Restriction map for
pFN21ASDA0074
(Linear) (Six-base) MAPSORT of: KIAA0074.seq Check: 9772 from: 1 to: 2223
KIAA0074.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 527 1019 2223
Size: 527 492 1204
Fragments arranged by size:
1204 527 492
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 71 1082 1960 2223
Size: 32 39 1011 878 263
Fragments arranged by size:
1011 878 263 39 32
AfeI AGC'GCT
Cuts at: 0 1970 2223
Size: 1970 253
AflII C'TTAA_G
Cuts at: 0 1591 2223
Size: 1591 632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 941 2223
Size: 941 1282
AlwNI CAG_nnn'CTG
Cuts at: 0 1175 2223
Size: 1175 1048
ApoI r'AATT_y
Cuts at: 0 1895 2223
Size: 1895 328
AvaI C'yCGr_G
Cuts at: 0 207 865 2223
Size: 207 658 1358
Fragments arranged by size:
1358 658 207
BanI G'GyrC_C
Cuts at: 0 123 142 1579 2223
Size: 123 19 1437 644
Fragments arranged by size:
1437 644 123 19
BanII G_rGCy'C
Cuts at: 0 1187 2069 2223
Size: 1187 882 154
Fragments arranged by size:
1187 882 154
BbeI G_GCGC'C
Cuts at: 0 127 2223
Size: 127 2096
BbsI GAAGACnn'nnnn_
Cuts at: 0 164 745 1649 2223
Size: 164 581 904 574
Fragments arranged by size:
904 581 574 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 2061 2095 2223
Size: 93 34 1934 34 128
Fragments arranged by size:
1934 128 93 34 34
BglII A'GATC_T
Cuts at: 0 488 941 2223
Size: 488 453 1282
Fragments arranged by size:
1282 488 453
BlpI GC'TnA_GC
Cuts at: 0 1868 2059 2223
Size: 1868 191 164
Fragments arranged by size:
1868 191 164
Bme1580I G_kGCm'C
Cuts at: 0 1255 2223
Size: 1255 968
BmrI ACTGGGnnnn_n'
Cuts at: 0 142 2223
Size: 142 2081
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 880 2211 2223
Size: 880 1331 12
Fragments arranged by size:
1331 880 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 1295 1799 2223
Size: 249 1046 504 424
Fragments arranged by size:
1046 504 424 249
BsaHI Gr'CG_yC
Cuts at: 0 124 2223
Size: 124 2099
BsaWI w'CCGG_w
Cuts at: 0 1975 2223
Size: 1975 248
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 1657 1687 2223
Size: 61 30 1566 30 536
Fragments arranged by size:
1566 536 61 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 2100 2223
Size: 69 2031 123
Fragments arranged by size:
2031 123 69
BseYI C'CCAG_C
Cuts at: 0 15 546 2223
Size: 15 531 1677
Fragments arranged by size:
1677 531 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 947 2223
Size: 783 164 1276
Fragments arranged by size:
1276 783 164
BsmI GAATG_Cn'
Cuts at: 0 384 2223
Size: 384 1839
BsmBI CGTCTCn'nnnn_
Cuts at: 0 46 2223
Size: 46 2177
Bsp1286I G_dGCh'C
Cuts at: 0 1187 1255 2069 2223
Size: 1187 68 814 154
Fragments arranged by size:
1187 814 154 68
BspEI T'CCGG_A
Cuts at: 0 1975 2223
Size: 1975 248
BspHI T'CATG_A
Cuts at: 0 1158 2223
Size: 1158 1065
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2087 2223
Size: 2087 136
BsrBI CCG'CTC
Cuts at: 0 189 2223
Size: 189 2034
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1923 2127 2223
Size: 1923 204 96
Fragments arranged by size:
1923 204 96
BstYI r'GATC_y
Cuts at: 0 488 941 2223
Size: 488 453 1282
Fragments arranged by size:
1282 488 453
BstZ17I GTA'TAC
Cuts at: 0 528 2223
Size: 528 1695
BtgI C'CryG_G
Cuts at: 0 851 2223
Size: 851 1372
BtsI GCAGTG_nn'
Cuts at: 0 19 795 928 2223
Size: 19 776 133 1295
Fragments arranged by size:
1295 776 133 19
DraI TTT'AAA
Cuts at: 0 453 905 2223
Size: 453 452 1318
Fragments arranged by size:
1318 453 452
DrdI GACnn_nn'nnGTC
Cuts at: 0 1555 2223
Size: 1555 668
EaeI y'GGCC_r
Cuts at: 0 956 2223
Size: 956 1267
EarI CTCTTCn'nnn_
Cuts at: 0 48 1157 1384 2223
Size: 48 1109 227 839
Fragments arranged by size:
1109 839 227 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 71 249 1082 1295 1799 1960 2223
Size: 32 39 178 833 213 504 161 263
Fragments arranged by size:
833 504 263 213 178 161 39 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 267 2223
Size: 267 1956
EcoO109I rG'GnC_Cy
Cuts at: 0 4 1100 1741 2076 2190 2223
Size: 4 1096 641 335 114 33
Fragments arranged by size:
1096 641 335 114 33 4
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 669 2223
Size: 669 1554
HaeII r_GCGC'y
Cuts at: 0 127 207 913 1338 1972 2223
Size: 127 80 706 425 634 251
Fragments arranged by size:
706 634 425 251 127 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 547 579 991 1023 1820 1852
Size: 394 32 121 32 412 32 797 32
Cuts at: 1852 2223
Size: 371
Fragments arranged by size:
797 412 394 371 121 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1068 1548 2223
Size: 1068 480 675
Fragments arranged by size:
1068 675 480
KasI G'GCGC_C
Cuts at: 0 123 2223
Size: 123 2100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1700 2223
Size: 1700 523
MslI CAynn'nnrTG
Cuts at: 0 521 1309 2223
Size: 521 788 914
Fragments arranged by size:
914 788 521
MspA1I CmG'CkG
Cuts at: 0 198 2223
Size: 198 2025
NarI GG'CG_CC
Cuts at: 0 124 2223
Size: 124 2099
NdeI CA'TA_TG
Cuts at: 0 1845 2223
Size: 1845 378
NspI r_CATG'y
Cuts at: 0 1953 2223
Size: 1953 270
PasI CC'CwG_GG
Cuts at: 0 1104 1602 2223
Size: 1104 498 621
Fragments arranged by size:
1104 621 498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 2223
Size: 82 32 2109
Fragments arranged by size:
2109 82 32
PpuMI rG'GwC_Cy
Cuts at: 0 4 1100 1741 2076 2190 2223
Size: 4 1096 641 335 114 33
Fragments arranged by size:
1096 641 335 114 33 4
PspXI vC'TCGA_Gb
Cuts at: 0 207 2223
Size: 207 2016
PstI C_TGCA'G
Cuts at: 0 197 227 2084 2223
Size: 197 30 1857 139
Fragments arranged by size:
1857 197 139 30
SanDI GG'GwC_CC
Cuts at: 0 1100 2223
Size: 1100 1123
SexAI A'CCwGG_T
Cuts at: 0 1040 1582 2223
Size: 1040 542 641
Fragments arranged by size:
1040 641 542
SfcI C'TryA_G
Cuts at: 0 193 223 807 1526 2080 2223
Size: 193 30 584 719 554 143
Fragments arranged by size:
719 584 554 193 143 30
SfoI GGC'GCC
Cuts at: 0 125 2223
Size: 125 2098
SmlI C'TyrA_G
Cuts at: 0 207 865 1591 2223
Size: 207 658 726 632
Fragments arranged by size:
726 658 632 207
SspI AAT'ATT
Cuts at: 0 135 1282 2223
Size: 135 1147 941
Fragments arranged by size:
1147 941 135
StuI AGG'CCT
Cuts at: 0 1344 2223
Size: 1344 879
StyI C'CwwG_G
Cuts at: 0 537 1224 1824 2223
Size: 537 687 600 399
Fragments arranged by size:
687 600 537 399
TatI w'GTAC_w
Cuts at: 0 1393 2223
Size: 1393 830
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 2223
Size: 61 32 2130
Fragments arranged by size:
2130 61 32
Tth111I GACn'n_nGTC
Cuts at: 0 1305 1482 2223
Size: 1305 177 741
Fragments arranged by size:
1305 741 177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 592 1147 1488 2011 2223
Size: 453 139 555 341 523 212
Fragments arranged by size:
555 523 453 341 212 139
XhoI C'TCGA_G
Cuts at: 0 207 865 2223
Size: 207 658 1358
Fragments arranged by size:
1358 658 207
XmnI GAAnn'nnTTC
Cuts at: 0 161 2223
Size: 161 2062
Enzymes that do cut:
AccI AcuI AfeI AflII AlfI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BcgI BglII BlpI Bme1580I
BmrI BplI BpmI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BstXI BstYI BstZ17I BtgI BtsI DraI DrdI EaeI
EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
KasI MmeI MslI MspA1I NarI NdeI NspI PasI
PpiI PpuMI PspXI PstI SanDI SexAI SfcI SfoI
SmlI SspI StuI StyI TatI TstI Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglI BmgBI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsiEI BsiHKAI BsiWI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
Bsu36I BtgZI ClaI CspCI DraIII EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI NaeI NcoI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII XbaI
XmaI ZraI