(Circular) (Six-base) MAPSORT of: pFN21ASDA0076.seq Check: 7894 from: 1 to: 9823 pFN21ASDA0076 9823 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5325 5473 5325 Size: 148 9675 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6885 7633 278 Size: 53 83 186 936 5349 748 2468 Fragments arranged by size: 5349 2468 936 748 186 83 53 AccI GT'mk_AC Cuts at: 1937 3714 5982 7166 1937 Size: 1777 2268 1184 4594 Fragments arranged by size: 4594 2268 1777 1184 Acc65I G'GTAC_C Cuts at: 7144 7144 Size: 9823 AclI AA'CG_TT Cuts at: 1563 7950 8323 1563 Size: 6387 373 3063 Fragments arranged by size: 6387 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3058 3753 4112 5660 6479 7886 9270 9538 Size: 1426 695 359 1548 819 1407 1384 268 Cuts at: 9538 1632 Size: 1917 Fragments arranged by size: 1917 1548 1426 1407 1384 819 695 359 268 AfeI AGC'GCT Cuts at: 4874 4874 Size: 9823 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9626 AflIII A'CryG_T Cuts at: 1203 2526 8722 9699 1203 Size: 1323 6196 977 1327 Fragments arranged by size: 6196 1327 1323 977 AgeI A'CCGG_T Cuts at: 8654 8654 Size: 9823 AhdI GACnn_n'nnGTC Cuts at: 8552 8552 Size: 9823 AleI CACnn'nnGTG Cuts at: 1166 5670 1166 Size: 4504 5319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5109 1184 Size: 3925 5898 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2891 2923 4334 4366 5171 5203 6281 6313 6995 Size: 32 1411 32 805 32 1078 32 682 Cuts at: 6995 7027 2891 Size: 32 5687 Fragments arranged by size: 5687 1411 1078 805 682 32 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2676 3892 4039 4204 4336 5239 5944 5998 6258 Size: 1216 147 165 132 903 705 54 260 Cuts at: 6258 6409 6952 6968 7566 9138 2676 Size: 151 543 16 598 1572 3361 Fragments arranged by size: 3361 1572 1216 903 705 598 543 260 165 151 147 132 54 16 ApaI G_GGCC'C Cuts at: 2070 2295 2326 6534 6821 2070 Size: 225 31 4208 287 5072 Fragments arranged by size: 5072 4208 287 225 31 ApaLI G'TGCA_C Cuts at: 3290 7880 9036 3290 Size: 4590 1156 4077 Fragments arranged by size: 4590 4077 1156 ApoI r'AATT_y Cuts at: 1493 2048 5089 7132 7358 7510 9619 1493 Size: 555 3041 2043 226 152 2109 1697 Fragments arranged by size: 3041 2109 2043 1697 555 226 152 AseI AT'TA_AT Cuts at: 160 1032 8377 160 Size: 872 7345 1606 Fragments arranged by size: 7345 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9823 AvaI C'yCGr_G Cuts at: 1870 1943 4112 4714 4731 5418 5589 5863 7062 Size: 73 2169 602 17 687 171 274 1199 Cuts at: 7062 7148 1870 Size: 86 4545 Fragments arranged by size: 4545 2169 1199 687 602 274 171 86 73 17 AvrII C'CTAG_G Cuts at: 4921 4921 Size: 9823 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6428 6461 6428 Size: 33 9790 BamHI G'GATC_C Cuts at: 7153 7520 7153 Size: 367 9456 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2924 5344 7144 8599 Size: 325 217 627 269 868 2420 1800 1455 Cuts at: 8599 618 Size: 1842 Fragments arranged by size: 2420 1842 1800 1455 868 627 325 269 217 BanII G_rGCy'C Cuts at: 1391 2070 2186 2295 2326 2386 2478 2851 2990 Size: 679 116 109 31 60 92 373 139 Cuts at: 2990 3041 3048 3397 4514 5337 5408 5731 6501 Size: 51 7 349 1117 823 71 323 770 Cuts at: 6501 6534 6821 7142 1391 Size: 33 287 321 4072 Fragments arranged by size: 4072 1117 823 770 679 373 349 323 321 287 139 116 109 92 71 60 51 33 31 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3333 4446 5251 6693 928 Size: 971 1434 1113 805 1442 4058 Fragments arranged by size: 4058 1442 1434 1113 971 805 BbvCI CC'TCA_GC Cuts at: 3021 3947 6678 3021 Size: 926 2731 6166 Fragments arranged by size: 6166 2731 926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2816 2850 4705 4739 4949 4983 5701 Size: 34 879 34 1855 34 210 34 718 Cuts at: 5701 5735 8014 8048 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 1855 879 718 210 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6019 7715 8925 6019 Size: 1696 1210 6917 Fragments arranged by size: 6917 1696 1210 BclI T'GATC_A Cuts at: 1548 3699 1548 Size: 2151 7672 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2065 3406 4157 Size: 107 122 71 631 605 393 1341 751 Cuts at: 4157 8434 136 Size: 4277 1525 Fragments arranged by size: 4277 1525 1341 751 631 605 393 122 107 71 BglII A'GATC_T Cuts at: 2241 3309 9818 2241 Size: 1068 6509 2246 Fragments arranged by size: 6509 2246 1068 BlpI GC'TnA_GC Cuts at: 2208 2453 2989 3063 6384 7240 2208 Size: 245 536 74 3321 856 4791 Fragments arranged by size: 4791 3321 856 536 245 74 Bme1580I G_kGCm'C Cuts at: 1790 2061 2070 2295 2326 2414 2927 3294 3630 Size: 271 9 225 31 88 513 367 336 Cuts at: 3630 3688 5002 6284 6534 6821 6920 7074 7884 Size: 58 1314 1282 250 287 99 154 810 Cuts at: 7884 9040 1790 Size: 1156 2573 Fragments arranged by size: 2573 1314 1282 1156 810 513 367 336 287 271 250 225 154 99 88 58 31 9 BmgBI CAC'GTC Cuts at: 1318 5980 6369 1318 Size: 4662 389 4772 Fragments arranged by size: 4772 4662 389 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7554 8512 442 Size: 416 53 83 399 24 6137 958 1753 Fragments arranged by size: 6137 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2690 1056 Size: 1634 8189 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4579 6361 6611 4211 Size: 368 1782 250 7423 Fragments arranged by size: 7423 1782 368 250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2417 2505 2578 2918 2924 3326 3433 Size: 1124 54 88 73 340 6 402 107 Cuts at: 3433 3545 3554 4181 4340 4498 4847 5029 5261 Size: 112 9 627 159 158 349 182 232 Cuts at: 5261 5422 5894 5909 5975 6041 6821 8483 1239 Size: 161 472 15 66 66 780 1662 2579 Fragments arranged by size: 2579 1662 1124 780 627 472 402 349 340 232 182 161 159 158 112 107 88 73 66 66 54 15 9 6 Bpu10I CC'TnA_GC Cuts at: 3021 3816 3947 4329 5945 6678 3021 Size: 795 131 382 1616 733 6166 Fragments arranged by size: 6166 1616 795 733 382 131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5551 6356 6581 6731 7948 8813 9111 5551 Size: 805 225 150 1217 865 298 6263 Fragments arranged by size: 6263 1217 865 805 298 225 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5866 6472 8486 882 Size: 617 4367 606 2014 2219 Fragments arranged by size: 4367 2219 2014 617 606 BsaAI yAC'GTr Cuts at: 493 1204 2449 5668 7120 7614 493 Size: 711 1245 3219 1452 494 2702 Fragments arranged by size: 3219 2702 1452 1245 711 494 BsaBI GATnn'nnATC Cuts at: 2124 9817 2124 Size: 7693 2130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6882 6928 7630 8012 Size: 53 83 186 936 5349 46 702 382 Cuts at: 8012 8647 275 Size: 635 1451 Fragments arranged by size: 5349 1451 936 702 635 382 186 83 53 46 BsaWI w'CCGG_w Cuts at: 4048 7113 8256 8654 8928 9075 9486 4048 Size: 3065 1143 398 274 147 411 4385 Fragments arranged by size: 4385 3065 1143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2404 2434 2848 2878 2891 Size: 30 165 30 1001 30 414 30 13 Cuts at: 2891 2921 4110 4140 4271 4301 4523 4553 5171 Size: 30 1189 30 131 30 222 30 618 Cuts at: 5171 5201 5390 5420 6281 6311 6872 6902 6995 Size: 30 189 30 861 30 561 30 93 Cuts at: 6995 7025 1178 Size: 30 3976 Fragments arranged by size: 3976 1189 1001 861 618 561 414 222 189 165 131 93 30 30 30 30 30 30 30 30 30 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2360 2414 2418 2885 3206 3488 3503 5358 Size: 1174 54 4 467 321 282 15 1855 Cuts at: 5358 6149 6158 6161 7003 1186 Size: 791 9 3 842 4006 Fragments arranged by size: 4006 1855 1174 842 791 467 321 282 54 15 9 4 3 BseYI C'CCAG_C Cuts at: 2266 2600 2958 3914 4267 4426 4542 5148 5281 Size: 334 358 956 353 159 116 606 133 Cuts at: 5281 5925 6098 6373 6378 9026 9522 2266 Size: 644 173 275 5 2648 496 2567 Fragments arranged by size: 2648 2567 956 644 606 496 358 353 334 275 173 159 133 116 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2583 3272 4052 4202 4640 5030 5195 Size: 573 847 689 780 150 438 390 165 Cuts at: 5195 6446 6755 7069 1163 Size: 1251 309 314 3917 Fragments arranged by size: 3917 1251 847 780 689 573 438 390 314 309 165 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6940 7273 8034 9062 1540 Size: 275 186 4939 333 761 1028 2301 Fragments arranged by size: 4939 2301 1028 761 333 275 186 BsiHKAI G_wGCw'C Cuts at: 2214 2454 2683 2772 2990 3294 4197 5074 5408 Size: 240 229 89 218 304 903 877 334 Cuts at: 5408 5547 5951 6075 7884 7969 9040 2214 Size: 139 404 124 1809 85 1071 2997 Fragments arranged by size: 2997 1809 1071 903 877 404 334 304 240 229 218 139 124 89 85 BsmI GAATG_Cn' Cuts at: 2739 4902 7334 7427 2739 Size: 2163 2432 93 5135 Fragments arranged by size: 5135 2432 2163 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4272 5312 5852 6703 6919 4272 Size: 1040 540 851 216 7176 Fragments arranged by size: 7176 1040 851 540 216 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2070 2186 2214 2295 2326 2386 Size: 399 271 9 116 28 81 31 60 Cuts at: 2386 2414 2454 2478 2683 2772 2851 2927 2990 Size: 28 40 24 205 89 79 76 63 Cuts at: 2990 3041 3048 3294 3397 3630 3688 4197 4514 Size: 51 7 246 103 233 58 509 317 Cuts at: 4514 5002 5074 5337 5408 5547 5731 5951 6075 Size: 488 72 263 71 139 184 220 124 Cuts at: 6075 6284 6501 6534 6821 6920 7074 7142 7884 Size: 209 217 33 287 99 154 68 742 Cuts at: 7884 7969 9040 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 509 488 399 317 287 271 263 246 233 220 217 209 205 184 154 139 124 116 103 99 89 85 81 79 76 72 71 68 63 60 58 51 40 33 31 28 28 24 9 7 BspEI T'CCGG_A Cuts at: 4048 9486 4048 Size: 5438 4385 BspHI T'CATG_A Cuts at: 5439 7712 9442 5439 Size: 2273 1730 5820 Fragments arranged by size: 5820 2273 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3244 5153 5325 5473 6266 6678 7178 844 Size: 2400 1909 172 148 793 412 500 3489 Fragments arranged by size: 3489 2400 1909 793 500 412 172 148 BsrBI CCG'CTC Cuts at: 1394 1823 7710 1394 Size: 429 5887 3507 Fragments arranged by size: 5887 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5768 6183 8318 8492 65 Size: 1183 4520 415 2135 174 1396 Fragments arranged by size: 4520 2135 1396 1183 415 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4458 4634 4726 6939 8467 8654 1700 Size: 110 2648 176 92 2213 1528 187 2869 Fragments arranged by size: 2869 2648 2213 1528 187 176 110 92 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9823 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9823 BssSI C'ACGA_G Cuts at: 4136 6566 7883 8895 4136 Size: 2430 1317 1012 5064 Fragments arranged by size: 5064 2430 1317 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2218 2696 2953 4161 5145 5767 5935 794 Size: 1424 478 257 1208 984 622 168 4682 Fragments arranged by size: 4682 1424 1208 984 622 478 257 168 BstBI TT'CG_AA Cuts at: 2007 8627 2007 Size: 6620 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9823 BstXI CCAn_nnnn'nTGG Cuts at: 2600 3637 4297 4402 4886 6838 2600 Size: 1037 660 105 484 1952 5585 Fragments arranged by size: 5585 1952 1037 660 484 105 BstYI r'GATC_y Cuts at: 1972 2139 2241 2616 3309 3564 3606 3931 4860 Size: 167 102 375 693 255 42 325 929 Cuts at: 4860 4917 5703 7153 7520 7905 7922 9374 9460 Size: 57 786 1450 367 385 17 1452 86 Cuts at: 9460 9472 9818 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1450 929 786 693 385 375 367 346 325 255 167 102 86 57 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2868 3456 3689 6275 7015 2868 Size: 588 233 2586 740 5676 Fragments arranged by size: 5676 2586 740 588 233 BtgI C'CryG_G Cuts at: 513 1065 2022 2919 4143 4251 5169 5275 5469 Size: 552 957 897 1224 108 918 106 194 Cuts at: 5469 6754 513 Size: 1285 3582 Fragments arranged by size: 3582 1285 1224 957 918 897 552 194 108 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2181 487 Size: 683 56 955 8129 Fragments arranged by size: 8129 955 683 56 BtsI GCAGTG_nn' Cuts at: 3149 3375 4118 6117 6368 7086 7339 8133 8153 Size: 226 743 1999 251 718 253 794 20 Cuts at: 8153 3149 Size: 4819 Fragments arranged by size: 4819 1999 794 743 718 253 251 226 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9823 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5794 5829 529 Size: 35 5230 35 4523 Fragments arranged by size: 5230 4523 35 35 DraI TTT'AAA Cuts at: 7127 7127 Size: 9823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2504 6785 8830 809 Size: 507 1188 4281 2045 1802 Fragments arranged by size: 4281 2045 1802 1188 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2188 2278 2916 Size: 54 1425 50 275 18 358 90 638 Cuts at: 2916 3372 3621 4943 5361 6691 7270 8159 9788 Size: 456 249 1322 418 1330 579 889 1629 Cuts at: 9788 8 Size: 43 Fragments arranged by size: 1629 1425 1330 1322 889 638 579 456 418 358 275 249 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7270 1537 Size: 275 5458 4090 Fragments arranged by size: 5458 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2777 3521 4773 4988 6084 6135 6547 6701 Size: 1424 744 1252 215 1096 51 412 154 Cuts at: 6701 7753 1353 Size: 1052 3423 Fragments arranged by size: 3423 1424 1252 1096 1052 744 412 215 154 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 5855 6140 8410 8784 8930 Size: 1064 138 501 3765 285 2270 374 146 Cuts at: 8930 387 Size: 1280 Fragments arranged by size: 3765 2270 1280 1064 501 374 285 146 138 EcoICRI GAG'CTC Cuts at: 2988 5406 2988 Size: 2418 7405 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2417 2505 2578 2918 2924 3058 Size: 393 731 54 88 73 340 6 134 Cuts at: 3058 3326 3433 3545 3554 3753 4112 4181 4340 Size: 268 107 112 9 199 359 69 159 Cuts at: 4340 4498 4847 5029 5261 5422 5660 5894 5909 Size: 158 349 182 232 161 238 234 15 Cuts at: 5909 5975 6041 6479 6821 7886 8483 9270 9538 Size: 66 66 438 342 1065 597 787 268 Cuts at: 9538 1239 Size: 1524 Fragments arranged by size: 1524 1065 787 731 597 438 393 359 349 342 340 268 268 238 234 232 199 182 161 159 158 134 112 107 88 73 69 66 66 54 15 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 3073 3076 4505 6274 6854 6911 3073 Size: 3 1429 1769 580 57 5985 Fragments arranged by size: 5985 1769 1429 580 57 3 EcoO109I rG'GnC_Cy Cuts at: 2066 2292 2322 2323 2497 2506 2604 2755 3694 Size: 226 30 1 174 9 98 151 939 Cuts at: 3694 3960 4062 4294 4925 5155 5422 6157 6531 Size: 266 102 232 631 230 267 735 374 Cuts at: 6531 6551 6592 6817 7019 2066 Size: 20 41 225 202 4870 Fragments arranged by size: 4870 939 735 631 374 267 266 232 230 226 225 202 174 151 102 98 41 30 20 9 1 EcoRI G'AATT_C Cuts at: 2048 7132 7510 2048 Size: 5084 378 4361 Fragments arranged by size: 5084 4361 378 FspI TGC'GCA Cuts at: 8329 8329 Size: 9823 HaeII r_GCGC'y Cuts at: 1678 2460 3412 4635 4876 5612 5971 8970 1678 Size: 782 952 1223 241 736 359 2999 2531 Fragments arranged by size: 2999 2531 1223 952 782 736 359 241 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 2351 2383 2596 Size: 32 135 32 596 32 151 32 213 Cuts at: 2596 2628 2954 2986 4269 4271 4301 4303 4398 Size: 32 326 32 1283 2 30 2 95 Cuts at: 4398 4430 5390 5422 6700 6732 6872 6904 8464 Size: 32 960 32 1278 32 140 32 1560 Cuts at: 8464 8496 8538 8570 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1560 1283 1278 960 596 326 213 151 140 135 95 42 32 32 32 32 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2894 3270 7167 7413 669 Size: 988 281 956 376 3897 246 3079 Fragments arranged by size: 3897 3079 988 956 376 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9823 HpaI GTT'AAC Cuts at: 7413 7413 Size: 9823 KpnI G_GTAC'C Cuts at: 7148 7148 Size: 9823 MfeI C'AATT_G Cuts at: 7422 7422 Size: 9823 MluI A'CGCG_T Cuts at: 9699 9699 Size: 9823 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4590 4647 6324 8937 9121 1305 Size: 3285 57 1677 2613 184 2007 Fragments arranged by size: 3285 2613 2007 1677 184 57 MscI TGG'CCA Cuts at: 10 64 1489 1832 2190 2280 2918 3374 4945 Size: 54 1425 343 358 90 638 456 1571 Cuts at: 4945 5363 10 Size: 418 4470 Fragments arranged by size: 4470 1571 1425 638 456 418 358 343 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 4418 5670 6759 7781 8140 8299 Size: 648 1359 1893 1252 1089 1022 359 159 Cuts at: 8299 518 Size: 2042 Fragments arranged by size: 2042 1893 1359 1252 1089 1022 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2395 2676 2821 3025 3053 3914 3997 4003 Size: 789 281 145 204 28 861 83 6 Cuts at: 4003 4099 4267 5277 5755 5998 7239 7916 9064 Size: 96 168 1010 478 243 1241 677 1148 Cuts at: 9064 9309 1606 Size: 245 2120 Fragments arranged by size: 2120 1241 1148 1010 861 789 677 478 281 245 243 204 168 145 96 83 28 6 NaeI GCC'GGC Cuts at: 4636 4728 4636 Size: 92 9731 NcoI C'CATG_G Cuts at: 513 1065 2022 2919 6754 513 Size: 552 957 897 3835 3582 Fragments arranged by size: 3835 3582 957 897 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9823 NgoMIV G'CCGG_C Cuts at: 4634 4726 4634 Size: 92 9731 NheI G'CTAG_C Cuts at: 1052 2686 1052 Size: 1634 8189 NotI GC'GGCC_GC Cuts at: 7270 7270 Size: 9823 NspI r_CATG'y Cuts at: 1132 1229 2081 2530 2801 3304 4370 4663 4726 Size: 97 852 449 271 503 1066 293 63 Cuts at: 4726 5330 6416 6514 6870 7181 8726 1132 Size: 604 1086 98 356 311 1545 2229 Fragments arranged by size: 2229 1545 1086 1066 852 604 503 449 356 311 293 271 98 97 63 PasI CC'CwG_GG Cuts at: 1348 2062 3041 5215 6831 1348 Size: 714 979 2174 1616 4340 Fragments arranged by size: 4340 2174 1616 979 714 PciI A'CATG_T Cuts at: 2526 8722 2526 Size: 6196 3627 PflMI CCAn_nnn'nTGG Cuts at: 1108 2110 2958 4941 5020 5134 5239 5737 5998 Size: 1002 848 1983 79 114 105 498 261 Cuts at: 5998 6700 1108 Size: 702 4231 Fragments arranged by size: 4231 1983 1002 848 702 498 261 114 105 79 PfoI T'CCnGG_A Cuts at: 2508 2811 3570 3927 4197 4824 4953 2508 Size: 303 759 357 270 627 129 7378 Fragments arranged by size: 7378 759 627 357 303 270 129 PmeI GTTT'AAAC Cuts at: 7127 7127 Size: 9823 PmlI CAC'GTG Cuts at: 2449 5668 2449 Size: 3219 6604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2891 2923 5171 5203 5555 5587 6281 6313 6995 Size: 32 2248 32 352 32 694 32 682 Cuts at: 6995 7027 7892 7924 9405 9437 2891 Size: 32 865 32 1481 32 3277 Fragments arranged by size: 3277 2248 1481 865 694 682 352 32 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2506 2604 2755 3694 3960 4062 6157 6592 Size: 9 98 151 939 266 102 2095 435 Cuts at: 6592 2497 Size: 5728 Fragments arranged by size: 5728 2095 939 435 266 151 102 98 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9823 PsiI TTA'TAA Cuts at: 7393 7393 Size: 9823 PspOMI G'GGCC_C Cuts at: 2066 2291 2322 6530 6817 2066 Size: 225 31 4208 287 5072 Fragments arranged by size: 5072 4208 287 225 31 PspXI vC'TCGA_Gb Cuts at: 4714 4714 Size: 9823 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3740 3772 3740 Size: 32 9791 PstI C_TGCA'G Cuts at: 830 2311 3156 3888 3996 4002 5562 5760 5967 Size: 1481 845 732 108 6 1560 198 207 Cuts at: 5967 6363 6393 7175 830 Size: 396 30 782 3478 Fragments arranged by size: 3478 1560 1481 845 782 732 396 207 198 108 30 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9823 PvuII CAG'CTG Cuts at: 2395 2821 3025 3914 3997 4099 4267 5755 5998 Size: 426 204 889 83 102 168 1488 243 Cuts at: 5998 2395 Size: 6220 Fragments arranged by size: 6220 1488 889 426 243 204 168 102 83 RsrII CG'GwC_CG Cuts at: 5842 5842 Size: 9823 SacI G_AGCT'C Cuts at: 2990 5408 2990 Size: 2418 7405 SacII CC_GC'GG Cuts at: 5278 5278 Size: 9823 SalI G'TCGA_C Cuts at: 1936 7165 1936 Size: 5229 4594 SanDI GG'GwC_CC Cuts at: 2604 3960 2604 Size: 1356 8467 SapI GCTCTTCn'nnn_ Cuts at: 2777 2777 Size: 9823 SbfI CC_TGCA'GG Cuts at: 7175 7175 Size: 9823 ScaI AGT'ACT Cuts at: 8071 9783 8071 Size: 1712 8111 SfcI C'TryA_G Cuts at: 826 1045 2041 2307 3152 3884 3992 3998 5558 Size: 219 996 266 845 732 108 6 1560 Cuts at: 5558 5756 5963 6359 6389 7049 7171 8306 8987 Size: 198 207 396 30 660 122 1135 681 Cuts at: 8987 9178 826 Size: 191 1471 Fragments arranged by size: 1560 1471 1135 996 845 732 681 660 396 266 219 207 198 191 122 108 30 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9823 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9823 SmaI CCC'GGG Cuts at: 1872 5420 7064 7150 1872 Size: 3548 1644 86 4545 Fragments arranged by size: 4545 3548 1644 86 SmlI C'TyrA_G Cuts at: 820 1017 1943 4714 5566 5863 6335 6596 6746 Size: 197 926 2771 852 297 472 261 150 Cuts at: 6746 7927 8828 9090 820 Size: 1181 901 262 1553 Fragments arranged by size: 2771 1553 1181 926 901 852 472 297 262 261 197 150 SnaBI TAC'GTA Cuts at: 493 7120 7614 493 Size: 6627 494 2702 Fragments arranged by size: 6627 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9823 SphI G_CATG'C Cuts at: 1132 4370 6870 7181 1132 Size: 3238 2500 311 3774 Fragments arranged by size: 3774 3238 2500 311 SspI AAT'ATT Cuts at: 5 52 5554 7747 5 Size: 47 5502 2193 2081 Fragments arranged by size: 5502 2193 2081 47 StuI AGG'CCT Cuts at: 1517 2969 5006 1517 Size: 1452 2037 6334 Fragments arranged by size: 6334 2037 1452 StyI C'CwwG_G Cuts at: 513 1065 2022 2232 2501 2919 2981 3361 4290 Size: 552 957 210 269 418 62 380 929 Cuts at: 4290 4921 5409 6052 6754 6850 7262 513 Size: 631 488 643 702 96 412 3074 Fragments arranged by size: 3074 957 929 702 643 631 552 488 418 412 380 269 210 96 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3277 5888 7109 7850 7867 8020 8205 Size: 1255 1697 2611 1221 741 17 153 185 Cuts at: 8205 8650 325 Size: 445 1498 Fragments arranged by size: 2611 1697 1498 1255 1221 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 8069 9781 96 Size: 274 80 33 51 1443 6092 1712 138 Fragments arranged by size: 6092 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2404 2436 2848 2880 4110 4142 4523 4555 6980 Size: 32 412 32 1230 32 381 32 2425 Cuts at: 6980 7012 9783 9815 2404 Size: 32 2771 32 2412 Fragments arranged by size: 2771 2425 2412 1230 412 381 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9823 XbaI T'CTAG_A Cuts at: 3769 3934 5057 7159 3769 Size: 165 1123 2102 6433 Fragments arranged by size: 6433 2102 1123 165 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3382 3382 Size: 9823 XhoI C'TCGA_G Cuts at: 1943 4714 5863 1943 Size: 2771 1149 5903 Fragments arranged by size: 5903 2771 1149 XmaI C'CCGG_G Cuts at: 1870 5418 7062 7148 1870 Size: 3548 1644 86 4545 Fragments arranged by size: 4545 3548 1644 86 XmnI GAAnn'nnTTC Cuts at: 7952 7952 Size: 9823 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6883 7631 276 Size: 53 83 186 936 5349 748 2468 Fragments arranged by size: 5349 2468 936 748 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI SexAI SfiI SfoI SrfI SwaI