Restriction map for
pFN21ASDA0048
(Linear) (Six-base) MAPSORT of: KIAA0048.seq Check: 8933 from: 1 to: 1818
KIAA0048.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 759 903 1818
Size: 759 144 915
Fragments arranged by size:
915 759 144
AatII G_ACGT'C
Cuts at: 0 1597 1818
Size: 1597 221
AccI GT'mk_AC
Cuts at: 0 1040 1818
Size: 1040 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 1818
Size: 244 1574
AfeI AGC'GCT
Cuts at: 0 1756 1818
Size: 1756 62
AflIII A'CryG_T
Cuts at: 0 100 1495 1818
Size: 100 1395 323
Fragments arranged by size:
1395 323 100
AleI CACnn'nnGTG
Cuts at: 0 556 1818
Size: 556 1262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1202 1818
Size: 1202 616
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1362 1818
Size: 939 423 456
Fragments arranged by size:
939 456 423
ApaI G_GGCC'C
Cuts at: 0 660 1818
Size: 660 1158
AvaI C'yCGr_G
Cuts at: 0 1465 1818
Size: 1465 353
BanI G'GyrC_C
Cuts at: 0 460 888 1478 1818
Size: 460 428 590 340
Fragments arranged by size:
590 460 428 340
BanII G_rGCy'C
Cuts at: 0 660 847 1414 1694 1818
Size: 660 187 567 280 124
Fragments arranged by size:
660 567 280 187 124
BbeI G_GCGC'C
Cuts at: 0 1482 1818
Size: 1482 336
BclI T'GATC_A
Cuts at: 0 335 1818
Size: 335 1483
BglI GCCn_nnn'nGGC
Cuts at: 0 1264 1818
Size: 1264 554
BlpI GC'TnA_GC
Cuts at: 0 764 1019 1408 1818
Size: 764 255 389 410
Fragments arranged by size:
764 410 389 255
Bme1580I G_kGCm'C
Cuts at: 0 660 893 1611 1818
Size: 660 233 718 207
Fragments arranged by size:
718 660 233 207
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 1254 1737 1818
Size: 20 1234 483 81
Fragments arranged by size:
1234 483 81 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 317 1550 1818
Size: 104 213 1233 268
Fragments arranged by size:
1233 268 213 104
Bpu10I CC'TnA_GC
Cuts at: 0 81 1818
Size: 81 1737
BsaI GGTCTCn'nnnn_
Cuts at: 0 784 1450 1818
Size: 784 666 368
Fragments arranged by size:
784 666 368
BsaAI yAC'GTr
Cuts at: 0 1496 1818
Size: 1496 322
BsaBI GATnn'nnATC
Cuts at: 0 448 1818
Size: 448 1370
BsaHI Gr'CG_yC
Cuts at: 0 1479 1594 1818
Size: 1479 115 224
Fragments arranged by size:
1479 224 115
BsaWI w'CCGG_w
Cuts at: 0 1057 1818
Size: 1057 761
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1510 1540 1818
Size: 1510 30 278
Fragments arranged by size:
1510 278 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 1779 1818
Size: 661 1118 39
Fragments arranged by size:
1118 661 39
BseYI C'CCAG_C
Cuts at: 0 68 743 929 1095 1227 1460 1818
Size: 68 675 186 166 132 233 358
Fragments arranged by size:
675 358 233 186 166 132 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 970 1087 1390 1476 1606 1818
Size: 580 390 117 303 86 130 212
Fragments arranged by size:
580 390 303 212 130 117 86
BsiHKAI G_wGCw'C
Cuts at: 0 1152 1694 1818
Size: 1152 542 124
Fragments arranged by size:
1152 542 124
BsmI GAATG_Cn'
Cuts at: 0 1257 1818
Size: 1257 561
BsmBI CGTCTCn'nnnn_
Cuts at: 0 769 1818
Size: 769 1049
Bsp1286I G_dGCh'C
Cuts at: 0 660 847 893 1152 1414 1611 1694 1818
Size: 660 187 46 259 262 197 83 124
Fragments arranged by size:
660 262 259 197 187 124 83 46
BspHI T'CATG_A
Cuts at: 0 1760 1818
Size: 1760 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 44 201 759 836 867 903 1818
Size: 44 157 558 77 31 36 915
Fragments arranged by size:
915 558 157 77 44 36 31
BsrBI CCG'CTC
Cuts at: 0 566 1635 1818
Size: 566 1069 183
Fragments arranged by size:
1069 566 183
BsrDI GCAATG_nn'
Cuts at: 0 609 1289 1818
Size: 609 680 529
Fragments arranged by size:
680 609 529
BssSI C'ACGA_G
Cuts at: 0 634 1818
Size: 634 1184
BstAPI GCAn_nnn'nTGC
Cuts at: 0 939 1105 1147 1490 1818
Size: 939 166 42 343 328
Fragments arranged by size:
939 343 328 166 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1162 1818
Size: 1162 656
BstYI r'GATC_y
Cuts at: 0 443 923 1007 1421 1818
Size: 443 480 84 414 397
Fragments arranged by size:
480 443 414 397 84
BtgI C'CryG_G
Cuts at: 0 169 797 1818
Size: 169 628 1021
Fragments arranged by size:
1021 628 169
BtsI GCAGTG_nn'
Cuts at: 0 8 1818
Size: 8 1810
DraIII CAC_nnn'GTG
Cuts at: 0 1684 1818
Size: 1684 134
EaeI y'GGCC_r
Cuts at: 0 1448 1818
Size: 1448 370
EarI CTCTTCn'nnn_
Cuts at: 0 721 1516 1663 1818
Size: 721 795 147 155
Fragments arranged by size:
795 721 155 147
EcoICRI GAG'CTC
Cuts at: 0 1692 1818
Size: 1692 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 244 317 1550 1818
Size: 104 140 73 1233 268
Fragments arranged by size:
1233 268 140 104 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1081 1818
Size: 1081 737
EcoO109I rG'GnC_Cy
Cuts at: 0 347 656 657 1818
Size: 347 309 1 1161
Fragments arranged by size:
1161 347 309 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 243 1614 1818
Size: 243 1371 204
Fragments arranged by size:
1371 243 204
HaeII r_GCGC'y
Cuts at: 0 287 1482 1758 1818
Size: 287 1195 276 60
Fragments arranged by size:
1195 287 276 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 600 632 1017 1049 1510 1542 1818
Size: 600 32 385 32 461 32 276
Fragments arranged by size:
600 461 385 276 32 32 32
HincII GTy'rAC
Cuts at: 0 587 627 1818
Size: 587 40 1191
Fragments arranged by size:
1191 587 40
KasI G'GCGC_C
Cuts at: 0 1478 1818
Size: 1478 340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1719 1818
Size: 1719 99
MslI CAynn'nnrTG
Cuts at: 0 556 1160 1818
Size: 556 604 658
Fragments arranged by size:
658 604 556
MspA1I CmG'CkG
Cuts at: 0 171 306 799 865 1018 1105 1186 1273
Size: 171 135 493 66 153 87 81 87
Cuts at: 1273 1362 1428 1818
Size: 89 66 390
Fragments arranged by size:
493 390 171 153 135 89 87 87
81 66 66
NarI GG'CG_CC
Cuts at: 0 1479 1818
Size: 1479 339
NspI r_CATG'y
Cuts at: 0 104 1705 1818
Size: 104 1601 113
Fragments arranged by size:
1601 113 104
PciI A'CATG_T
Cuts at: 0 100 1818
Size: 100 1718
PfoI T'CCnGG_A
Cuts at: 0 294 1082 1818
Size: 294 788 736
Fragments arranged by size:
788 736 294
PmlI CAC'GTG
Cuts at: 0 1496 1818
Size: 1496 322
PpuMI rG'GwC_Cy
Cuts at: 0 347 1818
Size: 347 1471
PspOMI G'GGCC_C
Cuts at: 0 656 1818
Size: 656 1162
PstI C_TGCA'G
Cuts at: 0 41 1110 1818
Size: 41 1069 708
Fragments arranged by size:
1069 708 41
PvuII CAG'CTG
Cuts at: 0 306 865 1018 1105 1186 1273 1362 1428
Size: 306 559 153 87 81 87 89 66
Cuts at: 1428 1818
Size: 390
Fragments arranged by size:
559 390 306 153 89 87 87 81
66
SacI G_AGCT'C
Cuts at: 0 1694 1818
Size: 1694 124
SacII CC_GC'GG
Cuts at: 0 172 800 1818
Size: 172 628 1018
Fragments arranged by size:
1018 628 172
SanDI GG'GwC_CC
Cuts at: 0 347 1818
Size: 347 1471
SapI GCTCTTCn'nnn_
Cuts at: 0 721 1818
Size: 721 1097
SexAI A'CCwGG_T
Cuts at: 0 1799 1818
Size: 1799 19
SfcI C'TryA_G
Cuts at: 0 37 1106 1128 1818
Size: 37 1069 22 690
Fragments arranged by size:
1069 690 37 22
SfoI GGC'GCC
Cuts at: 0 1480 1818
Size: 1480 338
SmaI CCC'GGG
Cuts at: 0 1467 1818
Size: 1467 351
SphI G_CATG'C
Cuts at: 0 1705 1818
Size: 1705 113
StyI C'CwwG_G
Cuts at: 0 896 1818
Size: 896 922
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 1432 1726 1818
Size: 73 1359 294 92
Fragments arranged by size:
1359 294 92 73
TatI w'GTAC_w
Cuts at: 0 420 1818
Size: 420 1398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 454 1818
Size: 454 1364
XmaI C'CCGG_G
Cuts at: 0 1465 1818
Size: 1465 353
XmnI GAAnn'nnTTC
Cuts at: 0 1672 1756 1818
Size: 1672 84 62
Fragments arranged by size:
1672 84 62
ZraI GAC'GTC
Cuts at: 0 1595 1818
Size: 1595 223
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI AvaI BanI BanII BbeI BclI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI
BstXI BstYI BtgI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI
MmeI MslI MspA1I NarI NspI PciI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SexAI SfcI SfoI SmaI SphI StyI TaqII TatI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BfrBI BglII BmgBI BmtI BplI BpuEI
BsiEI BsiWI BspEI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI