Restriction map for pFN21AA0102
 (Circular) (Six-base) MAPSORT of: pFN21AA0102.seq  Check: 5412  from: 1  to: 5383



pFN21AA0102  5383 bp

 With 182 enzymes: SgfI * 

                        January 31, 2011 17:47  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3193     278 
   Size:         53      83     186     936    1657    2468

  Fragments arranged by size: 

                2468    1657     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2726    1937 
   Size:        789    4594
 
Acc65I G'GTAC_C

Cuts at:   2704    2704 
   Size:       5383
 
AclI AA'CG_TT

Cuts at:   1563    3510    3883    1563 
   Size:       1947     373    3063

  Fragments arranged by size: 

                3063    1947     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3446    4830    5098    1632 
   Size:       1814    1384     268    1917

  Fragments arranged by size: 

                1917    1814    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5186
 
AflIII A'CryG_T

Cuts at:   1203    4282    5259    1203 
   Size:       3079     977    1327

  Fragments arranged by size: 

                3079    1327     977
 
AgeI A'CCGG_T

Cuts at:   4214    4214 
   Size:       5383
 
AhdI GACnn_n'nnGTC

Cuts at:   4112    4112 
   Size:       5383
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5383
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5383
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2552    2584    2552 
   Size:         32    5351
 
AlwNI CAG_nnn'CTG

Cuts at:   3126    4698    3126 
   Size:       1572    3811
 
ApaLI G'TGCA_C

Cuts at:   3440    4596    3440 
   Size:       1156    4227
 
ApoI r'AATT_y

Cuts at:   1493    2233    2692    2918    3070    5179    1493 
   Size:        740     459     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     740     459     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3937     160 
   Size:        872    2905    1606

  Fragments arranged by size: 

                2905    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5383
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2708    1870 
   Size:         73     765    4545

  Fragments arranged by size: 

                4545     765      73
 
BamHI G'GATC_C

Cuts at:   2458    2713    3080    2458 
   Size:        255     367    4761

  Fragments arranged by size: 

                4761     367     255
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2704    4159     618 
   Size:        325     217     627     917    1455    1842

  Fragments arranged by size: 

                1842    1455     917     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2702    1391 
   Size:       1311    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4412
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2407    2441    3574    3608    1903 
   Size:         34     470      34    1133      34    3678

  Fragments arranged by size: 

                3678    1133     470      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3275    4485    3275 
   Size:       1210    4173
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5383
 
BfrBI ATG'CAT

Cuts at:   2624    2624 
   Size:       5383
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2103    2294 
   Size:        107     122      71     631     605     337      94     191

Cuts at:   2294    3994     136 
   Size:       1700    1525

  Fragments arranged by size: 

                1700    1525     631     605     337     191     122     107
                  94      71
 
BglII A'GATC_T

Cuts at:   5378    5378 
   Size:       5383
 
BlpI GC'TnA_GC

Cuts at:   2800    2800 
   Size:       5383
 
Bme1580I G_kGCm'C

Cuts at:   1790    3444    4600    1790 
   Size:       1654    1156    2573

  Fragments arranged by size: 

                2573    1654    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5383
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3114    4072     442 
   Size:        416      53      83     399      24    1697     958    1753

  Fragments arranged by size: 

                1753    1697     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5383
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2470    4043    1239 
   Size:       1231    1573    2579

  Fragments arranged by size: 

                2579    1573    1231
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3508    4373    4671    3508 
   Size:        865     298    4220

  Fragments arranged by size: 

                4220     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4046     882 
   Size:        617    2547    2219

  Fragments arranged by size: 

                2547    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3174     493 
   Size:        711    1970    2702

  Fragments arranged by size: 

                2702    1970     711
 
BsaBI GATnn'nnATC

Cuts at:   5377    5377 
   Size:       5383
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3190    3572    4207     275 
   Size:         53      83     186     936    1657     382     635    1451

  Fragments arranged by size: 

                1657    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3816    4214    4488    4635    5046    3816 
   Size:        398     274     147     411    4153

  Fragments arranged by size: 

                4153     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2023    2053    1178 
   Size:         30     165      30     620      30    4508

  Fragments arranged by size: 

                4508     620     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5383
 
BseYI C'CCAG_C

Cuts at:   4586    5082    4586 
   Size:        496    4887
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4810
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2833    3594    4622    1540 
   Size:        275     186     832     761    1028    2301

  Fragments arranged by size: 

                2301    1028     832     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3444    3529    4600    3444 
   Size:         85    1071    4227

  Fragments arranged by size: 

                4227    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2894    2987    2894 
   Size:         93    5290
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2702    3444    3529    4600    1391 
   Size:        399     912     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     912     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5046    5046 
   Size:       5383
 
BspHI T'CATG_A

Cuts at:   3272    5002    3272 
   Size:       1730    3653
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2738     844 
   Size:       1894    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3270    1394 
   Size:        429    1447    3507

  Fragments arranged by size: 

                3507    1447     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2580    3878    4052      65 
   Size:       1183    1332    1298     174    1396

  Fragments arranged by size: 

                1396    1332    1298    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4027    4214    1700 
   Size:        110    2217     187    2869

  Fragments arranged by size: 

                2869    2217     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2020    2267      96 
   Size:       1924     247    3212

  Fragments arranged by size: 

                3212    1924     247
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5383
 
BssSI C'ACGA_G

Cuts at:   2426    3443    4455    2426 
   Size:       1017    1012    3354

  Fragments arranged by size: 

                3354    1017    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5383
 
BstBI TT'CG_AA

Cuts at:   4187    4187 
   Size:       5383
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5383
 
BstYI r'GATC_y

Cuts at:   1972    2443    2458    2713    3080    3465    3482    4934    5020 
   Size:        471      15     255     367     385      17    1452      86

Cuts at:   5020    5032    5378    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     471     385     367     346     255      86
                  17      15      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3892

  Fragments arranged by size: 

                3892     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4644

  Fragments arranged by size: 

                4644     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2899    3693    3713    2899 
   Size:        794      20    4569

  Fragments arranged by size: 

                4569     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5383
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5348
 
DraI TTT'AAA

Cuts at:   2687    2687 
   Size:       5383
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4390     809 
   Size:        507    3074    1802

  Fragments arranged by size: 

                3074    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2095    2830    3719 
   Size:         54    1425      50     275      18     265     735     889

Cuts at:   3719    5348       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     735     275     265      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2830    1537 
   Size:        275    1018    4090

  Fragments arranged by size: 

                4090    1018     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2408    3313    1353 
   Size:       1055     905    3423

  Fragments arranged by size: 

                3423    1055     905
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3970    4344    4490     387 
   Size:       1064     138    2381     374     146    1280

  Fragments arranged by size: 

                2381    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2470    3446    4043    4830    5098    1239 
   Size:        393     838     976     597     787     268    1524

  Fragments arranged by size: 

                1524     976     838     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2692    3070    2692 
   Size:        378    5005
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2437    2545    2437 
   Size:        108    5275
 
FspI TGC'GCA

Cuts at:   3889    3889 
   Size:       5383
 
HaeII r_GCGC'y

Cuts at:   1678    4530    1678 
   Size:       2852    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4024    4056    4098    4130    1373 
   Size:         32     135      32    2452      32      42      32    2626

  Fragments arranged by size: 

                2626    2452     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2727    2973     669 
   Size:        988     281     789     246    3079

  Fragments arranged by size: 

                3079     988     789     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5383
 
HpaI GTT'AAC

Cuts at:   2973    2973 
   Size:       5383
 
KpnI G_GTAC'C

Cuts at:   2708    2708 
   Size:       5383
 
MfeI C'AATT_G

Cuts at:   2982    2982 
   Size:       5383
 
MluI A'CGCG_T

Cuts at:   5259    5259 
   Size:       5383
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2091    2100    4497    4681    1305 
   Size:        786       9    2397     184    2007

  Fragments arranged by size: 

                2397    2007     786     184       9
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3561

  Fragments arranged by size: 

                3561    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3341    3700    3859     518 
   Size:        648    2175     359     159    2042

  Fragments arranged by size: 

                2175    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2018    2168    2479    2557    2799    3476    4624    4869 
   Size:        412     150     311      78     242     677    1148     245

Cuts at:   4869    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     412     311     245     242     150
                  78
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3892

  Fragments arranged by size: 

                3892     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2626     387 
   Size:       2239    3144
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5383
 
NotI GC'GGCC_GC

Cuts at:   2830    2830 
   Size:       5383
 
NsiI A_TGCA'T

Cuts at:   2626    2626 
   Size:       5383
 
NspI r_CATG'y

Cuts at:   1132    1229    2741    4286    1132 
   Size:         97    1512    1545    2229

  Fragments arranged by size: 

                2229    1545    1512      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5383
 
PciI A'CATG_T

Cuts at:   4282    4282 
   Size:       5383
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5383
 
PmeI GTTT'AAAC

Cuts at:   2687    2687 
   Size:       5383
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3452    3484    4965    4997    3452 
   Size:         32    1481      32    3838

  Fragments arranged by size: 

                3838    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2612    1613 
   Size:        999    4384
 
PsiI TTA'TAA

Cuts at:   2953    2953 
   Size:       5383
 
PstI C_TGCA'G

Cuts at:    830    2735     830 
   Size:       1905    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5383
 
PvuII CAG'CTG

Cuts at:   2018    2168    2479    2018 
   Size:        150     311    4922

  Fragments arranged by size: 

                4922     311     150
 
SalI G'TCGA_C

Cuts at:   1936    2725    1936 
   Size:        789    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2408    2408 
   Size:       5383
 
SbfI CC_TGCA'GG

Cuts at:   2735    2735 
   Size:       5383
 
ScaI AGT'ACT

Cuts at:   3631    5343    3631 
   Size:       1712    3671
 
SfcI C'TryA_G

Cuts at:    826    1045    2053    2448    2731    3866    4547    4738     826 
   Size:        219    1008     395     283    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1008     681     395     283     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5383
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5383
 
SmaI CCC'GGG

Cuts at:   1872    2710    1872 
   Size:        838    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3487    4388    4650     820 
   Size:        197     926    1544     901     262    1553

  Fragments arranged by size: 

                1553    1544     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3174     493 
   Size:       2681    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5383
 
SphI G_CATG'C

Cuts at:   1132    2741    1132 
   Size:       1609    3774
 
SspI AAT'ATT

Cuts at:      5      52    3307       5 
   Size:         47    3255    2081

  Fragments arranged by size: 

                3255    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5383
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2822     513 
   Size:        552     939     818    3074

  Fragments arranged by size: 

                3074     939     818     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3410    3427    3580    3765    4210     325 
   Size:       1255    1830      17     153     185     445    1498

  Fragments arranged by size: 

                1830    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2020    2267    3629 
   Size:        274      80      33      51    1443      43     247    1362

Cuts at:   3629    5341      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1362     274     247     138      80      51
                  43      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2023    2055    2077    2109    2154    2186    5343    5375    2023 
   Size:         32      22      32      45      32    3157      32    2031

  Fragments arranged by size: 

                3157    2031      45      32      32      32      32      22
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5383
 
XbaI T'CTAG_A

Cuts at:   2719    2719 
   Size:       5383
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2341    2341 
   Size:       5383
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5383
 
XmaI C'CCGG_G

Cuts at:   1870    2708    1870 
   Size:        838    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3512    3512 
   Size:       5383
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3191     276 
   Size:         53      83     186     936    1657    2468

  Fragments arranged by size: 

                2468    1657     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NsiI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     PacI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI    SexAI     SfiI
     SfoI     SrfI     SwaI
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