(Circular) (Six-base) MAPSORT of: pFN21ASDA0091.seq Check: 9878 from: 1 to: 7885 pFN21ASDA0091 7885 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2774 3275 2774 Size: 501 7384 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5695 278 Size: 53 83 186 936 4159 2468 Fragments arranged by size: 4159 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2268 5228 1937 Size: 331 2960 4594 Fragments arranged by size: 4594 2960 331 Acc65I G'GTAC_C Cuts at: 5206 5206 Size: 7885 AclI AA'CG_TT Cuts at: 1563 6012 6385 1563 Size: 4449 373 3063 Fragments arranged by size: 4449 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 4155 4174 5948 7332 7600 1632 Size: 641 1882 19 1774 1384 268 1917 Fragments arranged by size: 1917 1882 1774 1384 641 268 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7688 AflIII A'CryG_T Cuts at: 1203 2928 3393 3510 4070 4374 6784 7761 1203 Size: 1725 465 117 560 304 2410 977 1327 Fragments arranged by size: 2410 1725 1327 977 560 465 304 117 AgeI A'CCGG_T Cuts at: 6716 6716 Size: 7885 AhdI GACnn_n'nnGTC Cuts at: 3501 6614 3501 Size: 3113 4772 AleI CACnn'nnGTG Cuts at: 1166 2777 1166 Size: 1611 6274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2591 1184 Size: 1407 6478 AlwNI CAG_nnn'CTG Cuts at: 2611 3290 3358 4024 4197 5628 7200 2611 Size: 679 68 666 173 1431 1572 3296 Fragments arranged by size: 3296 1572 1431 679 666 173 68 ApaI G_GGCC'C Cuts at: 3250 4063 3250 Size: 813 7072 ApaLI G'TGCA_C Cuts at: 5942 7098 5942 Size: 1156 6729 ApoI r'AATT_y Cuts at: 1493 2159 2230 5194 5420 5572 7681 1493 Size: 666 71 2964 226 152 2109 1697 Fragments arranged by size: 2964 2109 1697 666 226 152 71 AseI AT'TA_AT Cuts at: 160 1032 6439 160 Size: 872 5407 1606 Fragments arranged by size: 5407 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7885 AvaI C'yCGr_G Cuts at: 1870 1943 3383 4055 5210 1870 Size: 73 1440 672 1155 4545 Fragments arranged by size: 4545 1440 1155 672 73 BamHI G'GATC_C Cuts at: 5215 5582 5215 Size: 367 7518 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3240 4846 4964 5206 6661 Size: 325 217 627 1453 1606 118 242 1455 Cuts at: 6661 618 Size: 1842 Fragments arranged by size: 1842 1606 1455 1453 627 325 242 217 118 BanII G_rGCy'C Cuts at: 1391 2534 3250 3479 4063 5204 1391 Size: 1143 716 229 584 1141 4072 Fragments arranged by size: 4072 1143 1141 716 584 229 BbeI G_GCGC'C Cuts at: 4850 4968 4850 Size: 118 7767 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6914 BbvCI CC'TCA_GC Cuts at: 2939 4527 2939 Size: 1588 6297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6076 6110 1903 Size: 34 4139 34 3678 Fragments arranged by size: 4139 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2201 4261 5777 6987 2201 Size: 2060 1516 1210 3099 Fragments arranged by size: 3099 2060 1516 1210 BclI T'GATC_A Cuts at: 1548 3073 3774 3985 1548 Size: 1525 701 211 5448 Fragments arranged by size: 5448 1525 701 211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3622 6496 136 Size: 107 122 71 631 605 1950 2874 1525 Fragments arranged by size: 2874 1950 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3490 7880 3490 Size: 4390 3495 BlpI GC'TnA_GC Cuts at: 2684 5302 2684 Size: 2618 5267 Bme1580I G_kGCm'C Cuts at: 1790 2251 3250 4063 5946 7102 1790 Size: 461 999 813 1883 1156 2573 Fragments arranged by size: 2573 1883 1156 999 813 461 BmgBI CAC'GTC Cuts at: 1318 2501 2929 4162 1318 Size: 1183 428 1233 5041 Fragments arranged by size: 5041 1233 1183 428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2688 4683 4845 Size: 416 53 83 399 24 1271 1995 162 Cuts at: 4845 5616 6574 442 Size: 771 958 1753 Fragments arranged by size: 1995 1753 1271 958 771 416 399 162 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7885 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2537 2537 Size: 7885 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2808 3258 4607 4695 4778 4974 5001 6545 Size: 1569 450 1349 88 83 196 27 1544 Cuts at: 6545 1239 Size: 2579 Fragments arranged by size: 2579 1569 1544 1349 450 196 88 83 27 Bpu10I CC'TnA_GC Cuts at: 2939 3759 4527 2939 Size: 820 768 6297 Fragments arranged by size: 6297 820 768 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 4917 6010 6875 7173 2414 Size: 2503 1093 865 298 3126 Fragments arranged by size: 3126 2503 1093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4767 6548 882 Size: 617 3268 1781 2219 Fragments arranged by size: 3268 2219 1781 617 BsaAI yAC'GTr Cuts at: 493 1204 4071 5182 5676 493 Size: 711 2867 1111 494 2702 Fragments arranged by size: 2867 2702 1111 711 494 BsaBI GATnn'nnATC Cuts at: 7879 7879 Size: 7885 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2895 4422 4847 4965 Size: 53 83 186 936 1362 1527 425 118 Cuts at: 4965 5692 6074 6709 275 Size: 727 382 635 1451 Fragments arranged by size: 1527 1451 1362 936 727 635 425 382 186 118 83 53 BsaWI w'CCGG_w Cuts at: 4152 4271 6318 6716 6990 7137 7548 4152 Size: 119 2047 398 274 147 411 4489 Fragments arranged by size: 4489 2047 411 398 274 147 119 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5040 5070 1178 Size: 30 165 30 3637 30 3993 Fragments arranged by size: 3993 3637 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3667 4133 4136 4290 4610 1186 Size: 2481 466 3 154 320 4461 Fragments arranged by size: 4461 2481 466 320 154 3 BseYI C'CCAG_C Cuts at: 3479 3528 3790 4288 7088 7584 3479 Size: 49 262 498 2800 496 3780 Fragments arranged by size: 3780 2800 498 496 262 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3837 4414 1163 Size: 573 2101 577 4634 Fragments arranged by size: 4634 2101 577 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 4922 5335 6096 7124 1540 Size: 275 186 1177 1744 413 761 1028 2301 Fragments arranged by size: 2301 1744 1177 1028 761 413 275 186 BsiHKAI G_wGCw'C Cuts at: 2628 3311 5946 6031 7102 2628 Size: 683 2635 85 1071 3411 Fragments arranged by size: 3411 2635 1071 683 85 BsmI GAATG_Cn' Cuts at: 2560 3555 5396 5489 2560 Size: 995 1841 93 4956 Fragments arranged by size: 4956 1841 995 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4835 4835 Size: 7885 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2251 2534 2628 3250 3311 3479 4063 Size: 399 461 283 94 622 61 168 584 Cuts at: 4063 5204 5946 6031 7102 1391 Size: 1141 742 85 1071 2174 Fragments arranged by size: 2174 1141 1071 742 622 584 461 399 283 168 94 85 61 BspEI T'CCGG_A Cuts at: 4152 7548 4152 Size: 3396 4489 BspHI T'CATG_A Cuts at: 3771 5774 7504 3771 Size: 2003 1730 4152 Fragments arranged by size: 4152 2003 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2774 3275 5140 5240 844 Size: 1930 501 1865 100 3489 Fragments arranged by size: 3489 1930 1865 501 100 BsrBI CCG'CTC Cuts at: 1394 1823 4962 5772 1394 Size: 429 3139 810 3507 Fragments arranged by size: 3507 3139 810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2470 3040 3753 6380 6554 65 Size: 1183 1222 570 713 2627 174 1396 Fragments arranged by size: 2627 1396 1222 1183 713 570 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6529 6716 1700 Size: 110 4719 187 2869 Fragments arranged by size: 4719 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7885 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7885 BssSI C'ACGA_G Cuts at: 2054 5945 6957 2054 Size: 3891 1012 2982 Fragments arranged by size: 3891 2982 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5143 794 Size: 4349 3536 BstBI TT'CG_AA Cuts at: 2007 6689 2007 Size: 4682 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7885 BstXI CCAn_nnnn'nTGG Cuts at: 4570 4756 4891 4570 Size: 186 135 7564 Fragments arranged by size: 7564 186 135 BstYI r'GATC_y Cuts at: 1972 3441 3490 5215 5582 5967 5984 7436 7522 Size: 1469 49 1725 367 385 17 1452 86 Cuts at: 7522 7534 7880 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1725 1469 1452 385 367 346 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2767 3409 5052 513 Size: 552 1702 642 1643 3346 Fragments arranged by size: 3346 1702 1643 642 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3106 4347 4384 487 Size: 683 56 1880 1241 37 3988 Fragments arranged by size: 3988 1880 1241 683 56 37 BtsI GCAGTG_nn' Cuts at: 2266 2609 4259 5401 6195 6215 2266 Size: 343 1650 1142 794 20 3936 Fragments arranged by size: 3936 1650 1142 794 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7885 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2157 2192 529 Size: 35 1593 35 6222 Fragments arranged by size: 6222 1593 35 35 DraI TTT'AAA Cuts at: 2916 4467 4836 5189 2916 Size: 1551 369 353 5612 Fragments arranged by size: 5612 1551 369 353 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6892 809 Size: 507 5576 1802 Fragments arranged by size: 5576 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3708 4362 4996 Size: 54 1425 50 275 18 1878 654 634 Cuts at: 4996 5332 6221 7850 8 Size: 336 889 1629 43 Fragments arranged by size: 1878 1629 1425 889 654 634 336 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5332 1537 Size: 275 3520 4090 Fragments arranged by size: 4090 3520 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2506 4131 5815 1353 Size: 896 257 1625 1684 3423 Fragments arranged by size: 3423 1684 1625 896 257 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6472 6846 6992 387 Size: 1064 138 4883 374 146 1280 Fragments arranged by size: 4883 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2808 3258 4155 4174 4607 4695 Size: 393 641 535 450 897 19 433 88 Cuts at: 4695 4778 4974 5001 5948 6545 7332 7600 1239 Size: 83 196 27 947 597 787 268 1524 Fragments arranged by size: 1524 947 897 787 641 597 535 450 433 393 268 196 88 83 27 19 EcoNI CCTnn'n_nnAGG Cuts at: 4895 4895 Size: 7885 EcoO109I rG'GnC_Cy Cuts at: 2119 3247 3355 4051 4059 4060 4941 2119 Size: 1128 108 696 8 1 881 5063 Fragments arranged by size: 5063 1128 881 696 108 8 1 EcoRI G'AATT_C Cuts at: 2159 5194 5572 2159 Size: 3035 378 4472 Fragments arranged by size: 4472 3035 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4615 4615 Size: 7885 FspI TGC'GCA Cuts at: 6391 6391 Size: 7885 HaeII r_GCGC'y Cuts at: 1678 4684 4850 4968 5134 7032 1678 Size: 3006 166 118 166 1898 2531 Fragments arranged by size: 3006 2531 1898 166 166 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3487 3519 3788 3820 4269 Size: 32 135 32 1915 32 269 32 449 Cuts at: 4269 4292 4301 4324 6526 6558 6600 6632 1373 Size: 23 9 23 2202 32 42 32 2626 Fragments arranged by size: 2626 2202 1915 449 269 135 42 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 2035 2980 3391 5229 5475 669 Size: 988 281 97 945 411 1838 246 3079 Fragments arranged by size: 3079 1838 988 945 411 281 246 97 HindIII A'AGCT_T Cuts at: 748 2027 748 Size: 1279 6606 HpaI GTT'AAC Cuts at: 5475 5475 Size: 7885 KasI G'GCGC_C Cuts at: 4846 4964 4846 Size: 118 7767 KpnI G_GTAC'C Cuts at: 5210 5210 Size: 7885 MfeI C'AATT_G Cuts at: 2272 4564 5484 2272 Size: 2292 920 4673 Fragments arranged by size: 4673 2292 920 MluI A'CGCG_T Cuts at: 7761 7761 Size: 7885 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6999 7183 1305 Size: 5694 184 2007 Fragments arranged by size: 5694 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3710 4364 10 Size: 54 1425 343 1878 654 3531 Fragments arranged by size: 3531 1878 1425 654 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2777 3722 3851 5843 6202 6361 518 Size: 648 1611 945 129 1992 359 159 2042 Fragments arranged by size: 2042 1992 1611 945 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 4526 5301 5978 7126 7371 1606 Size: 2920 775 677 1148 245 2120 Fragments arranged by size: 2920 2120 1148 775 677 245 NarI GG'CG_CC Cuts at: 4847 4965 4847 Size: 118 7767 NcoI C'CATG_G Cuts at: 513 1065 2767 5052 513 Size: 552 1702 2285 3346 Fragments arranged by size: 3346 2285 1702 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7885 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7885 NotI GC'GGCC_GC Cuts at: 5332 5332 Size: 7885 NruI TCG'CGA Cuts at: 4403 4403 Size: 7885 NspI r_CATG'y Cuts at: 1132 1229 2549 3397 3514 4378 5243 6788 1132 Size: 97 1320 848 117 864 865 1545 2229 Fragments arranged by size: 2229 1545 1320 865 864 848 117 97 PasI CC'CwG_GG Cuts at: 1348 2527 3939 5121 1348 Size: 1179 1412 1182 4112 Fragments arranged by size: 4112 1412 1182 1179 PciI A'CATG_T Cuts at: 3393 3510 4374 6784 3393 Size: 117 864 2410 4494 Fragments arranged by size: 4494 2410 864 117 PflMI CCAn_nnn'nTGG Cuts at: 1108 2129 4418 4456 5014 1108 Size: 1021 2289 38 558 3979 Fragments arranged by size: 3979 2289 1021 558 38 PfoI T'CCnGG_A Cuts at: 4978 4978 Size: 7885 PmeI GTTT'AAAC Cuts at: 5189 5189 Size: 7885 PmlI CAC'GTG Cuts at: 4071 4071 Size: 7885 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5954 5986 7467 7499 5954 Size: 32 1481 32 6340 Fragments arranged by size: 6340 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4051 4051 Size: 7885 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7885 PsiI TTA'TAA Cuts at: 5455 5455 Size: 7885 PspOMI G'GGCC_C Cuts at: 3246 4059 3246 Size: 813 7072 PstI C_TGCA'G Cuts at: 830 2616 5237 830 Size: 1786 2621 3478 Fragments arranged by size: 3478 2621 1786 PvuI CG_AT'CG Cuts at: 2001 4922 2001 Size: 2921 4964 PvuII CAG'CTG Cuts at: 4526 4526 Size: 7885 SalI G'TCGA_C Cuts at: 1936 5227 1936 Size: 3291 4594 SapI GCTCTTCn'nnn_ Cuts at: 2249 2506 2249 Size: 257 7628 SbfI CC_TGCA'GG Cuts at: 5237 5237 Size: 7885 ScaI AGT'ACT Cuts at: 4127 6133 7845 4127 Size: 2006 1712 4167 Fragments arranged by size: 4167 2006 1712 SfcI C'TryA_G Cuts at: 826 1045 2612 5233 6368 7049 7240 826 Size: 219 1567 2621 1135 681 191 1471 Fragments arranged by size: 2621 1567 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 4848 4966 4848 Size: 118 7767 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7885 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7885 SmaI CCC'GGG Cuts at: 1872 3385 5212 1872 Size: 1513 1827 4545 Fragments arranged by size: 4545 1827 1513 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 4932 5989 6890 7152 820 Size: 197 926 486 2503 1057 901 262 1553 Fragments arranged by size: 2503 1553 1057 926 901 486 262 197 SnaBI TAC'GTA Cuts at: 493 5182 5676 493 Size: 4689 494 2702 Fragments arranged by size: 4689 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7885 SphI G_CATG'C Cuts at: 1132 2549 5243 1132 Size: 1417 2694 3774 Fragments arranged by size: 3774 2694 1417 SspI AAT'ATT Cuts at: 5 52 5809 5 Size: 47 5757 2081 Fragments arranged by size: 5757 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7885 StyI C'CwwG_G Cuts at: 513 1065 2767 2812 3692 4450 4749 5052 5324 Size: 552 1702 45 880 758 299 303 272 Cuts at: 5324 513 Size: 3074 Fragments arranged by size: 3074 1702 880 758 552 303 299 272 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3670 5912 5929 6082 6267 6712 325 Size: 1255 2090 2242 17 153 185 445 1498 Fragments arranged by size: 2242 2090 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4125 4546 4626 Size: 274 80 33 51 1443 2148 421 80 Cuts at: 4626 6131 7843 96 Size: 1505 1712 138 Fragments arranged by size: 2148 1712 1505 1443 421 274 138 80 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5040 5072 7845 7877 5040 Size: 32 2773 32 5048 Fragments arranged by size: 5048 2773 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3194 3802 1316 Size: 1878 608 5399 Fragments arranged by size: 5399 1878 608 XbaI T'CTAG_A Cuts at: 5221 5221 Size: 7885 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2524 4812 5059 2524 Size: 2288 247 5350 Fragments arranged by size: 5350 2288 247 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7885 XmaI C'CCGG_G Cuts at: 1870 3383 5210 1870 Size: 1513 1827 4545 Fragments arranged by size: 4545 1827 1513 XmnI GAAnn'nnTTC Cuts at: 2560 6014 2560 Size: 3454 4431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5693 276 Size: 53 83 186 936 4159 2468 Fragments arranged by size: 4159 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI