(Circular) (Six-base) MAPSORT of: pFN21ASDA0064.seq Check: 788 from: 1 to: 6139 pFN21ASDA0064 6139 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3482 1937 Size: 1545 4594 Acc65I G'GTAC_C Cuts at: 3460 3460 Size: 6139 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2412 4202 5586 5854 1632 Size: 780 1790 1384 268 1917 Fragments arranged by size: 1917 1790 1384 780 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 5038 6015 1203 Size: 3835 977 1327 Fragments arranged by size: 3835 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3164 1184 Size: 1980 4159 AlwNI CAG_nnn'CTG Cuts at: 2143 2818 3882 5454 2143 Size: 675 1064 1572 2828 Fragments arranged by size: 2828 1572 1064 675 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 3297 3464 1870 Size: 73 1354 167 4545 Fragments arranged by size: 4545 1354 167 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2488 2521 2488 Size: 33 6106 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3460 4915 618 Size: 325 217 627 1673 1455 1842 Fragments arranged by size: 1842 1673 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2913 3458 1391 Size: 1522 545 4072 Fragments arranged by size: 4072 1522 545 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5168 BbvCI CC'TCA_GC Cuts at: 2232 2232 Size: 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2261 2295 4330 4364 1903 Size: 34 324 34 2035 34 3678 Fragments arranged by size: 3678 2035 324 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2489 4031 5241 2489 Size: 1542 1210 3387 Fragments arranged by size: 3387 1542 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 2855 2934 Size: 107 122 71 631 605 412 771 79 Cuts at: 2934 4750 136 Size: 1816 1525 Fragments arranged by size: 1816 1525 771 631 605 412 122 107 79 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 2390 2912 3556 2390 Size: 522 644 4973 Fragments arranged by size: 4973 644 522 Bme1580I G_kGCm'C Cuts at: 1790 2338 4200 5356 1790 Size: 548 1862 1156 2573 Fragments arranged by size: 2573 1862 1156 548 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3006 3870 4828 Size: 416 53 83 399 24 1589 864 958 Cuts at: 4828 442 Size: 1753 Fragments arranged by size: 1753 1589 958 864 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2800 2800 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2471 4799 1239 Size: 1232 2328 2579 Fragments arranged by size: 2579 2328 1232 Bpu10I CC'TnA_GC Cuts at: 2232 2624 2232 Size: 392 5747 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3299 3304 4264 5129 5427 3299 Size: 5 960 865 298 4011 Fragments arranged by size: 4011 960 865 298 5 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4802 882 Size: 617 3303 2219 Fragments arranged by size: 3303 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2083 2101 3436 3930 493 Size: 711 879 18 1335 494 2702 Fragments arranged by size: 2702 1335 879 711 494 18 BsaBI GATnn'nnATC Cuts at: 3140 3426 6133 3140 Size: 286 2707 3146 Fragments arranged by size: 3146 2707 286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3946 4328 4963 275 Size: 53 83 186 936 2413 382 635 1451 Fragments arranged by size: 2413 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5914 Fragments arranged by size: 5914 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2260 2986 1186 Size: 1074 726 4339 Fragments arranged by size: 4339 1074 726 BseYI C'CCAG_C Cuts at: 3362 5342 5838 3362 Size: 1980 496 3663 Fragments arranged by size: 3663 1980 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 2135 1163 Size: 573 363 36 5167 Fragments arranged by size: 5167 573 363 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3589 4350 5378 1540 Size: 275 186 1588 761 1028 2301 Fragments arranged by size: 2301 1588 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2913 4200 4285 5356 2913 Size: 1287 85 1071 3696 Fragments arranged by size: 3696 1287 1071 85 BsmI GAATG_Cn' Cuts at: 2195 3650 3743 2195 Size: 1455 93 4591 Fragments arranged by size: 4591 1455 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3229 3229 Size: 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2338 2913 3458 4200 4285 5356 1391 Size: 399 548 575 545 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 575 548 545 399 85 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 3081 3132 4028 5758 3081 Size: 51 896 1730 3462 Fragments arranged by size: 3462 1730 896 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2344 2697 3494 844 Size: 1500 353 797 3489 Fragments arranged by size: 3489 1500 797 353 BsrBI CCG'CTC Cuts at: 1394 1823 4026 1394 Size: 429 2203 3507 Fragments arranged by size: 3507 2203 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2291 3005 4634 4808 65 Size: 1183 1043 714 1629 174 1396 Fragments arranged by size: 1629 1396 1183 1043 714 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2754 3093 3213 4783 4970 1700 Size: 110 944 339 120 1570 187 2869 Fragments arranged by size: 2869 1570 944 339 187 120 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 2153 3022 4199 5211 2153 Size: 869 1177 1012 3081 Fragments arranged by size: 3081 1177 1012 869 BstAPI GCAn_nnn'nTGC Cuts at: 794 2979 794 Size: 2185 3954 BstBI TT'CG_AA Cuts at: 2007 4943 2007 Size: 2936 3203 BstEII G'GTnAC_C Cuts at: 1184 2737 2965 2989 3106 1184 Size: 1553 228 24 117 4217 Fragments arranged by size: 4217 1553 228 117 24 BstYI r'GATC_y Cuts at: 1972 2473 2730 3427 3469 3836 4221 4238 5690 Size: 501 257 697 42 367 385 17 1452 Cuts at: 5690 5776 5788 6134 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 697 501 385 367 346 257 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 3156 3391 513 Size: 552 2091 235 3261 Fragments arranged by size: 3261 2091 552 235 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2992 487 Size: 683 56 1766 3634 Fragments arranged by size: 3634 1766 683 56 BtsI GCAGTG_nn' Cuts at: 3264 3272 3655 4449 4469 3264 Size: 8 383 794 20 4934 Fragments arranged by size: 4934 794 383 20 8 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2935 3586 4475 Size: 54 1425 50 275 18 1105 651 889 Cuts at: 4475 6104 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1105 889 651 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3586 1537 Size: 275 1774 4090 Fragments arranged by size: 4090 1774 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2624 4069 1353 Size: 1271 1445 3423 Fragments arranged by size: 3423 1445 1271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2065 2915 4726 5100 5246 387 Size: 1064 138 476 850 1811 374 146 1280 Fragments arranged by size: 1811 1280 1064 850 476 374 146 138 EcoICRI GAG'CTC Cuts at: 2911 2911 Size: 6139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2412 2471 4202 4799 5586 5854 1239 Size: 393 780 59 1731 597 787 268 1524 Fragments arranged by size: 1731 1524 787 780 597 393 268 59 EcoO109I rG'GnC_Cy Cuts at: 2356 2356 Size: 6139 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 5286 1678 Size: 3608 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2357 2389 4780 4812 4854 Size: 32 135 32 785 32 2391 32 42 Cuts at: 4854 4886 1373 Size: 32 2626 Fragments arranged by size: 2626 2391 785 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2416 3483 3729 669 Size: 988 281 478 1067 246 3079 Fragments arranged by size: 3079 1067 988 478 281 246 HindIII A'AGCT_T Cuts at: 748 2210 748 Size: 1462 4677 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KpnI G_GTAC'C Cuts at: 3464 3464 Size: 6139 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5253 5437 1305 Size: 3948 184 2007 Fragments arranged by size: 3948 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2937 10 Size: 54 1425 343 1105 3212 Fragments arranged by size: 3212 1425 1105 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2103 2972 4097 4456 4615 518 Size: 648 933 4 869 1125 359 159 2042 Fragments arranged by size: 2042 1125 933 869 648 359 159 4 MspA1I CmG'CkG Cuts at: 1606 2058 2067 3555 4232 5380 5625 1606 Size: 452 9 1488 677 1148 245 2120 Fragments arranged by size: 2120 1488 1148 677 452 245 9 NaeI GCC'GGC Cuts at: 3215 3215 Size: 6139 NcoI C'CATG_G Cuts at: 513 1065 3156 513 Size: 552 2091 3496 Fragments arranged by size: 3496 2091 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NgoMIV G'CCGG_C Cuts at: 3213 3213 Size: 6139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 2272 2977 3497 5042 1132 Size: 97 1043 705 520 1545 2229 Fragments arranged by size: 2229 1545 1043 705 520 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 5038 5038 Size: 6139 PflMI CCAn_nnn'nTGG Cuts at: 1108 2143 2297 1108 Size: 1035 154 4950 Fragments arranged by size: 4950 1035 154 PfoI T'CCnGG_A Cuts at: 2448 2448 Size: 6139 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PmlI CAC'GTG Cuts at: 2101 2101 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2356 2356 Size: 6139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PstI C_TGCA'G Cuts at: 830 2922 3491 830 Size: 2092 569 3478 Fragments arranged by size: 3478 2092 569 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 SacI G_AGCT'C Cuts at: 2913 2913 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SfcI C'TryA_G Cuts at: 826 1045 2131 2457 2918 3098 3487 4622 5303 Size: 219 1086 326 461 180 389 1135 681 Cuts at: 5303 5494 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1086 681 461 389 326 219 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 3213 1700 Size: 1513 4626 SmaI CCC'GGG Cuts at: 1872 3299 3466 1872 Size: 1427 167 4545 Fragments arranged by size: 4545 1427 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 3278 3319 4243 5144 5406 820 Size: 197 926 1335 41 924 901 262 1553 Fragments arranged by size: 1553 1335 926 924 901 262 197 41 SnaBI TAC'GTA Cuts at: 493 3436 3930 493 Size: 2943 494 2702 Fragments arranged by size: 2943 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 2977 3497 1132 Size: 1845 520 3774 Fragments arranged by size: 3774 1845 520 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6139 StyI C'CwwG_G Cuts at: 513 1065 2376 2742 3156 3578 513 Size: 552 1311 366 414 422 3074 Fragments arranged by size: 3074 1311 552 422 414 366 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2715 4166 4183 4336 4521 4966 325 Size: 1255 1135 1451 17 153 185 445 1498 Fragments arranged by size: 1498 1451 1255 1135 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2265 4385 6097 Size: 274 80 33 51 1443 288 2120 1712 Cuts at: 6097 96 Size: 138 Fragments arranged by size: 2120 1712 1443 288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2340 2372 6099 6131 2340 Size: 32 3727 32 2348 Fragments arranged by size: 3727 2348 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2660 1316 Size: 1344 4795 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3297 3464 1870 Size: 1427 167 4545 Fragments arranged by size: 4545 1427 167 XmnI GAAnn'nnTTC Cuts at: 2213 2795 4268 2213 Size: 582 1473 4084 Fragments arranged by size: 4084 1473 582 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI