Restriction map for
pFN21ASDA0106
(Linear) (Six-base) MAPSORT of: KIAA0106.seq Check: 6964 from: 1 to: 672
KIAA0106.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 602 672
Size: 448 154 70
Fragments arranged by size:
448 154 70
ApaLI G'TGCA_C
Cuts at: 0 138 672
Size: 138 534
ApoI r'AATT_y
Cuts at: 0 178 672
Size: 178 494
AvaI C'yCGr_G
Cuts at: 0 19 119 672
Size: 19 100 553
Fragments arranged by size:
553 100 19
BamHI G'GATC_C
Cuts at: 0 351 672
Size: 351 321
BanI G'GyrC_C
Cuts at: 0 171 672
Size: 171 501
BanII G_rGCy'C
Cuts at: 0 281 632 672
Size: 281 351 40
Fragments arranged by size:
351 281 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 609 672
Size: 609 63
Bme1580I G_kGCm'C
Cuts at: 0 142 672
Size: 142 530
BmgBI CAC'GTC
Cuts at: 0 27 672
Size: 27 645
BmrI ACTGGGnnnn_n'
Cuts at: 0 127 526 672
Size: 127 399 146
Fragments arranged by size:
399 146 127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 484 672
Size: 264 220 188
Fragments arranged by size:
264 220 188
BsaBI GATnn'nnATC
Cuts at: 0 317 672
Size: 317 355
BseYI C'CCAG_C
Cuts at: 0 447 665 672
Size: 447 218 7
Fragments arranged by size:
447 218 7
BsiEI CG_ry'CG
Cuts at: 0 63 672
Size: 63 609
BsiHKAI G_wGCw'C
Cuts at: 0 142 632 672
Size: 142 490 40
Fragments arranged by size:
490 142 40
BsmI GAATG_Cn'
Cuts at: 0 101 672
Size: 101 571
Bsp1286I G_dGCh'C
Cuts at: 0 142 281 632 672
Size: 142 139 351 40
Fragments arranged by size:
351 142 139 40
BssSI C'ACGA_G
Cuts at: 0 392 672
Size: 392 280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 539 672
Size: 339 200 133
Fragments arranged by size:
339 200 133
BstYI r'GATC_y
Cuts at: 0 351 672
Size: 351 321
ClaI AT'CG_AT
Cuts at: 0 308 672
Size: 308 364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 132 167 516 551 672
Size: 132 35 349 35 121
Fragments arranged by size:
349 132 121 35 35
EaeI y'GGCC_r
Cuts at: 0 60 672
Size: 60 612
EagI C'GGCC_G
Cuts at: 0 60 672
Size: 60 612
EarI CTCTTCn'nnn_
Cuts at: 0 112 269 672
Size: 112 157 403
Fragments arranged by size:
403 157 112
EcoICRI GAG'CTC
Cuts at: 0 630 672
Size: 630 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 448 484 602 672
Size: 264 184 36 118 70
Fragments arranged by size:
264 184 118 70 36
EcoRV GAT'ATC
Cuts at: 0 255 672
Size: 255 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 672
Size: 619 32 21
Fragments arranged by size:
619 32 21
MfeI C'AATT_G
Cuts at: 0 267 672
Size: 267 405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 672
Size: 595 77
MslI CAynn'nnrTG
Cuts at: 0 382 672
Size: 382 290
MspA1I CmG'CkG
Cuts at: 0 504 672
Size: 504 168
NspI r_CATG'y
Cuts at: 0 348 381 672
Size: 348 33 291
Fragments arranged by size:
348 291 33
PvuII CAG'CTG
Cuts at: 0 504 672
Size: 504 168
SacI G_AGCT'C
Cuts at: 0 632 672
Size: 632 40
SapI GCTCTTCn'nnn_
Cuts at: 0 269 672
Size: 269 403
SphI G_CATG'C
Cuts at: 0 348 381 672
Size: 348 33 291
Fragments arranged by size:
348 291 33
Enzymes that do cut:
AcuI ApaLI ApoI AvaI BamHI BanI BanII BbsI
Bme1580I BmgBI BmrI BpmI BsaBI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BssSI BstXI BstYI ClaI CspCI EaeI
EagI EarI EcoICRI Eco57MI EcoRV Hin4I MfeI MmeI
MslI MspA1I NspI PvuII SacI SapI SphI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EciI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI