Restriction map for
pFN21ASDA0069
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AlwI 187 GATCC GGATC(4/5)
AlwNI 41 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 290 GGACC G^G[AT]CC
BalI 557 TGGCCA TGG^CCA
BsmAI 43 GAGAC GTCTC(1/5)
BsmFI 453 GGGAC GGGAC(10/14)
BspHI 413 TCATGA T^CATGA
BstAPI 40 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 73 GGATG GGATG(2/0)
BstXI 26 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 186 AGATCC [GA]^GATC[CT]
Cac8I 33 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 262 GCGC GCG^C
Cfr13I 290 GGACC G^G[ACGT]CC
DdeI 401 CTAAG C^T[ACGT]AG
EaeI 557 TGGCCA [CT]^GGCC[GA]
EarI 376 CTCTTC CTCTTC(1/4)
FokI 73 GGATG GGATG(9/13)
HaeII 261 AGCGCC [GA]GCGC^[CT]
HaeIII 558 GGCC GG^CC
HapII 203 CCGG C^CGG
HgaI 347 GACGC GACGC(5/10)
HhaI 262 GCGC GCG^C
HinP1I 262 GCGC G^CGC
HpaII 203 CCGG C^CGG
HphI 479 TCACC GGTGA(8/7)
Ksp632I 376 CTCTTC CTCTTC(1/4)
MaeIII 493 GTGAC ^GT[ACGT]AC
MflI 186 AGATCC [GA]^GATC[CT]
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
MspA1I 64 CAGCTG C[AC]G^C[GT]G
MspI 203 CCGG C^CGG
NlaIV 277 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 281 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 38 CTGCAG CTGCA^G
PvuII 64 CAGCTG CAG^CTG
RcaI 413 TCATGA T^CATGA
Sau96I 290 GGACC G^G[ACGT]CC
SfaNI 87 GATGC GCATC(5/9)
SfcI 38 CTGCAG C^T[GA][CT]AG
TaqI 345 TCGA T^CGA
Tsp45I 493 GTGAC ^GT[GC]AC
TspRI 294 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 345 TCGA T^CGA
Van91I 281 CCAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 183 TCTAGA T^CTAGA
XcmI 140 CCATCATGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 186 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
439 GCGG
AcsI 269 GAATTT [GA]^AATT[CT]
320 AAATTT
AfaI 408 GTAC GT^AC
546 GTAC
AhdI 45 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
455 GACAACAAGTC
AluI 65 AGCT AG^CT
354 AGCT
ApoI 269 GAATTT [GA]^AATT[CT]
320 AAATTT
AspEI 45 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
455 GACAACAAGTC
BssKI 125 CCTGG ^CC[ACGT]GG
514 CCTGG
BstNI 125 CCTGG CC^[AT]GG
514 CCTGG
DpnII 187 GATC ^GATC
423 GATC
Eam1105I 45 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
455 GACAACAAGTC
EcoRII 125 CCTGG ^CC[AT]GG
514 CCTGG
HinfI 311 GATTC G^A[ACGT]TC
533 GAATC
MboI 187 GATC ^GATC
423 GATC
MvaI 125 CCTGG CC^[AT]GG
514 CCTGG
MwoI 40 GCAGAGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
436 GCTGCGGTTGC
NdeII 187 GATC ^GATC
423 GATC
RsaI 408 GTAC GT^AC
546 GTAC
Sau3AI 187 GATC ^GATC
423 GATC
ScrFI 125 CCTGG CC^[ACGT]GG
514 CCTGG
TfiI 311 GATTC G^A[AT]TC
533 GAATC
Tsp509I 270 AATT ^AATT
321 AATT
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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BbvI BfaI BsiYI BslI BspMI
Fnu4HI ItaI MaeI MboII MnlI
MslI NlaIII TseI