Restriction map for
pFN21ASDA0036
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# NO CUT ENZYMES ( 133 enzymes) #
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Acc65I AccII AccIII AciI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HapII HgaI HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SalI SbfI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 773 GACGTC GACGT^C
AccI 776 GTCTAC GT^[AC][GT]AC
AclI 1549 AACGTT AA^CGTT
AcyI 773 GACGTC G[GA]^CG[CT]C
AflIII 370 ACATGT A^C[GA][CT]GT
Aor51HI 1278 AGCGCT AGC^GCT
ApaI 1490 GGGCCC GGGCC^C
AvaI 1088 CCCGAG C^[CT]CG[GA]G
BanI 1569 GGCACC G^G[CT][GA]CC
BbsI 1400 GAAGAC GAAGAC(2/6)
BclI 498 TGATCA T^GATCA
BglI 1645 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1400 GAAGAC GAAGAC(2/6)
BsaHI 773 GACGTC G[GA]^CG[CT]C
BseRI 975 GAGGAG GAGGAG(10/8)
BsiYI 1327 CCTTGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1327 CCTTGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 1088 CCCGAG C^[CT]CG[GA]G
BspHI 551 TCATGA T^CATGA
BspLU11I 370 ACATGT A^CATGT
BspMI 754 ACCTGC ACCTGC(4/8)
BsrDI 265 CATTGC GCAATG(2/0)
BstXI 1625 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1279 GCGC GCG^C
DrdI 767 GACTCAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1278 AGCGCT AGC^GCT
EcoNI 1327 CCTTGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 805 GAATTC G^AATTC
FbaI 498 TGATCA T^GATCA
HaeII 1278 AGCGCT [GA]GCGC^[CT]
HhaI 1279 GCGC GCG^C
Hin1I 773 GACGTC G[GA]^CG[CT]C
HinP1I 1279 GCGC G^CGC
MfeI 1051 CAATTG C^AATTG
MunI 1051 CAATTG C^AATTG
PpuMI 1037 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1549 AACGTT AA^CGTT
RcaI 551 TCATGA T^CATGA
ScaI 682 AGTACT AGT^ACT
SfiI 1644 GGCCAAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1457 CTCAAG C^T[CT][GA]AG
SphI 892 GCATGC GCATG^C
SspI 179 AATATT AAT^ATT
StuI 1627 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspHI 1441 GAGCTC G[AT]GC[AT]^C
1606 GAGCTC
AspI 526 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1390 GACTATGTC
AvaII 192 GGTCC G^G[AT]CC
1038 GGACC
BpmI 1009 CTGGAG CTGGAG(16/14)
1715 CTGGAG
BsiHKAI 1441 GAGCTC G[AT]GC[AT]^C
1606 GAGCTC
BsmFI 799 GGGAC GGGAC(10/14)
73 GTCCC
BsrI 796 ACTGG ACTGG(1/-1)
670 CCAGT
HphI 589 GGTGA GGTGA(8/7)
1221 TCACC
MaeII 774 ACGT A^CGT
1550 ACGT
MaeIII 433 GTGAC ^GT[ACGT]AC
1261 GTCAC
NspI 370 ACATGT [GA]CATG^[CT]
892 GCATGC
PflFI 526 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1390 GACTATGTC
PleI 767 GACTC GAGTC(4/5)
1337 GACTC
PstI 332 CTGCAG CTGCA^G
545 CTGCAG
SacI 1441 GAGCTC GAGCT^C
1606 GAGCTC
TaqI 213 TCGA T^CGA
1112 TCGA
Tsp45I 433 GTGAC ^GT[GC]AC
1261 GTCAC
Tth111I 526 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1390 GACTATGTC
TthHB8I 213 TCGA T^CGA
1112 TCGA
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BanII BbvI BfaI BmyI
BsaJI BsmAI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HindIII
HinfI ItaI Ksp632I MaeI MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII XmnI