Restriction map for
pFN21ASDA0064
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbsI BclI BfrI
BglII BlnI BpuAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 873 CGCG CG^CG
AspHI 886 GAGCTC G[AT]GC[AT]^C
AspI 634 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1274 CCCGGG C^[CT]CG[GA]G
BalI 912 TGGCCA TGG^CCA
BanII 886 GAGCTC G[GA]GC[CT]^C
BbrPI 76 CACGTG CAC^GTG
BbvCI 208 GCTGAGG CCTCAGC(-5/-2)
BcgI 249 GCAAGCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 472 GGATAC GTATCC(6/5)
BfaI 1094 CTAG C^TAG
BpmI 427 CTGGAG CTGGAG(16/14)
BsiHKAI 886 GAGCTC G[AT]GC[AT]^C
BsmBI 1212 GAGACG CGTCTC(1/5)
BsmI 172 GCATTC GAATGC(1/-1)
BsoBI 1274 CCCGGG C^[CT]CG[GA]G
BstAPI 950 GCATGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 873 CGCG CG^CG
DraII 332 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 912 TGGCCA [CT]^GGCC[GA]
EarI 606 GAAGAG CTCTTC(1/4)
EcoO109I 332 AGGTCCC [GA]G^G[ACGT]CC[CT]
HincII 391 GTCAAC GT[CT]^[GA]AC
HindII 391 GTCAAC GT[CT]^[GA]AC
HindIII 187 AAGCTT A^AGCTT
Ksp632I 606 GAAGAG CTCTTC(1/4)
MaeI 1094 CTAG C^TAG
MluNI 912 TGGCCA TGG^CCA
MscI 912 TGGCCA TGG^CCA
MvnI 873 CGCG CG^CG
NaeI 1190 GCCGGC GCC^GGC
NcoI 1133 CCATGG C^CATGG
NgoMIV 1190 GCCGGC G^CCGGC
PflFI 634 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 76 CACGTG CAC^GTG
PmlI 76 CACGTG CAC^GTG
PpuMI 332 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 895 CTGCAG CTGCA^G
SacI 886 GAGCTC GAGCT^C
SgrAI 1189 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1274 CCCGGG CCC^GGG
SphI 950 GCATGC GCATG^C
Tth111I 634 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1274 CCCGGG C^CCGGG
XmaI 1274 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 115 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
790 CAGACGCTG
Asp700I 186 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
768 GAAGGAGTTC
AvaII 333 GGTCC G^G[AT]CC
1068 GGACC
BlpI 366 GCTCAGC GC^T[ACGT]AGC
888 GCTCAGC
BmyI 311 GGGCAC G[AGT]GC[ACT]^C
886 GAGCTC
Bpu1102I 366 GCTCAGC GC^T[ACGT]AGC
888 GCTCAGC
BsaAI 58 TACGTG [CT]AC^GT[GA]
76 CACGTG
BsaBI 1113 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1399 GATGAGGATC
BseRI 972 CTCCTC GAGGAG(10/8)
222 GAGGAG
BsgI 91 CTGCAC GTGCAG(16/14)
127 CTGCAC
Bsp1286I 311 GGGCAC G[AGT]GC[ACT]^C
886 GAGCTC
BspHI 1058 TCATGA T^CATGA
1109 TCATGA
BspMI 665 ACCTGC ACCTGC(4/8)
330 GCAGGT
BsrDI 269 CATTGC GCAATG(2/0)
983 CATTGC
BssSI 130 CACGAG CACGAG(-5/-1)
999 CACGAG
BstF5I 420 GGATG GGATG(2/0)
507 GGATG
CelII 366 GCTCAGC GC^T[ACGT]AGC
888 GCTCAGC
DsaI 1133 CCATGG C^C[GA][CT]GG
1368 CCACGG
FokI 420 GGATG GGATG(9/13)
507 GGATG
MamI 1113 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1399 GATGAGGATC
MspA1I 33 CAGCGG C[AC]G^C[GT]G
42 CAGCGG
NspI 245 ACATGC [GA]CATG^[CT]
950 GCATGC
PflMI 114 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
268 CCATTGCTTGG
RcaI 1058 TCATGA T^CATGA
1109 TCATGA
SmlI 1255 CTTGAG C^T[CT][GA]AG
1296 CTCAAG
TaqI 495 TCGA T^CGA
1385 TCGA
Tsp509I 1329 AATT ^AATT
1375 AATT
TthHB8I 495 TCGA T^CGA
1385 TCGA
Van91I 114 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
268 CCATTGCTTGG
XmnI 186 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
768 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BglI BsaJI BsiYI BslI
BsmAI BsmFI BsrFI BsrI BssKI
BstEII BstNI BstPI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EcoO65I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XhoII