(Circular) (Six-base) MAPSORT of: pFN21ASDA0106.seq Check: 8646 from: 1 to: 5401 pFN21ASDA0106 5401 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3211 278 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2744 1937 Size: 807 4594 Acc65I G'GTAC_C Cuts at: 2722 2722 Size: 5401 AclI AA'CG_TT Cuts at: 1563 3528 3901 1563 Size: 1965 373 3063 Fragments arranged by size: 3063 1965 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2471 2625 3464 4848 5116 1632 Size: 839 154 839 1384 268 1917 Fragments arranged by size: 1917 1384 839 839 268 154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5204 AflIII A'CryG_T Cuts at: 1203 4300 5277 1203 Size: 3097 977 1327 Fragments arranged by size: 3097 1327 977 AgeI A'CCGG_T Cuts at: 4232 4232 Size: 5401 AhdI GACnn_n'nnGTC Cuts at: 4130 4130 Size: 5401 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5401 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5401 AlwNI CAG_nnn'CTG Cuts at: 3144 4716 3144 Size: 1572 3829 ApaLI G'TGCA_C Cuts at: 2161 3458 4614 2161 Size: 1297 1156 2948 Fragments arranged by size: 2948 1297 1156 ApoI r'AATT_y Cuts at: 1493 2201 2710 2936 3088 5197 1493 Size: 708 509 226 152 2109 1697 Fragments arranged by size: 2109 1697 708 509 226 152 AseI AT'TA_AT Cuts at: 160 1032 3955 160 Size: 872 2923 1606 Fragments arranged by size: 2923 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2142 2726 1870 Size: 73 99 100 584 4545 Fragments arranged by size: 4545 584 100 99 73 BamHI G'GATC_C Cuts at: 2374 2731 3098 2374 Size: 357 367 4677 Fragments arranged by size: 4677 367 357 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2194 2722 4177 618 Size: 325 217 627 407 528 1455 1842 Fragments arranged by size: 1842 1455 627 528 407 325 217 BanII G_rGCy'C Cuts at: 1391 2304 2655 2720 1391 Size: 913 351 65 4072 Fragments arranged by size: 4072 913 351 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2632 928 Size: 971 733 3697 Fragments arranged by size: 3697 971 733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3592 3626 1903 Size: 34 1655 34 3678 Fragments arranged by size: 3678 1655 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3293 4503 3293 Size: 1210 4191 BclI T'GATC_A Cuts at: 1548 1548 Size: 5401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4012 136 Size: 107 122 71 631 605 2340 1525 Fragments arranged by size: 2340 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5396 5396 Size: 5401 BlpI GC'TnA_GC Cuts at: 2818 2818 Size: 5401 Bme1580I G_kGCm'C Cuts at: 1790 2165 3462 4618 1790 Size: 375 1297 1156 2573 Fragments arranged by size: 2573 1297 1156 375 BmgBI CAC'GTC Cuts at: 1318 2050 1318 Size: 732 4669 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2150 2549 3132 Size: 416 53 83 399 24 733 399 583 Cuts at: 3132 4090 442 Size: 958 1753 Fragments arranged by size: 1753 958 733 583 416 399 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5401 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2287 2507 4061 1239 Size: 1048 220 1554 2579 Fragments arranged by size: 2579 1554 1048 220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3526 4391 4689 3526 Size: 865 298 4238 Fragments arranged by size: 4238 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4064 882 Size: 617 2565 2219 Fragments arranged by size: 2565 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2698 3192 493 Size: 711 1494 494 2702 Fragments arranged by size: 2702 1494 711 494 BsaBI GATnn'nnATC Cuts at: 2340 5395 2340 Size: 3055 2346 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3208 3590 4225 275 Size: 53 83 186 936 1675 382 635 1451 Fragments arranged by size: 1675 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3834 4232 4506 4653 5064 3834 Size: 398 274 147 411 4171 Fragments arranged by size: 4171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5176 Fragments arranged by size: 5176 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5401 BseYI C'CCAG_C Cuts at: 2470 2688 4604 5100 2470 Size: 218 1916 496 2771 Fragments arranged by size: 2771 1916 496 218 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4828 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2086 2851 3612 4640 1540 Size: 275 186 85 765 761 1028 2301 Fragments arranged by size: 2301 1028 765 761 275 186 85 BsiHKAI G_wGCw'C Cuts at: 2165 2655 3462 3547 4618 2165 Size: 490 807 85 1071 2948 Fragments arranged by size: 2948 1071 807 490 85 BsmI GAATG_Cn' Cuts at: 2124 2912 3005 2124 Size: 788 93 4520 Fragments arranged by size: 4520 788 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2165 2304 2655 2720 3462 3547 4618 Size: 399 375 139 351 65 742 85 1071 Cuts at: 4618 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 375 351 139 85 65 BspEI T'CCGG_A Cuts at: 5064 5064 Size: 5401 BspHI T'CATG_A Cuts at: 3290 5020 3290 Size: 1730 3671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2756 844 Size: 1912 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3288 1394 Size: 429 1465 3507 Fragments arranged by size: 3507 1465 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3896 4070 65 Size: 1183 2648 174 1396 Fragments arranged by size: 2648 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4045 4232 1700 Size: 110 2235 187 2869 Fragments arranged by size: 2869 2235 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5401 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5401 BssSI C'ACGA_G Cuts at: 2415 3461 4473 2415 Size: 1046 1012 3343 Fragments arranged by size: 3343 1046 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5401 BstBI TT'CG_AA Cuts at: 2007 4205 2007 Size: 2198 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5401 BstXI CCAn_nnnn'nTGG Cuts at: 2362 2562 2362 Size: 200 5201 BstYI r'GATC_y Cuts at: 1972 2374 2731 3098 3483 3500 4952 5038 5050 Size: 402 357 367 385 17 1452 86 12 Cuts at: 5050 5396 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 402 385 367 357 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4849 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4662 Fragments arranged by size: 4662 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3711 3731 2917 Size: 794 20 4587 Fragments arranged by size: 4587 794 20 ClaI AT'CG_AT Cuts at: 1554 2331 1554 Size: 777 4624 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2155 2190 2539 2574 529 Size: 35 1591 35 349 35 3356 Fragments arranged by size: 3356 1591 349 35 35 35 DraI TTT'AAA Cuts at: 2705 2705 Size: 5401 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4408 809 Size: 507 3092 1802 Fragments arranged by size: 3092 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2848 3737 Size: 54 1425 50 275 18 253 765 889 Cuts at: 3737 5366 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 765 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2083 2848 1537 Size: 275 271 765 4090 Fragments arranged by size: 4090 765 275 271 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 2292 3331 1353 Size: 782 157 1039 3423 Fragments arranged by size: 3423 1039 782 157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3988 4362 4508 387 Size: 1064 138 2399 374 146 1280 Fragments arranged by size: 2399 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2653 2653 Size: 5401 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2287 2471 2507 2625 3464 4061 4848 Size: 393 655 184 36 118 839 597 787 Cuts at: 4848 5116 1239 Size: 268 1524 Fragments arranged by size: 1524 839 787 655 597 393 268 184 118 36 EcoRI G'AATT_C Cuts at: 2710 3088 2710 Size: 378 5023 EcoRV GAT'ATC Cuts at: 2278 2278 Size: 5401 FspI TGC'GCA Cuts at: 3907 3907 Size: 5401 HaeII r_GCGC'y Cuts at: 1678 4548 1678 Size: 2870 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2642 2674 4042 4074 4116 Size: 32 135 32 1070 32 1368 32 42 Cuts at: 4116 4148 1373 Size: 32 2626 Fragments arranged by size: 2626 1368 1070 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2745 2991 669 Size: 988 281 807 246 3079 Fragments arranged by size: 3079 988 807 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5401 HpaI GTT'AAC Cuts at: 2991 2991 Size: 5401 KpnI G_GTAC'C Cuts at: 2726 2726 Size: 5401 MfeI C'AATT_G Cuts at: 2290 3000 2290 Size: 710 4691 MluI A'CGCG_T Cuts at: 5277 5277 Size: 5401 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2618 4515 4699 1305 Size: 1313 1897 184 2007 Fragments arranged by size: 2007 1897 1313 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3579 Fragments arranged by size: 3579 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2405 3359 3718 3877 518 Size: 648 1239 954 359 159 2042 Fragments arranged by size: 2042 1239 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2527 2817 3494 4642 4887 1606 Size: 921 290 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 677 290 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4849 NdeI CA'TA_TG Cuts at: 387 387 Size: 5401 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5401 NotI GC'GGCC_GC Cuts at: 2848 2848 Size: 5401 NspI r_CATG'y Cuts at: 1132 1229 2371 2404 2759 4304 1132 Size: 97 1142 33 355 1545 2229 Fragments arranged by size: 2229 1545 1142 355 97 33 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5401 PciI A'CATG_T Cuts at: 4300 4300 Size: 5401 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5401 PmeI GTTT'AAAC Cuts at: 2705 2705 Size: 5401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3470 3502 4983 5015 3470 Size: 32 1481 32 3856 Fragments arranged by size: 3856 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5401 PsiI TTA'TAA Cuts at: 2971 2971 Size: 5401 PstI C_TGCA'G Cuts at: 830 2753 830 Size: 1923 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5401 PvuII CAG'CTG Cuts at: 2527 2527 Size: 5401 SacI G_AGCT'C Cuts at: 2655 2655 Size: 5401 SalI G'TCGA_C Cuts at: 1936 2743 1936 Size: 807 4594 SapI GCTCTTCn'nnn_ Cuts at: 2292 2292 Size: 5401 SbfI CC_TGCA'GG Cuts at: 2753 2753 Size: 5401 ScaI AGT'ACT Cuts at: 3649 5361 3649 Size: 1712 3689 SfcI C'TryA_G Cuts at: 826 1045 2749 3884 4565 4756 826 Size: 219 1704 1135 681 191 1471 Fragments arranged by size: 1704 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5401 SmaI CCC'GGG Cuts at: 1872 2728 1872 Size: 856 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3505 4406 4668 820 Size: 197 926 1562 901 262 1553 Fragments arranged by size: 1562 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2698 3192 493 Size: 2205 494 2702 Fragments arranged by size: 2702 2205 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5401 SphI G_CATG'C Cuts at: 1132 2371 2404 2759 1132 Size: 1239 33 355 3774 Fragments arranged by size: 3774 1239 355 33 SspI AAT'ATT Cuts at: 5 52 3325 5 Size: 47 3273 2081 Fragments arranged by size: 3273 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5401 StyI C'CwwG_G Cuts at: 513 1065 2840 513 Size: 552 1775 3074 Fragments arranged by size: 3074 1775 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3428 3445 3598 3783 4228 325 Size: 1255 1848 17 153 185 445 1498 Fragments arranged by size: 1848 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3647 5359 96 Size: 274 80 33 51 1443 1670 1712 138 Fragments arranged by size: 1712 1670 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5361 5393 5361 Size: 32 5369 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5401 XbaI T'CTAG_A Cuts at: 2737 2737 Size: 5401 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5401 XmaI C'CCGG_G Cuts at: 1870 2726 1870 Size: 856 4545 XmnI GAAnn'nnTTC Cuts at: 3530 3530 Size: 5401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3209 276 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI