Restriction map for
pFN21ASDA0081
(Linear) (Six-base) MAPSORT of: KIAA0081.seq Check: 6059 from: 1 to: 702
KIAA0081.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AatII G_ACGT'C
Cuts at: 0 440 702
Size: 440 262
AccI GT'mk_AC
Cuts at: 0 130 272 702
Size: 130 142 430
Fragments arranged by size:
430 142 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 702
Size: 245 457
AlwNI CAG_nnn'CTG
Cuts at: 0 268 702
Size: 268 434
AvaI C'yCGr_G
Cuts at: 0 112 702
Size: 112 590
BbsI GAAGACnn'nnnn_
Cuts at: 0 187 340 650 701 702
Size: 187 153 310 51 1
Fragments arranged by size:
310 187 153 51 1
BglII A'GATC_T
Cuts at: 0 237 642 702
Size: 237 405 60
Fragments arranged by size:
405 237 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 574 702
Size: 220 354 128
Fragments arranged by size:
354 220 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 249 702
Size: 249 453
BsaHI Gr'CG_yC
Cuts at: 0 118 437 702
Size: 118 319 265
Fragments arranged by size:
319 265 118
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 123 153 702
Size: 123 30 549
Fragments arranged by size:
549 123 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 596 702
Size: 596 106
BseYI C'CCAG_C
Cuts at: 0 679 702
Size: 679 23
BsiEI CG_ry'CG
Cuts at: 0 101 702
Size: 101 601
BsiHKAI G_wGCw'C
Cuts at: 0 251 702
Size: 251 451
Bsp1286I G_dGCh'C
Cuts at: 0 251 702
Size: 251 451
BspHI T'CATG_A
Cuts at: 0 341 702
Size: 341 361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 702
Size: 62 640
BsrDI GCAATG_nn'
Cuts at: 0 211 702
Size: 211 491
BssHII G'CGCG_C
Cuts at: 0 19 702
Size: 19 683
BstYI r'GATC_y
Cuts at: 0 237 642 702
Size: 237 405 60
Fragments arranged by size:
405 237 60
BtgI C'CryG_G
Cuts at: 0 29 702
Size: 29 673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 500 702
Size: 500 202
DrdI GACnn_nn'nnGTC
Cuts at: 0 520 702
Size: 520 182
EaeI y'GGCC_r
Cuts at: 0 98 702
Size: 98 604
EagI C'GGCC_G
Cuts at: 0 98 702
Size: 98 604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 245 574 702
Size: 220 25 329 128
Fragments arranged by size:
329 220 128 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 702
Size: 406 296
EcoO109I rG'GnC_Cy
Cuts at: 0 89 702
Size: 89 613
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 256 702
Size: 256 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 634 666 702
Size: 634 32 36
Fragments arranged by size:
634 36 32
HincII GTy'rAC
Cuts at: 0 273 702
Size: 273 429
NruI TCG'CGA
Cuts at: 0 167 485 702
Size: 167 318 217
Fragments arranged by size:
318 217 167
PflMI CCAn_nnn'nTGG
Cuts at: 0 85 702
Size: 85 617
PpuMI rG'GwC_Cy
Cuts at: 0 89 702
Size: 89 613
SalI G'TCGA_C
Cuts at: 0 271 702
Size: 271 431
SmaI CCC'GGG
Cuts at: 0 114 702
Size: 114 588
StyI C'CwwG_G
Cuts at: 0 662 702
Size: 662 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 514 644 702
Size: 514 130 58
Fragments arranged by size:
514 130 58
XmaI C'CCGG_G
Cuts at: 0 112 702
Size: 112 590
ZraI GAC'GTC
Cuts at: 0 438 702
Size: 438 264
Enzymes that do cut:
AatII AccI AcuI AlwNI AvaI BbsI BglII BpmI
BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BssHII BstYI BtgI BtgZI DrdI
EaeI EagI Eco57MI EcoNI EcoO109I FalI Hin4I HincII
NruI PflMI PpuMI SalI SmaI StyI TaqII XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsgI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII EarI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmnI