:
1200 281 238 153
ClaI AT'CG_AT
Cuts at: 0 1262 1872
Size: 1262 610
DraIII CAC_nnn'GTG
Cuts at: 0 110 291 1356 1872
Size: 110 181 1065 516
Fragments arranged by size:
1065 516 181 110
DrdI GACnn_nn'nnGTC
Cuts at: 0 499 901 1872
Size: 499 402 971
Fragments arranged by size:
971 499 402
EaeI y'GGCC_r
Cuts at: 0 266 641 1094 1266 1872
Size: 266 375 453 172 606
Fragments arranged by size:
606 453 375 266 172
EagI C'GGCC_G
Cuts at: 0 266 1872
Size: 266 1606
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 133 1081 1872
Size: 133 948 791
Fragments arranged by size:
948 791 133
EcoICRI GAG'CTC
Cuts at: 0 921 1872
Size: 921 951
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 187 1045 1152 1791 1872
Size: 138 49 858 107 639 81
Fragments arranged by size:
858 639 138 107 81 49
EcoO109I rG'GnC_Cy
Cuts at: 0 32 1872
Size: 32 1840
EcoRI G'AATT_C
Cuts at: 0 412 1872
Size: 412 1460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 1872
Size: 204 1668
HaeII r_GCGC'y
Cuts at: 0 405 476 799 964 1175 1608 1872
Size: 405 71 323 165 211 433 264
Fragments arranged by size:
433 405 323 264 211 165 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 998 1030 1872
Size: 352 32 614 32 842
Fragments arranged by size:
842 614 352 32 32
HincII GTy'rAC
Cuts at: 0 827 1872
Size: 827 1045
KasI G'GCGC_C
Cuts at: 0 960 1872
Size: 960 912
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1029 1872
Size: 902 127 843
Fragments arranged by size:
902 843 127
MscI TGG'CCA
Cuts at: 0 643 1268 1872
Size: 643 625 604
Fragments arranged by size:
643 625 604
MslI CAynn'nnrTG
Cuts at: 0 361 436 602 622 943 1228 1249 1274
Size: 361 75 166 20 321 285 21 25
Cuts at: 1274 1313 1504 1872
Size: 39 191 368
Fragments arranged by size:
368 361 321 285 191 166 75 39
25 21 20
MspA1I CmG'CkG
Cuts at: 0 225 534 852 887 991 1083 1459 1590
Size: 225 309 318 35 104 92 376 131
Cuts at: 1590 1872
Size: 282
Fragments arranged by size:
376 318 309 282 225 131 104 92
35
NaeI GCC'GGC
Cuts at: 0 1098 1872
Size: 1098 774
NarI GG'CG_CC
Cuts at: 0 961 1872
Size: 961 911
NcoI C'CATG_G
Cuts at: 0 437 1826 1872
Size: 437 1389 46
Fragments arranged by size:
1389 437 46
NgoMIV G'CCGG_C
Cuts at: 0 1096 1872
Size: 1096 776
NsiI A_TGCA'T
Cuts at: 0 629 1872
Size: 629 1243
NspI r_CATG'y
Cuts at: 0 627 1872
Size: 627 1245
PasI CC'CwG_GG
Cuts at: 0 1668 1872
Size: 1668 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 291 1205 1872
Size: 279 12 914 667
Fragments arranged by size:
914 667 279 12
PfoI T'CCnGG_A
Cuts at: 0 1730 1872
Size: 1730 142
PstI C_TGCA'G
Cuts at: 0 59 245 514 845 857 875 1076 1872
Size: 59 186 269 331 12 18 201 796
Fragments arranged by size:
796 331 269 201 186 59 18 12
PvuII CAG'CTG
Cuts at: 0 225 534 852 1459 1590 1872
Size: 225 309 318 607 131 282
Fragments arranged by size:
607 318 309 282 225 131
SacI G_AGCT'C
Cuts at: 0 923 1872
Size: 923 949
SacII CC_GC'GG
Cuts at: 0 992 1084 1872
Size: 992 92 788
Fragments arranged by size:
992 788 92
SbfI CC_TGCA'GG
Cuts at: 0 1076 1872
Size: 1076 796
SexAI A'CCwGG_T
Cuts at: 0 899 1872
Size: 899 973
SfcI C'TryA_G
Cuts at: 0 55 241 510 841 853 871 1072 1872
Size: 55 186 269 331 12 18 201 800
Fragments arranged by size:
800 331 269 201 186 55 18 12
SfoI GGC'GCC
Cuts at: 0 962 1872
Size: 962 910
SgrAI Cr'CCGG_yG
Cuts at: 0 1655 1872
Size: 1655 217
SmaI CCC'GGG
Cuts at: 0 610 1872
Size: 610 1262
StuI AGG'CCT
Cuts at: 0 18 187 1872
Size: 18 169 1685
Fragments arranged by size:
1685 169 18
StyI C'CwwG_G
Cuts at: 0 389 437 965 1704 1826 1872
Size: 389 48 528 739 122 46
Fragments arranged by size:
739 528 389 122 48 46
TatI w'GTAC_w
Cuts at: 0 614 1872
Size: 614 1258
XmaI C'CCGG_G
Cuts at: 0 608 1872
Size: 608 1264
ZraI GAC'GTC
Cuts at: 0 234 1872
Size: 234 1638
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AlwNI
ApoI AvaI BaeI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PflMI PfoI PstI PvuII SacI
SacII SbfI SexAI SfcI SfoI SgrAI SmaI StuI
StyI TatI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BamHI BbvCI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsiWI BsmI
BspEI BsrGI BssHII BssSI BstBI BstZ17I CspCI DraI
EarI EcoNI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI
SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI