(Circular) (Six-base) MAPSORT of: pFN21ASDA0074.seq Check: 927 from: 1 to: 6952 pFN21ASDA0074 6952 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4762 278 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2550 3042 4295 1937 Size: 613 492 1253 4594 Fragments arranged by size: 4594 1253 613 492 Acc65I G'GTAC_C Cuts at: 4273 4273 Size: 6952 AclI AA'CG_TT Cuts at: 1563 5079 5452 1563 Size: 3516 373 3063 Fragments arranged by size: 3516 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2055 2094 3105 3983 5015 6399 6667 1632 Size: 423 39 1011 878 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1032 1011 878 423 268 39 AfeI AGC'GCT Cuts at: 3993 3993 Size: 6952 AflII C'TTAA_G Cuts at: 820 1017 3614 820 Size: 197 2597 4158 Fragments arranged by size: 4158 2597 197 AflIII A'CryG_T Cuts at: 1203 5851 6828 1203 Size: 4648 977 1327 Fragments arranged by size: 4648 1327 977 AgeI A'CCGG_T Cuts at: 5783 5783 Size: 6952 AhdI GACnn_n'nnGTC Cuts at: 5681 5681 Size: 6952 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2964 1184 Size: 1780 5172 AlwNI CAG_nnn'CTG Cuts at: 3198 4695 6267 3198 Size: 1497 1572 3883 Fragments arranged by size: 3883 1572 1497 ApaLI G'TGCA_C Cuts at: 5009 6165 5009 Size: 1156 5796 ApoI r'AATT_y Cuts at: 1493 3918 4261 4487 4639 6748 1493 Size: 2425 343 226 152 2109 1697 Fragments arranged by size: 2425 2109 1697 343 226 152 AseI AT'TA_AT Cuts at: 160 1032 5506 160 Size: 872 4474 1606 Fragments arranged by size: 4474 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 AvaI C'yCGr_G Cuts at: 1870 1943 2230 2888 4277 1870 Size: 73 287 658 1389 4545 Fragments arranged by size: 4545 1389 658 287 73 BamHI G'GATC_C Cuts at: 4282 4649 4282 Size: 367 6585 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2165 3602 4273 5728 Size: 325 217 627 359 19 1437 671 1455 Cuts at: 5728 618 Size: 1842 Fragments arranged by size: 1842 1455 1437 671 627 359 325 217 19 BanII G_rGCy'C Cuts at: 1391 3210 4092 4271 1391 Size: 1819 882 179 4072 Fragments arranged by size: 4072 1819 882 179 BbeI G_GCGC'C Cuts at: 2150 2150 Size: 6952 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2187 2768 3672 928 Size: 971 288 581 904 4208 Fragments arranged by size: 4208 971 904 581 288 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2116 2150 4084 4118 5143 5177 1903 Size: 34 179 34 1934 34 1025 34 3678 Fragments arranged by size: 3678 1934 1025 179 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4844 6054 4844 Size: 1210 5742 BclI T'GATC_A Cuts at: 1548 1548 Size: 6952 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5563 136 Size: 107 122 71 631 605 3891 1525 Fragments arranged by size: 3891 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2511 2964 6947 2511 Size: 453 3983 2516 Fragments arranged by size: 3983 2516 453 BlpI GC'TnA_GC Cuts at: 3891 4082 4369 3891 Size: 191 287 6474 Fragments arranged by size: 6474 287 191 Bme1580I G_kGCm'C Cuts at: 1790 3278 5013 6169 1790 Size: 1488 1735 1156 2573 Fragments arranged by size: 2573 1735 1488 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6952 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2165 4683 5641 Size: 416 53 83 399 24 748 2518 958 Cuts at: 5641 442 Size: 1753 Fragments arranged by size: 2518 1753 958 748 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6952 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2903 4234 2903 Size: 1331 5621 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2272 3318 3822 5612 1239 Size: 1033 1046 504 1790 2579 Fragments arranged by size: 2579 1790 1046 1033 504 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5077 5942 6240 5077 Size: 865 298 5789 Fragments arranged by size: 5789 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5615 882 Size: 617 4116 2219 Fragments arranged by size: 4116 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4249 4743 493 Size: 711 3045 494 2702 Fragments arranged by size: 3045 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6946 6946 Size: 6952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 4759 5141 5776 Size: 53 83 186 936 614 2612 382 635 Cuts at: 5776 275 Size: 1451 Fragments arranged by size: 2612 1451 936 635 614 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3998 5385 5783 6057 6204 6615 3998 Size: 1387 398 274 147 411 4335 Fragments arranged by size: 4335 1387 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 3680 3710 4233 Size: 30 165 30 681 30 1566 30 523 Cuts at: 4233 4263 1178 Size: 30 3867 Fragments arranged by size: 3867 1566 681 523 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 4123 1186 Size: 906 2031 4015 Fragments arranged by size: 4015 2031 906 BseYI C'CCAG_C Cuts at: 2038 2569 6155 6651 2038 Size: 531 3586 496 2339 Fragments arranged by size: 3586 2339 531 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2806 2970 1163 Size: 573 1070 164 5145 Fragments arranged by size: 5145 1070 573 164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4402 5163 6191 1540 Size: 275 186 2401 761 1028 2301 Fragments arranged by size: 2401 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5013 5098 6169 5013 Size: 85 1071 5796 Fragments arranged by size: 5796 1071 85 BsmI GAATG_Cn' Cuts at: 2407 4463 4556 2407 Size: 2056 93 4803 Fragments arranged by size: 4803 2056 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2069 2069 Size: 6952 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3210 3278 4092 4271 5013 5098 6169 Size: 399 1420 68 814 179 742 85 1071 Cuts at: 6169 1391 Size: 2174 Fragments arranged by size: 2174 1420 1071 814 742 399 179 85 68 BspEI T'CCGG_A Cuts at: 3998 6615 3998 Size: 2617 4335 BspHI T'CATG_A Cuts at: 3181 4841 6571 3181 Size: 1660 1730 3562 Fragments arranged by size: 3562 1730 1660 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4110 4307 844 Size: 3266 197 3489 Fragments arranged by size: 3489 3266 197 BsrBI CCG'CTC Cuts at: 1394 1823 2212 4839 1394 Size: 429 389 2627 3507 Fragments arranged by size: 3507 2627 429 389 BsrDI GCAATG_nn' Cuts at: 65 1248 5447 5621 65 Size: 1183 4199 174 1396 Fragments arranged by size: 4199 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5596 5783 1700 Size: 110 3786 187 2869 Fragments arranged by size: 3786 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6952 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6952 BssSI C'ACGA_G Cuts at: 5012 6024 5012 Size: 1012 5940 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6952 BstBI TT'CG_AA Cuts at: 2007 5756 2007 Size: 3749 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6952 BstXI CCAn_nnnn'nTGG Cuts at: 3946 4150 3946 Size: 204 6748 BstYI r'GATC_y Cuts at: 1972 2511 2964 4282 4649 5034 5051 6503 6589 Size: 539 453 1318 367 385 17 1452 86 Cuts at: 6589 6601 6947 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1318 539 453 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2551 2551 Size: 6952 BtgI C'CryG_G Cuts at: 513 1065 2022 2874 513 Size: 552 957 852 4591 Fragments arranged by size: 4591 957 852 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6213 Fragments arranged by size: 6213 683 56 BtsI GCAGTG_nn' Cuts at: 2042 2818 2951 4468 5262 5282 2042 Size: 776 133 1517 794 20 3712 Fragments arranged by size: 3712 1517 794 776 133 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6917 DraI TTT'AAA Cuts at: 2476 2928 4256 2476 Size: 452 1328 5172 Fragments arranged by size: 5172 1328 452 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3578 5959 809 Size: 507 2262 2381 1802 Fragments arranged by size: 2381 2262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2979 4399 5288 Size: 54 1425 50 275 18 1149 1420 889 Cuts at: 5288 6917 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1420 1149 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4399 1537 Size: 275 2587 4090 Fragments arranged by size: 4090 2587 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2071 3180 3407 4882 1353 Size: 718 1109 227 1475 3423 Fragments arranged by size: 3423 1475 1109 718 227 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5539 5913 6059 387 Size: 1064 138 3950 374 146 1280 Fragments arranged by size: 3950 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2094 2272 3105 3318 3822 3983 Size: 393 423 39 178 833 213 504 161 Cuts at: 3983 5015 5612 6399 6667 1239 Size: 1032 597 787 268 1524 Fragments arranged by size: 1524 1032 833 787 597 504 423 393 268 213 178 161 39 EcoNI CCTnn'n_nnAGG Cuts at: 2290 2290 Size: 6952 EcoO109I rG'GnC_Cy Cuts at: 2027 3123 3764 4099 4213 2027 Size: 1096 641 335 114 4766 Fragments arranged by size: 4766 1096 641 335 114 EcoRI G'AATT_C Cuts at: 4261 4639 4261 Size: 378 6574 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2692 2692 Size: 6952 FspI TGC'GCA Cuts at: 5458 5458 Size: 6952 HaeII r_GCGC'y Cuts at: 1678 2150 2230 2936 3361 3995 6099 1678 Size: 472 80 706 425 634 2104 2531 Fragments arranged by size: 2531 2104 706 634 472 425 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 2570 2602 3014 Size: 32 135 32 845 32 121 32 412 Cuts at: 3014 3046 3843 3875 5593 5625 5667 5699 1373 Size: 32 797 32 1718 32 42 32 2626 Fragments arranged by size: 2626 1718 845 797 412 135 121 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3091 3571 4296 4542 669 Size: 988 281 1153 480 725 246 3079 Fragments arranged by size: 3079 1153 988 725 480 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6952 HpaI GTT'AAC Cuts at: 4542 4542 Size: 6952 KasI G'GCGC_C Cuts at: 2146 2146 Size: 6952 KpnI G_GTAC'C Cuts at: 4277 4277 Size: 6952 MfeI C'AATT_G Cuts at: 4551 4551 Size: 6952 MluI A'CGCG_T Cuts at: 6828 6828 Size: 6952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3723 6066 6250 1305 Size: 2418 2343 184 2007 Fragments arranged by size: 2418 2343 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5130 Fragments arranged by size: 5130 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2544 3332 4910 5269 5428 518 Size: 648 1378 788 1578 359 159 2042 Fragments arranged by size: 2042 1578 1378 788 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2221 4368 5045 6193 6438 1606 Size: 615 2147 677 1148 245 2120 Fragments arranged by size: 2147 2120 1148 677 615 245 NarI GG'CG_CC Cuts at: 2147 2147 Size: 6952 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5443 Fragments arranged by size: 5443 957 552 NdeI CA'TA_TG Cuts at: 387 3868 387 Size: 3481 3471 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6952 NotI GC'GGCC_GC Cuts at: 4399 4399 Size: 6952 NspI r_CATG'y Cuts at: 1132 1229 3976 4310 5855 1132 Size: 97 2747 334 1545 2229 Fragments arranged by size: 2747 2229 1545 334 97 PasI CC'CwG_GG Cuts at: 1348 3127 3625 1348 Size: 1779 498 4675 Fragments arranged by size: 4675 1779 498 PciI A'CATG_T Cuts at: 5851 5851 Size: 6952 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6952 PmeI GTTT'AAAC Cuts at: 4256 4256 Size: 6952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2105 2137 5021 5053 6534 6566 2105 Size: 32 2884 32 1481 32 2491 Fragments arranged by size: 2884 2491 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2027 3123 3764 4099 4213 2027 Size: 1096 641 335 114 4766 Fragments arranged by size: 4766 1096 641 335 114 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6952 PsiI TTA'TAA Cuts at: 4522 4522 Size: 6952 PspXI vC'TCGA_Gb Cuts at: 2230 2230 Size: 6952 PstI C_TGCA'G Cuts at: 830 2220 2250 4107 4304 830 Size: 1390 30 1857 197 3478 Fragments arranged by size: 3478 1857 1390 197 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6952 SalI G'TCGA_C Cuts at: 1936 4294 1936 Size: 2358 4594 SanDI GG'GwC_CC Cuts at: 3123 3123 Size: 6952 SbfI CC_TGCA'GG Cuts at: 4304 4304 Size: 6952 ScaI AGT'ACT Cuts at: 5200 6912 5200 Size: 1712 5240 SexAI A'CCwGG_T Cuts at: 3063 3605 3063 Size: 542 6410 SfcI C'TryA_G Cuts at: 826 1045 2216 2246 2830 3549 4103 4300 5435 Size: 219 1171 30 584 719 554 197 1135 Cuts at: 5435 6116 6307 826 Size: 681 191 1471 Fragments arranged by size: 1471 1171 1135 719 681 584 554 219 197 191 30 SfoI GGC'GCC Cuts at: 2148 2148 Size: 6952 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6952 SmaI CCC'GGG Cuts at: 1872 4279 1872 Size: 2407 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2230 2888 3614 5056 5957 6219 Size: 197 926 287 658 726 1442 901 262 Cuts at: 6219 820 Size: 1553 Fragments arranged by size: 1553 1442 926 901 726 658 287 262 197 SnaBI TAC'GTA Cuts at: 493 4249 4743 493 Size: 3756 494 2702 Fragments arranged by size: 3756 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6952 SphI G_CATG'C Cuts at: 1132 4310 1132 Size: 3178 3774 SspI AAT'ATT Cuts at: 5 52 2158 3305 4876 5 Size: 47 2106 1147 1571 2081 Fragments arranged by size: 2106 2081 1571 1147 47 StuI AGG'CCT Cuts at: 1517 3367 1517 Size: 1850 5102 StyI C'CwwG_G Cuts at: 513 1065 2022 2560 3247 3847 4391 513 Size: 552 957 538 687 600 544 3074 Fragments arranged by size: 3074 957 687 600 552 544 538 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4979 4996 5149 5334 5779 325 Size: 1255 3399 17 153 185 445 1498 Fragments arranged by size: 3399 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3416 5198 6910 Size: 274 80 33 51 1443 1439 1782 1712 Cuts at: 6910 96 Size: 138 Fragments arranged by size: 1782 1712 1443 1439 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2084 2116 6912 6944 2084 Size: 32 4796 32 2092 Fragments arranged by size: 4796 2092 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3328 3505 1316 Size: 2012 177 4763 Fragments arranged by size: 4763 2012 177 XbaI T'CTAG_A Cuts at: 4288 4288 Size: 6952 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2615 3170 3511 4034 2476 Size: 139 555 341 523 5394 Fragments arranged by size: 5394 555 523 341 139 XhoI C'TCGA_G Cuts at: 1943 2230 2888 1943 Size: 287 658 6007 Fragments arranged by size: 6007 658 287 XmaI C'CCGG_G Cuts at: 1870 4277 1870 Size: 2407 4545 XmnI GAAnn'nnTTC Cuts at: 2184 5081 2184 Size: 2897 4055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4760 276 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacI SacII SapI SfiI SrfI SwaI