(Circular) (Six-base) MAPSORT of: pFN21ASDA0080.seq Check: 632 from: 1 to: 6022 pFN21ASDA0080 6022 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3030 3365 1937 Size: 1093 335 4594 Fragments arranged by size: 4594 1093 335 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4085 5469 5737 1632 Size: 2453 1384 268 1917 Fragments arranged by size: 2453 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 2065 2993 4853 2065 Size: 928 1860 3234 Fragments arranged by size: 3234 1860 928 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6022 AlwNI CAG_nnn'CTG Cuts at: 2324 2740 3765 5337 2324 Size: 416 1025 1572 3009 Fragments arranged by size: 3009 1572 1025 416 ApaLI G'TGCA_C Cuts at: 3079 4079 5235 3079 Size: 1000 1156 3866 Fragments arranged by size: 3866 1156 1000 ApoI r'AATT_y Cuts at: 1493 3331 3557 3709 5818 1493 Size: 1838 226 152 2109 1697 Fragments arranged by size: 2109 1838 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2781 3347 1870 Size: 73 838 566 4545 Fragments arranged by size: 4545 838 566 73 BamHI G'GATC_C Cuts at: 2498 3352 3719 2498 Size: 854 367 4801 Fragments arranged by size: 4801 854 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2809 3343 4798 618 Size: 325 217 627 1022 534 1455 1842 Fragments arranged by size: 1842 1455 1022 627 534 325 217 BanII G_rGCy'C Cuts at: 1391 2426 2907 3275 3341 1391 Size: 1035 481 368 66 4072 Fragments arranged by size: 4072 1035 481 368 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2660 2678 928 Size: 971 761 18 4272 Fragments arranged by size: 4272 971 761 18 BbvCI CC'TCA_GC Cuts at: 2228 2762 2228 Size: 534 5488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4213 4247 1903 Size: 34 2276 34 3678 Fragments arranged by size: 3678 2276 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 1548 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4633 136 Size: 107 122 71 631 605 2961 1525 Fragments arranged by size: 2961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 2333 3439 2333 Size: 1106 4916 Bme1580I G_kGCm'C Cuts at: 1790 3083 3223 4083 5239 1790 Size: 1293 140 860 1156 2573 Fragments arranged by size: 2573 1293 1156 860 140 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6022 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3753 4711 442 Size: 416 53 83 399 24 2336 958 1753 Fragments arranged by size: 2336 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2921 2921 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2460 2891 3274 4682 1239 Size: 1221 431 383 1408 2579 Fragments arranged by size: 2579 1408 1221 431 383 Bpu10I CC'TnA_GC Cuts at: 2228 2762 2228 Size: 534 5488 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4147 5012 5310 4147 Size: 865 298 4859 Fragments arranged by size: 4859 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 2690 3002 4685 882 Size: 617 717 474 312 1683 2219 Fragments arranged by size: 2219 1683 717 617 474 312 BsaAI yAC'GTr Cuts at: 493 1204 3319 3813 493 Size: 711 2115 494 2702 Fragments arranged by size: 2702 2115 711 494 BsaBI GATnn'nnATC Cuts at: 2987 6016 2987 Size: 3029 2993 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3829 4211 4846 275 Size: 53 83 186 936 2296 382 635 1451 Fragments arranged by size: 2296 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2065 2993 4455 4853 5127 5274 5685 2065 Size: 928 1462 398 274 147 411 2402 Fragments arranged by size: 2402 1462 928 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2272 2302 2449 2479 1178 Size: 30 165 30 869 30 147 30 4721 Fragments arranged by size: 4721 869 165 147 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 1186 Size: 1309 4713 BseYI C'CCAG_C Cuts at: 2831 5225 5721 2831 Size: 2394 496 3132 Fragments arranged by size: 3132 2394 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2732 1163 Size: 573 996 4453 Fragments arranged by size: 4453 996 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3472 4233 5261 1540 Size: 275 186 1177 294 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 294 275 186 BsiHKAI G_wGCw'C Cuts at: 2907 3083 4083 4168 5239 2907 Size: 176 1000 85 1071 3690 Fragments arranged by size: 3690 1071 1000 176 85 BsmI GAATG_Cn' Cuts at: 3533 3626 3533 Size: 93 5929 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2426 2907 3083 3223 3275 3341 4083 Size: 399 636 481 176 140 52 66 742 Cuts at: 4083 4168 5239 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 636 481 399 176 140 85 66 52 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 3911 5641 3911 Size: 1730 4292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3377 844 Size: 2533 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3909 1394 Size: 429 2086 3507 Fragments arranged by size: 3507 2086 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3048 4517 4691 65 Size: 1183 1800 1469 174 1396 Fragments arranged by size: 1800 1469 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2075 2993 4666 4853 1700 Size: 110 255 10 918 1673 187 2869 Fragments arranged by size: 2869 1673 918 255 187 110 10 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6022 BssSI C'ACGA_G Cuts at: 4082 5094 4082 Size: 1012 5010 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 2007 4826 2007 Size: 2819 3203 BstEII G'GTnAC_C Cuts at: 1184 2116 1184 Size: 932 5090 BstXI CCAn_nnnn'nTGG Cuts at: 2398 2398 Size: 6022 BstYI r'GATC_y Cuts at: 1972 2347 2369 2498 2600 3352 3719 4104 4121 Size: 375 22 129 102 752 367 385 17 Cuts at: 4121 5573 5659 5671 6017 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 752 385 375 367 346 129 102 86 22 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2566 513 Size: 552 957 544 3969 Fragments arranged by size: 3969 957 552 544 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5283 Fragments arranged by size: 5283 683 56 BtsI GCAGTG_nn' Cuts at: 3538 4332 4352 3538 Size: 794 20 5208 Fragments arranged by size: 5208 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DraIII CAC_nnn'GTG Cuts at: 3227 3227 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5029 809 Size: 507 3713 1802 Fragments arranged by size: 3713 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 3288 3469 Size: 54 1425 50 275 18 243 1215 181 Cuts at: 3469 4358 5987 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1215 889 275 243 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3469 1537 Size: 275 1657 4090 Fragments arranged by size: 4090 1657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3952 1353 Size: 2599 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4609 4983 5129 387 Size: 1064 138 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2905 2905 Size: 6022 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2460 2891 3274 4085 4682 5469 5737 Size: 393 828 431 383 811 597 787 268 Cuts at: 5737 1239 Size: 1524 Fragments arranged by size: 1524 828 811 787 597 431 393 383 268 EcoO109I rG'GnC_Cy Cuts at: 2088 2357 2737 2088 Size: 269 380 5373 Fragments arranged by size: 5373 380 269 EcoRI G'AATT_C Cuts at: 3331 3709 3331 Size: 378 5644 EcoRV GAT'ATC Cuts at: 2989 3145 2989 Size: 156 5866 FseI GG_CCGG'CC Cuts at: 2079 2079 Size: 6022 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 5169 1678 Size: 3491 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2762 2794 4663 4695 4737 Size: 32 135 32 1190 32 1869 32 42 Cuts at: 4737 4769 1373 Size: 32 2626 Fragments arranged by size: 2626 1869 1190 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3612 669 Size: 988 281 1428 246 3079 Fragments arranged by size: 3079 1428 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2068 5136 5320 1305 Size: 763 3068 184 2007 Fragments arranged by size: 3068 2007 763 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3290 10 Size: 54 1425 343 1458 2742 Fragments arranged by size: 2742 1458 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2252 2396 2618 3980 4339 4498 518 Size: 648 1086 144 222 1362 359 159 2042 Fragments arranged by size: 2042 1362 1086 648 359 222 159 144 MspA1I CmG'CkG Cuts at: 1606 2731 3438 4115 5263 5508 1606 Size: 1125 707 677 1148 245 2120 Fragments arranged by size: 2120 1148 1125 707 677 245 NaeI GCC'GGC Cuts at: 2077 2077 Size: 6022 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4513 Fragments arranged by size: 4513 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NgoMIV G'CCGG_C Cuts at: 2075 2075 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 2197 3380 4925 1132 Size: 97 968 1183 1545 2229 Fragments arranged by size: 2229 1545 1183 968 97 PasI CC'CwG_GG Cuts at: 1348 2352 2444 2596 1348 Size: 1004 92 152 4774 Fragments arranged by size: 4774 1004 152 92 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6022 PfoI T'CCnGG_A Cuts at: 2556 2556 Size: 6022 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2737 2737 Size: 6022 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2162 2194 3017 3049 2162 Size: 32 823 32 5135 Fragments arranged by size: 5135 823 32 32 PstI C_TGCA'G Cuts at: 830 2736 3374 830 Size: 1906 638 3478 Fragments arranged by size: 3478 1906 638 PvuI CG_AT'CG Cuts at: 2001 3178 2001 Size: 1177 4845 PvuII CAG'CTG Cuts at: 2731 2731 Size: 6022 SacI G_AGCT'C Cuts at: 2907 2907 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 3314 4270 5982 3314 Size: 956 1712 3354 Fragments arranged by size: 3354 1712 956 SexAI A'CCwGG_T Cuts at: 2739 2739 Size: 6022 SfcI C'TryA_G Cuts at: 826 1045 2722 2732 3370 4505 5186 5377 826 Size: 219 1677 10 638 1135 681 191 1471 Fragments arranged by size: 1677 1471 1135 681 638 219 191 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 2065 1700 Size: 365 5657 SmaI CCC'GGG Cuts at: 1872 3349 1872 Size: 1477 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4126 5027 5289 820 Size: 197 926 2183 901 262 1553 Fragments arranged by size: 2183 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3319 3813 493 Size: 2826 494 2702 Fragments arranged by size: 2826 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 2022 3461 513 Size: 552 957 1439 3074 Fragments arranged by size: 3074 1439 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4049 4066 4219 4404 4849 325 Size: 1255 2469 17 153 185 445 1498 Fragments arranged by size: 2469 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3312 4268 5980 Size: 274 80 33 51 1443 1335 956 1712 Cuts at: 5980 96 Size: 138 Fragments arranged by size: 1712 1443 1335 956 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2272 2304 5982 6014 2272 Size: 32 3678 32 2280 Fragments arranged by size: 3678 2280 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3193 3193 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 3347 1870 Size: 1477 4545 XmnI GAAnn'nnTTC Cuts at: 4151 4151 Size: 6022 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI