(Circular) (Six-base) MAPSORT of: pFN21ASDA0035.seq Check: 1337 from: 1 to: 6856 pFN21ASDA0035 6856 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3253 3253 Size: 6856 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4666 278 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4199 1937 Size: 2262 4594 Acc65I G'GTAC_C Cuts at: 3197 4177 3197 Size: 980 5876 AclI AA'CG_TT Cuts at: 1563 4983 5356 1563 Size: 3420 373 3063 Fragments arranged by size: 3420 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3072 3123 3851 4919 6303 6571 1632 Size: 1440 51 728 1068 1384 268 1917 Fragments arranged by size: 1917 1440 1384 1068 728 268 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6659 AflIII A'CryG_T Cuts at: 1203 5755 6732 1203 Size: 4552 977 1327 Fragments arranged by size: 4552 1327 977 AgeI A'CCGG_T Cuts at: 5687 5687 Size: 6856 AhdI GACnn_n'nnGTC Cuts at: 5585 5585 Size: 6856 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6856 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6856 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2959 2991 2959 Size: 32 6824 AlwNI CAG_nnn'CTG Cuts at: 2777 3155 3575 4599 6171 2777 Size: 378 420 1024 1572 3462 Fragments arranged by size: 3462 1572 1024 420 378 ApaLI G'TGCA_C Cuts at: 4913 6069 4913 Size: 1156 5700 ApoI r'AATT_y Cuts at: 1493 3208 4165 4391 4543 6652 1493 Size: 1715 957 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 957 226 152 AseI AT'TA_AT Cuts at: 160 1032 5410 160 Size: 872 4378 1606 Fragments arranged by size: 4378 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 AvaI C'yCGr_G Cuts at: 1870 1943 2631 4181 1870 Size: 73 688 1550 4545 Fragments arranged by size: 4545 1550 688 73 BamHI G'GATC_C Cuts at: 4186 4553 4186 Size: 367 6489 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3197 3695 4177 5632 618 Size: 325 217 627 1410 498 482 1455 1842 Fragments arranged by size: 1842 1455 1410 627 498 482 325 217 BanII G_rGCy'C Cuts at: 1391 2522 3137 3665 4175 1391 Size: 1131 615 528 510 4072 Fragments arranged by size: 4072 1131 615 528 510 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3027 3368 3509 928 Size: 971 1128 341 141 4275 Fragments arranged by size: 4275 1128 971 341 141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5047 5081 1903 Size: 34 3110 34 3678 Fragments arranged by size: 3678 3110 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2076 4748 5958 2076 Size: 2672 1210 2974 Fragments arranged by size: 2974 2672 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6856 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3434 3686 3833 Size: 107 122 71 631 605 1762 252 147 Cuts at: 3833 5467 136 Size: 1634 1525 Fragments arranged by size: 1762 1634 1525 631 605 252 147 122 107 71 BglII A'GATC_T Cuts at: 6851 6851 Size: 6856 BlpI GC'TnA_GC Cuts at: 4273 4273 Size: 6856 Bme1580I G_kGCm'C Cuts at: 1790 4917 6073 1790 Size: 3127 1156 2573 Fragments arranged by size: 3127 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6856 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2804 4021 4587 Size: 416 53 83 399 24 1387 1217 566 Cuts at: 4587 5545 442 Size: 958 1753 Fragments arranged by size: 1753 1387 1217 958 566 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6856 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2878 3361 2878 Size: 483 6373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2307 2848 3096 3310 3331 3463 3610 5516 Size: 1068 541 248 214 21 132 147 1906 Cuts at: 5516 1239 Size: 2579 Fragments arranged by size: 2579 1906 1068 541 248 214 147 132 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2180 2524 3635 4981 5846 6144 2180 Size: 344 1111 1346 865 298 2892 Fragments arranged by size: 2892 1346 1111 865 344 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5519 882 Size: 617 4020 2219 Fragments arranged by size: 4020 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4153 4647 493 Size: 711 2949 494 2702 Fragments arranged by size: 2949 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6850 6850 Size: 6856 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4663 5045 5680 275 Size: 53 83 186 936 3130 382 635 1451 Fragments arranged by size: 3130 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5289 5687 5961 6108 6519 5289 Size: 398 274 147 411 5626 Fragments arranged by size: 5626 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2528 2558 2959 2989 3308 Size: 30 165 30 1125 30 401 30 319 Cuts at: 3308 3338 1178 Size: 30 4696 Fragments arranged by size: 4696 1125 401 319 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 2315 2318 2450 2490 2729 2732 2894 Size: 1126 3 3 132 40 239 3 162 Cuts at: 2894 3368 3371 3429 3503 3506 3758 3761 3834 Size: 474 3 58 74 3 252 3 73 Cuts at: 3834 3956 1186 Size: 122 4086 Fragments arranged by size: 4086 1126 474 252 239 162 132 122 74 73 58 40 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2054 2970 6059 6555 2054 Size: 916 3089 496 2355 Fragments arranged by size: 3089 2355 916 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3573 3687 4020 1163 Size: 573 1837 114 333 3999 Fragments arranged by size: 3999 1837 573 333 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 4306 5067 6095 1540 Size: 275 186 30 2275 761 1028 2301 Fragments arranged by size: 2301 2275 1028 761 275 186 30 BsiHKAI G_wGCw'C Cuts at: 2079 2522 3137 4917 5002 6073 2079 Size: 443 615 1780 85 1071 2862 Fragments arranged by size: 2862 1780 1071 615 443 85 BsmI GAATG_Cn' Cuts at: 2037 4367 4460 2037 Size: 2330 93 4433 Fragments arranged by size: 4433 2330 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2522 3137 3665 4175 4917 5002 Size: 399 289 443 615 528 510 742 85 Cuts at: 5002 6073 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 615 528 510 443 399 289 85 BspEI T'CCGG_A Cuts at: 6519 6519 Size: 6856 BspHI T'CATG_A Cuts at: 4745 6475 4745 Size: 1730 5126 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2353 3253 4211 844 Size: 1509 900 958 3489 Fragments arranged by size: 3489 1509 958 900 BsrBI CCG'CTC Cuts at: 1394 1823 4743 1394 Size: 429 2920 3507 Fragments arranged by size: 3507 2920 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5351 5525 65 Size: 1183 4103 174 1396 Fragments arranged by size: 4103 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 5500 5687 1700 Size: 110 223 3467 187 2869 Fragments arranged by size: 3467 2869 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6856 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6856 BssSI C'ACGA_G Cuts at: 2072 3963 4916 5928 2072 Size: 1891 953 1012 3000 Fragments arranged by size: 3000 1891 1012 953 BstAPI GCAn_nnn'nTGC Cuts at: 794 3551 3710 794 Size: 2757 159 3940 Fragments arranged by size: 3940 2757 159 BstBI TT'CG_AA Cuts at: 2007 5660 2007 Size: 3653 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6856 BstYI r'GATC_y Cuts at: 1972 4186 4553 4938 4955 6407 6493 6505 6851 Size: 2214 367 385 17 1452 86 12 346 Cuts at: 6851 1972 Size: 1977 Fragments arranged by size: 2214 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3441 3846 3441 Size: 405 6451 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5347 Fragments arranged by size: 5347 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6117 Fragments arranged by size: 6117 683 56 BtsI GCAGTG_nn' Cuts at: 2413 2707 2857 3022 3034 3255 3490 3780 4372 Size: 294 150 165 12 221 235 290 592 Cuts at: 4372 5166 5186 2413 Size: 794 20 4083 Fragments arranged by size: 4083 794 592 294 290 235 221 165 150 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6856 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6821 DraI TTT'AAA Cuts at: 4160 4160 Size: 6856 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3511 5863 809 Size: 507 2195 2352 1802 Fragments arranged by size: 2352 2195 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2112 2799 Size: 54 1425 50 275 18 198 84 687 Cuts at: 2799 3289 3378 4303 5192 6821 8 Size: 490 89 925 889 1629 43 Fragments arranged by size: 1629 1425 925 889 687 490 275 198 89 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2028 4303 1537 Size: 275 216 2275 4090 Fragments arranged by size: 4090 2275 275 216 EarI CTCTTCn'nnn_ Cuts at: 1353 2170 2406 2834 2871 3051 3142 3330 3492 Size: 817 236 428 37 180 91 188 162 Cuts at: 3492 3630 3636 4786 1353 Size: 138 6 1150 3423 Fragments arranged by size: 3423 1150 817 428 236 188 180 162 138 91 37 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5443 5817 5963 387 Size: 1064 138 3854 374 146 1280 Fragments arranged by size: 3854 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2520 3135 2520 Size: 615 6241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2848 3072 3096 3123 3310 3331 Size: 393 675 541 224 24 27 187 21 Cuts at: 3331 3463 3610 3851 4919 5516 6303 6571 1239 Size: 132 147 241 1068 597 787 268 1524 Fragments arranged by size: 1524 1068 787 675 597 541 393 268 241 224 187 147 132 27 24 21 EcoO109I rG'GnC_Cy Cuts at: 2209 2318 2806 2912 2209 Size: 109 488 106 6153 Fragments arranged by size: 6153 488 109 106 EcoRI G'AATT_C Cuts at: 3208 4165 4543 3208 Size: 957 378 5521 Fragments arranged by size: 5521 957 378 FspI TGC'GCA Cuts at: 5362 5362 Size: 6856 HaeII r_GCGC'y Cuts at: 1678 6003 1678 Size: 4325 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2528 2554 2560 3308 Size: 32 135 32 950 6 26 6 748 Cuts at: 3308 3340 3446 3478 3491 3523 4095 4127 5497 Size: 32 106 32 13 32 572 32 1370 Cuts at: 5497 5529 5571 5603 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1370 950 748 572 135 106 42 32 32 32 32 32 32 32 32 26 13 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 4200 4446 669 Size: 988 281 2262 246 3079 Fragments arranged by size: 3079 2262 988 281 246 HindIII A'AGCT_T Cuts at: 748 3296 3863 748 Size: 2548 567 3741 Fragments arranged by size: 3741 2548 567 HpaI GTT'AAC Cuts at: 4446 4446 Size: 6856 KpnI G_GTAC'C Cuts at: 3201 4181 3201 Size: 980 5876 MfeI C'AATT_G Cuts at: 4455 4455 Size: 6856 MluI A'CGCG_T Cuts at: 6732 6732 Size: 6856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5970 6154 1305 Size: 4665 184 2007 Fragments arranged by size: 4665 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2114 2801 3291 3380 10 Size: 54 1425 343 282 687 490 89 3486 Fragments arranged by size: 3486 1425 687 490 343 282 89 54 MslI CAynn'nnrTG Cuts at: 518 1166 4814 5173 5332 518 Size: 648 3648 359 159 2042 Fragments arranged by size: 3648 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3194 3227 3260 4272 4949 6097 6342 1606 Size: 1588 33 33 1012 677 1148 245 2120 Fragments arranged by size: 2120 1588 1148 1012 677 245 33 33 NaeI GCC'GGC Cuts at: 2035 2035 Size: 6856 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5347 Fragments arranged by size: 5347 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6856 NgoMIV G'CCGG_C Cuts at: 2033 2033 Size: 6856 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6856 NotI GC'GGCC_GC Cuts at: 2028 4303 2028 Size: 2275 4581 NspI r_CATG'y Cuts at: 1132 1229 4214 5759 1132 Size: 97 2985 1545 2229 Fragments arranged by size: 2985 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6856 PciI A'CATG_T Cuts at: 5755 5755 Size: 6856 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6856 PmeI GTTT'AAAC Cuts at: 4160 4160 Size: 6856 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2959 2991 4925 4957 6438 6470 2959 Size: 32 1934 32 1481 32 3345 Fragments arranged by size: 3345 1934 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 2912 2209 Size: 703 6153 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6856 PsiI TTA'TAA Cuts at: 4426 4426 Size: 6856 PspXI vC'TCGA_Gb Cuts at: 2631 2631 Size: 6856 PstI C_TGCA'G Cuts at: 830 2383 3250 3265 3520 4208 830 Size: 1553 867 15 255 688 3478 Fragments arranged by size: 3478 1553 867 688 255 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6856 PvuII CAG'CTG Cuts at: 3194 3227 3260 3194 Size: 33 33 6790 Fragments arranged by size: 6790 33 33 SacI G_AGCT'C Cuts at: 2522 3137 2522 Size: 615 6241 SalI G'TCGA_C Cuts at: 1936 4198 1936 Size: 2262 4594 SapI GCTCTTCn'nnn_ Cuts at: 3142 3142 Size: 6856 SbfI CC_TGCA'GG Cuts at: 4208 4208 Size: 6856 ScaI AGT'ACT Cuts at: 5104 6816 5104 Size: 1712 5144 SexAI A'CCwGG_T Cuts at: 2908 2908 Size: 6856 SfcI C'TryA_G Cuts at: 826 1045 2182 2379 2917 3246 3261 3516 4204 Size: 219 1137 197 538 329 15 255 688 Cuts at: 4204 5339 6020 6211 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1137 1135 688 681 538 329 255 219 197 191 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6856 SmaI CCC'GGG Cuts at: 1872 4183 1872 Size: 2311 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 2539 2631 3650 4960 5861 Size: 197 926 252 344 92 1019 1310 901 Cuts at: 5861 6123 820 Size: 262 1553 Fragments arranged by size: 1553 1310 1019 926 901 344 262 252 197 92 SnaBI TAC'GTA Cuts at: 493 4153 4647 493 Size: 3660 494 2702 Fragments arranged by size: 3660 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6856 SphI G_CATG'C Cuts at: 1132 4214 1132 Size: 3082 3774 SspI AAT'ATT Cuts at: 5 52 4780 5 Size: 47 4728 2081 Fragments arranged by size: 4728 2081 47 StuI AGG'CCT Cuts at: 1517 2267 1517 Size: 750 6106 StyI C'CwwG_G Cuts at: 513 1065 2022 2205 2484 2802 3393 3684 3828 Size: 552 957 183 279 318 591 291 144 Cuts at: 3828 4295 513 Size: 467 3074 Fragments arranged by size: 3074 957 591 552 467 318 291 279 183 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2348 4883 4900 5053 5238 5683 325 Size: 1255 768 2535 17 153 185 445 1498 Fragments arranged by size: 2535 1498 1255 768 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5102 6814 96 Size: 274 80 33 51 1443 3125 1712 138 Fragments arranged by size: 3125 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6816 6848 6816 Size: 32 6824 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6856 XbaI T'CTAG_A Cuts at: 4192 4192 Size: 6856 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2986 3277 2986 Size: 291 6565 XhoI C'TCGA_G Cuts at: 1943 2631 1943 Size: 688 6168 XmaI C'CCGG_G Cuts at: 1870 4181 1870 Size: 2311 4545 XmnI GAAnn'nnTTC Cuts at: 4985 4985 Size: 6856 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4664 276 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI