Restriction map for
pFN21ASDA0046
(Linear) (Six-base) MAPSORT of: KIAA0046.seq Check: 178 from: 1 to: 2607
KIAA0046.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 26 2510 2607
Size: 26 2484 97
Fragments arranged by size:
2484 97 26
AccI GT'mk_AC
Cuts at: 0 1835 2607
Size: 1835 772
Acc65I G'GTAC_C
Cuts at: 0 778 2348 2607
Size: 778 1570 259
Fragments arranged by size:
1570 778 259
AflIII A'CryG_T
Cuts at: 0 2063 2607
Size: 2063 544
AleI CACnn'nnGTG
Cuts at: 0 1159 2607
Size: 1159 1448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1358 2607
Size: 1358 1249
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2188 2220 2607
Size: 2188 32 387
Fragments arranged by size:
2188 387 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1860 2127 2607
Size: 1860 267 480
Fragments arranged by size:
1860 480 267
ApaLI G'TGCA_C
Cuts at: 0 1096 2607
Size: 1096 1511
ApoI r'AATT_y
Cuts at: 0 1263 2566 2607
Size: 1263 1303 41
Fragments arranged by size:
1303 1263 41
AvaI C'yCGr_G
Cuts at: 0 76 478 1997 2607
Size: 76 402 1519 610
Fragments arranged by size:
1519 610 402 76
BanI G'GyrC_C
Cuts at: 0 510 765 778 2348 2607
Size: 510 255 13 1570 259
Fragments arranged by size:
1570 510 259 255 13
BanII G_rGCy'C
Cuts at: 0 584 827 1664 1820 2607
Size: 584 243 837 156 787
Fragments arranged by size:
837 787 584 243 156
BbeI G_GCGC'C
Cuts at: 0 769 2607
Size: 769 1838
BbsI GAAGACnn'nnnn_
Cuts at: 0 414 2189 2460 2607
Size: 414 1775 271 147
Fragments arranged by size:
1775 414 271 147
BbvCI CC'TCA_GC
Cuts at: 0 2584 2607
Size: 2584 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2475 2509 2607
Size: 2475 34 98
Fragments arranged by size:
2475 98 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 467 2607
Size: 467 2140
BglI GCCn_nnn'nGGC
Cuts at: 0 368 1748 2329 2607
Size: 368 1380 581 278
Fragments arranged by size:
1380 581 368 278
Bme1580I G_kGCm'C
Cuts at: 0 1100 2607
Size: 1100 1507
BmgBI CAC'GTC
Cuts at: 0 2457 2607
Size: 2457 150
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 655 2159 2607
Size: 655 1504 448
Fragments arranged by size:
1504 655 448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 764 1228 1336 2607
Size: 596 168 464 108 1271
Fragments arranged by size:
1271 596 464 168 108
Bpu10I CC'TnA_GC
Cuts at: 0 1109 2584 2607
Size: 1109 1475 23
Fragments arranged by size:
1475 1109 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 2607
Size: 307 2300
BsaI GGTCTCn'nnnn_
Cuts at: 0 23 58 190 289 2607
Size: 23 35 132 99 2318
Fragments arranged by size:
2318 132 99 35 23
BsaAI yAC'GTr
Cuts at: 0 830 1737 2607
Size: 830 907 870
Fragments arranged by size:
907 870 830
BsaBI GATnn'nnATC
Cuts at: 0 158 2607
Size: 158 2449
BsaHI Gr'CG_yC
Cuts at: 0 766 2607
Size: 766 1841
BsaWI w'CCGG_w
Cuts at: 0 530 2607
Size: 530 2077
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 790 820 2188 2218 2516 2546 2607
Size: 790 30 1368 30 298 30 61
Fragments arranged by size:
1368 790 298 61 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 124 127 367 811 2104 2212 2282
Size: 94 30 3 240 444 1293 108 70
Cuts at: 2282 2607
Size: 325
Fragments arranged by size:
1293 444 325 240 108 94 70 30
3
BseYI C'CCAG_C
Cuts at: 0 905 2607
Size: 905 1702
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 252 378 1595 2607
Size: 30 222 126 1217 1012
Fragments arranged by size:
1217 1012 222 126 30
BsiEI CG_ry'CG
Cuts at: 0 2242 2607
Size: 2242 365
BsiHKAI G_wGCw'C
Cuts at: 0 842 958 1100 2078 2607
Size: 842 116 142 978 529
Fragments arranged by size:
978 842 529 142 116
BsmI GAATG_Cn'
Cuts at: 0 1198 2607
Size: 1198 1409
BsmBI CGTCTCn'nnnn_
Cuts at: 0 532 2445 2463 2607
Size: 532 1913 18 144
Fragments arranged by size:
1913 532 144 18
Bsp1286I G_dGCh'C
Cuts at: 0 584 827 842 958 1100 1664 1820 2078
Size: 584 243 15 116 142 564 156 258
Cuts at: 2078 2607
Size: 529
Fragments arranged by size:
584 564 529 258 243 156 142 116
15
BspEI T'CCGG_A
Cuts at: 0 530 2607
Size: 530 2077
BspHI T'CATG_A
Cuts at: 0 1937 2607
Size: 1937 670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 454 2510 2607
Size: 26 428 2056 97
Fragments arranged by size:
2056 428 97 26
BsrBI CCG'CTC
Cuts at: 0 736 2202 2607
Size: 736 1466 405
Fragments arranged by size:
1466 736 405
BsrFI r'CCGG_y
Cuts at: 0 453 2607
Size: 453 2154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 41 2124 2607
Size: 41 2083 483
Fragments arranged by size:
2083 483 41
BstEII G'GTnAC_C
Cuts at: 0 1149 2607
Size: 1149 1458
BstXI CCAn_nnnn'nTGG
Cuts at: 0 925 1161 1369 2607
Size: 925 236 208 1238
Fragments arranged by size:
1238 925 236 208
BstYI r'GATC_y
Cuts at: 0 671 2607
Size: 671 1936
BtgI C'CryG_G
Cuts at: 0 200 1157 1880 1917 2192 2223 2344 2507
Size: 200 957 723 37 275 31 121 163
Cuts at: 2507 2607
Size: 100
Fragments arranged by size:
957 723 275 200 163 121 100 37
31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2064 2607
Size: 2064 543
EaeI y'GGCC_r
Cuts at: 0 1313 1688 2330 2607
Size: 1313 375 642 277
Fragments arranged by size:
1313 642 375 277
EarI CTCTTCn'nnn_
Cuts at: 0 316 589 1215 1327 1363 2607
Size: 316 273 626 112 36 1244
Fragments arranged by size:
1244 626 316 273 112 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2197 2607
Size: 2197 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 764 1228 1336 2607
Size: 596 168 464 108 1271
Fragments arranged by size:
1271 596 464 168 108
EcoO109I rG'GnC_Cy
Cuts at: 0 549 601 1032 1556 2109 2607
Size: 549 52 431 524 553 498
Fragments arranged by size:
553 549 524 498 431 52
EcoRI G'AATT_C
Cuts at: 0 1263 2607
Size: 1263 1344
EcoRV GAT'ATC
Cuts at: 0 1731 2607
Size: 1731 876
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 2607
Size: 735 1872
FspI TGC'GCA
Cuts at: 0 1876 2607
Size: 1876 731
HaeII r_GCGC'y
Cuts at: 0 769 2300 2607
Size: 769 1531 307
Fragments arranged by size:
1531 769 307
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 790 822 2607
Size: 709 32 49 32 1785
Fragments arranged by size:
1785 709 49 32 32
KasI G'GCGC_C
Cuts at: 0 765 2607
Size: 765 1842
KpnI G_GTAC'C
Cuts at: 0 782 2352 2607
Size: 782 1570 255
Fragments arranged by size:
1570 782 255
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1411 2607
Size: 1411 1196
MscI TGG'CCA
Cuts at: 0 1315 1690 2607
Size: 1315 375 917
Fragments arranged by size:
1315 917 375
MslI CAynn'nnrTG
Cuts at: 0 1159 1367 1681 2398 2607
Size: 1159 208 314 717 209
Fragments arranged by size:
1159 717 314 209 208
MspA1I CmG'CkG
Cuts at: 0 1579 1919 2346 2607
Size: 1579 340 427 261
Fragments arranged by size:
1579 427 340 261
NarI GG'CG_CC
Cuts at: 0 766 2607
Size: 766 1841
NcoI C'CATG_G
Cuts at: 0 200 2192 2607
Size: 200 1992 415
Fragments arranged by size:
1992 415 200
NspI r_CATG'y
Cuts at: 0 1430 2067 2607
Size: 1430 637 540
Fragments arranged by size:
1430 637 540
PciI A'CATG_T
Cuts at: 0 2063 2607
Size: 2063 544
PflMI CCAn_nnn'nTGG
Cuts at: 0 1860 2367 2607
Size: 1860 507 240
Fragments arranged by size:
1860 507 240
PfoI T'CCnGG_A
Cuts at: 0 469 2607
Size: 469 2138
PmlI CAC'GTG
Cuts at: 0 830 1737 2607
Size: 830 907 870
Fragments arranged by size:
907 870 830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1918 1950 2188 2220 2607
Size: 1918 32 238 32 387
Fragments arranged by size:
1918 387 238 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 549 1556 2109 2607
Size: 549 1007 553 498
Fragments arranged by size:
1007 553 549 498
PshAI GACnn'nnGTC
Cuts at: 0 1203 1831 2607
Size: 1203 628 776
Fragments arranged by size:
1203 776 628
PstI C_TGCA'G
Cuts at: 0 37 756 2120 2576 2607
Size: 37 719 1364 456 31
Fragments arranged by size:
1364 719 456 37 31
SacII CC_GC'GG
Cuts at: 0 1920 2347 2607
Size: 1920 427 260
Fragments arranged by size:
1920 427 260
SapI GCTCTTCn'nnn_
Cuts at: 0 589 1363 2607
Size: 589 774 1244
Fragments arranged by size:
1244 774 589
SfcI C'TryA_G
Cuts at: 0 33 752 2116 2572 2607
Size: 33 719 1364 456 35
Fragments arranged by size:
1364 719 456 35 33
SfoI GGC'GCC
Cuts at: 0 767 2607
Size: 767 1840
SmaI CCC'GGG
Cuts at: 0 1999 2607
Size: 1999 608
SmlI C'TyrA_G
Cuts at: 0 322 2607
Size: 322 2285
StyI C'CwwG_G
Cuts at: 0 93 130 200 552 819 2192 2607
Size: 93 37 70 352 267 1373 415
Fragments arranged by size:
1373 415 352 267 93 70 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 331 2228 2467 2607
Size: 331 1897 239 140
Fragments arranged by size:
1897 331 239 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2052 2084 2516 2548 2607
Size: 2052 32 432 32 59
Fragments arranged by size:
2052 432 59 32 32
XbaI T'CTAG_A
Cuts at: 0 792 2607
Size: 792 1815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1809 2183 2607
Size: 1809 374 424
Fragments arranged by size:
1809 424 374
XmaI C'CCGG_G
Cuts at: 0 1997 2607
Size: 1997 610
XmnI GAAnn'nnTTC
Cuts at: 0 1933 2607
Size: 1933 674
Enzymes that do cut:
AarI AccI Acc65I AflIII AleI AlfI AloI AlwNI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI
BtgI BtgZI EaeI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NspI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PstI SacII SapI
SfcI SfoI SmaI SmlI StyI TaqII TstI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AhdI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BclI BfrBI
BglII BlpI BmrI BmtI BsiWI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI FseI FspAI HincII
HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XhoI
ZraI