(Circular) (Six-base) MAPSORT of: pFN21ASDA0048.seq Check: 1532 from: 1 to: 6547 pFN21ASDA0048 6547 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2782 2926 2782 Size: 144 6403 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3620 4357 278 Size: 53 83 186 936 2084 737 2468 Fragments arranged by size: 2468 2084 936 737 186 83 53 AccI GT'mk_AC Cuts at: 1937 3063 3890 1937 Size: 1126 827 4594 Fragments arranged by size: 4594 1126 827 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 4610 5994 6262 1632 Size: 635 2343 1384 268 1917 Fragments arranged by size: 2343 1917 1384 635 268 AfeI AGC'GCT Cuts at: 3779 3779 Size: 6547 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6350 AflIII A'CryG_T Cuts at: 1203 2123 3518 5446 6423 1203 Size: 920 1395 1928 977 1327 Fragments arranged by size: 1928 1395 1327 977 920 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 5276 5276 Size: 6547 AleI CACnn'nnGTG Cuts at: 1166 2579 1166 Size: 1413 5134 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3225 1184 Size: 2041 4506 AlwNI CAG_nnn'CTG Cuts at: 2962 3385 4290 5862 2962 Size: 423 905 1572 3647 Fragments arranged by size: 3647 1572 905 423 ApaI G_GGCC'C Cuts at: 2683 2683 Size: 6547 ApaLI G'TGCA_C Cuts at: 4604 5760 4604 Size: 1156 5391 ApoI r'AATT_y Cuts at: 1493 3856 4082 4234 6343 1493 Size: 2363 226 152 2109 1697 Fragments arranged by size: 2363 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 3488 3872 1870 Size: 73 1545 384 4545 Fragments arranged by size: 4545 1545 384 73 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2483 2911 3501 3868 5323 Size: 325 217 627 696 428 590 367 1455 Cuts at: 5323 618 Size: 1842 Fragments arranged by size: 1842 1455 696 627 590 428 367 325 217 BanII G_rGCy'C Cuts at: 1391 2683 2870 3437 3717 3866 1391 Size: 1292 187 567 280 149 4072 Fragments arranged by size: 4072 1292 567 280 187 149 BbeI G_GCGC'C Cuts at: 3505 3505 Size: 6547 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5576 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4738 4772 1903 Size: 34 2801 34 3678 Fragments arranged by size: 3678 2801 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4439 5649 4439 Size: 1210 5337 BclI T'GATC_A Cuts at: 1548 2358 1548 Size: 810 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3287 5158 136 Size: 107 122 71 631 605 1615 1871 1525 Fragments arranged by size: 1871 1615 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6542 6542 Size: 6547 BlpI GC'TnA_GC Cuts at: 2787 3042 3431 3964 2787 Size: 255 389 533 5370 Fragments arranged by size: 5370 533 389 255 Bme1580I G_kGCm'C Cuts at: 1790 2683 2916 3634 4608 5764 1790 Size: 893 233 718 974 1156 2573 Fragments arranged by size: 2573 1156 974 893 718 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2043 3277 3760 Size: 416 53 83 399 24 626 1234 483 Cuts at: 3760 4278 5236 442 Size: 518 958 1753 Fragments arranged by size: 1753 1234 958 626 518 483 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 2340 3573 5207 1239 Size: 888 213 1233 1634 2579 Fragments arranged by size: 2579 1634 1233 888 213 Bpu10I CC'TnA_GC Cuts at: 2104 2104 Size: 6547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4672 5537 5835 4672 Size: 865 298 5384 Fragments arranged by size: 5384 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2807 3473 5210 882 Size: 617 1308 666 1737 2219 Fragments arranged by size: 2219 1737 1308 666 617 BsaAI yAC'GTr Cuts at: 493 1204 3519 3844 4338 493 Size: 711 2315 325 494 2702 Fragments arranged by size: 2702 2315 711 494 325 BsaBI GATnn'nnATC Cuts at: 2471 6541 2471 Size: 4070 2477 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 3617 4354 4736 Size: 53 83 186 936 1969 115 737 382 Cuts at: 4736 5371 275 Size: 635 1451 Fragments arranged by size: 1969 1451 936 737 635 382 186 115 83 53 BsaWI w'CCGG_w Cuts at: 3080 4980 5378 5652 5799 6210 3080 Size: 1900 398 274 147 411 3417 Fragments arranged by size: 3417 1900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3533 3563 1178 Size: 30 165 30 2130 30 4162 Fragments arranged by size: 4162 2130 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2684 3802 1186 Size: 1498 1118 3931 Fragments arranged by size: 3931 1498 1118 BseYI C'CCAG_C Cuts at: 2091 2766 2952 3118 3250 3483 5750 6246 2091 Size: 675 186 166 132 233 2267 496 2392 Fragments arranged by size: 2392 2267 675 496 233 186 166 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2603 2993 3110 3413 3499 3629 1163 Size: 573 867 390 117 303 86 130 4081 Fragments arranged by size: 4081 867 573 390 303 130 117 86 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3997 4758 5786 1540 Size: 275 186 1996 761 1028 2301 Fragments arranged by size: 2301 1996 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3175 3717 4608 4693 5764 3175 Size: 542 891 85 1071 3958 Fragments arranged by size: 3958 1071 891 542 85 BsmI GAATG_Cn' Cuts at: 3280 4058 4151 3280 Size: 778 93 5676 Fragments arranged by size: 5676 778 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2792 2792 Size: 6547 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2683 2870 2916 3175 3437 3634 3717 Size: 399 893 187 46 259 262 197 83 Cuts at: 3717 3866 4608 4693 5764 1391 Size: 149 742 85 1071 2174 Fragments arranged by size: 2174 1071 893 742 399 262 259 197 187 149 85 83 46 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 3783 4436 6166 3783 Size: 653 1730 4164 Fragments arranged by size: 4164 1730 653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 2224 2782 2859 2890 2926 3902 844 Size: 1223 157 558 77 31 36 976 3489 Fragments arranged by size: 3489 1223 976 558 157 77 36 31 BsrBI CCG'CTC Cuts at: 1394 1823 2589 3658 4434 1394 Size: 429 766 1069 776 3507 Fragments arranged by size: 3507 1069 776 766 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2632 3312 5042 5216 65 Size: 1183 1384 680 1730 174 1396 Fragments arranged by size: 1730 1396 1384 1183 680 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5191 5378 1700 Size: 110 3381 187 2869 Fragments arranged by size: 3381 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 2657 4607 5619 2657 Size: 1950 1012 3585 Fragments arranged by size: 3585 1950 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2962 3128 3170 3513 794 Size: 2168 166 42 343 3828 Fragments arranged by size: 3828 2168 343 166 42 BstBI TT'CG_AA Cuts at: 2007 5351 2007 Size: 3344 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6547 BstXI CCAn_nnnn'nTGG Cuts at: 3185 3185 Size: 6547 BstYI r'GATC_y Cuts at: 1972 2466 2946 3030 3444 3877 4244 4629 4646 Size: 494 480 84 414 433 367 385 17 Cuts at: 4646 6098 6184 6196 6542 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 494 480 433 414 385 367 346 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2192 2820 513 Size: 552 957 170 628 4240 Fragments arranged by size: 4240 957 628 552 170 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5808 Fragments arranged by size: 5808 683 56 BtsI GCAGTG_nn' Cuts at: 2031 4063 4857 4877 2031 Size: 2032 794 20 3701 Fragments arranged by size: 3701 2032 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6512 DraI TTT'AAA Cuts at: 3851 3851 Size: 6547 DraIII CAC_nnn'GTG Cuts at: 3707 3707 Size: 6547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3471 3994 4883 Size: 54 1425 50 275 18 1641 523 889 Cuts at: 4883 6512 8 Size: 1629 43 Fragments arranged by size: 1641 1629 1425 889 523 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3994 1537 Size: 275 2182 4090 Fragments arranged by size: 4090 2182 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2744 3539 3686 4477 1353 Size: 1391 795 147 791 3423 Fragments arranged by size: 3423 1391 795 791 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5134 5508 5654 387 Size: 1064 138 3545 374 146 1280 Fragments arranged by size: 3545 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3715 3715 Size: 6547 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2267 2340 3573 4610 5207 5994 Size: 393 495 140 73 1233 1037 597 787 Cuts at: 5994 6262 1239 Size: 268 1524 Fragments arranged by size: 1524 1233 1037 787 597 495 393 268 140 73 EcoNI CCTnn'n_nnAGG Cuts at: 3104 3104 Size: 6547 EcoO109I rG'GnC_Cy Cuts at: 2370 2679 2680 2370 Size: 309 1 6237 Fragments arranged by size: 6237 309 1 EcoRI G'AATT_C Cuts at: 3856 4234 3856 Size: 378 6169 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2266 3637 2266 Size: 1371 5176 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 2310 3505 3781 5694 1678 Size: 632 1195 276 1913 2531 Fragments arranged by size: 2531 1913 1195 632 276 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2623 2655 3040 3072 3533 Size: 32 135 32 1051 32 385 32 461 Cuts at: 3533 3565 5188 5220 5262 5294 1373 Size: 32 1623 32 42 32 2626 Fragments arranged by size: 2626 1623 1051 461 385 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2610 2650 3891 4137 669 Size: 988 281 672 40 1241 246 3079 Fragments arranged by size: 3079 1241 988 672 281 246 40 HindIII A'AGCT_T Cuts at: 748 748 Size: 6547 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KasI G'GCGC_C Cuts at: 3501 3501 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3742 5661 5845 1305 Size: 2437 1919 184 2007 Fragments arranged by size: 2437 2007 1919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4725 Fragments arranged by size: 4725 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2579 3183 4505 4864 5023 518 Size: 648 1413 604 1322 359 159 2042 Fragments arranged by size: 2042 1413 1322 648 604 359 159 MspA1I CmG'CkG Cuts at: 1606 2194 2329 2822 2888 3041 3128 3209 3296 Size: 588 135 493 66 153 87 81 87 Cuts at: 3296 3385 3451 3963 4640 5788 6033 1606 Size: 89 66 512 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 588 512 493 245 153 135 89 87 87 81 66 66 NarI GG'CG_CC Cuts at: 3502 3502 Size: 6547 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5038 Fragments arranged by size: 5038 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 2127 3728 3905 5450 1132 Size: 97 898 1601 177 1545 2229 Fragments arranged by size: 2229 1601 1545 898 177 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6547 PciI A'CATG_T Cuts at: 2123 5446 2123 Size: 3323 3224 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6547 PfoI T'CCnGG_A Cuts at: 2317 3105 2317 Size: 788 5759 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PmlI CAC'GTG Cuts at: 3519 3519 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2370 2370 Size: 6547 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspOMI G'GGCC_C Cuts at: 2679 2679 Size: 6547 PstI C_TGCA'G Cuts at: 830 2064 3133 3899 830 Size: 1234 1069 766 3478 Fragments arranged by size: 3478 1234 1069 766 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 PvuII CAG'CTG Cuts at: 2329 2888 3041 3128 3209 3296 3385 3451 2329 Size: 559 153 87 81 87 89 66 5425 Fragments arranged by size: 5425 559 153 89 87 87 81 66 SacI G_AGCT'C Cuts at: 3717 3717 Size: 6547 SacII CC_GC'GG Cuts at: 2195 2823 2195 Size: 628 5919 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SanDI GG'GwC_CC Cuts at: 2370 2370 Size: 6547 SapI GCTCTTCn'nnn_ Cuts at: 2744 2744 Size: 6547 SbfI CC_TGCA'GG Cuts at: 3899 3899 Size: 6547 ScaI AGT'ACT Cuts at: 4795 6507 4795 Size: 1712 4835 SexAI A'CCwGG_T Cuts at: 3822 3822 Size: 6547 SfcI C'TryA_G Cuts at: 826 1045 2060 3129 3151 3895 5030 5711 5902 Size: 219 1015 1069 22 744 1135 681 191 Cuts at: 5902 826 Size: 1471 Fragments arranged by size: 1471 1135 1069 1015 744 681 219 191 22 SfoI GGC'GCC Cuts at: 3503 3503 Size: 6547 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 3490 3874 1872 Size: 1618 384 4545 Fragments arranged by size: 4545 1618 384 SmlI C'TyrA_G Cuts at: 820 1017 1943 4651 5552 5814 820 Size: 197 926 2708 901 262 1553 Fragments arranged by size: 2708 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3844 4338 493 Size: 3351 494 2702 Fragments arranged by size: 3351 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 3728 3905 1132 Size: 2596 177 3774 Fragments arranged by size: 3774 2596 177 SspI AAT'ATT Cuts at: 5 52 4471 5 Size: 47 4419 2081 Fragments arranged by size: 4419 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6547 StyI C'CwwG_G Cuts at: 513 1065 2022 2919 3986 513 Size: 552 957 897 1067 3074 Fragments arranged by size: 3074 1067 957 897 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2096 3455 3749 4574 4591 4744 4929 Size: 1255 516 1359 294 825 17 153 185 Cuts at: 4929 5374 325 Size: 445 1498 Fragments arranged by size: 1498 1359 1255 825 516 445 294 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2443 4793 6505 Size: 274 80 33 51 1443 466 2350 1712 Cuts at: 6505 96 Size: 138 Fragments arranged by size: 2350 1712 1443 466 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6507 6539 6507 Size: 32 6515 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6547 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2477 2477 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6547 XmaI C'CCGG_G Cuts at: 1870 3488 3872 1870 Size: 1618 384 4545 Fragments arranged by size: 4545 1618 384 XmnI GAAnn'nnTTC Cuts at: 3695 3779 4676 3695 Size: 84 897 5566 Fragments arranged by size: 5566 897 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3618 4355 276 Size: 53 83 186 936 2084 737 2468 Fragments arranged by size: 2468 2084 936 737 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SfiI SrfI SwaI