Restriction map for
pFN21ASDA0106
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiWI BsmBI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MluI MluNI MroI MscI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcsI 178 GAATTT [GA]^AATT[CT]
Alw44I 138 GTGCAC G^TGCAC
ApaLI 138 GTGCAC G^TGCAC
ApoI 178 GAATTT [GA]^AATT[CT]
BamHI 351 GGATCC G^GATCC
BanI 171 GGCACC G^G[CT][GA]CC
BbsI 616 GTCTTC GAAGAC(2/6)
BbvI 160 GCTGC GCAGC(8/12)
BcnI 4 CCCGG CC^[GC]GG
BpuAI 616 GTCTTC GAAGAC(2/6)
BsaBI 313 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 118 CCTCGG C^C[ACGT][ACGT]GG
BsiEI 60 CGGCCG CG[GA][CT]^CG
BsmAI 89 GAGAC GTCTC(1/5)
BsmI 101 GCATTC GAATGC(1/-1)
BspDI 307 ATCGAT AT^CGAT
BssSI 392 CTCGTG CACGAG(-5/-1)
BstNI 242 CCTGG CC^[AT]GG
BstYI 351 GGATCC [GA]^GATC[CT]
ClaI 307 ATCGAT AT^CGAT
DdeI 481 CTCAG C^T[ACGT]AG
DpnII 352 GATC ^GATC
EaeI 60 CGGCCG [CT]^GGCC[GA]
EagI 60 CGGCCG C^GGCCG
EclXI 60 CGGCCG C^GGCCG
Eco52I 60 CGGCCG C^GGCCG
EcoRII 242 CCTGG ^CC[AT]GG
EcoRV 253 GATATC GAT^ATC
HapII 5 CCGG C^CGG
HpaII 5 CCGG C^CGG
HphI 620 TCACC GGTGA(8/7)
MaeII 26 ACGT A^CGT
MamI 313 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 352 GATC ^GATC
MfeI 267 CAATTG C^AATTG
MflI 351 GGATCC [GA]^GATC[CT]
MseI 197 TTAA T^TAA
MslI 378 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 502 CAGCTG C[AC]G^C[GT]G
MspI 5 CCGG C^CGG
MunI 267 CAATTG C^AATTG
MvaI 242 CCTGG CC^[AT]GG
MwoI 163 GCAAAGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 4 CCCGG CC^[GC]GG
NdeII 352 GATC ^GATC
PvuII 502 CAGCTG CAG^CTG
SacI 628 GAGCTC GAGCT^C
SapI 274 GAAGAGC GCTCTTC(1/4)
Sau3AI 352 GATC ^GATC
SfaNI 65 GCATC GCATC(5/9)
TaqI 308 TCGA T^CGA
Tru9I 197 TTAA T^TAA
TseI 160 GCTGC G^C[AT]GC
Tsp45I 385 GTGAC ^GT[GC]AC
TthHB8I 308 TCGA T^CGA
XhoII 351 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 63 CCGC CCGC(-3/-1)
654 CCGC
AlwI 352 GATCC GGATC(4/5)
351 GGATC
AspHI 138 GTGCAC G[AT]GC[AT]^C
628 GAGCTC
AvaI 19 CTCGGG C^[CT]CG[GA]G
119 CTCGGG
BanII 277 GAGCCC G[GA]GC[CT]^C
628 GAGCTC
BpmI 499 CTCCAG CTGGAG(16/14)
243 CTGGAG
BsiHKAI 138 GTGCAC G[AT]GC[AT]^C
628 GAGCTC
BsmFI 23 GGGAC GGGAC(10/14)
122 GGGAC
BsoBI 19 CTCGGG C^[CT]CG[GA]G
119 CTCGGG
BssKI 4 CCCGG ^CC[ACGT]GG
242 CCTGG
BstXI 332 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
532 CCAGTTGATTGG
EarI 106 CTCTTC CTCTTC(1/4)
274 GAAGAG
Fnu4HI 62 GCCGC GC^[ACGT]GC
160 GCTGC
HaeIII 45 GGCC GG^CC
61 GGCC
ItaI 62 GCCGC GC^[ACGT]GC
160 GCTGC
Ksp632I 106 CTCTTC CTCTTC(1/4)
274 GAAGAG
MaeIII 291 GTTAC ^GT[ACGT]AC
385 GTGAC
NspI 344 GCATGC [GA]CATG^[CT]
377 GCATGC
PleI 91 GACTC GAGTC(4/5)
614 GAGTC
ScrFI 4 CCCGG CC^[ACGT]GG
242 CCTGG
SphI 344 GCATGC GCATG^C
377 GCATGC
TfiI 318 GAATC G^A[AT]TC
477 GATTC
Tsp509I 179 AATT ^AATT
268 AATT
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI AvaII BmyI BsiYI BslI
Bsp1286I BsrI BstF5I Cac8I Cfr13I
FokI HinfI MboII MnlI NlaIII
NlaIV Sau96I TspRI