Restriction map for
pFN21ASDA0048
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbsI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BpuAI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI MaeI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1593 GACGTC GACGT^C
AccI 1039 GTCTAC GT^[AC][GT]AC
Aor51HI 1754 AGCGCT AGC^GCT
ApaI 656 GGGCCC GGGCC^C
AvaI 1465 CCCGGG C^[CT]CG[GA]G
BbeI 1478 GGCGCC GGCGC^C
BbrPI 1494 CACGTG CAC^GTG
BclI 335 TGATCA T^GATCA
BglI 1258 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1494 CACGTG [CT]AC^GT[GA]
BsaBI 444 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1057 TCCGGT [AT]^CCGG[AT]
BsmBI 775 GAGACG CGTCTC(1/5)
BsmI 1251 GAATGC GAATGC(1/-1)
BsoBI 1465 CCCGGG C^[CT]CG[GA]G
BspHI 1760 TCATGA T^CATGA
BspLU11I 100 ACATGT A^CATGT
BssSI 634 CTCGTG CACGAG(-5/-1)
BstXI 1155 CCATCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1679 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1448 TGGCCG [CT]^GGCC[GA]
Eco47III 1754 AGCGCT AGC^GCT
EcoNI 1077 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 896 CCAAGG C^C[AT][AT]GG
FbaI 335 TGATCA T^GATCA
KasI 1478 GGCGCC G^GCGCC
MamI 444 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1478 GGCGCC GG^CGCC
PmaCI 1494 CACGTG CAC^GTG
PmlI 1494 CACGTG CAC^GTG
PpuMI 346 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1760 TCATGA T^CATGA
SacI 1690 GAGCTC GAGCT^C
SapI 726 GAAGAGC GCTCTTC(1/4)
SexAI 1799 ACCTGGT A^CC[AT]GGT
SfoI 1478 GGCGCC GGC^GCC
SmaI 1465 CCCGGG CCC^GGG
SphI 1701 GCATGC GCATG^C
StyI 896 CCAAGG C^C[AT][AT]GG
XcmI 447 CCAGATCATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1465 CCCGGG C^CCGGG
XmaI 1465 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 170 CGCG CG^CG
798 CGCG
AcyI 1478 GGCGCC G[GA]^CG[CT]C
1593 GACGTC
AflIII 100 ACATGT A^C[GA][CT]GT
1495 ACGTGT
AlwNI 934 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1357 CAGCAGCTG
Asp700I 1668 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1752 GAAGCGCTTC
AspHI 1148 GTGCTC G[AT]GC[AT]^C
1690 GAGCTC
BsaHI 1478 GGCGCC G[GA]^CG[CT]C
1593 GACGTC
BsaI 790 GAGACC GGTCTC(1/5)
1456 GAGACC
BseRI 670 CTCCTC GAGGAG(10/8)
1764 GAGGAG
BsiHKAI 1148 GTGCTC G[AT]GC[AT]^C
1690 GAGCTC
BsrBI 564 GAGCGG CCGCTC(-3/-3)
1633 GAGCGG
BsrDI 602 GCAATG GCAATG(2/0)
1282 GCAATG
BstUI 170 CGCG CG^CG
798 CGCG
DsaI 169 CCGCGG C^C[GA][CT]GG
797 CCGCGG
HgaI 609 GACGC GACGC(5/10)
1206 GCGTC
Hin1I 1478 GGCGCC G[GA]^CG[CT]C
1593 GACGTC
HincII 585 GTTGAC GT[CT]^[GA]AC
625 GTCAAC
HindII 585 GTTGAC GT[CT]^[GA]AC
625 GTCAAC
KspI 169 CCGCGG CCGC^GG
797 CCGCGG
MslI 552 CACCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1156 CATCTGAGTG
MvnI 170 CGCG CG^CG
798 CGCG
NspI 100 ACATGT [GA]CATG^[CT]
1701 GCATGC
PleI 64 GACTC GAGTC(4/5)
21 GAGTC
PstI 37 CTGCAG CTGCA^G
1106 CTGCAG
SacII 169 CCGCGG CCGC^GG
797 CCGCGG
XmnI 1668 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1752 GAAGCGCTTC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII