(Circular) (Six-base) MAPSORT of: pFN21ASDA0067.seq Check: 5774 from: 1 to: 8602 pFN21ASDA0067 8602 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3400 3418 3425 3400 Size: 18 7 8577 Fragments arranged by size: 8577 18 7 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5890 6412 278 Size: 53 83 186 936 4354 522 2468 Fragments arranged by size: 4354 2468 936 522 186 83 53 AccI GT'mk_AC Cuts at: 1937 2743 4342 4671 5945 1937 Size: 806 1599 329 1274 4594 Fragments arranged by size: 4594 1599 1274 806 329 Acc65I G'GTAC_C Cuts at: 5923 5923 Size: 8602 AclI AA'CG_TT Cuts at: 1563 6729 7102 1563 Size: 5166 373 3063 Fragments arranged by size: 5166 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2100 4737 4753 5253 5463 6665 8049 8317 Size: 468 2637 16 500 210 1202 1384 268 Cuts at: 8317 1632 Size: 1917 Fragments arranged by size: 2637 1917 1384 1202 500 468 268 210 16 AflII C'TTAA_G Cuts at: 820 1017 2491 820 Size: 197 1474 6931 Fragments arranged by size: 6931 1474 197 AflIII A'CryG_T Cuts at: 1203 3275 4392 7501 8478 1203 Size: 2072 1117 3109 977 1327 Fragments arranged by size: 3109 2072 1327 1117 977 AgeI A'CCGG_T Cuts at: 3338 4404 7433 3338 Size: 1066 3029 4507 Fragments arranged by size: 4507 3029 1066 AhdI GACnn_n'nnGTC Cuts at: 7331 7331 Size: 8602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8602 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3200 3232 4413 4445 3200 Size: 32 1181 32 7357 Fragments arranged by size: 7357 1181 32 32 AlwNI CAG_nnn'CTG Cuts at: 2560 3242 3334 3515 3626 4190 6345 7917 2560 Size: 682 92 181 111 564 2155 1572 3245 Fragments arranged by size: 3245 2155 1572 682 564 181 111 92 ApaI G_GGCC'C Cuts at: 3697 3697 Size: 8602 ApaLI G'TGCA_C Cuts at: 4396 6659 7815 4396 Size: 2263 1156 5183 Fragments arranged by size: 5183 2263 1156 ApoI r'AATT_y Cuts at: 1493 2631 2720 4193 5911 6137 6289 8398 1493 Size: 1138 89 1473 1718 226 152 2109 1697 Fragments arranged by size: 2109 1718 1697 1473 1138 226 152 89 AseI AT'TA_AT Cuts at: 160 1032 7156 160 Size: 872 6124 1606 Fragments arranged by size: 6124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8602 AvaI C'yCGr_G Cuts at: 1870 1943 3117 3774 4159 4815 5514 5927 1870 Size: 73 1174 657 385 656 699 413 4545 Fragments arranged by size: 4545 1174 699 657 656 413 385 73 BamHI G'GATC_C Cuts at: 3150 4003 5932 6299 3150 Size: 853 1929 367 5453 Fragments arranged by size: 5453 1929 853 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2669 5923 7378 618 Size: 325 217 627 882 3254 1455 1842 Fragments arranged by size: 3254 1842 1455 882 627 325 217 BanII G_rGCy'C Cuts at: 1391 3213 3524 3697 3708 5921 1391 Size: 1822 311 173 11 2213 4072 Fragments arranged by size: 4072 2213 1822 311 173 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 3003 3664 5287 5719 5882 928 Size: 971 120 984 661 1623 432 163 3648 Fragments arranged by size: 3648 1623 984 971 661 432 163 120 BbvCI CC'TCA_GC Cuts at: 3235 3640 3235 Size: 405 8197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6793 6827 1903 Size: 34 4856 34 3678 Fragments arranged by size: 4856 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2964 4166 4481 5728 6494 7704 2964 Size: 1202 315 1247 766 1210 3862 Fragments arranged by size: 3862 1247 1210 1202 766 315 BclI T'GATC_A Cuts at: 1548 1548 Size: 8602 BfrBI ATG'CAT Cuts at: 2276 5269 2276 Size: 2993 5609 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7213 136 Size: 107 122 71 631 605 5541 1525 Fragments arranged by size: 5541 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4873 8597 4873 Size: 3724 4878 BlpI GC'TnA_GC Cuts at: 6019 6019 Size: 8602 Bme1580I G_kGCm'C Cuts at: 1790 3697 4400 6663 7819 1790 Size: 1907 703 2263 1156 2573 Fragments arranged by size: 2573 2263 1907 1156 703 BmgBI CAC'GTC Cuts at: 1318 3278 3498 1318 Size: 1960 220 6422 Fragments arranged by size: 6422 1960 220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2129 3346 3460 Size: 416 53 83 399 24 712 1217 114 Cuts at: 3460 3662 6333 7291 442 Size: 202 2671 958 1753 Fragments arranged by size: 2671 1753 1217 958 712 416 399 202 114 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2207 1056 Size: 1151 7451 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2469 5110 2469 Size: 2641 5961 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 2610 3227 3269 3642 3707 4007 7262 Size: 947 424 617 42 373 65 300 3255 Cuts at: 7262 1239 Size: 2579 Fragments arranged by size: 3255 2579 947 617 424 373 300 65 42 Bpu10I CC'TnA_GC Cuts at: 2894 3235 3269 3640 2894 Size: 341 34 371 7856 Fragments arranged by size: 7856 371 341 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2490 2596 3050 6727 7592 7890 2490 Size: 106 454 3677 865 298 3202 Fragments arranged by size: 3677 3202 865 454 298 106 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3923 7265 882 Size: 617 2424 3342 2219 Fragments arranged by size: 3342 2424 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3105 5899 6393 493 Size: 711 1901 2794 494 2702 Fragments arranged by size: 2794 2702 1901 711 494 BsaBI GATnn'nnATC Cuts at: 2835 8596 2835 Size: 5761 2841 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3865 5887 6409 6791 Size: 53 83 186 936 2332 2022 522 382 Cuts at: 6791 7426 275 Size: 635 1451 Fragments arranged by size: 2332 2022 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3338 4404 7035 7433 7707 7854 8265 3338 Size: 1066 2631 398 274 147 411 3675 Fragments arranged by size: 3675 2631 1066 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2306 2336 3200 3230 4108 Size: 30 165 30 903 30 864 30 878 Cuts at: 4108 4138 1178 Size: 30 5642 Fragments arranged by size: 5642 903 878 864 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3038 3140 3313 5648 1186 Size: 1852 102 173 2335 4140 Fragments arranged by size: 4140 2335 1852 173 102 BseYI C'CCAG_C Cuts at: 2329 2441 3631 3697 4464 5063 5792 7805 8301 Size: 112 1190 66 767 599 729 2013 496 Cuts at: 8301 2329 Size: 2630 Fragments arranged by size: 2630 2013 1190 767 729 599 496 112 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3228 3534 1163 Size: 573 1492 306 6231 Fragments arranged by size: 6231 1492 573 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5164 6052 6813 7841 1540 Size: 275 186 3163 888 761 1028 2301 Fragments arranged by size: 3163 2301 1028 888 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3497 3721 4400 6663 6748 7819 3497 Size: 224 679 2263 85 1071 4280 Fragments arranged by size: 4280 2263 1071 679 224 85 BsmI GAATG_Cn' Cuts at: 5882 6113 6206 5882 Size: 231 93 8278 Fragments arranged by size: 8278 231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3213 3497 3524 3697 3708 3721 4400 Size: 399 1423 284 27 173 11 13 679 Cuts at: 4400 5921 6663 6748 7819 1391 Size: 1521 742 85 1071 2174 Fragments arranged by size: 2174 1521 1423 1071 742 679 399 284 173 85 27 13 11 BspEI T'CCGG_A Cuts at: 8265 8265 Size: 8602 BspHI T'CATG_A Cuts at: 6491 8221 6491 Size: 1730 6872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3400 3418 3425 3469 5957 844 Size: 2556 18 7 44 2488 3489 Fragments arranged by size: 3489 2556 2488 44 18 7 BsrBI CCG'CTC Cuts at: 1394 1823 3691 6489 1394 Size: 429 1868 2798 3507 Fragments arranged by size: 3507 2798 1868 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4488 7097 7271 65 Size: 1183 3240 2609 174 1396 Fragments arranged by size: 3240 2609 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2964 3338 4404 7246 7433 1700 Size: 110 1154 374 1066 2842 187 2869 Fragments arranged by size: 2869 2842 1154 1066 374 187 110 BsrGI T'GTAC_A Cuts at: 96 2182 96 Size: 2086 6516 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8602 BssSI C'ACGA_G Cuts at: 6662 7674 6662 Size: 1012 7590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8602 BstBI TT'CG_AA Cuts at: 2007 7406 2007 Size: 5399 3203 BstEII G'GTnAC_C Cuts at: 1184 2609 1184 Size: 1425 7177 BstXI CCAn_nnnn'nTGG Cuts at: 2693 4691 2693 Size: 1998 6604 BstYI r'GATC_y Cuts at: 1972 2557 3150 3183 3331 3512 4003 4873 5932 Size: 585 593 33 148 181 491 870 1059 Cuts at: 5932 6299 6684 6701 8153 8239 8251 8597 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1059 870 593 585 491 385 367 346 181 148 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 5101 5101 Size: 8602 BtgI C'CryG_G Cuts at: 513 1065 3054 3676 3723 5539 513 Size: 552 1989 622 47 1816 3576 Fragments arranged by size: 3576 1989 1816 622 552 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7863 Fragments arranged by size: 7863 683 56 BtsI GCAGTG_nn' Cuts at: 2112 2292 3033 4669 5376 5839 6118 6912 6932 Size: 180 741 1636 707 463 279 794 20 Cuts at: 6932 2112 Size: 3782 Fragments arranged by size: 3782 1636 794 741 707 463 279 180 20 ClaI AT'CG_AT Cuts at: 1554 2154 1554 Size: 600 8002 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8567 DraI TTT'AAA Cuts at: 5906 5906 Size: 8602 DraIII CAC_nnn'GTG Cuts at: 3108 3557 3914 3108 Size: 449 357 7796 Fragments arranged by size: 7796 449 357 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2618 7609 809 Size: 507 1302 4991 1802 Fragments arranged by size: 4991 1802 1302 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2966 3073 6049 Size: 54 1425 50 275 18 1136 107 2976 Cuts at: 6049 6938 8567 8 Size: 889 1629 43 Fragments arranged by size: 2976 1629 1425 1136 889 275 107 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6049 1537 Size: 275 4237 4090 Fragments arranged by size: 4237 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2537 2639 3161 3986 4329 4713 4938 Size: 723 461 102 522 825 343 384 225 Cuts at: 4938 6532 1353 Size: 1594 3423 Fragments arranged by size: 3423 1594 825 723 522 461 384 343 225 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3865 4804 7189 7563 7709 387 Size: 1064 138 2276 939 2385 374 146 1280 Fragments arranged by size: 2385 2276 1280 1064 939 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2186 2610 3227 3269 3642 3707 Size: 393 468 86 424 617 42 373 65 Cuts at: 3707 4007 4737 4753 5253 5463 6665 7262 8049 Size: 300 730 16 500 210 1202 597 787 Cuts at: 8049 8317 1239 Size: 268 1524 Fragments arranged by size: 1524 1202 787 730 617 597 500 468 424 393 373 300 268 210 86 65 42 16 EcoO109I rG'GnC_Cy Cuts at: 3308 3694 4187 4711 3308 Size: 386 493 524 7199 Fragments arranged by size: 7199 524 493 386 EcoRI G'AATT_C Cuts at: 2631 5911 6289 2631 Size: 3280 378 4944 Fragments arranged by size: 4944 3280 378 EcoRV GAT'ATC Cuts at: 5419 5419 Size: 8602 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2690 3904 2690 Size: 1214 7388 FspI TGC'GCA Cuts at: 7108 7108 Size: 8602 HaeII r_GCGC'y Cuts at: 1678 7749 1678 Size: 6071 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 2306 2338 2339 Size: 32 135 32 689 32 13 32 1 Cuts at: 2339 2351 2371 2383 4281 4313 4721 4753 5097 Size: 12 20 12 1898 32 408 32 344 Cuts at: 5097 5129 7243 7275 7317 7349 1373 Size: 32 2114 32 42 32 2626 Fragments arranged by size: 2626 2114 1898 689 408 344 135 42 32 32 32 32 32 32 32 32 32 20 13 12 12 1 HincII GTy'rAC Cuts at: 669 1657 1938 2257 2536 3874 5407 5946 6192 Size: 988 281 319 279 1338 1533 539 246 Cuts at: 6192 669 Size: 3079 Fragments arranged by size: 3079 1533 1338 988 539 319 281 279 246 HindIII A'AGCT_T Cuts at: 748 3731 5660 748 Size: 2983 1929 3690 Fragments arranged by size: 3690 2983 1929 HpaI GTT'AAC Cuts at: 3874 6192 3874 Size: 2318 6284 KpnI G_GTAC'C Cuts at: 5927 5927 Size: 8602 MfeI C'AATT_G Cuts at: 6201 6201 Size: 8602 MluI A'CGCG_T Cuts at: 8478 8478 Size: 8602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2925 5328 7716 7900 1305 Size: 1620 2403 2388 184 2007 Fragments arranged by size: 2403 2388 2007 1620 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3075 10 Size: 54 1425 343 1243 5537 Fragments arranged by size: 5537 1425 1243 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 6560 6919 7078 518 Size: 648 5394 359 159 2042 Fragments arranged by size: 5394 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4476 4701 5402 6018 6695 7843 8088 1606 Size: 2870 225 701 616 677 1148 245 2120 Fragments arranged by size: 2870 2120 1148 701 677 616 245 225 NaeI GCC'GGC Cuts at: 2966 2966 Size: 8602 NcoI C'CATG_G Cuts at: 513 1065 3054 3676 3723 5539 513 Size: 552 1989 622 47 1816 3576 Fragments arranged by size: 3576 1989 1816 622 552 47 NdeI CA'TA_TG Cuts at: 387 4009 387 Size: 3622 4980 NgoMIV G'CCGG_C Cuts at: 2964 2964 Size: 8602 NheI G'CTAG_C Cuts at: 1052 2203 1052 Size: 1151 7451 NotI GC'GGCC_GC Cuts at: 6049 6049 Size: 8602 NsiI A_TGCA'T Cuts at: 2278 5271 2278 Size: 2993 5609 NspI r_CATG'y Cuts at: 1132 1229 2629 3685 4396 5960 7505 1132 Size: 97 1400 1056 711 1564 1545 2229 Fragments arranged by size: 2229 1564 1545 1400 1056 711 97 PasI CC'CwG_GG Cuts at: 1348 5757 1348 Size: 4409 4193 PciI A'CATG_T Cuts at: 4392 7501 4392 Size: 3109 5493 PflMI CCAn_nnn'nTGG Cuts at: 1108 4877 1108 Size: 3769 4833 PfoI T'CCnGG_A Cuts at: 3159 3984 3159 Size: 825 7777 PmeI GTTT'AAAC Cuts at: 5906 5906 Size: 8602 PmlI CAC'GTG Cuts at: 3105 3105 Size: 8602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3200 3232 4823 4855 6671 6703 8184 8216 3200 Size: 32 1591 32 1816 32 1481 32 3586 Fragments arranged by size: 3586 1816 1591 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4711 4711 Size: 8602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8602 PsiI TTA'TAA Cuts at: 6172 6172 Size: 8602 PspOMI G'GGCC_C Cuts at: 3693 3693 Size: 8602 PstI C_TGCA'G Cuts at: 830 3758 5954 830 Size: 2928 2196 3478 Fragments arranged by size: 3478 2928 2196 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8602 SalI G'TCGA_C Cuts at: 1936 5944 1936 Size: 4008 4594 SapI GCTCTTCn'nnn_ Cuts at: 2537 2537 Size: 8602 SbfI CC_TGCA'GG Cuts at: 5954 5954 Size: 8602 ScaI AGT'ACT Cuts at: 4580 6850 8562 4580 Size: 2270 1712 4620 Fragments arranged by size: 4620 2270 1712 SfcI C'TryA_G Cuts at: 826 1045 2067 2379 3381 3754 4141 4272 4436 Size: 219 1022 312 1002 373 387 131 164 Cuts at: 4436 4914 5397 5950 7085 7766 7957 826 Size: 478 483 553 1135 681 191 1471 Fragments arranged by size: 1471 1135 1022 1002 681 553 483 478 387 373 312 219 191 164 131 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8602 SmaI CCC'GGG Cuts at: 1872 4161 4817 5929 1872 Size: 2289 656 1112 4545 Fragments arranged by size: 4545 2289 1112 656 SmlI C'TyrA_G Cuts at: 820 1017 1943 2491 2505 2575 3065 3774 6706 Size: 197 926 548 14 70 490 709 2932 Cuts at: 6706 7607 7869 820 Size: 901 262 1553 Fragments arranged by size: 2932 1553 926 901 709 548 490 262 197 70 14 SnaBI TAC'GTA Cuts at: 493 5899 6393 493 Size: 5406 494 2702 Fragments arranged by size: 5406 2702 494 SpeI A'CTAG_T Cuts at: 152 5261 152 Size: 5109 3493 SphI G_CATG'C Cuts at: 1132 2629 3685 5960 1132 Size: 1497 1056 2275 3774 Fragments arranged by size: 3774 2275 1497 1056 SspI AAT'ATT Cuts at: 5 52 6526 5 Size: 47 6474 2081 Fragments arranged by size: 6474 2081 47 StuI AGG'CCT Cuts at: 1517 5093 1517 Size: 3576 5026 StyI C'CwwG_G Cuts at: 513 1065 3054 3676 3723 4854 5539 6041 513 Size: 552 1989 622 47 1131 685 502 3074 Fragments arranged by size: 3074 1989 1131 685 622 552 502 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2028 3278 4038 4212 4559 4966 5178 Size: 1255 448 1250 760 174 347 407 212 Cuts at: 5178 5749 6629 6646 6799 6984 7429 325 Size: 571 880 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1250 880 760 571 448 445 407 347 212 185 174 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2182 3321 4320 Size: 274 80 33 51 1443 205 1139 999 Cuts at: 4320 4578 6848 8560 96 Size: 258 2270 1712 138 Fragments arranged by size: 2270 1712 1443 1139 999 274 258 205 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3089 3121 4108 4140 8562 8594 3089 Size: 32 987 32 4422 32 3097 Fragments arranged by size: 4422 3097 987 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5326 1316 Size: 4010 4592 XbaI T'CTAG_A Cuts at: 5938 5938 Size: 8602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3052 4960 5624 3052 Size: 1908 664 6030 Fragments arranged by size: 6030 1908 664 XhoI C'TCGA_G Cuts at: 1943 3774 1943 Size: 1831 6771 XmaI C'CCGG_G Cuts at: 1870 4159 4815 5927 1870 Size: 2289 656 1112 4545 Fragments arranged by size: 4545 2289 1112 656 XmnI GAAnn'nnTTC Cuts at: 2315 3734 4086 5238 5726 6731 2315 Size: 1419 352 1152 488 1005 4186 Fragments arranged by size: 4186 1419 1152 1005 488 352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5888 6410 276 Size: 53 83 186 936 4354 522 2468 Fragments arranged by size: 4354 2468 936 522 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NarI NruI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI