(Circular) (Six-base) MAPSORT of: pFN21ASDA0036.seq Check: 7185 from: 1 to: 6523 pFN21ASDA0036 6523 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2800 4333 278 Size: 53 83 186 936 1264 1533 2468 Fragments arranged by size: 2468 1533 1264 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2800 3866 1937 Size: 863 1066 4594 Fragments arranged by size: 4594 1066 863 Acc65I G'GTAC_C Cuts at: 3844 3844 Size: 6523 AclI AA'CG_TT Cuts at: 1563 3573 4650 5023 1563 Size: 2010 1077 373 3063 Fragments arranged by size: 3063 2010 1077 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 2439 3416 3623 3713 4586 5970 6238 Size: 456 351 977 207 90 873 1384 268 Cuts at: 6238 1632 Size: 1917 Fragments arranged by size: 1917 1384 977 873 456 351 268 207 90 AfeI AGC'GCT Cuts at: 3303 3303 Size: 6523 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6326 AflIII A'CryG_T Cuts at: 1203 2393 5422 6399 1203 Size: 1190 3029 977 1327 Fragments arranged by size: 3029 1327 1190 977 AgeI A'CCGG_T Cuts at: 5354 5354 Size: 6523 AhdI GACnn_n'nnGTC Cuts at: 5252 5252 Size: 6523 AleI CACnn'nnGTG Cuts at: 1166 3712 1166 Size: 2546 3977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6523 AlwNI CAG_nnn'CTG Cuts at: 4266 5838 4266 Size: 1572 4951 ApaI G_GGCC'C Cuts at: 3517 3517 Size: 6523 ApaLI G'TGCA_C Cuts at: 4580 5736 4580 Size: 1156 5367 ApoI r'AATT_y Cuts at: 1493 2327 2362 2751 2828 3238 3308 3787 3832 Size: 834 35 389 77 410 70 479 45 Cuts at: 3832 4058 4210 6319 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 479 410 389 226 152 77 70 45 35 AseI AT'TA_AT Cuts at: 160 1032 5077 160 Size: 872 4045 1606 Fragments arranged by size: 4045 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 AvaI C'yCGr_G Cuts at: 1870 1943 3111 3848 1870 Size: 73 1168 737 4545 Fragments arranged by size: 4545 1168 737 73 BamHI G'GATC_C Cuts at: 3853 4220 3853 Size: 367 6156 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3592 3844 5299 618 Size: 325 217 627 1805 252 1455 1842 Fragments arranged by size: 1842 1805 1455 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 3437 3468 3517 3532 3633 3842 1391 Size: 2046 31 49 15 101 209 4072 Fragments arranged by size: 4072 2046 209 101 49 31 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3430 928 Size: 971 1531 4021 Fragments arranged by size: 4021 1531 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4714 4748 1903 Size: 34 2777 34 3678 Fragments arranged by size: 3678 2777 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4415 5625 4415 Size: 1210 5313 BclI T'GATC_A Cuts at: 1548 2521 1548 Size: 973 5550 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3674 5134 136 Size: 107 122 71 631 605 2002 1460 1525 Fragments arranged by size: 2002 1525 1460 631 605 122 107 71 BglII A'GATC_T Cuts at: 6518 6518 Size: 6523 BlpI GC'TnA_GC Cuts at: 3940 3940 Size: 6523 Bme1580I G_kGCm'C Cuts at: 1790 3517 4584 5740 1790 Size: 1727 1067 1156 2573 Fragments arranged by size: 2573 1727 1156 1067 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6523 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2829 4254 5212 Size: 416 53 83 399 24 1412 1425 958 Cuts at: 5212 442 Size: 1753 Fragments arranged by size: 1753 1425 1412 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6523 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3276 3276 Size: 6523 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3053 3759 5183 1239 Size: 1814 706 1424 2579 Fragments arranged by size: 2579 1814 1424 706 Bpu10I CC'TnA_GC Cuts at: 2085 2412 3627 2085 Size: 327 1215 4981 Fragments arranged by size: 4981 1215 327 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3465 4648 5513 5811 3465 Size: 1183 865 298 4177 Fragments arranged by size: 4177 1183 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5186 882 Size: 617 3687 2219 Fragments arranged by size: 3687 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3820 4314 493 Size: 711 2616 494 2702 Fragments arranged by size: 2702 2616 711 494 BsaBI GATnn'nnATC Cuts at: 6517 6517 Size: 6523 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2797 4330 4712 5347 Size: 53 83 186 936 1264 1533 382 635 Cuts at: 5347 275 Size: 1451 Fragments arranged by size: 1533 1451 1264 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4956 5354 5628 5775 6186 4956 Size: 398 274 147 411 5293 Fragments arranged by size: 5293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2475 2505 1178 Size: 30 165 30 1072 30 5196 Fragments arranged by size: 5196 1072 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3013 1186 Size: 1827 4696 BseYI C'CCAG_C Cuts at: 2265 3531 5726 6222 2265 Size: 1266 2195 496 2566 Fragments arranged by size: 2566 2195 1266 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5950 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3973 4734 5762 1540 Size: 275 186 1972 761 1028 2301 Fragments arranged by size: 2301 1972 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3468 3633 4584 4669 5740 3468 Size: 165 951 85 1071 4251 Fragments arranged by size: 4251 1071 951 165 85 BsmI GAATG_Cn' Cuts at: 4034 4127 4034 Size: 93 6430 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3437 3468 3517 3532 3633 3842 4584 Size: 399 1647 31 49 15 101 209 742 Cuts at: 4584 4669 5740 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1647 1071 742 399 209 101 85 49 31 15 BspEI T'CCGG_A Cuts at: 6186 6186 Size: 6523 BspHI T'CATG_A Cuts at: 2574 4412 6142 2574 Size: 1838 1730 2955 Fragments arranged by size: 2955 1838 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2786 3878 844 Size: 1942 1092 3489 Fragments arranged by size: 3489 1942 1092 BsrBI CCG'CTC Cuts at: 1394 1823 4410 1394 Size: 429 2587 3507 Fragments arranged by size: 3507 2587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2287 5018 5192 65 Size: 1183 1039 2731 174 1396 Fragments arranged by size: 2731 1396 1183 1039 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5167 5354 1700 Size: 110 3357 187 2869 Fragments arranged by size: 3357 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6523 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6523 BssSI C'ACGA_G Cuts at: 4583 5595 4583 Size: 1012 5511 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6523 BstBI TT'CG_AA Cuts at: 2007 5327 2007 Size: 3320 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6523 BstXI CCAn_nnnn'nTGG Cuts at: 3655 3655 Size: 6523 BstYI r'GATC_y Cuts at: 1972 2049 2564 3008 3644 3853 4220 4605 4622 Size: 77 515 444 636 209 367 385 17 Cuts at: 4622 6074 6160 6172 6518 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 636 515 444 385 367 346 209 86 77 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5971 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5784 Fragments arranged by size: 5784 683 56 BtsI GCAGTG_nn' Cuts at: 4039 4833 4853 4039 Size: 794 20 5709 Fragments arranged by size: 5709 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6523 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6488 DraI TTT'AAA Cuts at: 3827 3827 Size: 6523 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2796 5530 809 Size: 507 1480 2734 1802 Fragments arranged by size: 2734 1802 1480 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3970 4859 6488 Size: 54 1425 50 275 18 2140 889 1629 Cuts at: 6488 8 Size: 43 Fragments arranged by size: 2140 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3970 1537 Size: 275 2158 4090 Fragments arranged by size: 4090 2158 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2474 2886 3051 3177 3516 3638 4453 1353 Size: 1121 412 165 126 339 122 815 3423 Fragments arranged by size: 3423 1121 815 412 339 165 126 122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5110 5484 5630 387 Size: 1064 138 3521 374 146 1280 Fragments arranged by size: 3521 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3466 3631 3466 Size: 165 6358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2439 3053 3416 3623 3713 3759 Size: 393 456 351 614 363 207 90 46 Cuts at: 3759 4586 5183 5970 6238 1239 Size: 827 597 787 268 1524 Fragments arranged by size: 1524 827 787 614 597 456 393 363 351 268 207 90 46 EcoNI CCTnn'n_nnAGG Cuts at: 3354 3354 Size: 6523 EcoO109I rG'GnC_Cy Cuts at: 3061 3513 3514 3061 Size: 452 1 6070 Fragments arranged by size: 6070 452 1 EcoRI G'AATT_C Cuts at: 2828 3832 4210 2828 Size: 1004 378 5141 Fragments arranged by size: 5141 1004 378 FspI TGC'GCA Cuts at: 5029 5029 Size: 6523 HaeII r_GCGC'y Cuts at: 1678 3305 5670 1678 Size: 1627 2365 2531 Fragments arranged by size: 2531 2365 1627 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2449 2481 2556 2588 5164 Size: 32 135 32 877 32 75 32 2576 Cuts at: 5164 5196 5238 5270 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2576 877 135 75 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3867 4113 669 Size: 988 281 1929 246 3079 Fragments arranged by size: 3079 1929 988 281 246 HindIII A'AGCT_T Cuts at: 748 2672 2969 3722 748 Size: 1924 297 753 3549 Fragments arranged by size: 3549 1924 753 297 HpaI GTT'AAC Cuts at: 4113 4113 Size: 6523 KpnI G_GTAC'C Cuts at: 3848 3848 Size: 6523 MfeI C'AATT_G Cuts at: 3074 4122 3074 Size: 1048 5475 MluI A'CGCG_T Cuts at: 6399 6399 Size: 6523 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2231 5637 5821 1305 Size: 926 3406 184 2007 Fragments arranged by size: 3406 2007 926 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4701 Fragments arranged by size: 4701 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2292 2579 3712 4481 4840 4999 518 Size: 648 1126 287 1133 769 359 159 2042 Fragments arranged by size: 2042 1133 1126 769 648 359 287 159 MspA1I CmG'CkG Cuts at: 1606 2354 3124 3281 3939 4616 5764 6009 1606 Size: 748 770 157 658 677 1148 245 2120 Fragments arranged by size: 2120 1148 770 748 677 658 245 157 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5971 NdeI CA'TA_TG Cuts at: 387 387 Size: 6523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6523 NotI GC'GGCC_GC Cuts at: 3970 3970 Size: 6523 NspI r_CATG'y Cuts at: 1132 1229 2397 2919 3881 5426 1132 Size: 97 1168 522 962 1545 2229 Fragments arranged by size: 2229 1545 1168 962 522 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6523 PciI A'CATG_T Cuts at: 2393 5422 2393 Size: 3029 3494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6523 PmeI GTTT'AAAC Cuts at: 3827 3827 Size: 6523 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3615 3647 4592 4624 6105 6137 3615 Size: 32 945 32 1481 32 4001 Fragments arranged by size: 4001 1481 945 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3061 3061 Size: 6523 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6523 PsiI TTA'TAA Cuts at: 2699 4093 2699 Size: 1394 5129 PspOMI G'GGCC_C Cuts at: 3513 3513 Size: 6523 PstI C_TGCA'G Cuts at: 830 2359 2572 3875 830 Size: 1529 213 1303 3478 Fragments arranged by size: 3478 1529 1303 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6523 PvuII CAG'CTG Cuts at: 2354 3124 3281 2354 Size: 770 157 5596 Fragments arranged by size: 5596 770 157 SacI G_AGCT'C Cuts at: 3468 3633 3468 Size: 165 6358 SalI G'TCGA_C Cuts at: 1936 3865 1936 Size: 1929 4594 SapI GCTCTTCn'nnn_ Cuts at: 2886 3516 3638 2886 Size: 630 122 5771 Fragments arranged by size: 5771 630 122 SbfI CC_TGCA'GG Cuts at: 3875 3875 Size: 6523 ScaI AGT'ACT Cuts at: 2707 4771 6483 2707 Size: 2064 1712 2747 Fragments arranged by size: 2747 2064 1712 SfcI C'TryA_G Cuts at: 826 1045 2058 2355 2568 2585 3871 5006 5687 Size: 219 1013 297 213 17 1286 1135 681 Cuts at: 5687 5878 826 Size: 191 1471 Fragments arranged by size: 1471 1286 1135 1013 681 297 219 213 191 17 SfiI GGCCn_nnn'nGGCC Cuts at: 3674 3674 Size: 6523 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6523 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6523 SmaI CCC'GGG Cuts at: 1872 3850 1872 Size: 1978 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3480 4627 5528 5790 820 Size: 197 926 1537 1147 901 262 1553 Fragments arranged by size: 1553 1537 1147 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3820 4314 493 Size: 3327 494 2702 Fragments arranged by size: 3327 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6523 SphI G_CATG'C Cuts at: 1132 2919 3881 1132 Size: 1787 962 3774 Fragments arranged by size: 3774 1787 962 SspI AAT'ATT Cuts at: 5 52 2204 4447 5 Size: 47 2152 2243 2081 Fragments arranged by size: 2243 2152 2081 47 StuI AGG'CCT Cuts at: 1517 3652 1517 Size: 2135 4388 StyI C'CwwG_G Cuts at: 513 1065 2045 3350 3663 3962 513 Size: 552 980 1305 313 299 3074 Fragments arranged by size: 3074 1305 980 552 313 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4550 4567 4720 4905 5350 325 Size: 1255 2970 17 153 185 445 1498 Fragments arranged by size: 2970 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2509 2705 4769 Size: 274 80 33 51 1443 532 196 2064 Cuts at: 4769 6481 96 Size: 1712 138 Fragments arranged by size: 2064 1712 1443 532 274 196 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3186 3218 6483 6515 3186 Size: 32 3265 32 3194 Fragments arranged by size: 3265 3194 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2552 3416 1316 Size: 1236 864 4423 Fragments arranged by size: 4423 1236 864 XbaI T'CTAG_A Cuts at: 3859 3859 Size: 6523 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6523 XmaI C'CCGG_G Cuts at: 1870 3848 1870 Size: 1978 4545 XmnI GAAnn'nnTTC Cuts at: 2343 2364 2499 3141 4652 2343 Size: 21 135 642 1511 4214 Fragments arranged by size: 4214 1511 642 135 21 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2798 4331 276 Size: 53 83 186 936 1264 1533 2468 Fragments arranged by size: 2468 1533 1264 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI