Restriction map for
pFN21ASDA0096
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
AspEI AvrII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FseI HaeII
HpaI KasI KspI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1861 GGTACC G^GTACC
AccII 1152 CGCG CG^CG
AcyI 931 GACGCC G[GA]^CG[CT]C
AocI 2242 CCTAAGG CC^T[ACGT]AGG
ApaI 1714 GGGCCC GGGCC^C
Asp700I 767 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1861 GGTACC G^GTACC
AspI 1186 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2260 TGCGCA TGC^GCA
BamHI 1004 GGATCC G^GATCC
BclI 677 TGATCA T^GATCA
BcnI 1599 CCGGG CC^[GC]GG
BsaAI 2049 TACGTG [CT]AC^GT[GA]
BsaBI 1504 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 931 GACGCC G[GA]^CG[CT]C
BsaI 1624 GAGACC GGTCTC(1/5)
BsaWI 1580 ACCGGA [AT]^CCGG[AT]
BsgI 208 GTGCAG GTGCAG(16/14)
BsmBI 929 GAGACG CGTCTC(1/5)
BsmI 2079 GAATGC GAATGC(1/-1)
BspHI 1946 TCATGA T^CATGA
BsrBI 542 CCGCTC CCGCTC(-3/-3)
BsrDI 1760 GCAATG GCAATG(2/0)
BstUI 1152 CGCG CG^CG
Bsu36I 2242 CCTAAGG CC^T[ACGT]AGG
DsaI 83 CCGTGG C^C[GA][CT]GG
Eco81I 2242 CCTAAGG CC^T[ACGT]AGG
EcoT22I 195 ATGCAT ATGCA^T
FbaI 677 TGATCA T^GATCA
FspI 2260 TGCGCA TGC^GCA
HgaI 931 GACGC GACGC(5/10)
Hin1I 931 GACGCC G[GA]^CG[CT]C
HincII 465 GTTGAC GT[CT]^[GA]AC
HindII 465 GTTGAC GT[CT]^[GA]AC
HindIII 1290 AAGCTT A^AGCTT
KpnI 1861 GGTACC GGTAC^C
MamI 1504 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1152 CGCG CG^CG
NciI 1599 CCGGG CC^[GC]GG
NsiI 195 ATGCAT ATGCA^T
NspI 102 GCATGT [GA]CATG^[CT]
PflFI 1186 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2002 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1946 TCATGA T^CATGA
SacI 1924 GAGCTC GAGCT^C
SmlI 1117 CTTGAG C^T[CT][GA]AG
SpeI 204 ACTAGT A^CTAGT
StuI 1215 AGGCCT AGG^CCT
Tsp45I 1223 GTGAC ^GT[GC]AC
Tth111I 1186 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2002 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 273 TCTAGA T^CTAGA
XmnI 767 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 490 AAATTT [GA]^AATT[CT]
551 AAATTC
ApoI 490 AAATTT [GA]^AATT[CT]
551 AAATTC
BbsI 337 GAAGAC GAAGAC(2/6)
436 GTCTTC
BglII 1042 AGATCT A^GATCT
1463 AGATCT
BpuAI 337 GAAGAC GAAGAC(2/6)
436 GTCTTC
BsrFI 1649 GCCGGC [GA]^CCGG[CT]
1879 GCCGGC
Cfr10I 1649 GCCGGC [GA]^CCGG[CT]
1879 GCCGGC
EcoT14I 62 CCTTGG C^C[AT][AT]GG
799 CCTTGG
MaeII 2050 ACGT A^CGT
2186 ACGT
MaeIII 1223 GTGAC ^GT[ACGT]AC
1417 GTAAC
NaeI 1649 GCCGGC GCC^GGC
1879 GCCGGC
NgoMIV 1649 GCCGGC G^CCGGC
1879 GCCGGC
PleI 1225 GACTC GAGTC(4/5)
597 GAGTC
PpuMI 1112 AGGACCT [GA]G^G[AT]CC[CT]
2090 AGGTCCC
StyI 62 CCTTGG C^C[AT][AT]GG
799 CCTTGG
XcmI 1766 CCAGTGGAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2141 CCACCACTGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII