(Circular) (Six-base) MAPSORT of: pFN21ASDA0032.seq Check: 3553 from: 1 to: 7879 pFN21ASDA0032 7879 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4036 4036 Size: 7879 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5689 278 Size: 53 83 186 936 4153 2468 Fragments arranged by size: 4153 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5222 1937 Size: 3285 4594 Acc65I G'GTAC_C Cuts at: 5200 5200 Size: 7879 AclI AA'CG_TT Cuts at: 1563 6006 6379 1563 Size: 4443 373 3063 Fragments arranged by size: 4443 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3175 4542 4788 5942 7326 7594 1632 Size: 1543 1367 246 1154 1384 268 1917 Fragments arranged by size: 1917 1543 1384 1367 1154 268 246 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7682 AflIII A'CryG_T Cuts at: 1203 2192 4359 4361 6778 7755 1203 Size: 989 2167 2 2417 977 1327 Fragments arranged by size: 2417 2167 1327 989 977 2 AgeI A'CCGG_T Cuts at: 6710 6710 Size: 7879 AhdI GACnn_n'nnGTC Cuts at: 6608 6608 Size: 7879 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7879 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2090 1184 Size: 906 6973 AlwNI CAG_nnn'CTG Cuts at: 2651 4031 5622 7194 2651 Size: 1380 1591 1572 3336 Fragments arranged by size: 3336 1591 1572 1380 ApaLI G'TGCA_C Cuts at: 5936 7092 5936 Size: 1156 6723 ApoI r'AATT_y Cuts at: 1493 4268 4685 5188 5414 5566 7675 1493 Size: 2775 417 503 226 152 2109 1697 Fragments arranged by size: 2775 2109 1697 503 417 226 152 AseI AT'TA_AT Cuts at: 160 1032 6433 160 Size: 872 5401 1606 Fragments arranged by size: 5401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 AvaI C'yCGr_G Cuts at: 1870 1943 3084 4556 5204 1870 Size: 73 1141 1472 648 4545 Fragments arranged by size: 4545 1472 1141 648 73 BamHI G'GATC_C Cuts at: 2589 5209 5576 2589 Size: 2620 367 4892 Fragments arranged by size: 4892 2620 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5200 6655 618 Size: 325 217 627 3413 1455 1842 Fragments arranged by size: 3413 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2097 2263 2623 2694 4794 5198 1391 Size: 706 166 360 71 2100 404 4072 Fragments arranged by size: 4072 2100 706 404 360 166 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3903 4072 4503 4705 928 Size: 971 2004 169 431 202 4102 Fragments arranged by size: 4102 2004 971 431 202 169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6070 6104 1903 Size: 34 4133 34 3678 Fragments arranged by size: 4133 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3625 3952 5771 6981 3625 Size: 327 1819 1210 4523 Fragments arranged by size: 4523 1819 1210 327 BclI T'GATC_A Cuts at: 1548 1548 Size: 7879 BfrBI ATG'CAT Cuts at: 2975 2975 Size: 7879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2618 6490 136 Size: 107 122 71 631 605 946 3872 1525 Fragments arranged by size: 3872 1525 946 631 605 122 107 71 BglII A'GATC_T Cuts at: 3120 7874 3120 Size: 4754 3125 BlpI GC'TnA_GC Cuts at: 2091 4178 5296 2091 Size: 2087 1118 4674 Fragments arranged by size: 4674 2087 1118 Bme1580I G_kGCm'C Cuts at: 1790 2406 3015 3035 5128 5940 7096 1790 Size: 616 609 20 2093 812 1156 2573 Fragments arranged by size: 2573 2093 1156 812 616 609 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7879 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2228 3018 3064 Size: 416 53 83 399 24 811 790 46 Cuts at: 3064 3493 4855 5610 6568 442 Size: 429 1362 755 958 1753 Fragments arranged by size: 1753 1362 958 811 790 755 429 416 399 83 53 46 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7879 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 7879 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2603 3150 3554 4118 6539 1239 Size: 1364 547 404 564 2421 2579 Fragments arranged by size: 2579 2421 1364 564 547 404 Bpu10I CC'TnA_GC Cuts at: 2533 2601 2533 Size: 68 7811 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4808 6004 6869 7167 4808 Size: 1196 865 298 5520 Fragments arranged by size: 5520 1196 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2049 3129 3672 6542 882 Size: 617 550 1080 543 2870 2219 Fragments arranged by size: 2870 2219 1080 617 550 543 BsaAI yAC'GTr Cuts at: 493 1204 4362 5176 5670 493 Size: 711 3158 814 494 2702 Fragments arranged by size: 3158 2702 814 711 494 BsaBI GATnn'nnATC Cuts at: 4338 4994 5024 7873 4338 Size: 656 30 2849 4344 Fragments arranged by size: 4344 2849 656 30 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5686 6068 6703 275 Size: 53 83 186 936 4153 382 635 1451 Fragments arranged by size: 4153 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6312 6710 6984 7131 7542 6312 Size: 398 274 147 411 6649 Fragments arranged by size: 6649 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2347 2377 4641 4671 1178 Size: 30 165 30 944 30 2264 30 4386 Fragments arranged by size: 4386 2264 944 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3147 4586 5063 1186 Size: 1961 1439 477 4002 Fragments arranged by size: 4002 1961 1439 477 BseYI C'CCAG_C Cuts at: 2661 3641 7082 7578 2661 Size: 980 3441 496 2962 Fragments arranged by size: 3441 2962 980 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2370 3738 1163 Size: 573 634 1368 5304 Fragments arranged by size: 5304 1368 634 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4987 5329 6090 7118 1540 Size: 275 186 2986 342 761 1028 2301 Fragments arranged by size: 2986 2301 1028 761 342 275 186 BsiHKAI G_wGCw'C Cuts at: 2263 4377 4794 5940 6025 7096 2263 Size: 2114 417 1146 85 1071 3046 Fragments arranged by size: 3046 2114 1146 1071 417 85 BsmI GAATG_Cn' Cuts at: 4182 5390 5483 4182 Size: 1208 93 6578 Fragments arranged by size: 6578 1208 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2263 2406 2623 2694 3015 3035 Size: 399 307 166 143 217 71 321 20 Cuts at: 3035 4377 4794 5128 5198 5940 6025 7096 1391 Size: 1342 417 334 70 742 85 1071 2174 Fragments arranged by size: 2174 1342 1071 742 417 399 334 321 307 217 166 143 85 71 70 20 BspEI T'CCGG_A Cuts at: 7542 7542 Size: 7879 BspHI T'CATG_A Cuts at: 2376 3690 4729 4930 5768 7498 2376 Size: 1314 1039 201 838 1730 2757 Fragments arranged by size: 2757 1730 1314 1039 838 201 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2082 4036 4077 5234 844 Size: 1238 1954 41 1157 3489 Fragments arranged by size: 3489 1954 1238 1157 41 BsrBI CCG'CTC Cuts at: 1394 1823 5766 1394 Size: 429 3943 3507 Fragments arranged by size: 3943 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2464 6374 6548 65 Size: 1183 1216 3910 174 1396 Fragments arranged by size: 3910 1396 1216 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4250 5059 6523 6710 1700 Size: 110 2440 809 1464 187 2869 Fragments arranged by size: 2869 2440 1464 809 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7879 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7879 BssSI C'ACGA_G Cuts at: 3881 5939 6951 3881 Size: 2058 1012 4809 Fragments arranged by size: 4809 2058 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4247 794 Size: 3453 4426 BstBI TT'CG_AA Cuts at: 2007 6683 2007 Size: 4676 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7879 BstXI CCAn_nnnn'nTGG Cuts at: 2105 3883 2105 Size: 1778 6101 BstYI r'GATC_y Cuts at: 1972 2589 3120 5209 5576 5961 5978 7430 7516 Size: 617 531 2089 367 385 17 1452 86 Cuts at: 7516 7528 7874 1972 Size: 12 346 1977 Fragments arranged by size: 2089 1977 1452 617 531 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2518 513 Size: 552 1453 5874 Fragments arranged by size: 5874 1453 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7140 Fragments arranged by size: 7140 683 56 BtsI GCAGTG_nn' Cuts at: 2406 4248 5395 6189 6209 2406 Size: 1842 1147 794 20 4076 Fragments arranged by size: 4076 1842 1147 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7844 DraI TTT'AAA Cuts at: 3100 3370 5183 3100 Size: 270 1813 5796 Fragments arranged by size: 5796 1813 270 DraIII CAC_nnn'GTG Cuts at: 2161 2161 Size: 7879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2126 6886 809 Size: 507 810 4760 1802 Fragments arranged by size: 4760 1802 810 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2488 3070 3615 Size: 54 1425 50 275 18 658 582 545 Cuts at: 3615 4110 4890 5007 5326 6215 7844 8 Size: 495 780 117 319 889 1629 43 Fragments arranged by size: 1629 1425 889 780 658 582 545 495 319 275 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5326 1537 Size: 275 3514 4090 Fragments arranged by size: 4090 3514 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2875 4799 4854 5809 1353 Size: 1522 1924 55 955 3423 Fragments arranged by size: 3423 1924 1522 955 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6466 6840 6986 387 Size: 1064 138 4877 374 146 1280 Fragments arranged by size: 4877 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2261 4792 2261 Size: 2531 5348 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2603 3150 3175 3554 4118 4542 4788 Size: 393 971 547 25 379 564 424 246 Cuts at: 4788 5942 6539 7326 7594 1239 Size: 1154 597 787 268 1524 Fragments arranged by size: 1524 1154 971 787 597 564 547 424 393 379 268 246 25 EcoNI CCTnn'n_nnAGG Cuts at: 3569 3569 Size: 7879 EcoO109I rG'GnC_Cy Cuts at: 2577 5140 2577 Size: 2563 5316 EcoRI G'AATT_C Cuts at: 5188 5566 5188 Size: 378 7501 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3139 3769 4507 4981 3139 Size: 630 738 474 6037 Fragments arranged by size: 6037 738 630 474 FspI TGC'GCA Cuts at: 6385 6385 Size: 7879 HaeII r_GCGC'y Cuts at: 1678 2826 7026 1678 Size: 1148 4200 2531 Fragments arranged by size: 4200 2531 1148 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2347 2379 2699 2731 3246 Size: 32 135 32 775 32 320 32 515 Cuts at: 3246 3278 3389 3421 3862 3894 4401 4433 6520 Size: 32 111 32 441 32 507 32 2087 Cuts at: 6520 6552 6594 6626 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2087 775 515 507 441 320 135 111 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2872 3187 5223 5469 669 Size: 988 281 934 315 2036 246 3079 Fragments arranged by size: 3079 2036 988 934 315 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7879 HpaI GTT'AAC Cuts at: 2872 5469 2872 Size: 2597 5282 KpnI G_GTAC'C Cuts at: 5204 5204 Size: 7879 MfeI C'AATT_G Cuts at: 5478 5478 Size: 7879 MluI A'CGCG_T Cuts at: 7755 7755 Size: 7879 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3308 4138 6993 7177 1305 Size: 2003 830 2855 184 2007 Fragments arranged by size: 2855 2007 2003 830 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2490 3072 3617 4112 5009 Size: 54 1425 343 658 582 545 495 897 Cuts at: 5009 10 Size: 2880 Fragments arranged by size: 2880 1425 897 658 582 545 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 3276 5837 6196 6355 518 Size: 648 1119 991 2561 359 159 2042 Fragments arranged by size: 2561 2042 1119 991 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2335 5043 5295 5972 7120 7365 1606 Size: 729 2708 252 677 1148 245 2120 Fragments arranged by size: 2708 2120 1148 729 677 252 245 NcoI C'CATG_G Cuts at: 513 1065 2518 513 Size: 552 1453 5874 Fragments arranged by size: 5874 1453 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7879 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7879 NotI GC'GGCC_GC Cuts at: 5326 5326 Size: 7879 NsiI A_TGCA'T Cuts at: 2977 2977 Size: 7879 NspI r_CATG'y Cuts at: 1132 1229 2196 2295 3842 5237 6782 1132 Size: 97 967 99 1547 1395 1545 2229 Fragments arranged by size: 2229 1547 1545 1395 967 99 97 PasI CC'CwG_GG Cuts at: 1348 3389 1348 Size: 2041 5838 PciI A'CATG_T Cuts at: 2192 6778 2192 Size: 4586 3293 PflMI CCAn_nnn'nTGG Cuts at: 1108 2311 1108 Size: 1203 6676 PfoI T'CCnGG_A Cuts at: 3570 3570 Size: 7879 PmeI GTTT'AAAC Cuts at: 5183 5183 Size: 7879 PmlI CAC'GTG Cuts at: 4362 4362 Size: 7879 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5948 5980 7461 7493 5948 Size: 32 1481 32 6334 Fragments arranged by size: 6334 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2577 2577 Size: 7879 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7879 PsiI TTA'TAA Cuts at: 5449 5449 Size: 7879 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4823 4855 4823 Size: 32 7847 PstI C_TGCA'G Cuts at: 830 2079 3322 4074 5019 5231 830 Size: 1249 1243 752 945 212 3478 Fragments arranged by size: 3478 1249 1243 945 752 212 PvuI CG_AT'CG Cuts at: 2001 4987 2001 Size: 2986 4893 PvuII CAG'CTG Cuts at: 2335 2335 Size: 7879 SacI G_AGCT'C Cuts at: 2263 4794 2263 Size: 2531 5348 SalI G'TCGA_C Cuts at: 1936 5221 1936 Size: 3285 4594 SapI GCTCTTCn'nnn_ Cuts at: 4799 4799 Size: 7879 SbfI CC_TGCA'GG Cuts at: 2079 5231 2079 Size: 3152 4727 ScaI AGT'ACT Cuts at: 3651 4785 5054 6127 7839 3651 Size: 1134 269 1073 1712 3691 Fragments arranged by size: 3691 1712 1134 1073 269 SexAI A'CCwGG_T Cuts at: 2056 2056 Size: 7879 SfcI C'TryA_G Cuts at: 826 1045 2075 3318 4037 4070 5015 5227 6362 Size: 219 1030 1243 719 33 945 212 1135 Cuts at: 6362 7043 7234 826 Size: 681 191 1471 Fragments arranged by size: 1471 1243 1135 1030 945 719 681 219 212 191 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7879 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7879 SmaI CCC'GGG Cuts at: 1872 4558 5206 1872 Size: 2686 648 4545 Fragments arranged by size: 4545 2686 648 SmlI C'TyrA_G Cuts at: 820 1017 1943 4787 5983 6884 7146 820 Size: 197 926 2844 1196 901 262 1553 Fragments arranged by size: 2844 1553 1196 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5176 5670 493 Size: 4683 494 2702 Fragments arranged by size: 4683 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7879 SphI G_CATG'C Cuts at: 1132 5237 1132 Size: 4105 3774 SspI AAT'ATT Cuts at: 5 52 2439 3686 5803 5 Size: 47 2387 1247 2117 2081 Fragments arranged by size: 2387 2117 2081 1247 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7879 StyI C'CwwG_G Cuts at: 513 1065 2208 2518 3606 5318 513 Size: 552 1143 310 1088 1712 3074 Fragments arranged by size: 3074 1712 1143 1088 552 310 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2340 4531 5906 5923 6076 6261 6706 Size: 1255 760 2191 1375 17 153 185 445 Cuts at: 6706 325 Size: 1498 Fragments arranged by size: 2191 1498 1375 1255 760 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3148 3649 4783 Size: 274 80 33 51 1443 1171 501 1134 Cuts at: 4783 5052 5100 6125 7837 96 Size: 269 48 1025 1712 138 Fragments arranged by size: 1712 1443 1171 1134 1025 501 274 269 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7839 7871 7839 Size: 32 7847 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7879 XbaI T'CTAG_A Cuts at: 2887 3457 5215 2887 Size: 570 1758 5551 Fragments arranged by size: 5551 1758 570 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2215 2525 3625 5002 2215 Size: 310 1100 1377 5092 Fragments arranged by size: 5092 1377 1100 310 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7879 XmaI C'CCGG_G Cuts at: 1870 4556 5204 1870 Size: 2686 648 4545 Fragments arranged by size: 4545 2686 648 XmnI GAAnn'nnTTC Cuts at: 2543 3686 4079 4272 4926 6008 2543 Size: 1143 393 193 654 1082 4414 Fragments arranged by size: 4414 1143 1082 654 393 193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5687 276 Size: 53 83 186 936 4153 2468 Fragments arranged by size: 4153 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI