Restriction map for pFN21ASDA0063
 (Circular) (Six-base) MAPSORT of: pFN21ASDA0063.seq  Check: 2164  from: 1  to: 5335



pFN21ASDA0063  5335 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:29  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3145     278 
   Size:         53      83     186     936    1609    2468

  Fragments arranged by size: 

                2468    1609     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2678    1937 
   Size:        741    4594
 
Acc65I G'GTAC_C

Cuts at:   2584    2656    2584 
   Size:         72    5263
 
AclI AA'CG_TT

Cuts at:   1563    3462    3835    1563 
   Size:       1899     373    3063

  Fragments arranged by size: 

                3063    1899     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2279    3398    4782    5050    1632 
   Size:        647    1119    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1119     647     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5138
 
AflIII A'CryG_T

Cuts at:   1203    4234    5211    1203 
   Size:       3031     977    1327

  Fragments arranged by size: 

                3031    1327     977
 
AgeI A'CCGG_T

Cuts at:   4166    4166 
   Size:       5335
 
AhdI GACnn_n'nnGTC

Cuts at:   4064    4064 
   Size:       5335
 
AleI CACnn'nnGTG

Cuts at:   1166    2225    1166 
   Size:       1059    4276
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5335
 
AlwNI CAG_nnn'CTG

Cuts at:   3078    4650    3078 
   Size:       1572    3763
 
ApaLI G'TGCA_C

Cuts at:   3392    4548    3392 
   Size:       1156    4179
 
ApoI r'AATT_y

Cuts at:   1493    2644    2870    3022    5131    1493 
   Size:       1151     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1151     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3889     160 
   Size:        872    2857    1606

  Fragments arranged by size: 

                2857    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5335
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2178    2501    2660    1870 
   Size:         73     235     323     159    4545

  Fragments arranged by size: 

                4545     323     235     159      73
 
BamHI G'GATC_C

Cuts at:   2665    3032    2665 
   Size:        367    4968
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2584    2656    4111     618 
   Size:        325     217     627     797      72    1455    1842

  Fragments arranged by size: 

                1842    1455     797     627     325     217      72
 
BanII G_rGCy'C

Cuts at:   1391    2529    2654    1391 
   Size:       1138     125    4072

  Fragments arranged by size: 

                4072    1138     125
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2146     928 
   Size:        971     247    4117

  Fragments arranged by size: 

                4117     971     247
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2323    2357    3526    3560    1903 
   Size:         34     386      34    1169      34    3678

  Fragments arranged by size: 

                3678    1169     386      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2176    3227    4437    2176 
   Size:       1051    1210    3074

  Fragments arranged by size: 

                3074    1210    1051
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5335
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3946     136 
   Size:        107     122      71     631     605    2274    1525

  Fragments arranged by size: 

                2274    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5330    5330 
   Size:       5335
 
BlpI GC'TnA_GC

Cuts at:   2752    2752 
   Size:       5335
 
Bme1580I G_kGCm'C

Cuts at:   1790    2134    3396    4552    1790 
   Size:        344    1262    1156    2573

  Fragments arranged by size: 

                2573    1262    1156     344
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5335
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3066    4024     442 
   Size:        416      53      83     399      24    1649     958    1753

  Fragments arranged by size: 

                1753    1649     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5335
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2229    2229 
   Size:       5335
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2377    3995    1239 
   Size:       1138    1618    2579

  Fragments arranged by size: 

                2579    1618    1138
 
Bpu10I CC'TnA_GC

Cuts at:   2528    2528 
   Size:       5335
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2477    3460    4325    4623    2477 
   Size:        983     865     298    3189

  Fragments arranged by size: 

                3189     983     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3998     882 
   Size:        617    2499    2219

  Fragments arranged by size: 

                2499    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2632    3126     493 
   Size:        711    1428     494    2702

  Fragments arranged by size: 

                2702    1428     711     494
 
BsaBI GATnn'nnATC

Cuts at:   5329    5329 
   Size:       5335
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3142    3524    4159     275 
   Size:         53      83     186     936    1609     382     635    1451

  Fragments arranged by size: 

                1609    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3768    4166    4440    4587    4998    3768 
   Size:        398     274     147     411    4105

  Fragments arranged by size: 

                4105     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2468    2498    1178 
   Size:         30     165      30    1065      30    4015

  Fragments arranged by size: 

                4015    1065     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5335
 
BseYI C'CCAG_C

Cuts at:   2251    2403    4538    5034    2251 
   Size:        152    2135     496    2552

  Fragments arranged by size: 

                2552    2135     496     152
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4762
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2785    3546    4574    1540 
   Size:        275     186     784     761    1028    2301

  Fragments arranged by size: 

                2301    1028     784     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2529    3396    3481    4552    2529 
   Size:        867      85    1071    3312

  Fragments arranged by size: 

                3312    1071     867      85
 
BsmI GAATG_Cn'

Cuts at:   2846    2939    2846 
   Size:         93    5242
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2134    2529    2654    3396    3481    4552    1391 
   Size:        399     344     395     125     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     395     344     125      85
            
 
BspEI T'CCGG_A

Cuts at:   4998    4998 
   Size:       5335
 
BspHI T'CATG_A

Cuts at:   2379    3224    4954    2379 
   Size:        845    1730    2760

  Fragments arranged by size: 

                2760    1730     845
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2690     844 
   Size:       1846    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3222    1394 
   Size:        429    1399    3507

  Fragments arranged by size: 

                3507    1399     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2173    2349    3830    4004      65 
   Size:       1183     925     176    1481     174    1396

  Fragments arranged by size: 

                1481    1396    1183     925     176     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3979    4166    1700 
   Size:        110    2169     187    2869

  Fragments arranged by size: 

                2869    2169     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5335
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5335
 
BssSI C'ACGA_G

Cuts at:   3395    4407    3395 
   Size:       1012    4323
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5335
 
BstBI TT'CG_AA

Cuts at:   2007    4139    2007 
   Size:       2132    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5335
 
BstYI r'GATC_y

Cuts at:   1972    2442    2665    3032    3417    3434    4886    4972    4984 
   Size:        470     223     367     385      17    1452      86      12

Cuts at:   4984    5330    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     470     385     367     346     223      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2036    2223     513 
   Size:        552     971     187    3625

  Fragments arranged by size: 

                3625     971     552     187
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4596

  Fragments arranged by size: 

                4596     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2851    3645    3665    2851 
   Size:        794      20    4521

  Fragments arranged by size: 

                4521     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5335
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5300
 
DraI TTT'AAA

Cuts at:   2639    2639 
   Size:       5335
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4342     809 
   Size:        507    3026    1802

  Fragments arranged by size: 

                3026    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2782    3671    5300 
   Size:         54    1425      50     275      18     952     889    1629

Cuts at:   5300       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     952     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2782    1537 
   Size:        275     970    4090

  Fragments arranged by size: 

                4090     970     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2237    2586    3265    1353 
   Size:        884     349     679    3423

  Fragments arranged by size: 

                3423     884     679     349
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3922    4296    4442     387 
   Size:       1064     138    2333     374     146    1280

  Fragments arranged by size: 

                2333    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2527    2527 
   Size:       5335
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2279    2377    3398    3995    4782    5050    1239 
   Size:        393     647      98    1021     597     787     268    1524

  Fragments arranged by size: 

                1524    1021     787     647     597     393     268      98
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2466    2466 
   Size:       5335
 
EcoRI G'AATT_C

Cuts at:   2644    3022    2644 
   Size:        378    4957
 
FspI TGC'GCA

Cuts at:   3841    3841 
   Size:       5335
 
HaeII r_GCGC'y

Cuts at:   1678    4482    1678 
   Size:       2804    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3976    4008    4050    4082    1373 
   Size:         32     135      32    2404      32      42      32    2626

  Fragments arranged by size: 

                2626    2404     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2679    2925     669 
   Size:        988     281     741     246    3079

  Fragments arranged by size: 

                3079     988     741     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5335
 
HpaI GTT'AAC

Cuts at:   2925    2925 
   Size:       5335
 
KpnI G_GTAC'C

Cuts at:   2588    2660    2588 
   Size:         72    5263
 
MfeI C'AATT_G

Cuts at:   2934    2934 
   Size:       5335
 
MluI A'CGCG_T

Cuts at:   5211    5211 
   Size:       5335
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2594    4449    4633    1305 
   Size:       1289    1855     184    2007

  Fragments arranged by size: 

                2007    1855    1289     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3513

  Fragments arranged by size: 

                3513    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2225    3293    3652    3811     518 
   Size:        648    1059    1068     359     159    2042

  Fragments arranged by size: 

                2042    1068    1059     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2751    3428    4576    4821    1606 
   Size:       1145     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1145     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2036    2223     513 
   Size:        552     971     187    3625

  Fragments arranged by size: 

                3625     971     552     187
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5335
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5335
 
NotI GC'GGCC_GC

Cuts at:   2782    2782 
   Size:       5335
 
NspI r_CATG'y

Cuts at:   1132    1229    2251    2693    4238    1132 
   Size:         97    1022     442    1545    2229

  Fragments arranged by size: 

                2229    1545    1022     442      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5335
 
PciI A'CATG_T

Cuts at:   4234    4234 
   Size:       5335
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5335
 
PfoI T'CCnGG_A

Cuts at:   2157    2157 
   Size:       5335
 
PmeI GTTT'AAAC

Cuts at:   2639    2639 
   Size:       5335
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3404    3436    4917    4949    3404 
   Size:         32    1481      32    3790

  Fragments arranged by size: 

                3790    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5335
 
PsiI TTA'TAA

Cuts at:   2905    2905 
   Size:       5335
 
PstI C_TGCA'G

Cuts at:    830    2687     830 
   Size:       1857    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5335
 
SacI G_AGCT'C

Cuts at:   2529    2529 
   Size:       5335
 
SalI G'TCGA_C

Cuts at:   1936    2677    1936 
   Size:        741    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2237    2237 
   Size:       5335
 
SbfI CC_TGCA'GG

Cuts at:   2687    2687 
   Size:       5335
 
ScaI AGT'ACT

Cuts at:   3583    5295    3583 
   Size:       1712    3623
 
SfcI C'TryA_G

Cuts at:    826    1045    2683    3818    4499    4690     826 
   Size:        219    1638    1135     681     191    1471

  Fragments arranged by size: 

                1638    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5335
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5335
 
SmaI CCC'GGG

Cuts at:   1872    2180    2662    1872 
   Size:        308     482    4545

  Fragments arranged by size: 

                4545     482     308
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2492    3439    4340    4602     820 
   Size:        197     926     549     947     901     262    1553

  Fragments arranged by size: 

                1553     947     926     901     549     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2632    3126     493 
   Size:       2139     494    2702

  Fragments arranged by size: 

                2702    2139     494
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5335
 
SphI G_CATG'C

Cuts at:   1132    2251    2693    1132 
   Size:       1119     442    3774

  Fragments arranged by size: 

                3774    1119     442
 
SspI AAT'ATT

Cuts at:      5      52    3259       5 
   Size:         47    3207    2081

  Fragments arranged by size: 

                3207    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5335
 
StyI C'CwwG_G

Cuts at:    513    1065    2036    2223    2774     513 
   Size:        552     971     187     551    3074

  Fragments arranged by size: 

                3074     971     552     551     187
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2636    3362    3379    3532    3717    4162     325 
   Size:       1255    1056     726      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1056     726     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3581    5293      96 
   Size:        274      80      33      51    1443    1604    1712     138

  Fragments arranged by size: 

                1712    1604    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5295    5327    5295 
   Size:         32    5303
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5335
 
XbaI T'CTAG_A

Cuts at:   2671    2671 
   Size:       5335
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5335
 
XmaI C'CCGG_G

Cuts at:   1870    2178    2660    1870 
   Size:        308     482    4545

  Fragments arranged by size: 

                4545     482     308
 
XmnI GAAnn'nnTTC

Cuts at:   3464    3464 
   Size:       5335
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3143     276 
   Size:         53      83     186     936    1609    2468

  Fragments arranged by size: 

                2468    1609     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII    SacII    SanDI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com