Restriction map for
pFN21ASDA0093
(Linear) (Six-base) MAPSORT of: KIAA0093.seq Check: 9988 from: 1 to: 2700
KIAA0093.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1221 2700
Size: 1221 1479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1726 2455 2641 2700
Size: 1726 729 186 59
Fragments arranged by size:
1726 729 186 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2264 2700
Size: 2264 436
AlwNI CAG_nnn'CTG
Cuts at: 0 538 691 2700
Size: 538 153 2009
Fragments arranged by size:
2009 538 153
ApoI r'AATT_y
Cuts at: 0 48 927 1364 1624 2297 2700
Size: 48 879 437 260 673 403
Fragments arranged by size:
879 673 437 403 260 48
AvaI C'yCGr_G
Cuts at: 0 2492 2700
Size: 2492 208
AvrII C'CTAG_G
Cuts at: 0 306 2700
Size: 306 2394
BanI G'GyrC_C
Cuts at: 0 2496 2700
Size: 2496 204
BanII G_rGCy'C
Cuts at: 0 32 958 2592 2700
Size: 32 926 1634 108
Fragments arranged by size:
1634 926 108 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 776 1667 2700
Size: 776 891 1033
Fragments arranged by size:
1033 891 776
BbvCI CC'TCA_GC
Cuts at: 0 245 494 2700
Size: 245 249 2206
Fragments arranged by size:
2206 249 245
Bme1580I G_kGCm'C
Cuts at: 0 1550 2700
Size: 1550 1150
BmrI ACTGGGnnnn_n'
Cuts at: 0 804 2347 2700
Size: 804 1543 353
Fragments arranged by size:
1543 804 353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 566 1412 1539 2382 2700
Size: 55 511 846 127 843 318
Fragments arranged by size:
846 843 511 318 127 55
Bpu10I CC'TnA_GC
Cuts at: 0 245 494 2700
Size: 245 249 2206
Fragments arranged by size:
2206 249 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1158 2700
Size: 1158 1542
BsaI GGTCTCn'nnnn_
Cuts at: 0 357 1150 2700
Size: 357 793 1550
Fragments arranged by size:
1550 793 357
BsaAI yAC'GTr
Cuts at: 0 129 2700
Size: 129 2571
BsaWI w'CCGG_w
Cuts at: 0 1670 2700
Size: 1670 1030
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 563 1772 2700
Size: 563 1209 928
Fragments arranged by size:
1209 928 563
BseYI C'CCAG_C
Cuts at: 0 512 1238 1277 2700
Size: 512 726 39 1423
Fragments arranged by size:
1423 726 512 39
BsiHKAI G_wGCw'C
Cuts at: 0 958 2592 2700
Size: 958 1634 108
Fragments arranged by size:
1634 958 108
BsmI GAATG_Cn'
Cuts at: 0 909 2700
Size: 909 1791
Bsp1286I G_dGCh'C
Cuts at: 0 32 958 1550 2592 2700
Size: 32 926 592 1042 108
Fragments arranged by size:
1042 926 592 108 32
BspHI T'CATG_A
Cuts at: 0 2013 2700
Size: 2013 687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1221 2700
Size: 1221 1479
BsrFI r'CCGG_y
Cuts at: 0 254 2700
Size: 254 2446
BsrGI T'GTAC_A
Cuts at: 0 1140 2700
Size: 1140 1560
BstAPI GCAn_nnn'nTGC
Cuts at: 0 724 2700
Size: 724 1976
BstEII G'GTnAC_C
Cuts at: 0 1233 2700
Size: 1233 1467
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1058 2700
Size: 1058 1642
BstYI r'GATC_y
Cuts at: 0 1350 1506 2286 2700
Size: 1350 156 780 414
Fragments arranged by size:
1350 780 414 156
Bsu36I CC'TnA_GG
Cuts at: 0 671 2092 2700
Size: 671 1421 608
Fragments arranged by size:
1421 671 608
BtsI GCAGTG_nn'
Cuts at: 0 1550 2700
Size: 1550 1150
DraI TTT'AAA
Cuts at: 0 2256 2700
Size: 2256 444
DraIII CAC_nnn'GTG
Cuts at: 0 724 2700
Size: 724 1976
EarI CTCTTCn'nnn_
Cuts at: 0 587 969 1433 2700
Size: 587 382 464 1267
Fragments arranged by size:
1267 587 464 382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1960 2700
Size: 1960 740
EcoICRI GAG'CTC
Cuts at: 0 956 2590 2700
Size: 956 1634 110
Fragments arranged by size:
1634 956 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 566 1412 1539 1726 2382 2455 2641
Size: 55 511 846 127 187 656 73 186
Cuts at: 2641 2700
Size: 59
Fragments arranged by size:
846 656 511 187 186 127 73 59
55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1554 2700
Size: 1554 1146
EcoO109I rG'GnC_Cy
Cuts at: 0 617 1192 1417 2700
Size: 617 575 225 1283
Fragments arranged by size:
1283 617 575 225
EcoRV GAT'ATC
Cuts at: 0 606 749 2700
Size: 606 143 1951
Fragments arranged by size:
1951 606 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1282 2700
Size: 1282 1418
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 189 221 333 365 945 977 1879 1911
Size: 189 32 112 32 580 32 902 32
Cuts at: 1911 2008 2040 2700
Size: 97 32 660
Fragments arranged by size:
902 660 580 189 112 97 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 771 2700
Size: 771 1929
MfeI C'AATT_G
Cuts at: 0 2666 2700
Size: 2666 34
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1496 1934 2700
Size: 904 592 438 766
Fragments arranged by size:
904 766 592 438
MslI CAynn'nnrTG
Cuts at: 0 1454 2135 2700
Size: 1454 681 565
Fragments arranged by size:
1454 681 565
MspA1I CmG'CkG
Cuts at: 0 1167 2700
Size: 1167 1533
NspI r_CATG'y
Cuts at: 0 1294 2700
Size: 1294 1406
PasI CC'CwG_GG
Cuts at: 0 2679 2700
Size: 2679 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 157 1343 1772 1870 2700
Size: 157 1186 429 98 830
Fragments arranged by size:
1186 830 429 157 98
PfoI T'CCnGG_A
Cuts at: 0 32 1428 2700
Size: 32 1396 1272
Fragments arranged by size:
1396 1272 32
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1637 1669 2700
Size: 1637 32 1031
Fragments arranged by size:
1637 1031 32
PpuMI rG'GwC_Cy
Cuts at: 0 617 2700
Size: 617 2083
PshAI GACnn'nnGTC
Cuts at: 0 2541 2700
Size: 2541 159
PsiI TTA'TAA
Cuts at: 0 805 2700
Size: 805 1895
PstI C_TGCA'G
Cuts at: 0 1191 2700
Size: 1191 1509
PvuII CAG'CTG
Cuts at: 0 1167 2700
Size: 1167 1533
SacI G_AGCT'C
Cuts at: 0 958 2592 2700
Size: 958 1634 108
Fragments arranged by size:
1634 958 108
SexAI A'CCwGG_T
Cuts at: 0 1053 2700
Size: 1053 1647
SfcI C'TryA_G
Cuts at: 0 1187 1852 2391 2700
Size: 1187 665 539 309
Fragments arranged by size:
1187 665 539 309
SmlI C'TyrA_G
Cuts at: 0 1173 2700
Size: 1173 1527
SphI G_CATG'C
Cuts at: 0 1294 2700
Size: 1294 1406
SspI AAT'ATT
Cuts at: 0 227 1828 2041 2700
Size: 227 1601 213 659
Fragments arranged by size:
1601 659 227 213
StuI AGG'CCT
Cuts at: 0 89 841 1308 2700
Size: 89 752 467 1392
Fragments arranged by size:
1392 752 467 89
StyI C'CwwG_G
Cuts at: 0 306 609 2700
Size: 306 303 2091
Fragments arranged by size:
2091 306 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1672 2692 2700
Size: 1672 1020 8
Fragments arranged by size:
1672 1020 8
TatI w'GTAC_w
Cuts at: 0 1140 2700
Size: 1140 1560
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1529 1561 2700
Size: 1529 32 1139
Fragments arranged by size:
1529 1139 32
XbaI T'CTAG_A
Cuts at: 0 640 2700
Size: 640 2060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2686 2700
Size: 2686 14
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApoI AvaI AvrII BanI
BanII BbsI BbvCI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrFI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtsI DraI DraIII EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI Hin4I HincII MfeI MmeI
MslI MspA1I NspI PasI PflMI PfoI PpiI PpuMI
PshAI PsiI PstI PvuII SacI SexAI SfcI SmlI
SphI SspI StuI StyI TaqII TatI TstI XbaI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BsaBI BsaHI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BssHII
BssSI BstBI BstZ17I BtgI BtgZI ClaI CspCI DrdI
EaeI EagI EcoRI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI
XmaI XmnI ZraI