Restriction map for
pFN21ASDA0035
(Linear) (Six-base) MAPSORT of: KIAA0035.seq Check: 91 from: 1 to: 2127
KIAA0035.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1230 2127
Size: 1230 897
Acc65I G'GTAC_C
Cuts at: 0 1174 2127
Size: 1174 953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 1100 1828 2127
Size: 1049 51 728 299
Fragments arranged by size:
1049 728 299 51
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 936 968 2127
Size: 936 32 1159
Fragments arranged by size:
1159 936 32
AlwNI CAG_nnn'CTG
Cuts at: 0 754 1132 1552 2127
Size: 754 378 420 575
Fragments arranged by size:
754 575 420 378
ApoI r'AATT_y
Cuts at: 0 1185 2127
Size: 1185 942
AvaI C'yCGr_G
Cuts at: 0 608 2127
Size: 608 1519
BanI G'GyrC_C
Cuts at: 0 1174 1672 2127
Size: 1174 498 455
Fragments arranged by size:
1174 498 455
BanII G_rGCy'C
Cuts at: 0 499 1114 1642 2127
Size: 499 615 528 485
Fragments arranged by size:
615 528 499 485
BbsI GAAGACnn'nnnn_
Cuts at: 0 1004 1345 1486 2127
Size: 1004 341 141 641
Fragments arranged by size:
1004 641 341 141
BciVI GTATCCnnnnn_n'
Cuts at: 0 53 2127
Size: 53 2074
BglI GCCn_nnn'nGGC
Cuts at: 0 1411 1663 1810 2127
Size: 1411 252 147 317
Fragments arranged by size:
1411 317 252 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 781 1998 2127
Size: 781 1217 129
Fragments arranged by size:
1217 781 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 855 1338 2127
Size: 855 483 789
Fragments arranged by size:
855 789 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 825 1073 1287 1308 1440 1587 2127
Size: 284 541 248 214 21 132 147 540
Fragments arranged by size:
541 540 284 248 214 147 132 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 501 1612 2127
Size: 157 344 1111 515
Fragments arranged by size:
1111 515 344 157
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 936 966 1285 1315 2127
Size: 505 30 401 30 319 30 812
Fragments arranged by size:
812 505 401 319 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 292 295 427 467 706 709 871
Size: 289 3 3 132 40 239 3 162
Cuts at: 871 1345 1348 1406 1480 1483 1735 1738 1811
Size: 474 3 58 74 3 252 3 73
Cuts at: 1811 1933 2127
Size: 122 194
Fragments arranged by size:
474 289 252 239 194 162 132 122
74 73 58 40 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 31 947 2127
Size: 31 916 1180
Fragments arranged by size:
1180 916 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1550 1664 1997 2127
Size: 1550 114 333 130
Fragments arranged by size:
1550 333 130 114
BsiEI CG_ry'CG
Cuts at: 0 8 2127
Size: 8 2119
BsiHKAI G_wGCw'C
Cuts at: 0 56 499 1114 2127
Size: 56 443 615 1013
Fragments arranged by size:
1013 615 443 56
BsmI GAATG_Cn'
Cuts at: 0 14 2127
Size: 14 2113
Bsp1286I G_dGCh'C
Cuts at: 0 56 499 1114 1642 2127
Size: 56 443 615 528 485
Fragments arranged by size:
615 528 485 443 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 330 1230 2127
Size: 330 900 897
Fragments arranged by size:
900 897 330
BsrFI r'CCGG_y
Cuts at: 0 10 2127
Size: 10 2117
BssSI C'ACGA_G
Cuts at: 0 49 1940 2127
Size: 49 1891 187
Fragments arranged by size:
1891 187 49
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1528 1687 2127
Size: 1528 159 440
Fragments arranged by size:
1528 440 159
Bsu36I CC'TnA_GG
Cuts at: 0 1418 1823 2127
Size: 1418 405 304
Fragments arranged by size:
1418 405 304
BtsI GCAGTG_nn'
Cuts at: 0 390 684 834 999 1011 1232 1467 1757
Size: 390 294 150 165 12 221 235 290
Cuts at: 1757 2127
Size: 370
Fragments arranged by size:
390 370 294 290 235 221 165 150
12
DrdI GACnn_nn'nnGTC
Cuts at: 0 1488 2127
Size: 1488 639
EaeI y'GGCC_r
Cuts at: 0 5 89 776 1266 1355 2127
Size: 5 84 687 490 89 772
Fragments arranged by size:
772 687 490 89 84 5
EagI C'GGCC_G
Cuts at: 0 5 2127
Size: 5 2122
EarI CTCTTCn'nnn_
Cuts at: 0 147 383 811 848 1028 1119 1307 1469
Size: 147 236 428 37 180 91 188 162
Cuts at: 1469 1607 1613 2127
Size: 138 6 514
Fragments arranged by size:
514 428 236 188 180 162 147 138
91 37 6
EcoICRI GAG'CTC
Cuts at: 0 497 1112 2127
Size: 497 615 1015
Fragments arranged by size:
1015 615 497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 825 1049 1073 1100 1287 1308 1440
Size: 284 541 224 24 27 187 21 132
Cuts at: 1440 1587 1828 2127
Size: 147 241 299
Fragments arranged by size:
541 299 284 241 224 187 147 132
27 24 21
EcoO109I rG'GnC_Cy
Cuts at: 0 186 295 783 889 2127
Size: 186 109 488 106 1238
Fragments arranged by size:
1238 488 186 109 106
EcoRI G'AATT_C
Cuts at: 0 1185 2127
Size: 1185 942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 499 505 531 537 1285 1317 1423 1455
Size: 499 6 26 6 748 32 106 32
Cuts at: 1455 1468 1500 2072 2104 2127
Size: 13 32 572 32 23
Fragments arranged by size:
748 572 499 106 32 32 32 32
26 23 13 6 6
HindIII A'AGCT_T
Cuts at: 0 1273 1840 2127
Size: 1273 567 287
Fragments arranged by size:
1273 567 287
KpnI G_GTAC'C
Cuts at: 0 1178 2127
Size: 1178 949
MscI TGG'CCA
Cuts at: 0 91 778 1268 1357 2127
Size: 91 687 490 89 770
Fragments arranged by size:
770 687 490 91 89
MspA1I CmG'CkG
Cuts at: 0 1171 1204 1237 2127
Size: 1171 33 33 890
Fragments arranged by size:
1171 890 33 33
NaeI GCC'GGC
Cuts at: 0 12 2127
Size: 12 2115
NgoMIV G'CCGG_C
Cuts at: 0 10 2127
Size: 10 2117
NotI GC'GGCC_GC
Cuts at: 0 5 2127
Size: 5 2122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 968 2127
Size: 936 32 1159
Fragments arranged by size:
1159 936 32
PpuMI rG'GwC_Cy
Cuts at: 0 186 889 2127
Size: 186 703 1238
Fragments arranged by size:
1238 703 186
PspXI vC'TCGA_Gb
Cuts at: 0 608 2127
Size: 608 1519
PstI C_TGCA'G
Cuts at: 0 360 1227 1242 1497 2127
Size: 360 867 15 255 630
Fragments arranged by size:
867 630 360 255 15
PvuII CAG'CTG
Cuts at: 0 1171 1204 1237 2127
Size: 1171 33 33 890
Fragments arranged by size:
1171 890 33 33
SacI G_AGCT'C
Cuts at: 0 499 1114 2127
Size: 499 615 1013
Fragments arranged by size:
1013 615 499
SapI GCTCTTCn'nnn_
Cuts at: 0 1119 2127
Size: 1119 1008
SexAI A'CCwGG_T
Cuts at: 0 885 2127
Size: 885 1242
SfcI C'TryA_G
Cuts at: 0 159 356 894 1223 1238 1493 2127
Size: 159 197 538 329 15 255 634
Fragments arranged by size:
634 538 329 255 197 159 15
SmlI C'TyrA_G
Cuts at: 0 172 516 608 1627 2127
Size: 172 344 92 1019 500
Fragments arranged by size:
1019 500 344 172 92
StuI AGG'CCT
Cuts at: 0 244 2127
Size: 244 1883
StyI C'CwwG_G
Cuts at: 0 182 461 779 1370 1661 1805 2127
Size: 182 279 318 591 291 144 322
Fragments arranged by size:
591 322 318 291 279 182 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 2127
Size: 325 1802
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 963 1254 2127
Size: 963 291 873
Fragments arranged by size:
963 873 291
XhoI C'TCGA_G
Cuts at: 0 608 2127
Size: 608 1519
Enzymes that do cut:
AarI Acc65I AcuI AloI AlwNI ApoI AvaI BanI
BanII BbsI BciVI BglI BmrI BplI BpmI BpuEI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BssSI BstAPI Bsu36I BtsI DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HindIII
KpnI MscI MspA1I NaeI NgoMIV NotI PpiI PpuMI
PspXI PstI PvuII SacI SapI SexAI SfcI SmlI
StuI StyI TaqII XcmI XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmtI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII EciI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
MfeI MluI MmeI MslI NarI NcoI NdeI NheI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XmaI XmnI ZraI