(Circular) (Six-base) MAPSORT of: pFN21ASDA0096.seq Check: 5073 from: 1 to: 7024 pFN21ASDA0096 7024 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2611 2611 Size: 7024 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4834 278 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4367 1937 Size: 2430 4594 Acc65I G'GTAC_C Cuts at: 3884 4345 3884 Size: 461 6563 AclI AA'CG_TT Cuts at: 1563 5151 5524 1563 Size: 3588 373 3063 Fragments arranged by size: 3588 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2813 5087 6471 6739 1632 Size: 1181 2274 1384 268 1917 Fragments arranged by size: 2274 1917 1384 1181 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6827 AflIII A'CryG_T Cuts at: 1203 5923 6900 1203 Size: 4720 977 1327 Fragments arranged by size: 4720 1327 977 AgeI A'CCGG_T Cuts at: 5855 5855 Size: 7024 AhdI GACnn_n'nnGTC Cuts at: 5753 5753 Size: 7024 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7024 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3935 4134 1184 Size: 2751 199 4074 Fragments arranged by size: 4074 2751 199 AlwNI CAG_nnn'CTG Cuts at: 4767 6339 4767 Size: 1572 5452 ApaI G_GGCC'C Cuts at: 3741 3741 Size: 7024 ApaLI G'TGCA_C Cuts at: 5081 6237 5081 Size: 1156 5868 ApoI r'AATT_y Cuts at: 1493 2513 2574 4333 4559 4711 6820 1493 Size: 1020 61 1759 226 152 2109 1697 Fragments arranged by size: 2109 1759 1697 1020 226 152 61 AseI AT'TA_AT Cuts at: 160 1032 5578 160 Size: 872 4546 1606 Fragments arranged by size: 4546 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 AvaI C'yCGr_G Cuts at: 1870 1943 3076 3198 3856 4349 1870 Size: 73 1133 122 658 493 4545 Fragments arranged by size: 4545 1133 658 493 122 73 BamHI G'GATC_C Cuts at: 3027 4354 4721 3027 Size: 1327 367 5330 Fragments arranged by size: 5330 1327 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3301 3714 3884 4345 5800 Size: 325 217 627 1514 413 170 461 1455 Cuts at: 5800 618 Size: 1842 Fragments arranged by size: 1842 1514 1455 627 461 413 325 217 170 BanII G_rGCy'C Cuts at: 1391 3629 3741 3951 4343 1391 Size: 2238 112 210 392 4072 Fragments arranged by size: 4072 2238 392 210 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2367 2452 928 Size: 971 468 85 5500 Fragments arranged by size: 5500 971 468 85 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5215 5249 1903 Size: 34 3278 34 3678 Fragments arranged by size: 3678 3278 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4916 6126 4916 Size: 1210 5814 BclI T'GATC_A Cuts at: 1548 2700 1548 Size: 1152 5872 BfrBI ATG'CAT Cuts at: 2220 2220 Size: 7024 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5635 136 Size: 107 122 71 631 605 3963 1525 Fragments arranged by size: 3963 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3065 3486 7019 3065 Size: 421 3533 3070 Fragments arranged by size: 3533 3070 421 BlpI GC'TnA_GC Cuts at: 4441 4441 Size: 7024 Bme1580I G_kGCm'C Cuts at: 1790 3741 5085 6241 1790 Size: 1951 1344 1156 2573 Fragments arranged by size: 2573 1951 1344 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7024 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3088 3810 4755 Size: 416 53 83 399 24 1671 722 945 Cuts at: 4755 5713 442 Size: 958 1753 Fragments arranged by size: 1753 1671 958 945 722 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7024 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2553 3561 3613 3832 5684 1239 Size: 1314 1008 52 219 1852 2579 Fragments arranged by size: 2579 1852 1314 1008 219 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3161 5149 6014 6312 3161 Size: 1988 865 298 3873 Fragments arranged by size: 3873 1988 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3641 5687 882 Size: 617 2142 2046 2219 Fragments arranged by size: 2219 2142 2046 617 BsaAI yAC'GTr Cuts at: 493 1204 4074 4321 4815 493 Size: 711 2870 247 494 2702 Fragments arranged by size: 2870 2702 711 494 247 BsaBI GATnn'nnATC Cuts at: 3531 7018 3531 Size: 3487 3537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2955 4831 5213 5848 Size: 53 83 186 936 1422 1876 382 635 Cuts at: 5848 275 Size: 1451 Fragments arranged by size: 1876 1451 1422 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3603 5457 5855 6129 6276 6687 3603 Size: 1854 398 274 147 411 3940 Fragments arranged by size: 3940 1854 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3145 3175 3598 3628 1178 Size: 30 165 30 1742 30 423 30 4574 Fragments arranged by size: 4574 1742 423 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2328 2360 2915 3395 1186 Size: 1142 32 555 480 4815 Fragments arranged by size: 4815 1142 555 480 32 BseYI C'CCAG_C Cuts at: 3908 3941 3990 4294 6227 6723 3908 Size: 33 49 304 1933 496 4209 Fragments arranged by size: 4209 1933 496 304 49 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 1163 Size: 573 516 5935 Fragments arranged by size: 5935 573 516 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4474 5235 6263 1540 Size: 275 186 2473 761 1028 2301 Fragments arranged by size: 2473 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2907 3951 4007 5085 5170 6241 2907 Size: 1044 56 1078 85 1071 3690 Fragments arranged by size: 3690 1078 1071 1044 85 56 BsmI GAATG_Cn' Cuts at: 4108 4535 4628 4108 Size: 427 93 6504 Fragments arranged by size: 6504 427 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2946 2946 Size: 7024 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2907 3629 3741 3951 4007 4343 5085 Size: 399 1117 722 112 210 56 336 742 Cuts at: 5085 5170 6241 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1117 1071 742 722 399 336 210 112 85 56 BspEI T'CCGG_A Cuts at: 6687 6687 Size: 7024 BspHI T'CATG_A Cuts at: 3969 4913 6643 3969 Size: 944 1730 4350 Fragments arranged by size: 4350 1730 944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2611 3543 4102 4379 844 Size: 1767 932 559 277 3489 Fragments arranged by size: 3489 1767 932 559 277 BsrBI CCG'CTC Cuts at: 1394 1823 2567 4911 1394 Size: 429 744 2344 3507 Fragments arranged by size: 3507 2344 744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3790 5519 5693 65 Size: 1183 2542 1729 174 1396 Fragments arranged by size: 2542 1729 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3672 3902 5668 5855 1700 Size: 110 1862 230 1766 187 2869 Fragments arranged by size: 2869 1862 1766 230 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7024 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7024 BssSI C'ACGA_G Cuts at: 5084 6096 5084 Size: 1012 6012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7024 BstBI TT'CG_AA Cuts at: 2007 5828 2007 Size: 3821 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7024 BstXI CCAn_nnnn'nTGG Cuts at: 3925 3998 4174 3925 Size: 73 176 6775 Fragments arranged by size: 6775 176 73 BstYI r'GATC_y Cuts at: 1972 2549 2779 3027 3065 3486 4354 4721 5106 Size: 577 230 248 38 421 868 367 385 Cuts at: 5106 5123 6575 6661 6673 7019 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 868 577 421 385 367 346 248 230 86 38 17 12 Bsu36I CC'TnA_GG Cuts at: 4266 4266 Size: 7024 BtgI C'CryG_G Cuts at: 513 1065 2022 2106 513 Size: 552 957 84 5431 Fragments arranged by size: 5431 957 552 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6285 Fragments arranged by size: 6285 683 56 BtsI GCAGTG_nn' Cuts at: 3754 3943 4128 4540 5334 5354 3754 Size: 189 185 412 794 20 5424 Fragments arranged by size: 5424 794 412 189 185 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7024 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3396 3431 3772 3807 3814 3849 529 Size: 35 2832 35 341 35 7 35 3704 Fragments arranged by size: 3704 2832 341 35 35 35 35 7 DraI TTT'AAA Cuts at: 4328 4328 Size: 7024 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6031 809 Size: 507 4715 1802 Fragments arranged by size: 4715 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2367 3110 3156 Size: 54 1425 50 275 18 537 743 46 Cuts at: 3156 3223 4471 5360 6989 8 Size: 67 1248 889 1629 43 Fragments arranged by size: 1629 1425 1248 889 743 537 275 67 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4471 1537 Size: 275 2659 4090 Fragments arranged by size: 4090 2659 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2799 2892 3363 3458 4954 1353 Size: 1446 93 471 95 1496 3423 Fragments arranged by size: 3423 1496 1446 471 95 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2240 3352 5611 5985 6131 387 Size: 1064 138 651 1112 2259 374 146 1280 Fragments arranged by size: 2259 1280 1112 1064 651 374 146 138 EcoICRI GAG'CTC Cuts at: 3949 3949 Size: 7024 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2553 2813 3561 3613 3832 5087 5684 Size: 393 921 260 748 52 219 1255 597 Cuts at: 5684 6471 6739 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1255 921 787 748 597 393 268 260 219 52 EcoO109I rG'GnC_Cy Cuts at: 3136 3737 3738 4114 3136 Size: 601 1 376 6046 Fragments arranged by size: 6046 601 376 1 EcoRI G'AATT_C Cuts at: 4333 4711 4333 Size: 378 6646 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2814 2814 Size: 7024 FspI TGC'GCA Cuts at: 4285 5530 4285 Size: 1245 5779 FspAI rTGC'GCAy Cuts at: 4285 4285 Size: 7024 HaeII r_GCGC'y Cuts at: 1678 6171 1678 Size: 4493 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2800 2832 3169 3201 3276 Size: 32 135 32 1228 32 337 32 75 Cuts at: 3276 3308 3598 3630 5665 5697 5739 5771 1373 Size: 32 290 32 2035 32 42 32 2626 Fragments arranged by size: 2626 2035 1228 337 290 135 75 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2490 4368 4614 669 Size: 988 281 552 1878 246 3079 Fragments arranged by size: 3079 1878 988 552 281 246 HindIII A'AGCT_T Cuts at: 748 3313 748 Size: 2565 4459 HpaI GTT'AAC Cuts at: 4614 4614 Size: 7024 KpnI G_GTAC'C Cuts at: 3888 4349 3888 Size: 461 6563 MfeI C'AATT_G Cuts at: 4623 4623 Size: 7024 MluI A'CGCG_T Cuts at: 6900 6900 Size: 7024 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6138 6322 1305 Size: 4833 184 2007 Fragments arranged by size: 4833 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 3112 3225 10 Size: 54 1425 343 537 743 113 3809 Fragments arranged by size: 3809 1425 743 537 343 113 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 2765 4244 4982 5341 5500 518 Size: 648 1479 120 1479 738 359 159 2042 Fragments arranged by size: 2042 1479 1479 738 648 359 159 120 MspA1I CmG'CkG Cuts at: 1606 3633 3852 3897 3925 4258 4302 4440 5117 Size: 2027 219 45 28 333 44 138 677 Cuts at: 5117 6265 6510 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 2027 1148 677 333 245 219 138 45 44 28 NaeI GCC'GGC Cuts at: 3674 3904 3674 Size: 230 6794 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5515 Fragments arranged by size: 5515 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7024 NgoMIV G'CCGG_C Cuts at: 3672 3902 3672 Size: 230 6794 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7024 NotI GC'GGCC_GC Cuts at: 4471 4471 Size: 7024 NsiI A_TGCA'T Cuts at: 2222 2222 Size: 7024 NspI r_CATG'y Cuts at: 1132 1229 2129 4382 5927 1132 Size: 97 900 2253 1545 2229 Fragments arranged by size: 2253 2229 1545 900 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7024 PciI A'CATG_T Cuts at: 5923 5923 Size: 7024 PflMI CCAn_nnn'nTGG Cuts at: 1108 4031 1108 Size: 2923 4101 PfoI T'CCnGG_A Cuts at: 2205 3458 2205 Size: 1253 5771 PmeI GTTT'AAAC Cuts at: 4328 4328 Size: 7024 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5093 5125 6606 6638 5093 Size: 32 1481 32 5479 Fragments arranged by size: 5479 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3136 4114 3136 Size: 978 6046 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7024 PsiI TTA'TAA Cuts at: 4594 4594 Size: 7024 PspOMI G'GGCC_C Cuts at: 3737 3737 Size: 7024 PstI C_TGCA'G Cuts at: 830 2608 3638 4123 4218 4376 830 Size: 1778 1030 485 95 158 3478 Fragments arranged by size: 3478 1778 1030 485 158 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7024 PvuII CAG'CTG Cuts at: 3633 3925 4302 3633 Size: 292 377 6355 Fragments arranged by size: 6355 377 292 SacI G_AGCT'C Cuts at: 3951 3951 Size: 7024 SalI G'TCGA_C Cuts at: 1936 4366 1936 Size: 2430 4594 SbfI CC_TGCA'GG Cuts at: 4376 4376 Size: 7024 ScaI AGT'ACT Cuts at: 5272 6984 5272 Size: 1712 5312 SfcI C'TryA_G Cuts at: 826 1045 2604 2771 3634 4119 4214 4372 5507 Size: 219 1559 167 863 485 95 158 1135 Cuts at: 5507 6188 6379 826 Size: 681 191 1471 Fragments arranged by size: 1559 1471 1135 863 681 485 219 191 167 158 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7024 SmaI CCC'GGG Cuts at: 1872 4351 1872 Size: 2479 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3140 5128 6029 6291 820 Size: 197 926 1197 1988 901 262 1553 Fragments arranged by size: 1988 1553 1197 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4321 4815 493 Size: 3828 494 2702 Fragments arranged by size: 3828 2702 494 SpeI A'CTAG_T Cuts at: 152 2227 152 Size: 2075 4949 SphI G_CATG'C Cuts at: 1132 4382 1132 Size: 3250 3774 SspI AAT'ATT Cuts at: 5 52 4948 5 Size: 47 4896 2081 Fragments arranged by size: 4896 2081 47 StuI AGG'CCT Cuts at: 1517 3240 1517 Size: 1723 5301 StyI C'CwwG_G Cuts at: 513 1065 2022 2085 2822 4463 513 Size: 552 957 63 737 1641 3074 Fragments arranged by size: 3074 1641 957 737 552 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2288 5051 5068 5221 5406 5851 325 Size: 1255 708 2763 17 153 185 445 1498 Fragments arranged by size: 2763 1498 1255 708 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4011 5270 6982 Size: 274 80 33 51 1443 2034 1259 1712 Cuts at: 6982 96 Size: 138 Fragments arranged by size: 2034 1712 1443 1259 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3145 3177 6984 7016 3145 Size: 32 3807 32 3153 Fragments arranged by size: 3807 3153 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3212 1316 Size: 1896 5128 XbaI T'CTAG_A Cuts at: 2296 4360 2296 Size: 2064 4960 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3796 4171 3796 Size: 375 6649 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7024 XmaI C'CCGG_G Cuts at: 1870 4349 1870 Size: 2479 4545 XmnI GAAnn'nnTTC Cuts at: 2794 5153 2794 Size: 2359 4665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4832 276 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI