(Circular) (Six-base) MAPSORT of: pFN21ASDA0086.seq Check: 2981 from: 1 to: 7849 pFN21ASDA0086 7849 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2605 3265 3619 2605 Size: 660 354 6835 Fragments arranged by size: 6835 660 354 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5659 278 Size: 53 83 186 936 4123 2468 Fragments arranged by size: 4123 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5192 1937 Size: 3255 4594 Acc65I G'GTAC_C Cuts at: 5170 5170 Size: 7849 AclI AA'CG_TT Cuts at: 1563 4492 5976 6349 1563 Size: 2929 1484 373 3063 Fragments arranged by size: 3063 2929 1484 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2769 2884 3098 4026 4453 5063 5912 7296 Size: 1137 115 214 928 427 610 849 1384 Cuts at: 7296 7564 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1137 928 849 610 427 268 214 115 AfeI AGC'GCT Cuts at: 5027 5027 Size: 7849 AflII C'TTAA_G Cuts at: 820 1017 3647 820 Size: 197 2630 5022 Fragments arranged by size: 5022 2630 197 AflIII A'CryG_T Cuts at: 1203 2347 2349 4121 4791 6748 7725 1203 Size: 1144 2 1772 670 1957 977 1327 Fragments arranged by size: 1957 1772 1327 1144 977 670 2 AgeI A'CCGG_T Cuts at: 6680 6680 Size: 7849 AhdI GACnn_n'nnGTC Cuts at: 6578 6578 Size: 7849 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7849 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3553 1184 Size: 2369 5480 AlwNI CAG_nnn'CTG Cuts at: 2907 4004 4412 5041 5592 7164 2907 Size: 1097 408 629 551 1572 3592 Fragments arranged by size: 3592 1572 1097 629 551 408 ApaLI G'TGCA_C Cuts at: 5906 7062 5906 Size: 1156 6693 ApoI r'AATT_y Cuts at: 1493 2263 2875 3323 3854 3946 4986 5158 5384 Size: 770 612 448 531 92 1040 172 226 Cuts at: 5384 5536 7645 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1040 770 612 531 448 226 172 152 92 AseI AT'TA_AT Cuts at: 160 1032 3993 4770 4833 6403 160 Size: 872 2961 777 63 1570 1606 Fragments arranged by size: 2961 1606 1570 872 777 63 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7849 AvaI C'yCGr_G Cuts at: 1870 1943 3123 5174 1870 Size: 73 1180 2051 4545 Fragments arranged by size: 4545 2051 1180 73 BamHI G'GATC_C Cuts at: 5179 5546 5179 Size: 367 7482 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5078 5170 6625 618 Size: 325 217 627 3291 92 1455 1842 Fragments arranged by size: 3291 1842 1455 627 325 217 92 BanII G_rGCy'C Cuts at: 1391 5168 1391 Size: 3777 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2052 2489 2655 2949 4027 4672 Size: 971 138 15 437 166 294 1078 645 Cuts at: 4672 928 Size: 4105 Fragments arranged by size: 4105 1078 971 645 437 294 166 138 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2414 2448 6040 6074 1903 Size: 34 477 34 3592 34 3678 Fragments arranged by size: 3678 3592 477 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2375 5741 6951 2375 Size: 3366 1210 3273 Fragments arranged by size: 3366 3273 1210 BclI T'GATC_A Cuts at: 1548 2572 4228 1548 Size: 1024 1656 5169 Fragments arranged by size: 5169 1656 1024 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4613 6460 136 Size: 107 122 71 631 605 2941 1847 1525 Fragments arranged by size: 2941 1847 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7844 7844 Size: 7849 BlpI GC'TnA_GC Cuts at: 5266 5266 Size: 7849 Bme1580I G_kGCm'C Cuts at: 1790 2220 5081 5910 7066 1790 Size: 430 2861 829 1156 2573 Fragments arranged by size: 2861 2573 1156 829 430 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7849 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5580 6538 442 Size: 416 53 83 399 24 4163 958 1753 Fragments arranged by size: 4163 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2555 1056 Size: 1499 6350 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2556 2556 Size: 7849 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 6509 1239 Size: 1059 4211 2579 Fragments arranged by size: 4211 2579 1059 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2669 5974 6839 7137 2669 Size: 3305 865 298 3381 Fragments arranged by size: 3381 3305 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6512 882 Size: 617 5013 2219 Fragments arranged by size: 5013 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2350 5146 5640 493 Size: 711 1146 2796 494 2702 Fragments arranged by size: 2796 2702 1146 711 494 BsaBI GATnn'nnATC Cuts at: 4395 7843 4395 Size: 3448 4401 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2634 5656 6038 6673 Size: 53 83 186 936 1101 3022 382 635 Cuts at: 6673 275 Size: 1451 Fragments arranged by size: 3022 1451 1101 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2968 4587 6282 6680 6954 7101 7512 2968 Size: 1619 1695 398 274 147 411 3305 Fragments arranged by size: 3305 1695 1619 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3598 3628 1178 Size: 30 165 30 2195 30 5399 Fragments arranged by size: 5399 2195 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7849 BseYI C'CCAG_C Cuts at: 4481 7052 7548 4481 Size: 2571 496 4782 Fragments arranged by size: 4782 2571 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4816 1163 Size: 573 3080 4196 Fragments arranged by size: 4196 3080 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5299 6060 7088 1540 Size: 275 186 3298 761 1028 2301 Fragments arranged by size: 3298 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5099 5910 5995 7066 5099 Size: 811 85 1071 5882 Fragments arranged by size: 5882 1071 811 85 BsmI GAATG_Cn' Cuts at: 3511 5360 5453 3511 Size: 1849 93 5907 Fragments arranged by size: 5907 1849 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2745 2745 Size: 7849 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 5081 5099 5168 5910 5995 7066 Size: 399 430 2861 18 69 742 85 1071 Cuts at: 7066 1391 Size: 2174 Fragments arranged by size: 2861 2174 1071 742 430 399 85 69 18 BspEI T'CCGG_A Cuts at: 7512 7512 Size: 7849 BspHI T'CATG_A Cuts at: 2950 4416 5738 7468 2950 Size: 1466 1322 1730 3331 Fragments arranged by size: 3331 1730 1466 1322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2222 2605 3265 3619 5204 844 Size: 1378 383 660 354 1585 3489 Fragments arranged by size: 3489 1585 1378 660 383 354 BsrBI CCG'CTC Cuts at: 1394 1823 5736 1394 Size: 429 3913 3507 Fragments arranged by size: 3913 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2882 4337 4689 6344 6518 65 Size: 1183 1634 1455 352 1655 174 1396 Fragments arranged by size: 1655 1634 1455 1396 1183 352 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4151 6493 6680 1700 Size: 110 2341 2342 187 2869 Fragments arranged by size: 2869 2342 2341 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7849 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7849 BssSI C'ACGA_G Cuts at: 5909 6921 5909 Size: 1012 6837 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7849 BstBI TT'CG_AA Cuts at: 2007 6653 2007 Size: 4646 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7849 BstYI r'GATC_y Cuts at: 1972 4477 5179 5546 5931 5948 7400 7486 7498 Size: 2505 702 367 385 17 1452 86 12 Cuts at: 7498 7844 1972 Size: 346 1977 Fragments arranged by size: 2505 1977 1452 702 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7110 Fragments arranged by size: 7110 683 56 BtsI GCAGTG_nn' Cuts at: 3821 3950 4602 5365 6159 6179 3821 Size: 129 652 763 794 20 5491 Fragments arranged by size: 5491 794 763 652 129 20 ClaI AT'CG_AT Cuts at: 1554 2958 1554 Size: 1404 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3243 3278 529 Size: 35 2679 35 5100 Fragments arranged by size: 5100 2679 35 35 DraI TTT'AAA Cuts at: 2728 3055 3585 5153 2728 Size: 327 530 1568 5424 Fragments arranged by size: 5424 1568 530 327 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4514 6856 809 Size: 507 3198 2342 1802 Fragments arranged by size: 3198 2342 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5296 6185 7814 Size: 54 1425 50 275 18 3466 889 1629 Cuts at: 7814 8 Size: 43 Fragments arranged by size: 3466 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5296 1537 Size: 275 3484 4090 Fragments arranged by size: 4090 3484 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3399 3729 3968 4503 5779 1353 Size: 2046 330 239 535 1276 3423 Fragments arranged by size: 3423 2046 1276 535 330 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6436 6810 6956 387 Size: 1064 138 4847 374 146 1280 Fragments arranged by size: 4847 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2298 2769 2884 3098 4026 4453 5063 Size: 393 666 471 115 214 928 427 610 Cuts at: 5063 5912 6509 7296 7564 1239 Size: 849 597 787 268 1524 Fragments arranged by size: 1524 928 849 787 666 610 597 471 427 393 268 214 115 EcoNI CCTnn'n_nnAGG Cuts at: 2601 2601 Size: 7849 EcoO109I rG'GnC_Cy Cuts at: 3807 3807 Size: 7849 EcoRI G'AATT_C Cuts at: 2263 4986 5158 5536 2263 Size: 2723 172 378 4576 Fragments arranged by size: 4576 2723 378 172 EcoRV GAT'ATC Cuts at: 2956 2956 Size: 7849 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2237 2679 3410 3467 3684 2237 Size: 442 731 57 217 6402 Fragments arranged by size: 6402 731 442 217 57 FspI TGC'GCA Cuts at: 6355 6355 Size: 7849 HaeII r_GCGC'y Cuts at: 1678 5029 6996 1678 Size: 3351 1967 2531 Fragments arranged by size: 3351 2531 1967 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2612 2644 2755 2787 3967 Size: 32 135 32 1040 32 111 32 1180 Cuts at: 3967 3999 6490 6522 6564 6596 1373 Size: 32 2491 32 42 32 2626 Fragments arranged by size: 2626 2491 1180 1040 135 111 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5193 5439 669 Size: 988 281 3255 246 3079 Fragments arranged by size: 3255 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3272 4307 748 Size: 2524 1035 4290 Fragments arranged by size: 4290 2524 1035 HpaI GTT'AAC Cuts at: 5439 5439 Size: 7849 KpnI G_GTAC'C Cuts at: 5174 5174 Size: 7849 MfeI C'AATT_G Cuts at: 5448 5448 Size: 7849 MluI A'CGCG_T Cuts at: 7725 7725 Size: 7849 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6963 7147 1305 Size: 5658 184 2007 Fragments arranged by size: 5658 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6027 Fragments arranged by size: 6027 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2434 5807 6166 6325 518 Size: 648 1268 3373 359 159 2042 Fragments arranged by size: 3373 2042 1268 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3042 5265 5942 7090 7335 1606 Size: 1436 2223 677 1148 245 2120 Fragments arranged by size: 2223 2120 1436 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7297 NdeI CA'TA_TG Cuts at: 387 4521 387 Size: 4134 3715 NheI G'CTAG_C Cuts at: 1052 2551 1052 Size: 1499 6350 NotI GC'GGCC_GC Cuts at: 5296 5296 Size: 7849 NspI r_CATG'y Cuts at: 1132 1229 2444 3228 3534 4125 4637 4723 4795 Size: 97 1215 784 306 591 512 86 72 Cuts at: 4795 5207 6752 1132 Size: 412 1545 2229 Fragments arranged by size: 2229 1545 1215 784 591 512 412 306 97 86 72 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7849 PciI A'CATG_T Cuts at: 4121 4791 6748 4121 Size: 670 1957 5222 Fragments arranged by size: 5222 1957 670 PflMI CCAn_nnn'nTGG Cuts at: 1108 2099 1108 Size: 991 6858 PfoI T'CCnGG_A Cuts at: 2314 2314 Size: 7849 PmeI GTTT'AAAC Cuts at: 5153 5153 Size: 7849 PmlI CAC'GTG Cuts at: 2350 2350 Size: 7849 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5918 5950 7431 7463 5918 Size: 32 1481 32 6304 Fragments arranged by size: 6304 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3807 3807 Size: 7849 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7849 PsiI TTA'TAA Cuts at: 5419 5419 Size: 7849 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4141 4173 4141 Size: 32 7817 PstI C_TGCA'G Cuts at: 830 3542 3957 5201 830 Size: 2712 415 1244 3478 Fragments arranged by size: 3478 2712 1244 415 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7849 PvuII CAG'CTG Cuts at: 3042 3042 Size: 7849 SalI G'TCGA_C Cuts at: 1936 5191 1936 Size: 3255 4594 SbfI CC_TGCA'GG Cuts at: 5201 5201 Size: 7849 ScaI AGT'ACT Cuts at: 3205 3868 6097 7809 3205 Size: 663 2229 1712 3245 Fragments arranged by size: 3245 2229 1712 663 SfcI C'TryA_G Cuts at: 826 1045 2326 3538 3953 4745 4905 5197 6332 Size: 219 1281 1212 415 792 160 292 1135 Cuts at: 6332 7013 7204 826 Size: 681 191 1471 Fragments arranged by size: 1471 1281 1212 1135 792 681 415 292 219 191 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7849 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7849 SmaI CCC'GGG Cuts at: 1872 5176 1872 Size: 3304 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2648 3647 5953 6854 7116 820 Size: 197 926 705 999 2306 901 262 1553 Fragments arranged by size: 2306 1553 999 926 901 705 262 197 SnaBI TAC'GTA Cuts at: 493 5146 5640 493 Size: 4653 494 2702 Fragments arranged by size: 4653 2702 494 SpeI A'CTAG_T Cuts at: 152 2830 152 Size: 2678 5171 SphI G_CATG'C Cuts at: 1132 5207 1132 Size: 4075 3774 SspI AAT'ATT Cuts at: 5 52 2113 3633 5111 5773 5 Size: 47 2061 1520 1478 662 2081 Fragments arranged by size: 2081 2061 1520 1478 662 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7849 StyI C'CwwG_G Cuts at: 513 1065 2221 3675 5288 513 Size: 552 1156 1454 1613 3074 Fragments arranged by size: 3074 1613 1454 1156 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4350 5876 5893 6046 6231 6676 325 Size: 1255 2770 1526 17 153 185 445 1498 Fragments arranged by size: 2770 1526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3203 3227 3605 Size: 274 80 33 51 1443 1226 24 378 Cuts at: 3605 3866 4527 6095 7807 96 Size: 261 661 1568 1712 138 Fragments arranged by size: 1712 1568 1443 1226 661 378 274 261 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3950 3982 7809 7841 3950 Size: 32 3827 32 3958 Fragments arranged by size: 3958 3827 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2626 3066 1316 Size: 1310 440 6099 Fragments arranged by size: 6099 1310 440 XbaI T'CTAG_A Cuts at: 5185 5185 Size: 7849 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7849 XmaI C'CCGG_G Cuts at: 1870 5174 1870 Size: 3304 4545 XmnI GAAnn'nnTTC Cuts at: 3113 4679 4972 5978 3113 Size: 1566 293 1006 4984 Fragments arranged by size: 4984 1566 1006 293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5657 276 Size: 53 83 186 936 4123 2468 Fragments arranged by size: 4123 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI