Restriction map for
pFN21ASDA0091
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbsI BcgI BfrI BlnI
BpuAI BsaBI BsiWI Bsp1407I BspDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HpaI
KpnI KspI MamI MluI NaeI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 244 GTAGAC GT^[AC][GT]AC
AccIII 2129 TCCGGA T^CCGGA
AhdI 1473 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 533 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1473 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2046 CACGTG CAC^GTG
BglI 1593 GCCTGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1467 AGATCT A^GATCT
BlpI 660 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 660 GCTGAGC GC^T[ACGT]AGC
BsaAI 2046 CACGTG [CT]AC^GT[GA]
BsaI 2750 GAGACC GGTCTC(1/5)
BseAI 2129 TCCGGA T^CCGGA
BsmBI 2818 GAGACG CGTCTC(1/5)
BspEI 2129 TCCGGA T^CCGGA
BspHI 1748 TCATGA T^CATGA
BsrBI 2937 GAGCGG CCGCTC(-3/-3)
BssSI 31 CTCGTG CACGAG(-5/-1)
BstAPI 3114 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 660 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1473 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2868 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 136 GAATTC G^AATTC
HindIII 4 AAGCTT A^AGCTT
MroI 2129 TCCGGA T^CCGGA
MspA1I 2501 CAGCTG C[AC]G^C[GT]G
NruI 2378 TCGCGA TCG^CGA
PmaCI 2046 CACGTG CAC^GTG
PmlI 2046 CACGTG CAC^GTG
PpuMI 2027 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 589 CTGCAG CTGCA^G
PvuI 2896 CGATCG CGAT^CG
PvuII 2501 CAGCTG CAG^CTG
RcaI 1748 TCATGA T^CATGA
ScaI 2102 AGTACT AGT^ACT
SfcI 589 CTGCAG C^T[GA][CT]AG
SmaI 1360 CCCGGG CCC^GGG
SphI 522 GCATGC GCATG^C
XmaCI 1360 CCCGGG C^CCGGG
XmaI 1360 CCCGGG C^CCGGG
XmnI 533 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcsI 136 GAATTC [GA]^AATT[CT]
207 AAATTC
ApaI 1223 GGGCCC GGGCC^C
2036 GGGCCC
ApoI 136 GAATTC [GA]^AATT[CT]
207 AAATTC
AspHI 601 GTGCTC G[AT]GC[AT]^C
1284 GAGCAC
AspI 1168 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1776 GACAGAGTC
AvaI 1360 CCCGGG C^[CT]CG[GA]G
2032 CTCGGG
BalI 1685 TGGCCA TGG^CCA
2339 TGGCCA
BbeI 2823 GGCGCC GGCGC^C
2941 GGCGCC
BbvCI 915 CCTCAGC CCTCAGC(-5/-2)
2503 GCTGAGG
BciVI 184 GGATAC GTATCC(6/5)
2244 GGATAC
BsaWI 2129 TCCGGA [AT]^CCGG[AT]
2248 ACCGGA
BsgI 1793 GTGCAG GTGCAG(16/14)
2370 GTGCAG
BsiEI 1152 CGGTCG CG[GA][CT]^CG
2896 CGATCG
BsiHKAI 601 GTGCTC G[AT]GC[AT]^C
1284 GAGCAC
BsmI 1526 GAATGC GAATGC(1/-1)
537 GCATTC
BsoBI 1360 CCCGGG C^[CT]CG[GA]G
2032 CTCGGG
BstYI 1418 AGATCC [GA]^GATC[CT]
1467 AGATCT
KasI 2823 GGCGCC G^GCGCC
2941 GGCGCC
MfeI 249 CAATTG C^AATTG
2541 CAATTG
MflI 1418 AGATCC [GA]^GATC[CT]
1467 AGATCT
MluNI 1685 TGGCCA TGG^CCA
2339 TGGCCA
MscI 1685 TGGCCA TGG^CCA
2339 TGGCCA
MunI 249 CAATTG C^AATTG
2541 CAATTG
NarI 2823 GGCGCC GG^CGCC
2941 GGCGCC
NcoI 744 CCATGG C^CATGG
3029 CCATGG
PflFI 1168 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1776 GACAGAGTC
SapI 231 GAAGAGC GCTCTTC(1/4)
488 GAAGAGC
SfoI 2823 GGCGCC GGC^GCC
2941 GGCGCC
SmlI 406 CTCAAG C^T[CT][GA]AG
2909 CTCAAG
Tth111I 1168 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1776 GACAGAGTC
XhoII 1418 AGATCC [GA]^GATC[CT]
1467 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaII BanI
BanII BbvI BclI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI