Restriction map for
pFN21ASDA0083
(Linear) (Six-base) MAPSORT of: KIAA0083.seq Check: 4731 from: 1 to: 3180
KIAA0083.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1531 3180
Size: 1531 1649
AatII G_ACGT'C
Cuts at: 0 2997 3180
Size: 2997 183
AccI GT'mk_AC
Cuts at: 0 2897 3180
Size: 2897 283
Acc65I G'GTAC_C
Cuts at: 0 137 3180
Size: 137 3043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1318 3180
Size: 1318 1862
AflII C'TTAA_G
Cuts at: 0 2505 3180
Size: 2505 675
AflIII A'CryG_T
Cuts at: 0 748 1458 1751 2224 3180
Size: 748 710 293 473 956
Fragments arranged by size:
956 748 710 473 293
AgeI A'CCGG_T
Cuts at: 0 134 3180
Size: 134 3046
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 224 256 3180
Size: 224 32 2924
Fragments arranged by size:
2924 224 32
AlwNI CAG_nnn'CTG
Cuts at: 0 988 1296 3180
Size: 988 308 1884
Fragments arranged by size:
1884 988 308
ApaI G_GGCC'C
Cuts at: 0 2323 3180
Size: 2323 857
ApoI r'AATT_y
Cuts at: 0 915 1988 2147 2261 2611 3180
Size: 915 1073 159 114 350 569
Fragments arranged by size:
1073 915 569 350 159 114
AseI AT'TA_AT
Cuts at: 0 3112 3180
Size: 3112 68
AvrII C'CTAG_G
Cuts at: 0 802 3180
Size: 802 2378
BanI G'GyrC_C
Cuts at: 0 137 379 1513 3180
Size: 137 242 1134 1667
Fragments arranged by size:
1667 1134 242 137
BanII G_rGCy'C
Cuts at: 0 445 2323 2406 3180
Size: 445 1878 83 774
Fragments arranged by size:
1878 774 445 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 1135 3180
Size: 1135 2045
BciVI GTATCCnnnnn_n'
Cuts at: 0 368 1842 3180
Size: 368 1474 1338
Fragments arranged by size:
1474 1338 368
BfrBI ATG'CAT
Cuts at: 0 239 675 3180
Size: 239 436 2505
Fragments arranged by size:
2505 436 239
BglI GCCn_nnn'nGGC
Cuts at: 0 1890 3180
Size: 1890 1290
Bme1580I G_kGCm'C
Cuts at: 0 2323 3053 3180
Size: 2323 730 127
Fragments arranged by size:
2323 730 127
BmrI ACTGGGnnnn_n'
Cuts at: 0 3169 3180
Size: 3169 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1369 2458 2557 3180
Size: 40 1329 1089 99 623
Fragments arranged by size:
1329 1089 623 99 40
Bpu10I CC'TnA_GC
Cuts at: 0 2175 3180
Size: 2175 1005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 3180
Size: 991 2189
BsaHI Gr'CG_yC
Cuts at: 0 2994 3180
Size: 2994 186
BsaWI w'CCGG_w
Cuts at: 0 134 3180
Size: 134 3046
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 3180
Size: 64 3116
BseYI C'CCAG_C
Cuts at: 0 1289 3180
Size: 1289 1891
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 2494 3180
Size: 291 2203 686
Fragments arranged by size:
2203 686 291
BsiHKAI G_wGCw'C
Cuts at: 0 121 445 1209 2406 3180
Size: 121 324 764 1197 774
Fragments arranged by size:
1197 774 764 324 121
BsmI GAATG_Cn'
Cuts at: 0 1084 1862 3180
Size: 1084 778 1318
Fragments arranged by size:
1318 1084 778
Bsp1286I G_dGCh'C
Cuts at: 0 121 445 1209 2323 2406 3053 3180
Size: 121 324 764 1114 83 647 127
Fragments arranged by size:
1114 764 647 324 127 121 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1531 3180
Size: 1531 1649
BsrDI GCAATG_nn'
Cuts at: 0 2742 3180
Size: 2742 438
BsrFI r'CCGG_y
Cuts at: 0 63 134 3180
Size: 63 71 3046
Fragments arranged by size:
3046 71 63
BsrGI T'GTAC_A
Cuts at: 0 162 947 2460 3180
Size: 162 785 1513 720
Fragments arranged by size:
1513 785 720 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1743 3180
Size: 1743 1437
Bsu36I CC'TnA_GG
Cuts at: 0 244 3180
Size: 244 2936
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1912 3180
Size: 1912 1268
BtsI GCAGTG_nn'
Cuts at: 0 203 2573 3180
Size: 203 2370 607
Fragments arranged by size:
2370 607 203
DraI TTT'AAA
Cuts at: 0 2076 2589 3098 3180
Size: 2076 513 509 82
Fragments arranged by size:
2076 513 509 82
EaeI y'GGCC_r
Cuts at: 0 2558 3180
Size: 2558 622
EarI CTCTTCn'nnn_
Cuts at: 0 31 408 966 1160 1414 2186 3180
Size: 31 377 558 194 254 772 994
Fragments arranged by size:
994 772 558 377 254 194 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 82 3180
Size: 70 12 3098
Fragments arranged by size:
3098 70 12
EcoICRI GAG'CTC
Cuts at: 0 443 2404 3180
Size: 443 1961 776
Fragments arranged by size:
1961 776 443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1318 1369 2458 2557 3180
Size: 40 1278 51 1089 99 623
Fragments arranged by size:
1278 1089 623 99 51 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1952 3180
Size: 1952 1228
EcoO109I rG'GnC_Cy
Cuts at: 0 2320 3180
Size: 2320 860
EcoRI G'AATT_C
Cuts at: 0 1988 3180
Size: 1988 1192
EcoRV GAT'ATC
Cuts at: 0 285 3180
Size: 285 2895
HaeII r_GCGC'y
Cuts at: 0 195 2707 3180
Size: 195 2512 473
Fragments arranged by size:
2512 473 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 342 374 432 464 1342 1374
Size: 295 32 15 32 58 32 878 32
Cuts at: 1374 1962 1994 2275 2307 2530 2562 3112 3144
Size: 588 32 281 32 223 32 550 32
Cuts at: 3144 3180
Size: 36
Fragments arranged by size:
878 588 550 295 281 223 58 36
32 32 32 32 32 32 32 32
15
HincII GTy'rAC
Cuts at: 0 1343 2021 2046 2206 2468 3180
Size: 1343 678 25 160 262 712
Fragments arranged by size:
1343 712 678 262 160 25
HindIII A'AGCT_T
Cuts at: 0 2421 3180
Size: 2421 759
HpaI GTT'AAC
Cuts at: 0 1343 2468 3180
Size: 1343 1125 712
Fragments arranged by size:
1343 1125 712
KpnI G_GTAC'C
Cuts at: 0 141 3180
Size: 141 3039
MfeI C'AATT_G
Cuts at: 0 1200 1256 3180
Size: 1200 56 1924
Fragments arranged by size:
1924 1200 56
MscI TGG'CCA
Cuts at: 0 2560 3180
Size: 2560 620
MslI CAynn'nnrTG
Cuts at: 0 124 3180
Size: 124 3056
MspA1I CmG'CkG
Cuts at: 0 9 888 3180
Size: 9 879 2292
Fragments arranged by size:
2292 879 9
NaeI GCC'GGC
Cuts at: 0 65 3180
Size: 65 3115
NgoMIV G'CCGG_C
Cuts at: 0 63 3180
Size: 63 3117
NsiI A_TGCA'T
Cuts at: 0 241 677 3180
Size: 241 436 2503
Fragments arranged by size:
2503 436 241
NspI r_CATG'y
Cuts at: 0 369 752 1462 2228 3180
Size: 369 383 710 766 952
Fragments arranged by size:
952 766 710 383 369
PciI A'CATG_T
Cuts at: 0 748 1458 2224 3180
Size: 748 710 766 956
Fragments arranged by size:
956 766 748 710
PflMI CCAn_nnn'nTGG
Cuts at: 0 988 3159 3180
Size: 988 2171 21
Fragments arranged by size:
2171 988 21
PspOMI G'GGCC_C
Cuts at: 0 2319 3180
Size: 2319 861
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1170 1202 3180
Size: 1170 32 1978
Fragments arranged by size:
1978 1170 32
PvuII CAG'CTG
Cuts at: 0 9 3180
Size: 9 3171
SacI G_AGCT'C
Cuts at: 0 445 2406 3180
Size: 445 1961 774
Fragments arranged by size:
1961 774 445
SapI GCTCTTCn'nnn_
Cuts at: 0 408 966 2186 3180
Size: 408 558 1220 994
Fragments arranged by size:
1220 994 558 408
SexAI A'CCwGG_T
Cuts at: 0 140 3180
Size: 140 3040
SmlI C'TyrA_G
Cuts at: 0 1006 2505 3180
Size: 1006 1499 675
Fragments arranged by size:
1499 1006 675
SspI AAT'ATT
Cuts at: 0 1183 1318 1486 2052 2249 3180
Size: 1183 135 168 566 197 931
Fragments arranged by size:
1183 931 566 197 168 135
StyI C'CwwG_G
Cuts at: 0 802 2638 2907 3180
Size: 802 1836 269 273
Fragments arranged by size:
1836 802 273 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1644 3151 3180
Size: 1644 1507 29
Fragments arranged by size:
1644 1507 29
TatI w'GTAC_w
Cuts at: 0 162 947 1975 2460 2477 3180
Size: 162 785 1028 485 17 703
Fragments arranged by size:
1028 785 703 485 162 17
XbaI T'CTAG_A
Cuts at: 0 1044 1591 2184 3180
Size: 1044 547 593 996
Fragments arranged by size:
1044 996 593 547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 469 1507 3180
Size: 469 1038 1673
Fragments arranged by size:
1673 1038 469
XmnI GAAnn'nnTTC
Cuts at: 0 1731 3180
Size: 1731 1449
ZraI GAC'GTC
Cuts at: 0 2995 3180
Size: 2995 185
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AgeI
AloI AlwNI ApaI ApoI AseI AvrII BanI BanII
BbsI BciVI BfrBI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI Bsu36I BtgZI
BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MscI MslI MspA1I NaeI NgoMIV NsiI
NspI PciI PflMI PspOMI PsrI PvuII SacI SapI
SexAI SmlI SspI StyI TaqII TatI XbaI XcmI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AhdI AleI AlfI ApaLI AscI AsiSI
AvaI BaeI BamHI BbeI BbvCI BcgI BclI BglII
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaXI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI ClaI CspCI
DraIII DrdI EagI FalI FseI FspI FspAI KasI
MluI MmeI NarI NcoI NdeI NheI NotI NruI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XhoI XmaI