Restriction map for
pFN21ASDA0065
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I FauI FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI MwoI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Alw44I 1045 GTGCAC G^TGCAC
AlwNI 79 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1045 GTGCAC G^TGCAC
AvaII 87 GGACC G^G[AT]CC
BbsI 2303 GAAGAC GAAGAC(2/6)
BclI 261 TGATCA T^GATCA
BfaI 92 CTAG C^TAG
BpmI 124 CTGGAG CTGGAG(16/14)
BpuAI 2303 GAAGAC GAAGAC(2/6)
BsrI 247 CCAGT ACTGG(1/-1)
Cfr13I 87 GGACC G^G[ACGT]CC
DraI 40 TTTAAA TTT^AAA
DraIII 2390 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 208 CCATGG C^C[GA][CT]GG
EcoNI 681 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 795 GATATC GAT^ATC
EcoT14I 208 CCATGG C^C[AT][AT]GG
EcoT22I 1496 ATGCAT ATGCA^T
FbaI 261 TGATCA T^GATCA
HincII 1692 GTCAAC GT[CT]^[GA]AC
HindII 1692 GTCAAC GT[CT]^[GA]AC
MaeI 92 CTAG C^TAG
MspA1I 257 CAGCTG C[AC]G^C[GT]G
NcoI 208 CCATGG C^CATGG
NsiI 1496 ATGCAT ATGCA^T
PshAI 88 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 257 CAGCTG CAG^CTG
SapI 202 GAAGAGC GCTCTTC(1/4)
Sau96I 87 GGACC G^G[ACGT]CC
SfcI 132 CTACAG C^T[GA][CT]AG
SspI 532 AATATT AAT^ATT
StyI 208 CCATGG C^C[AT][AT]GG
TaqI 1032 TCGA T^CGA
TthHB8I 1032 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AspHI 643 GAGCAC G[AT]GC[AT]^C
1045 GTGCAC
BanII 101 GGGCTC G[GA]GC[CT]^C
2135 GGGCTC
BsiHKAI 643 GAGCAC G[AT]GC[AT]^C
1045 GTGCAC
BsmAI 610 GAGAC GTCTC(1/5)
145 GTCTC
BsmI 1153 GAATGC GAATGC(1/-1)
2077 GAATGC
Bsp1407I 1956 TGTACA T^GTACA
2292 TGTACA
BspHI 603 TCATGA T^CATGA
1746 TCATGA
BsrDI 1157 GCAATG GCAATG(2/0)
2081 GCAATG
BsrGI 1956 TGTACA T^GTACA
2292 TGTACA
MslI 1384 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1747 CATGAATGTG
NlaIV 87 GGACCC GG[ACGT]^[ACGT]CC
278 GGAGCC
PleI 28 GAGTC GAGTC(4/5)
597 GAGTC
RcaI 603 TCATGA T^CATGA
1746 TCATGA
SmlI 557 CTCAAG C^T[CT][GA]AG
1411 CTTGAG
SspBI 1956 TGTACA T^GTACA
2292 TGTACA
TfiI 424 GATTC G^A[AT]TC
630 GATTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
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AcsI AfaI AluI ApoI Asp700I
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI DdeI DpnII EarI EcoRI
EcoRII Fnu4HI FokI HindIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NspI RsaI Sau3AI ScrFI
SfaNI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI