Restriction map for pFN21ASDA0081
 (Circular) (Six-base) MAPSORT of: pFN21ASDA0081.seq  Check: 9558  from: 1  to: 5431



pFN21ASDA0081  5431 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:29  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2463    3241     278 
   Size:         53      83     186     936     927     778    2468

  Fragments arranged by size: 

                2468     936     927     778     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2153    2295    2774    1937 
   Size:        216     142     479    4594

  Fragments arranged by size: 

                4594     479     216     142
 
Acc65I G'GTAC_C

Cuts at:   2752    2752 
   Size:       5431
 
AclI AA'CG_TT

Cuts at:   1563    3558    3931    1563 
   Size:       1995     373    3063

  Fragments arranged by size: 

                3063    1995     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2268    3494    4878    5146    1632 
   Size:        636    1226    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1226     636     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5234
 
AflIII A'CryG_T

Cuts at:   1203    4330    5307    1203 
   Size:       3127     977    1327

  Fragments arranged by size: 

                3127    1327     977
 
AgeI A'CCGG_T

Cuts at:   4262    4262 
   Size:       5431
 
AhdI GACnn_n'nnGTC

Cuts at:   4160    4160 
   Size:       5431
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5431
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5431
 
AlwNI CAG_nnn'CTG

Cuts at:   2291    3174    4746    2291 
   Size:        883    1572    2976

  Fragments arranged by size: 

                2976    1572     883
 
ApaLI G'TGCA_C

Cuts at:   3488    4644    3488 
   Size:       1156    4275
 
ApoI r'AATT_y

Cuts at:   1493    2740    2966    3118    5227    1493 
   Size:       1247     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1247     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3985     160 
   Size:        872    2953    1606

  Fragments arranged by size: 

                2953    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5431
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2135    2756    1870 
   Size:         73     192     621    4545

  Fragments arranged by size: 

                4545     621     192      73
 
BamHI G'GATC_C

Cuts at:   2761    3128    2761 
   Size:        367    5064
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2752    4207     618 
   Size:        325     217     627     965    1455    1842

  Fragments arranged by size: 

                1842    1455     965     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2750    1391 
   Size:       1359    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2210    2363    2673    2724     928 
   Size:        971     311     153     310      51    3635

  Fragments arranged by size: 

                3635     971     311     310     153      51
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3622    3656    1903 
   Size:         34    1685      34    3678

  Fragments arranged by size: 

                3678    1685      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3323    4533    3323 
   Size:       1210    4221
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5431
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4042     136 
   Size:        107     122      71     631     605    2370    1525

  Fragments arranged by size: 

                2370    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2260    2665    5426    2260 
   Size:        405    2761    2265

  Fragments arranged by size: 

                2761    2265     405
 
BlpI GC'TnA_GC

Cuts at:   2848    2848 
   Size:       5431
 
Bme1580I G_kGCm'C

Cuts at:   1790    3492    4648    1790 
   Size:       1702    1156    2573

  Fragments arranged by size: 

                2573    1702    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5431
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3162    4120     442 
   Size:        416      53      83     399      24    1745     958    1753

  Fragments arranged by size: 

                1753    1745     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5431
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2243    2597    4091    1239 
   Size:       1004     354    1494    2579

  Fragments arranged by size: 

                2579    1494    1004     354
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3556    4421    4719    3556 
   Size:        865     298    4268

  Fragments arranged by size: 

                4268     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2272    4094     882 
   Size:        617     773    1822    2219

  Fragments arranged by size: 

                2219    1822     773     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2728    3222     493 
   Size:        711    1524     494    2702

  Fragments arranged by size: 

                2702    1524     711     494
 
BsaBI GATnn'nnATC

Cuts at:   5425    5425 
   Size:       5431
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2141    2460    3238    3620 
   Size:         53      83     186     936     608     319     778     382

Cuts at:   3620    4255     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     778     635     608     382     319     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   3864    4262    4536    4683    5094    3864 
   Size:        398     274     147     411    4201

  Fragments arranged by size: 

                4201     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2146    2176    1178 
   Size:         30     165      30     743      30    4433

  Fragments arranged by size: 

                4433     743     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2619    1186 
   Size:       1433    3998
 
BseYI C'CCAG_C

Cuts at:   2702    4634    5130    2702 
   Size:       1932     496    3003

  Fragments arranged by size: 

                3003    1932     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4858
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2124    2881    3642    4670    1540 
   Size:        275     186     123     757     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     757     275     186     123
 
BsiHKAI G_wGCw'C

Cuts at:   2274    3492    3577    4648    2274 
   Size:       1218      85    1071    3057

  Fragments arranged by size: 

                3057    1218    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2942    3035    2942 
   Size:         93    5338
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2274    2750    3492    3577    4648    1391 
   Size:        399     484     476     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     484     476     399      85
 
BspEI T'CCGG_A

Cuts at:   5094    5094 
   Size:       5431
 
BspHI T'CATG_A

Cuts at:   2364    3320    5050    2364 
   Size:        956    1730    2745

  Fragments arranged by size: 

                2745    1730     956
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2085    2786     844 
   Size:       1241     701    3489

  Fragments arranged by size: 

                3489    1241     701
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3318    1394 
   Size:        429    1495    3507

  Fragments arranged by size: 

                3507    1495     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2234    3926    4100      65 
   Size:       1183     986    1692     174    1396

  Fragments arranged by size: 

                1692    1396    1183     986     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4075    4262    1700 
   Size:        110    2265     187    2869

  Fragments arranged by size: 

                2869    2265     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5431
 
BssHII G'CGCG_C

Cuts at:   1925    2042    1925 
   Size:        117    5314
 
BssSI C'ACGA_G

Cuts at:   3491    4503    3491 
   Size:       1012    4419
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5431
 
BstBI TT'CG_AA

Cuts at:   2007    4235    2007 
   Size:       2228    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5431
 
BstYI r'GATC_y

Cuts at:   1972    2260    2665    2761    3128    3513    3530    4982    5068 
   Size:        288     405      96     367     385      17    1452      86

Cuts at:   5068    5080    5426    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     405     385     367     346     288      96
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2022    2052     513 
   Size:        552     957      30    3892

  Fragments arranged by size: 

                3892     957     552      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2523     487 
   Size:        683      56    1297    3395

  Fragments arranged by size: 

                3395    1297     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2947    3741    3761    2947 
   Size:        794      20    4617

  Fragments arranged by size: 

                4617     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5431
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5396
 
DraI TTT'AAA

Cuts at:   2735    2735 
   Size:       5431
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2543    4438     809 
   Size:        507    1227    1895    1802

  Fragments arranged by size: 

                1895    1802    1227     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2121    2878    3767 
   Size:         54    1425      50     275      18     291     757     889

Cuts at:   3767    5396       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     757     291     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2121    2878    1537 
   Size:        275     309     757    4090

  Fragments arranged by size: 

                4090     757     309     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3361    1353 
   Size:       2008    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4018    4392    4538     387 
   Size:       1064     138    2429     374     146    1280

  Fragments arranged by size: 

                2429    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2243    2268    2597    3494    4091    4878    5146 
   Size:        393     611      25     329     897     597     787     268

Cuts at:   5146    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     897     787     611     597     393     329     268
                  25
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2429    2429 
   Size:       5431
 
EcoO109I rG'GnC_Cy

Cuts at:   2112    2112 
   Size:       5431
 
EcoRI G'AATT_C

Cuts at:   2740    3118    2740 
   Size:        378    5053
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2279    2279 
   Size:       5431
 
FspI TGC'GCA

Cuts at:   3937    3937 
   Size:       5431
 
HaeII r_GCGC'y

Cuts at:   1678    4578    1678 
   Size:       2900    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2657    2689    4072    4104    4146 
   Size:         32     135      32    1085      32    1383      32      42

Cuts at:   4146    4178    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1383    1085     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2296    2775    3021     669 
   Size:        988     281     358     479     246    3079

  Fragments arranged by size: 

                3079     988     479     358     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5431
 
HpaI GTT'AAC

Cuts at:   3021    3021 
   Size:       5431
 
KpnI G_GTAC'C

Cuts at:   2756    2756 
   Size:       5431
 
MfeI C'AATT_G

Cuts at:   3030    3030 
   Size:       5431
 
MluI A'CGCG_T

Cuts at:   5307    5307 
   Size:       5431
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4545    4729    1305 
   Size:       3240     184    2007

  Fragments arranged by size: 

                3240    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3609

  Fragments arranged by size: 

                3609    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3389    3748    3907     518 
   Size:        648    2223     359     159    2042

  Fragments arranged by size: 

                2223    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2847    3524    4672    4917    1606 
   Size:       1241     677    1148     245    2120

  Fragments arranged by size: 

                2120    1241    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2022     513 
   Size:        552     957    3922

  Fragments arranged by size: 

                3922     957     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5431
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5431
 
NotI GC'GGCC_GC

Cuts at:   2878    2878 
   Size:       5431
 
NruI TCG'CGA

Cuts at:   2190    2508    2190 
   Size:        318    5113
 
NspI r_CATG'y

Cuts at:   1132    1229    2789    4334    1132 
   Size:         97    1560    1545    2229

  Fragments arranged by size: 

                2229    1560    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5431
 
PciI A'CATG_T

Cuts at:   4330    4330 
   Size:       5431
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2108    1108 
   Size:       1000    4431
 
PmeI GTTT'AAAC

Cuts at:   2735    2735 
   Size:       5431
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3500    3532    5013    5045    3500 
   Size:         32    1481      32    3886

  Fragments arranged by size: 

                3886    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2112    2112 
   Size:       5431
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5431
 
PsiI TTA'TAA

Cuts at:   3001    3001 
   Size:       5431
 
PstI C_TGCA'G

Cuts at:    830    2783     830 
   Size:       1953    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5431
 
SalI G'TCGA_C

Cuts at:   1936    2294    2773    1936 
   Size:        358     479    4594

  Fragments arranged by size: 

                4594     479     358
 
SbfI CC_TGCA'GG

Cuts at:   2783    2783 
   Size:       5431
 
ScaI AGT'ACT

Cuts at:   3679    5391    3679 
   Size:       1712    3719
 
SfcI C'TryA_G

Cuts at:    826    1045    2779    3914    4595    4786     826 
   Size:        219    1734    1135     681     191    1471

  Fragments arranged by size: 

                1734    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5431
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5431
 
SmaI CCC'GGG

Cuts at:   1872    2137    2758    1872 
   Size:        265     621    4545

  Fragments arranged by size: 

                4545     621     265
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3535    4436    4698     820 
   Size:        197     926    1592     901     262    1553

  Fragments arranged by size: 

                1592    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2728    3222     493 
   Size:       2235     494    2702

  Fragments arranged by size: 

                2702    2235     494
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5431
 
SphI G_CATG'C

Cuts at:   1132    2789    1132 
   Size:       1657    3774
 
SspI AAT'ATT

Cuts at:      5      52    3355       5 
   Size:         47    3303    2081

  Fragments arranged by size: 

                3303    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5431
 
StyI C'CwwG_G

Cuts at:    513    1065    2022    2685    2870     513 
   Size:        552     957     663     185    3074

  Fragments arranged by size: 

                3074     957     663     552     185
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2537    2667    3458    3475    3628    3813    4258 
   Size:       1255     957     130     791      17     153     185     445

Cuts at:   4258     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255     957     791     445     185     153     130
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3677    5389      96 
   Size:        274      80      33      51    1443    1700    1712     138

  Fragments arranged by size: 

                1712    1700    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5391    5423    5391 
   Size:         32    5399
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5431
 
XbaI T'CTAG_A

Cuts at:   2767    2767 
   Size:       5431
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5431
 
XmaI C'CCGG_G

Cuts at:   1870    2135    2756    1870 
   Size:        265     621    4545

  Fragments arranged by size: 

                4545     621     265
 
XmnI GAAnn'nnTTC

Cuts at:   3560    3560 
   Size:       5431
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2461    3239     276 
   Size:         53      83     186     936     927     778    2468

  Fragments arranged by size: 

                2468     936     927     778     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NruI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoRV     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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