(Circular) (Six-base) MAPSORT of: pFN21ASDA0046.seq Check: 7290 from: 1 to: 7336 pFN21ASDA0046 7336 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2049 4533 2049 Size: 2484 4852 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5146 278 Size: 53 83 186 936 3610 2468 Fragments arranged by size: 3610 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3858 4679 1937 Size: 1921 821 4594 Fragments arranged by size: 4594 1921 821 Acc65I G'GTAC_C Cuts at: 2801 4371 4657 2801 Size: 1570 286 5480 Fragments arranged by size: 5480 1570 286 AclI AA'CG_TT Cuts at: 1563 5463 5836 1563 Size: 3900 373 3063 Fragments arranged by size: 3900 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5399 6783 7051 1632 Size: 3767 1384 268 1917 Fragments arranged by size: 3767 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7139 AflIII A'CryG_T Cuts at: 1203 4086 6235 7212 1203 Size: 2883 2149 977 1327 Fragments arranged by size: 2883 2149 1327 977 AgeI A'CCGG_T Cuts at: 6167 6167 Size: 7336 AhdI GACnn_n'nnGTC Cuts at: 6065 6065 Size: 7336 AleI CACnn'nnGTG Cuts at: 1166 3182 1166 Size: 2016 5320 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3381 1184 Size: 2197 5139 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4211 4243 4211 Size: 32 7304 AlwNI CAG_nnn'CTG Cuts at: 3883 4150 5079 6651 3883 Size: 267 929 1572 4568 Fragments arranged by size: 4568 1572 929 267 ApaLI G'TGCA_C Cuts at: 3119 5393 6549 3119 Size: 2274 1156 3906 Fragments arranged by size: 3906 2274 1156 ApoI r'AATT_y Cuts at: 1493 3286 4589 4645 4871 5023 7132 1493 Size: 1793 1303 56 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 1303 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 5890 160 Size: 872 4858 1606 Fragments arranged by size: 4858 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7336 AvaI C'yCGr_G Cuts at: 1870 1943 2099 2501 4020 4661 1870 Size: 73 156 402 1519 641 4545 Fragments arranged by size: 4545 1519 641 402 156 73 BamHI G'GATC_C Cuts at: 4666 5033 4666 Size: 367 6969 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2533 2788 2801 4371 4657 Size: 325 217 627 746 255 13 1570 286 Cuts at: 4657 6112 618 Size: 1455 1842 Fragments arranged by size: 1842 1570 1455 746 627 325 286 255 217 13 BanII G_rGCy'C Cuts at: 1391 2607 2850 3687 3843 4655 1391 Size: 1216 243 837 156 812 4072 Fragments arranged by size: 4072 1216 837 812 243 156 BbeI G_GCGC'C Cuts at: 2792 2792 Size: 7336 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2437 4212 4483 928 Size: 971 538 1775 271 3781 Fragments arranged by size: 3781 1775 971 538 271 BbvCI CC'TCA_GC Cuts at: 4607 4607 Size: 7336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4498 4532 5527 5561 1903 Size: 34 2561 34 995 34 3678 Fragments arranged by size: 3678 2561 995 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2490 5228 6438 2490 Size: 2738 1210 3388 Fragments arranged by size: 3388 2738 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7336 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2391 3771 4352 Size: 107 122 71 631 605 719 1380 581 Cuts at: 4352 5947 136 Size: 1595 1525 Fragments arranged by size: 1595 1525 1380 719 631 605 581 122 107 71 BglII A'GATC_T Cuts at: 7331 7331 Size: 7336 BlpI GC'TnA_GC Cuts at: 4753 4753 Size: 7336 Bme1580I G_kGCm'C Cuts at: 1790 3123 5397 6553 1790 Size: 1333 2274 1156 2573 Fragments arranged by size: 2573 2274 1333 1156 BmgBI CAC'GTC Cuts at: 1318 4480 1318 Size: 3162 4174 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5067 6025 442 Size: 416 53 83 399 24 3650 958 1753 Fragments arranged by size: 3650 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7336 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2678 4182 2678 Size: 1504 5832 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2619 2787 3251 3359 5996 1239 Size: 1380 168 464 108 2637 2579 Fragments arranged by size: 2637 2579 1380 464 168 108 Bpu10I CC'TnA_GC Cuts at: 3132 4607 3132 Size: 1475 5861 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 5461 6326 6624 2330 Size: 3131 865 298 3042 Fragments arranged by size: 3131 3042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2046 2081 2213 2312 5999 882 Size: 617 547 35 132 99 3687 2219 Fragments arranged by size: 3687 2219 617 547 132 99 35 BsaAI yAC'GTr Cuts at: 493 1204 2853 3760 4633 5127 493 Size: 711 1649 907 873 494 2702 Fragments arranged by size: 2702 1649 907 873 711 494 BsaBI GATnn'nnATC Cuts at: 2181 7330 2181 Size: 5149 2187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2789 5143 5525 6160 Size: 53 83 186 936 1256 2354 382 635 Cuts at: 6160 275 Size: 1451 Fragments arranged by size: 2354 1451 1256 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2553 5769 6167 6441 6588 6999 2553 Size: 3216 398 274 147 411 2890 Fragments arranged by size: 3216 2890 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2813 2843 4211 4241 4539 Size: 30 165 30 1410 30 1368 30 298 Cuts at: 4539 4569 1178 Size: 30 3945 Fragments arranged by size: 3945 1410 1368 298 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2147 2150 2390 2834 4127 4235 4305 Size: 931 30 3 240 444 1293 108 70 Cuts at: 4305 1186 Size: 4217 Fragments arranged by size: 4217 1293 931 444 240 108 70 30 3 BseYI C'CCAG_C Cuts at: 2928 6539 7035 2928 Size: 3611 496 3229 Fragments arranged by size: 3611 3229 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2053 2275 2401 3618 1163 Size: 573 317 222 126 1217 4881 Fragments arranged by size: 4881 1217 573 317 222 126 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4265 4786 5547 6575 1540 Size: 275 186 2264 521 761 1028 2301 Fragments arranged by size: 2301 2264 1028 761 521 275 186 BsiHKAI G_wGCw'C Cuts at: 2865 2981 3123 4101 5397 5482 6553 2865 Size: 116 142 978 1296 85 1071 3648 Fragments arranged by size: 3648 1296 1071 978 142 116 85 BsmI GAATG_Cn' Cuts at: 3221 4847 4940 3221 Size: 1626 93 5617 Fragments arranged by size: 5617 1626 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2555 4468 4486 2555 Size: 1913 18 5405 Fragments arranged by size: 5405 1913 18 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2607 2850 2865 2981 3123 3687 3843 Size: 399 817 243 15 116 142 564 156 Cuts at: 3843 4101 4655 5397 5482 6553 1391 Size: 258 554 742 85 1071 2174 Fragments arranged by size: 2174 1071 817 742 564 554 399 258 243 156 142 116 85 15 BspEI T'CCGG_A Cuts at: 2553 6999 2553 Size: 4446 2890 BspHI T'CATG_A Cuts at: 3960 5225 6955 3960 Size: 1265 1730 4341 Fragments arranged by size: 4341 1730 1265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 2477 4533 4691 844 Size: 1205 428 2056 158 3489 Fragments arranged by size: 3489 2056 1205 428 158 BsrBI CCG'CTC Cuts at: 1394 1823 2759 4225 5223 1394 Size: 429 936 1466 998 3507 Fragments arranged by size: 3507 1466 998 936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5831 6005 65 Size: 1183 4583 174 1396 Fragments arranged by size: 4583 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2476 5980 6167 1700 Size: 110 666 3504 187 2869 Fragments arranged by size: 3504 2869 666 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7336 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7336 BssSI C'ACGA_G Cuts at: 5396 6408 5396 Size: 1012 6324 BstAPI GCAn_nnn'nTGC Cuts at: 794 2064 4147 794 Size: 1270 2083 3983 Fragments arranged by size: 3983 2083 1270 BstBI TT'CG_AA Cuts at: 2007 6140 2007 Size: 4133 3203 BstEII G'GTnAC_C Cuts at: 1184 3172 1184 Size: 1988 5348 BstXI CCAn_nnnn'nTGG Cuts at: 2948 3184 3392 2948 Size: 236 208 6892 Fragments arranged by size: 6892 236 208 BstYI r'GATC_y Cuts at: 1972 2694 4666 5033 5418 5435 6887 6973 6985 Size: 722 1972 367 385 17 1452 86 12 Cuts at: 6985 7331 1972 Size: 346 1977 Fragments arranged by size: 1977 1972 1452 722 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2223 3180 3903 3940 4215 4246 Size: 552 957 201 957 723 37 275 31 Cuts at: 4246 4367 4530 513 Size: 121 163 3319 Fragments arranged by size: 3319 957 957 723 552 275 201 163 121 37 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4087 487 Size: 683 56 2861 3736 Fragments arranged by size: 3736 2861 683 56 BtsI GCAGTG_nn' Cuts at: 4852 5646 5666 4852 Size: 794 20 6522 Fragments arranged by size: 6522 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7336 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7301 DraI TTT'AAA Cuts at: 4640 4640 Size: 7336 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6343 809 Size: 507 5027 1802 Fragments arranged by size: 5027 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3336 3711 4353 Size: 54 1425 50 275 18 1506 375 642 Cuts at: 4353 4783 5672 7301 8 Size: 430 889 1629 43 Fragments arranged by size: 1629 1506 1425 889 642 430 375 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4783 1537 Size: 275 2971 4090 Fragments arranged by size: 4090 2971 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 2612 3238 3350 3386 5266 1353 Size: 986 273 626 112 36 1880 3423 Fragments arranged by size: 3423 1880 986 626 273 112 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4220 5923 6297 6443 387 Size: 1064 138 2631 1703 374 146 1280 Fragments arranged by size: 2631 1703 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2619 2787 3251 3359 5399 5996 6783 Size: 393 987 168 464 108 2040 597 787 Cuts at: 6783 7051 1239 Size: 268 1524 Fragments arranged by size: 2040 1524 987 787 597 464 393 268 168 108 EcoO109I rG'GnC_Cy Cuts at: 2572 2624 3055 3579 4132 2572 Size: 52 431 524 553 5776 Fragments arranged by size: 5776 553 524 431 52 EcoRI G'AATT_C Cuts at: 3286 4645 5023 3286 Size: 1359 378 5599 Fragments arranged by size: 5599 1359 378 EcoRV GAT'ATC Cuts at: 3754 3754 Size: 7336 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2758 2758 Size: 7336 FspI TGC'GCA Cuts at: 3899 5842 3899 Size: 1943 5393 HaeII r_GCGC'y Cuts at: 1678 2792 4323 6483 1678 Size: 1114 1531 2160 2531 Fragments arranged by size: 2531 2160 1531 1114 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2732 2764 2813 2845 5977 Size: 32 135 32 1160 32 49 32 3132 Cuts at: 5977 6009 6051 6083 1373 Size: 32 42 32 2626 Fragments arranged by size: 3132 2626 1160 135 49 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4680 4926 669 Size: 988 281 2742 246 3079 Fragments arranged by size: 3079 2742 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7336 HpaI GTT'AAC Cuts at: 4926 4926 Size: 7336 KasI G'GCGC_C Cuts at: 2788 2788 Size: 7336 KpnI G_GTAC'C Cuts at: 2805 4375 4661 2805 Size: 1570 286 5480 Fragments arranged by size: 5480 1570 286 MfeI C'AATT_G Cuts at: 4935 4935 Size: 7336 MluI A'CGCG_T Cuts at: 7212 7212 Size: 7336 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3434 6450 6634 1305 Size: 2129 3016 184 2007 Fragments arranged by size: 3016 2129 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3338 3713 10 Size: 54 1425 343 1506 375 3633 Fragments arranged by size: 3633 1506 1425 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3182 3390 3704 4421 5294 5653 5812 Size: 648 2016 208 314 717 873 359 159 Cuts at: 5812 518 Size: 2042 Fragments arranged by size: 2042 2016 873 717 648 359 314 208 159 MspA1I CmG'CkG Cuts at: 1606 3602 3942 4369 4752 5429 6577 6822 1606 Size: 1996 340 427 383 677 1148 245 2120 Fragments arranged by size: 2120 1996 1148 677 427 383 340 245 NarI GG'CG_CC Cuts at: 2789 2789 Size: 7336 NcoI C'CATG_G Cuts at: 513 1065 2022 2223 4215 513 Size: 552 957 201 1992 3634 Fragments arranged by size: 3634 1992 957 552 201 NdeI CA'TA_TG Cuts at: 387 387 Size: 7336 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7336 NotI GC'GGCC_GC Cuts at: 4783 4783 Size: 7336 NspI r_CATG'y Cuts at: 1132 1229 3453 4090 4694 6239 1132 Size: 97 2224 637 604 1545 2229 Fragments arranged by size: 2229 2224 1545 637 604 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7336 PciI A'CATG_T Cuts at: 4086 6235 4086 Size: 2149 5187 PflMI CCAn_nnn'nTGG Cuts at: 1108 3883 4390 1108 Size: 2775 507 4054 Fragments arranged by size: 4054 2775 507 PfoI T'CCnGG_A Cuts at: 2492 2492 Size: 7336 PmeI GTTT'AAAC Cuts at: 4640 4640 Size: 7336 PmlI CAC'GTG Cuts at: 2853 3760 2853 Size: 907 6429 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 4211 4243 5405 5437 6918 6950 3941 Size: 32 238 32 1162 32 1481 32 4327 Fragments arranged by size: 4327 1481 1162 238 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2572 3579 4132 2572 Size: 1007 553 5776 Fragments arranged by size: 5776 1007 553 PshAI GACnn'nnGTC Cuts at: 1613 3226 3854 1613 Size: 1613 628 5095 Fragments arranged by size: 5095 1613 628 PsiI TTA'TAA Cuts at: 4906 4906 Size: 7336 PstI C_TGCA'G Cuts at: 830 2060 2779 4143 4599 4688 830 Size: 1230 719 1364 456 89 3478 Fragments arranged by size: 3478 1364 1230 719 456 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7336 SacII CC_GC'GG Cuts at: 3943 4370 3943 Size: 427 6909 SalI G'TCGA_C Cuts at: 1936 4678 1936 Size: 2742 4594 SapI GCTCTTCn'nnn_ Cuts at: 2612 3386 2612 Size: 774 6562 SbfI CC_TGCA'GG Cuts at: 4688 4688 Size: 7336 ScaI AGT'ACT Cuts at: 5584 7296 5584 Size: 1712 5624 SfcI C'TryA_G Cuts at: 826 1045 2056 2775 4139 4595 4684 5819 6500 Size: 219 1011 719 1364 456 89 1135 681 Cuts at: 6500 6691 826 Size: 191 1471 Fragments arranged by size: 1471 1364 1135 1011 719 681 456 219 191 89 SfoI GGC'GCC Cuts at: 2790 2790 Size: 7336 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7336 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7336 SmaI CCC'GGG Cuts at: 1872 4022 4663 1872 Size: 2150 641 4545 Fragments arranged by size: 4545 2150 641 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 5440 6341 6603 820 Size: 197 926 402 3095 901 262 1553 Fragments arranged by size: 3095 1553 926 901 402 262 197 SnaBI TAC'GTA Cuts at: 493 4633 5127 493 Size: 4140 494 2702 Fragments arranged by size: 4140 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7336 SphI G_CATG'C Cuts at: 1132 4694 1132 Size: 3562 3774 SspI AAT'ATT Cuts at: 5 52 5260 5 Size: 47 5208 2081 Fragments arranged by size: 5208 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7336 StyI C'CwwG_G Cuts at: 513 1065 2022 2116 2153 2223 2575 2842 4215 Size: 552 957 94 37 70 352 267 1373 Cuts at: 4215 4775 513 Size: 560 3074 Fragments arranged by size: 3074 1373 957 560 552 352 267 94 70 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 4251 4490 5363 5380 5533 5718 Size: 1255 774 1897 239 873 17 153 185 Cuts at: 5718 6163 325 Size: 445 1498 Fragments arranged by size: 1897 1498 1255 873 774 445 239 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5582 7294 96 Size: 274 80 33 51 1443 3605 1712 138 Fragments arranged by size: 3605 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4075 4107 4539 4571 7296 7328 4075 Size: 32 432 32 2725 32 4083 Fragments arranged by size: 4083 2725 432 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7336 XbaI T'CTAG_A Cuts at: 2815 4672 2815 Size: 1857 5479 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3832 4206 3832 Size: 374 6962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7336 XmaI C'CCGG_G Cuts at: 1870 4020 4661 1870 Size: 2150 641 4545 Fragments arranged by size: 4545 2150 641 XmnI GAAnn'nnTTC Cuts at: 3956 5465 3956 Size: 1509 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5144 276 Size: 53 83 186 936 3610 2468 Fragments arranged by size: 3610 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI