Restriction map for
pFN21ASDA0088
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# NO CUT ENZYMES ( 91 enzymes) #
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AccIII AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
AspEI AspI BbeI BbrPI BcgI
BfrI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI HindIII KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 796 GACGTC GACGT^C
Acc65I 1697 GGTACC G^GTACC
AccI 1108 GTAGAC GT^[AC][GT]AC
AccII 634 CGCG CG^CG
AcyI 796 GACGTC G[GA]^CG[CT]C
Alw44I 1444 GTGCAC G^TGCAC
ApaLI 1444 GTGCAC G^TGCAC
Asp700I 1870 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1697 GGTACC G^GTACC
AviII 2492 TGCGCA TGC^GCA
AvrII 259 CCTAGG C^CTAGG
BamHI 482 GGATCC G^GATCC
BanI 1697 GGTACC G^G[CT][GA]CC
BciVI 2105 GTATCC GTATCC(6/5)
BglI 1264 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1589 AGATCT A^GATCT
BlnI 259 CCTAGG C^CTAGG
BsaHI 796 GACGTC G[GA]^CG[CT]C
BsgI 1900 GTGCAG GTGCAG(16/14)
BsmI 1874 GCATTC GAATGC(1/-1)
Bsp1407I 575 TGTACA T^GTACA
BspHI 1883 TCATGA T^CATGA
BspLU11I 1277 ACATGT A^CATGT
BsrBI 2377 GAGCGG CCGCTC(-3/-3)
BsrGI 575 TGTACA T^GTACA
BssSI 1147 CACGAG CACGAG(-5/-1)
BstUI 634 CGCG CG^CG
EarI 880 CTCTTC CTCTTC(1/4)
FspI 2492 TGCGCA TGC^GCA
HgaI 2295 GCGTC GACGC(5/10)
Hin1I 796 GACGTC G[GA]^CG[CT]C
HincII 126 GTTAAC GT[CT]^[GA]AC
HindII 126 GTTAAC GT[CT]^[GA]AC
HpaI 126 GTTAAC GTT^AAC
KpnI 1697 GGTACC GGTAC^C
Ksp632I 880 CTCTTC CTCTTC(1/4)
MvnI 634 CGCG CG^CG
NruI 633 TCGCGA TCG^CGA
RcaI 1883 TCATGA T^CATGA
SacI 2236 GAGCTC GAGCT^C
SbfI 732 CCTGCAGG CCTGCA^GG
ScaI 1943 AGTACT AGT^ACT
SmaI 1184 CCCGGG CCC^GGG
SphI 492 GCATGC GCATG^C
Sse8387I 732 CCTGCAGG CCTGCA^GG
SspBI 575 TGTACA T^GTACA
TfiI 2300 GATTC G^A[AT]TC
XmaCI 1184 CCCGGG C^CCGGG
XmaI 1184 CCCGGG C^CCGGG
XmnI 1870 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 1277 ACATGT A^C[GA][CT]GT
2258 ACACGT
AvaI 224 CCCGAG C^[CT]CG[GA]G
1184 CCCGGG
BclI 960 TGATCA T^GATCA
1938 TGATCA
BsoBI 224 CCCGAG C^[CT]CG[GA]G
1184 CCCGGG
BstEII 1231 GGTTACC G^GT[ACGT]ACC
1368 GGTCACC
BstPI 1231 GGTTACC G^GT[ACGT]ACC
1368 GGTCACC
EcoNI 186 CCTACTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2017 CCTGGCCAAGG
EcoO65I 1231 GGTTACC G^GT[ACGT]ACC
1368 GGTCACC
FauI 1055 CCCGC CCCGC(4/6)
2379 GCGGG
FbaI 960 TGATCA T^GATCA
1938 TGATCA
MaeII 797 ACGT A^CGT
2260 ACGT
MseI 127 TTAA T^TAA
1178 TTAA
PpuMI 141 GGGACCC [GA]G^G[AT]CC[CT]
1031 GGGACCC
PshAI 196 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1033 GACCCCAGTC
PstI 662 CTGCAG CTGCA^G
733 CTGCAG
SfcI 662 CTGCAG C^T[GA][CT]AG
733 CTGCAG
TaqI 2142 TCGA T^CGA
2298 TCGA
Tru9I 127 TTAA T^TAA
1178 TTAA
Tsp509I 550 AATT ^AATT
1294 AATT
TthHB8I 2142 TCGA T^CGA
2298 TCGA
XcmI 1740 CCACTTGGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2055 CCAGAAGCATCATGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
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AciI AfaI AluI AlwI AlwNI
AocI Aor51HI ApaI AspHI AvaII
BalI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco47III Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TseI Tsp45I TspRI
XhoII