Restriction map for
pFN21ASDA0064
(Linear) (Six-base) MAPSORT of: KIAA0064.seq Check: 3018 from: 1 to: 1410
KIAA0064.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 1410
Size: 389 1021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1141 1410
Size: 1141 269
AlwNI CAG_nnn'CTG
Cuts at: 0 120 795 1410
Size: 120 675 615
Fragments arranged by size:
675 615 120
AvaI C'yCGr_G
Cuts at: 0 1274 1410
Size: 1274 136
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 465 498 1410
Size: 465 33 912
Fragments arranged by size:
912 465 33
BanII G_rGCy'C
Cuts at: 0 890 1410
Size: 890 520
BbvCI CC'TCA_GC
Cuts at: 0 209 1410
Size: 209 1201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 238 272 1410
Size: 238 34 1138
Fragments arranged by size:
1138 238 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 466 1410
Size: 466 944
BglI GCCn_nnn'nGGC
Cuts at: 0 61 832 911 1410
Size: 61 771 79 499
Fragments arranged by size:
771 499 79 61
BlpI GC'TnA_GC
Cuts at: 0 367 889 1410
Size: 367 522 521
Fragments arranged by size:
522 521 367
Bme1580I G_kGCm'C
Cuts at: 0 315 1410
Size: 315 1095
BmrI ACTGGGnnnn_n'
Cuts at: 0 983 1410
Size: 983 427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 777 1410
Size: 777 633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1410
Size: 448 962
Bpu10I CC'TnA_GC
Cuts at: 0 209 601 1410
Size: 209 392 809
Fragments arranged by size:
809 392 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1281 1410
Size: 1276 5 129
Fragments arranged by size:
1276 129 5
BsaAI yAC'GTr
Cuts at: 0 60 78 1410
Size: 60 18 1332
Fragments arranged by size:
1332 60 18
BsaBI GATnn'nnATC
Cuts at: 0 1117 1403 1410
Size: 1117 286 7
Fragments arranged by size:
1117 286 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 237 963 1410
Size: 237 726 447
Fragments arranged by size:
726 447 237
BseYI C'CCAG_C
Cuts at: 0 1339 1410
Size: 1339 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 112 1410
Size: 76 36 1298
Fragments arranged by size:
1298 76 36
BsiHKAI G_wGCw'C
Cuts at: 0 890 1410
Size: 890 520
BsmI GAATG_Cn'
Cuts at: 0 172 1410
Size: 172 1238
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1206 1410
Size: 1206 204
Bsp1286I G_dGCh'C
Cuts at: 0 315 890 1410
Size: 315 575 520
Fragments arranged by size:
575 520 315
BspHI T'CATG_A
Cuts at: 0 1058 1109 1410
Size: 1058 51 301
Fragments arranged by size:
1058 301 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 321 674 1410
Size: 321 353 736
Fragments arranged by size:
736 353 321
BsrDI GCAATG_nn'
Cuts at: 0 268 982 1410
Size: 268 714 428
Fragments arranged by size:
714 428 268
BsrFI r'CCGG_y
Cuts at: 0 731 1070 1190 1410
Size: 731 339 120 220
Fragments arranged by size:
731 339 220 120
BssSI C'ACGA_G
Cuts at: 0 130 999 1410
Size: 130 869 411
Fragments arranged by size:
869 411 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 956 1410
Size: 956 454
BstEII G'GTnAC_C
Cuts at: 0 714 942 966 1083 1410
Size: 714 228 24 117 327
Fragments arranged by size:
714 327 228 117 24
BstYI r'GATC_y
Cuts at: 0 450 707 1404 1410
Size: 450 257 697 6
Fragments arranged by size:
697 450 257 6
BtgI C'CryG_G
Cuts at: 0 1133 1368 1410
Size: 1133 235 42
Fragments arranged by size:
1133 235 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 969 1410
Size: 969 441
BtsI GCAGTG_nn'
Cuts at: 0 1241 1249 1410
Size: 1241 8 161
Fragments arranged by size:
1241 161 8
EaeI y'GGCC_r
Cuts at: 0 912 1410
Size: 912 498
EarI CTCTTCn'nnn_
Cuts at: 0 601 1410
Size: 601 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 42 892 1410
Size: 42 850 518
Fragments arranged by size:
850 518 42
EcoICRI GAG'CTC
Cuts at: 0 888 1410
Size: 888 522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 448 1410
Size: 389 59 962
Fragments arranged by size:
962 389 59
EcoO109I rG'GnC_Cy
Cuts at: 0 333 1410
Size: 333 1077
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 1410
Size: 334 32 1044
Fragments arranged by size:
1044 334 32
HincII GTy'rAC
Cuts at: 0 393 1410
Size: 393 1017
HindIII A'AGCT_T
Cuts at: 0 187 1410
Size: 187 1223
MscI TGG'CCA
Cuts at: 0 914 1410
Size: 914 496
MslI CAynn'nnrTG
Cuts at: 0 76 80 949 1410
Size: 76 4 869 461
Fragments arranged by size:
869 461 76 4
MspA1I CmG'CkG
Cuts at: 0 35 44 1410
Size: 35 9 1366
Fragments arranged by size:
1366 35 9
NaeI GCC'GGC
Cuts at: 0 1192 1410
Size: 1192 218
NcoI C'CATG_G
Cuts at: 0 1133 1410
Size: 1133 277
NgoMIV G'CCGG_C
Cuts at: 0 1190 1410
Size: 1190 220
NspI r_CATG'y
Cuts at: 0 249 954 1410
Size: 249 705 456
Fragments arranged by size:
705 456 249
PflMI CCAn_nnn'nTGG
Cuts at: 0 120 274 1410
Size: 120 154 1136
Fragments arranged by size:
1136 154 120
PfoI T'CCnGG_A
Cuts at: 0 425 1410
Size: 425 985
PmlI CAC'GTG
Cuts at: 0 78 1410
Size: 78 1332
PpuMI rG'GwC_Cy
Cuts at: 0 333 1410
Size: 333 1077
PstI C_TGCA'G
Cuts at: 0 899 1410
Size: 899 511
SacI G_AGCT'C
Cuts at: 0 890 1410
Size: 890 520
SfcI C'TryA_G
Cuts at: 0 108 434 895 1075 1410
Size: 108 326 461 180 335
Fragments arranged by size:
461 335 326 180 108
SgrAI Cr'CCGG_yG
Cuts at: 0 1190 1410
Size: 1190 220
SmaI CCC'GGG
Cuts at: 0 1276 1410
Size: 1276 134
SmlI C'TyrA_G
Cuts at: 0 1255 1296 1410
Size: 1255 41 114
Fragments arranged by size:
1255 114 41
SphI G_CATG'C
Cuts at: 0 954 1410
Size: 954 456
StyI C'CwwG_G
Cuts at: 0 353 719 1133 1410
Size: 353 366 414 277
Fragments arranged by size:
414 366 353 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 692 1410
Size: 692 718
TatI w'GTAC_w
Cuts at: 0 242 1410
Size: 242 1168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 349 1410
Size: 317 32 1061
Fragments arranged by size:
1061 317 32
Tth111I GACn'n_nGTC
Cuts at: 0 637 1410
Size: 637 773
XmaI C'CCGG_G
Cuts at: 0 1274 1410
Size: 1274 136
XmnI GAAnn'nnTTC
Cuts at: 0 190 772 1410
Size: 190 582 638
Fragments arranged by size:
638 582 190
Enzymes that do cut:
AcuI AlfI AlwNI AvaI BaeI BanII BbvCI BcgI
BciVI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI
BstEII BstYI BtgI BtgZI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I Hin4I HincII HindIII MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PmlI
PpuMI PstI SacI SfcI SgrAI SmaI SmlI SphI
StyI TaqII TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BamHI BanI BbeI BbsI BclI
BfrBI BglII BmgBI BmtI BsaI BsaHI BsaWI BsaXI
BsiEI BsiWI BspEI BsrBI BsrGI BssHII BstBI BstXI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XcmI XhoI ZraI