(Circular) (Six-base) MAPSORT of: pFN21ASDA0057.seq Check: 7225 from: 1 to: 5839 pFN21ASDA0057 5839 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 1937 Size: 1245 4594 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2044 3902 5286 5554 1632 Size: 412 1858 1384 268 1917 Fragments arranged by size: 1917 1858 1384 412 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 4738 5715 1203 Size: 3535 977 1327 Fragments arranged by size: 3535 1327 977 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 2381 4568 2381 Size: 2187 3652 AleI CACnn'nnGTG Cuts at: 1166 2402 1166 Size: 1236 4603 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3086 3118 3086 Size: 32 5807 AlwNI CAG_nnn'CTG Cuts at: 2763 2901 3582 5154 2763 Size: 138 681 1572 3448 Fragments arranged by size: 3448 1572 681 138 ApaLI G'TGCA_C Cuts at: 2219 2483 3896 5052 2219 Size: 264 1413 1156 3006 Fragments arranged by size: 3006 1413 1156 264 ApoI r'AATT_y Cuts at: 1493 2583 3148 3374 3526 5635 1493 Size: 1090 565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1090 565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 2864 3164 1870 Size: 73 921 300 4545 Fragments arranged by size: 4545 921 300 73 BamHI G'GATC_C Cuts at: 3169 3536 3169 Size: 367 5472 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 4615 618 Size: 325 217 627 1373 1455 1842 Fragments arranged by size: 1842 1455 1373 627 325 217 BanII G_rGCy'C Cuts at: 1391 3158 1391 Size: 1767 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2475 928 Size: 971 576 4292 Fragments arranged by size: 4292 971 576 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4030 4064 1903 Size: 34 2093 34 3678 Fragments arranged by size: 3678 2093 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2432 3731 4941 2432 Size: 1299 1210 3330 Fragments arranged by size: 3330 1299 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2661 2816 2930 Size: 107 122 71 631 605 989 155 114 Cuts at: 2930 4450 136 Size: 1520 1525 Fragments arranged by size: 1525 1520 989 631 605 155 122 114 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 3256 3256 Size: 5839 Bme1580I G_kGCm'C Cuts at: 1790 2223 2487 3900 5056 1790 Size: 433 264 1413 1156 2573 Fragments arranged by size: 2573 1413 1156 433 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3570 4528 442 Size: 416 53 83 399 24 2153 958 1753 Fragments arranged by size: 2153 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2657 2657 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2582 2652 4499 1239 Size: 1343 70 1847 2579 Fragments arranged by size: 2579 1847 1343 70 Bpu10I CC'TnA_GC Cuts at: 2550 2651 2550 Size: 101 5738 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3107 3964 4829 5127 3107 Size: 857 865 298 3819 Fragments arranged by size: 3819 865 857 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2207 4502 882 Size: 617 708 2295 2219 Fragments arranged by size: 2295 2219 708 617 BsaAI yAC'GTr Cuts at: 493 1204 2422 3136 3630 493 Size: 711 1218 714 494 2702 Fragments arranged by size: 2702 1218 714 711 494 BsaBI GATnn'nnATC Cuts at: 5833 5833 Size: 5839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3646 4028 4663 275 Size: 53 83 186 936 2113 382 635 1451 Fragments arranged by size: 2113 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4272 4670 4944 5091 5502 4272 Size: 398 274 147 411 4609 Fragments arranged by size: 4609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3086 3116 1178 Size: 30 165 30 1683 30 3901 Fragments arranged by size: 3901 1683 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5839 BseYI C'CCAG_C Cuts at: 2961 3016 5042 5538 2961 Size: 55 2026 496 3262 Fragments arranged by size: 3262 2026 496 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2522 1163 Size: 573 786 4480 Fragments arranged by size: 4480 786 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2223 2487 3900 3985 5056 2115 Size: 108 264 1413 85 1071 2898 Fragments arranged by size: 2898 1413 1071 264 108 85 BsmI GAATG_Cn' Cuts at: 3350 3443 3350 Size: 93 5746 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2223 2487 3158 3900 3985 5056 Size: 399 325 108 264 671 742 85 1071 Cuts at: 5056 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 671 399 325 264 108 85 BspEI T'CCGG_A Cuts at: 5502 5502 Size: 5839 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3194 844 Size: 2350 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2056 3726 1394 Size: 429 233 1670 3507 Fragments arranged by size: 3507 1670 429 233 BsrDI GCAATG_nn' Cuts at: 65 1248 4334 4508 65 Size: 1183 3086 174 1396 Fragments arranged by size: 3086 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4483 4670 1700 Size: 110 2673 187 2869 Fragments arranged by size: 2869 2673 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 3899 4911 3899 Size: 1012 4827 BstAPI GCAn_nnn'nTGC Cuts at: 794 2901 794 Size: 2107 3732 BstBI TT'CG_AA Cuts at: 2007 4643 2007 Size: 2636 3203 BstEII G'GTnAC_C Cuts at: 1184 2782 3062 1184 Size: 1598 280 3961 Fragments arranged by size: 3961 1598 280 BstXI CCAn_nnnn'nTGG Cuts at: 3074 3074 Size: 5839 BstYI r'GATC_y Cuts at: 1972 3169 3536 3921 3938 5390 5476 5488 5834 Size: 1197 367 385 17 1452 86 12 346 Cuts at: 5834 1972 Size: 1977 Fragments arranged by size: 1977 1452 1197 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2238 2238 Size: 5839 BtgI C'CryG_G Cuts at: 513 1065 2022 3107 513 Size: 552 957 1085 3245 Fragments arranged by size: 3245 1085 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5100 Fragments arranged by size: 5100 683 56 BtsI GCAGTG_nn' Cuts at: 3355 4149 4169 3355 Size: 794 20 5025 Fragments arranged by size: 5025 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2817 3286 4175 Size: 54 1425 50 275 18 987 469 889 Cuts at: 4175 5804 8 Size: 1629 43 Fragments arranged by size: 1629 1425 987 889 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2708 2726 2798 3769 1353 Size: 710 645 18 72 971 3423 Fragments arranged by size: 3423 971 710 645 72 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4426 4800 4946 387 Size: 1064 138 2837 374 146 1280 Fragments arranged by size: 2837 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2582 2652 3902 4499 5286 5554 Size: 393 412 538 70 1250 597 787 268 Cuts at: 5554 1239 Size: 1524 Fragments arranged by size: 1524 1250 787 597 538 412 393 268 70 EcoO109I rG'GnC_Cy Cuts at: 2242 2242 Size: 5839 EcoRI G'AATT_C Cuts at: 3148 3526 3148 Size: 378 5461 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 5839 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 2950 4986 1678 Size: 1272 2036 2531 Fragments arranged by size: 2531 2036 1272 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2367 2399 4480 4512 4554 Size: 32 135 32 795 32 2081 32 42 Cuts at: 4554 4586 1373 Size: 32 2626 Fragments arranged by size: 2626 2081 795 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3429 669 Size: 988 281 1245 246 3079 Fragments arranged by size: 3079 1245 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4953 5137 1305 Size: 3648 184 2007 Fragments arranged by size: 3648 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2819 10 Size: 54 1425 343 987 3030 Fragments arranged by size: 3030 1425 987 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 2192 2402 3072 3797 4156 4315 Size: 648 919 107 210 670 725 359 159 Cuts at: 4315 518 Size: 2042 Fragments arranged by size: 2042 919 725 670 648 359 210 159 107 MspA1I CmG'CkG Cuts at: 1606 2380 2524 2965 3255 3932 5080 5325 1606 Size: 774 144 441 290 677 1148 245 2120 Fragments arranged by size: 2120 1148 774 677 441 290 245 144 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4330 Fragments arranged by size: 4330 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 2291 2483 3197 4742 1132 Size: 97 1062 192 714 1545 2229 Fragments arranged by size: 2229 1545 1062 714 192 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5839 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2738 2770 3086 3118 3908 3940 5421 5453 2738 Size: 32 316 32 790 32 1481 32 3124 Fragments arranged by size: 3124 1481 790 316 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 2242 Size: 5839 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PstI C_TGCA'G Cuts at: 830 2685 3191 830 Size: 1855 506 3478 Fragments arranged by size: 3478 1855 506 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 PvuII CAG'CTG Cuts at: 2380 2524 2965 2380 Size: 144 441 5254 Fragments arranged by size: 5254 441 144 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SapI GCTCTTCn'nnn_ Cuts at: 2063 2063 Size: 5839 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 2687 4087 5799 2687 Size: 1400 1712 2727 Fragments arranged by size: 2727 1712 1400 SexAI A'CCwGG_T Cuts at: 2244 2616 2244 Size: 372 5467 SfcI C'TryA_G Cuts at: 826 1045 2199 2681 3187 4322 5003 5194 826 Size: 219 1154 482 506 1135 681 191 1471 Fragments arranged by size: 1471 1154 1135 681 506 482 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 3166 1872 Size: 1294 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3122 3943 4844 5106 820 Size: 197 926 1179 821 901 262 1553 Fragments arranged by size: 1553 1179 926 901 821 262 197 SnaBI TAC'GTA Cuts at: 493 3136 3630 493 Size: 2643 494 2702 Fragments arranged by size: 2702 2643 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 2291 3197 1132 Size: 1159 906 3774 Fragments arranged by size: 3774 1159 906 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 2933 1517 Size: 1416 4423 StyI C'CwwG_G Cuts at: 513 1065 2022 3278 513 Size: 552 957 1256 3074 Fragments arranged by size: 3074 1256 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3040 3866 3883 4036 4221 4666 325 Size: 1255 1460 826 17 153 185 445 1498 Fragments arranged by size: 1498 1460 1255 826 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2304 2685 4085 Size: 274 80 33 51 1443 327 381 1400 Cuts at: 4085 5797 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1400 381 327 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5799 5831 5799 Size: 32 5807 Tth111I GACn'n_nGTC Cuts at: 1316 2246 1316 Size: 930 4909 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2460 2460 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 3164 1870 Size: 1294 4545 XmnI GAAnn'nnTTC Cuts at: 2507 2753 3968 2507 Size: 246 1215 4378 Fragments arranged by size: 4378 1215 246 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI