(Circular) (Six-base) MAPSORT of: pFN21ASDA0062.seq Check: 6631 from: 1 to: 6205 pFN21ASDA0062 6205 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 4268 5652 5920 1632 Size: 596 2040 1384 268 1917 Fragments arranged by size: 2040 1917 1384 596 268 AfeI AGC'GCT Cuts at: 2140 2470 2140 Size: 330 5875 AflII C'TTAA_G Cuts at: 820 1017 2756 820 Size: 197 1739 4269 Fragments arranged by size: 4269 1739 197 AflIII A'CryG_T Cuts at: 1203 5104 6081 1203 Size: 3901 977 1327 Fragments arranged by size: 3901 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 3131 1166 Size: 1965 4240 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 2206 2395 2965 3430 3948 5520 2206 Size: 189 570 465 518 1572 2891 Fragments arranged by size: 2891 1572 570 518 465 189 ApaLI G'TGCA_C Cuts at: 2124 4262 5418 2124 Size: 2138 1156 2911 Fragments arranged by size: 2911 2138 1156 ApoI r'AATT_y Cuts at: 1493 3514 3740 3892 6001 1493 Size: 2021 226 152 2109 1697 Fragments arranged by size: 2109 2021 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2403 3530 1870 Size: 73 460 1127 4545 Fragments arranged by size: 4545 1127 460 73 BamHI G'GATC_C Cuts at: 3535 3902 3535 Size: 367 5838 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2545 2914 3526 4981 618 Size: 325 217 627 758 369 612 1455 1842 Fragments arranged by size: 1842 1455 758 627 612 369 325 217 BanII G_rGCy'C Cuts at: 1391 2364 2948 3524 1391 Size: 973 584 576 4072 Fragments arranged by size: 4072 973 584 576 BbeI G_GCGC'C Cuts at: 2918 2918 Size: 6205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2567 928 Size: 971 668 4566 Fragments arranged by size: 4566 971 668 BbvCI CC'TCA_GC Cuts at: 2883 2883 Size: 6205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4396 4430 1903 Size: 34 2459 34 3678 Fragments arranged by size: 3678 2459 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2480 4097 5307 2480 Size: 1617 1210 3378 Fragments arranged by size: 3378 1617 1210 BclI T'GATC_A Cuts at: 1548 3039 1548 Size: 1491 4714 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4816 136 Size: 107 122 71 631 605 3144 1525 Fragments arranged by size: 3144 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2739 6200 2739 Size: 3461 2744 BlpI GC'TnA_GC Cuts at: 3622 3622 Size: 6205 Bme1580I G_kGCm'C Cuts at: 1790 2128 2550 4266 5422 1790 Size: 338 422 1716 1156 2573 Fragments arranged by size: 2573 1716 1156 422 338 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3936 4894 442 Size: 416 53 83 399 24 2519 958 1753 Fragments arranged by size: 2519 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3317 1056 Size: 2261 3944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2325 2348 2372 3339 4865 1239 Size: 1086 23 24 967 1526 2579 Fragments arranged by size: 2579 1526 1086 967 24 23 Bpu10I CC'TnA_GC Cuts at: 2883 2883 Size: 6205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4330 5195 5493 4330 Size: 865 298 5042 Fragments arranged by size: 5042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2301 2498 4868 882 Size: 617 802 197 2370 2219 Fragments arranged by size: 2370 2219 802 617 197 BsaAI yAC'GTr Cuts at: 493 1204 2266 2292 3502 3996 493 Size: 711 1062 26 1210 494 2702 Fragments arranged by size: 2702 1210 1062 711 494 26 BsaBI GATnn'nnATC Cuts at: 3203 6199 3203 Size: 2996 3209 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2915 4012 4394 5029 Size: 53 83 186 936 1382 1097 382 635 Cuts at: 5029 275 Size: 1451 Fragments arranged by size: 1451 1382 1097 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4638 5036 5310 5457 5868 4638 Size: 398 274 147 411 4975 Fragments arranged by size: 4975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3162 3192 1178 Size: 30 165 30 1759 30 4191 Fragments arranged by size: 4191 1759 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2450 2468 2486 2846 3164 1186 Size: 1264 18 18 360 318 4227 Fragments arranged by size: 4227 1264 360 318 18 18 BseYI C'CCAG_C Cuts at: 2259 3194 5408 5904 2259 Size: 935 2214 496 2560 Fragments arranged by size: 2560 2214 935 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2024 2395 2978 1163 Size: 573 288 371 583 4390 Fragments arranged by size: 4390 583 573 371 288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2128 2364 4266 4351 5422 2128 Size: 236 1902 85 1071 2911 Fragments arranged by size: 2911 1902 1071 236 85 BsmI GAATG_Cn' Cuts at: 3716 3809 3716 Size: 93 6112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2364 2550 2948 3524 4266 4351 Size: 399 338 236 186 398 576 742 85 Cuts at: 4351 5422 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 576 399 398 338 236 186 85 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 2553 4094 5824 2553 Size: 1541 1730 2934 Fragments arranged by size: 2934 1730 1541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2973 3560 844 Size: 2129 587 3489 Fragments arranged by size: 3489 2129 587 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4700 4874 65 Size: 1183 3452 174 1396 Fragments arranged by size: 3452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3278 4849 5036 1700 Size: 110 1468 1571 187 2869 Fragments arranged by size: 2869 1571 1468 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 2965 794 Size: 2171 4034 BstBI TT'CG_AA Cuts at: 2007 5009 2007 Size: 3002 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 3460 3460 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2158 2739 3486 3535 3902 4287 4304 5756 Size: 186 581 747 49 367 385 17 1452 Cuts at: 5756 5842 5854 6200 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 747 581 385 367 346 186 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2100 2100 Size: 6205 BtgI C'CryG_G Cuts at: 513 1065 2919 513 Size: 552 1854 3799 Fragments arranged by size: 3799 1854 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 2316 2703 2887 2902 3721 4515 4535 2316 Size: 387 184 15 819 794 20 3986 Fragments arranged by size: 3986 819 794 387 184 20 15 ClaI AT'CG_AT Cuts at: 1554 3072 1554 Size: 1518 4687 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2933 5212 809 Size: 507 1617 2279 1802 Fragments arranged by size: 2279 1802 1617 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2462 3081 3135 Size: 54 1425 50 275 18 632 619 54 Cuts at: 3135 3276 3652 4541 6170 8 Size: 141 376 889 1629 43 Fragments arranged by size: 1629 1425 889 632 619 376 275 141 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 2636 3224 4135 1353 Size: 965 318 588 911 3423 Fragments arranged by size: 3423 965 911 588 318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 2362 Size: 6205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2325 2348 2372 3339 4268 4865 Size: 393 596 97 23 24 967 929 597 Cuts at: 4865 5652 5920 1239 Size: 787 268 1524 Fragments arranged by size: 1524 967 929 787 597 596 393 268 97 24 23 EcoNI CCTnn'n_nnAGG Cuts at: 3146 3146 Size: 6205 EcoO109I rG'GnC_Cy Cuts at: 2212 2863 2962 2212 Size: 651 99 5455 Fragments arranged by size: 5455 651 99 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 EcoRV GAT'ATC Cuts at: 3019 3019 Size: 6205 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 6205 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 2142 2472 2918 5352 1678 Size: 464 330 446 2434 2531 Fragments arranged by size: 2531 2434 464 446 330 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2478 2510 3028 3060 3162 Size: 32 135 32 906 32 518 32 102 Cuts at: 3162 3194 3438 3470 4846 4878 4920 4952 1373 Size: 32 244 32 1376 32 42 32 2626 Fragments arranged by size: 2626 1376 906 518 244 135 102 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3549 3795 669 Size: 988 281 1611 246 3079 Fragments arranged by size: 3079 1611 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KasI G'GCGC_C Cuts at: 2914 2914 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2646 3232 5319 5503 1305 Size: 1341 586 2087 184 2007 Fragments arranged by size: 2087 2007 1341 586 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3083 3137 10 Size: 54 1425 343 1251 54 3078 Fragments arranged by size: 3078 1425 1251 343 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 2229 2778 3131 3455 3458 4163 Size: 648 1006 57 549 353 324 3 705 Cuts at: 4163 4522 4681 518 Size: 359 159 2042 Fragments arranged by size: 2042 1006 705 648 549 359 353 324 159 57 3 MspA1I CmG'CkG Cuts at: 1606 2206 2395 3621 4298 5446 5691 1606 Size: 600 189 1226 677 1148 245 2120 Fragments arranged by size: 2120 1226 1148 677 600 245 189 NaeI GCC'GGC Cuts at: 3280 3280 Size: 6205 NarI GG'CG_CC Cuts at: 2915 2915 Size: 6205 NcoI C'CATG_G Cuts at: 513 1065 2919 513 Size: 552 1854 3799 Fragments arranged by size: 3799 1854 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NgoMIV G'CCGG_C Cuts at: 3278 3278 Size: 6205 NheI G'CTAG_C Cuts at: 1052 3313 1052 Size: 2261 3944 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 3563 5108 1132 Size: 97 2334 1545 2229 Fragments arranged by size: 2334 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2119 1348 Size: 771 5434 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2395 3430 1108 Size: 1287 1035 3883 Fragments arranged by size: 3883 1287 1035 PfoI T'CCnGG_A Cuts at: 2364 2643 2364 Size: 279 5926 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PmlI CAC'GTG Cuts at: 2266 2292 2266 Size: 26 6179 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2863 2962 2863 Size: 99 6106 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PstI C_TGCA'G Cuts at: 830 2157 2202 2698 2856 2894 2970 3557 830 Size: 1327 45 496 158 38 76 587 3478 Fragments arranged by size: 3478 1327 587 496 158 76 45 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 PvuII CAG'CTG Cuts at: 2206 2395 2206 Size: 189 6016 SacI G_AGCT'C Cuts at: 2364 2364 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 2157 2970 3557 2157 Size: 813 587 4805 Fragments arranged by size: 4805 813 587 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 2153 2198 2694 2852 2890 2966 3553 Size: 219 1108 45 496 158 38 76 587 Cuts at: 3553 4688 5369 5560 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 681 587 496 219 191 158 76 45 38 SfoI GGC'GCC Cuts at: 2916 2916 Size: 6205 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 2405 3532 1872 Size: 533 1127 4545 Fragments arranged by size: 4545 1127 533 SmlI C'TyrA_G Cuts at: 820 1017 1943 2756 4309 5210 5472 820 Size: 197 926 813 1553 901 262 1553 Fragments arranged by size: 1553 1553 926 901 813 262 197 SnaBI TAC'GTA Cuts at: 493 3502 3996 493 Size: 3009 494 2702 Fragments arranged by size: 3009 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 2919 3115 3644 513 Size: 552 1854 196 529 3074 Fragments arranged by size: 3074 1854 552 529 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 2273 4232 4249 4402 4587 5032 Size: 1255 531 162 1959 17 153 185 445 Cuts at: 5032 325 Size: 1498 Fragments arranged by size: 1959 1498 1255 531 445 185 162 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4451 6163 96 Size: 274 80 33 51 1443 2474 1712 138 Fragments arranged by size: 2474 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2099 2131 6165 6197 2099 Size: 32 4034 32 2107 Fragments arranged by size: 4034 2107 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3176 1316 Size: 1860 4345 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2695 2695 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 2403 3530 1870 Size: 533 1127 4545 Fragments arranged by size: 4545 1127 533 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI