Restriction map for
pFN21ASDA0031
(Circular) (Six-base) MAPSORT of: pFN21ASDA0031.seq Check: 718 from: 1 to: 7645
pFN21ASDA0031 7645 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5455 278
Size: 53 83 186 936 3919 2468
Fragments arranged by size:
3919 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4470 4988 1937
Size: 2533 518 4594
Fragments arranged by size:
4594 2533 518
Acc65I G'GTAC_C
Cuts at: 3630 4966 3630
Size: 1336 6309
AclI AA'CG_TT
Cuts at: 1563 5772 6145 1563
Size: 4209 373 3063
Fragments arranged by size:
4209 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2843 3266 5708 7092 7360 1632
Size: 1211 423 2442 1384 268 1917
Fragments arranged by size:
2442 1917 1384 1211 423 268
AfeI AGC'GCT
Cuts at: 2501 2501
Size: 7645
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7448
AflIII A'CryG_T
Cuts at: 1203 2450 2644 3318 6544 7521 1203
Size: 1247 194 674 3226 977 1327
Fragments arranged by size:
3226 1327 1247 977 674 194
AgeI A'CCGG_T
Cuts at: 3383 6476 3383
Size: 3093 4552
AhdI GACnn_n'nnGTC
Cuts at: 6374 6374
Size: 7645
AleI CACnn'nnGTG
Cuts at: 1166 2576 3881 1166
Size: 1410 1305 4930
Fragments arranged by size:
4930 1410 1305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7645
AlwNI CAG_nnn'CTG
Cuts at: 2783 2970 5388 6960 2783
Size: 187 2418 1572 3468
Fragments arranged by size:
3468 2418 1572 187
ApaI G_GGCC'C
Cuts at: 4355 4355
Size: 7645
ApaLI G'TGCA_C
Cuts at: 5702 6858 5702
Size: 1156 6489
ApoI r'AATT_y
Cuts at: 1493 2324 2650 3035 4817 4874 4954 5180 5332
Size: 831 326 385 1782 57 80 226 152
Cuts at: 5332 7441 1493
Size: 2109 1697
Fragments arranged by size:
2109 1782 1697 831 385 326 226 152
80 57
AseI AT'TA_AT
Cuts at: 160 1032 2885 3024 6199 160
Size: 872 1853 139 3175 1606
Fragments arranged by size:
3175 1853 1606 872 139
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7645
AvaI C'yCGr_G
Cuts at: 1870 1943 3726 4812 4970 1870
Size: 73 1783 1086 158 4545
Fragments arranged by size:
4545 1783 1086 158 73
BamHI G'GATC_C
Cuts at: 4975 5342 4975
Size: 367 7278
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3353 3518 3630 4966 6421
Size: 325 217 627 1566 165 112 1336 1455
Cuts at: 6421 618
Size: 1842
Fragments arranged by size:
1842 1566 1455 1336 627 325 217 165
112
BanII G_rGCy'C
Cuts at: 1391 2406 2987 3927 4355 4422 4964 1391
Size: 1015 581 940 428 67 542 4072
Fragments arranged by size:
4072 1015 940 581 542 428 67
BbeI G_GCGC'C
Cuts at: 3357 3357
Size: 7645
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 3588 3710 4714 928
Size: 971 381 1308 122 1004 3859
Fragments arranged by size:
3859 1308 1004 971 381 122
BbvCI CC'TCA_GC
Cuts at: 2718 2718
Size: 7645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4861 4895 5836 5870 1903
Size: 34 2924 34 941 34 3678
Fragments arranged by size:
3678 2924 941 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2020 5537 6747 2020
Size: 3517 1210 2918
Fragments arranged by size:
3517 2918 1210
BclI T'GATC_A
Cuts at: 1548 2748 3688 4824 1548
Size: 1200 940 1136 4369
Fragments arranged by size:
4369 1200 1136 940
BfrBI ATG'CAT
Cuts at: 3946 3946
Size: 7645
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6256 136
Size: 107 122 71 631 605 4584 1525
Fragments arranged by size:
4584 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2116 2512 2997 7640 2116
Size: 396 485 4643 2121
Fragments arranged by size:
4643 2121 485 396
BlpI GC'TnA_GC
Cuts at: 4089 5062 4089
Size: 973 6672
Bme1580I G_kGCm'C
Cuts at: 1790 3917 4355 4590 5706 6862 1790
Size: 2127 438 235 1116 1156 2573
Fragments arranged by size:
2573 2127 1156 1116 438 235
BmgBI CAC'GTC
Cuts at: 1318 3184 1318
Size: 1866 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2953 3560 3676
Size: 416 53 83 399 24 1536 607 116
Cuts at: 3676 3927 3989 5376 6334 442
Size: 251 62 1387 958 1753
Fragments arranged by size:
1753 1536 1387 958 607 416 399 251
116 83 62 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7645
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3601 3601
Size: 7645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2622 2837 2935 3590 6305 1239
Size: 1383 215 98 655 2715 2579
Fragments arranged by size:
2715 2579 1383 655 215 98
Bpu10I CC'TnA_GC
Cuts at: 2718 3269 2718
Size: 551 7094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4022 4118 5770 6635 6933 4022
Size: 96 1652 865 298 4734
Fragments arranged by size:
4734 1652 865 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 2249 6308 882
Size: 617 603 147 4059 2219
Fragments arranged by size:
4059 2219 617 603 147
BsaAI yAC'GTr
Cuts at: 493 1204 4146 4591 4942 5436 493
Size: 711 2942 445 351 494 2702
Fragments arranged by size:
2942 2702 711 494 445 351
BsaBI GATnn'nnATC
Cuts at: 3977 4194 7639 3977
Size: 217 3445 3983
Fragments arranged by size:
3983 3445 217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3354 5452 5834 6469
Size: 53 83 186 936 1821 2098 382 635
Cuts at: 6469 275
Size: 1451
Fragments arranged by size:
2098 1821 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3383 4683 6078 6476 6750 6897 7308 3383
Size: 1300 1395 398 274 147 411 3720
Fragments arranged by size:
3720 1395 1300 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7420
Fragments arranged by size:
7420 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2246 3254 3752 3902 4485 4791 1186
Size: 1060 1008 498 150 583 306 4040
Fragments arranged by size:
4040 1060 1008 583 498 306 150
BseYI C'CCAG_C
Cuts at: 2980 3530 4162 6848 7344 2980
Size: 550 632 2686 496 3281
Fragments arranged by size:
3281 2686 632 550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2783 3347 4409 1163
Size: 573 1047 564 1062 4399
Fragments arranged by size:
4399 1062 1047 573 564
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5095 5856 6884 1540
Size: 275 186 3094 761 1028 2301
Fragments arranged by size:
3094 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2406 3375 3927 4926 5706 5791 6862 2406
Size: 969 552 999 780 85 1071 3189
Fragments arranged by size:
3189 1071 999 969 780 552 85
BsmI GAATG_Cn'
Cuts at: 5156 5249 5156
Size: 93 7552
BsmBI CGTCTCn'nnnn_
Cuts at: 4007 4555 4007
Size: 548 7097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2406 2987 3375 3917 3927 4355 4422
Size: 399 616 581 388 542 10 428 67
Cuts at: 4422 4590 4926 4964 5706 5791 6862 1391
Size: 168 336 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 616 581 542 428 399
388 336 168 85 67 38 10
BspEI T'CCGG_A
Cuts at: 4683 7308 4683
Size: 2625 5020
BspHI T'CATG_A
Cuts at: 2164 4821 5534 7264 2164
Size: 2657 713 1730 2545
Fragments arranged by size:
2657 2545 1730 713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5000 844
Size: 4156 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2743 5532 1394
Size: 429 920 2789 3507
Fragments arranged by size:
3507 2789 920 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3739 6140 6314 65
Size: 1183 2491 2401 174 1396
Fragments arranged by size:
2491 2401 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2805 3383 6289 6476 1700
Size: 110 995 578 2906 187 2869
Fragments arranged by size:
2906 2869 995 578 187 110
BsrGI T'GTAC_A
Cuts at: 96 3465 4626 96
Size: 3369 1161 3115
Fragments arranged by size:
3369 3115 1161
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7645
BssSI C'ACGA_G
Cuts at: 5705 6717 5705
Size: 1012 6633
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2761 3952 4741 794
Size: 1967 1191 789 3698
Fragments arranged by size:
3698 1967 1191 789
BstBI TT'CG_AA
Cuts at: 2007 6449 2007
Size: 4442 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7645
BstXI CCAn_nnnn'nTGG
Cuts at: 3883 4255 3883
Size: 372 7273
BstYI r'GATC_y
Cuts at: 1972 2116 2383 2512 2997 3159 3615 4195 4392
Size: 144 267 129 485 162 456 580 197
Cuts at: 4392 4975 5342 5727 5744 7196 7282 7294 7640
Size: 583 367 385 17 1452 86 12 346
Cuts at: 7640 1972
Size: 1977
Fragments arranged by size:
1977 1452 583 580 485 456 385 367
346 267 197 162 144 129 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2247 2247
Size: 7645
BtgI C'CryG_G
Cuts at: 513 1065 2022 2440 3200 3600 513
Size: 552 957 418 760 400 4558
Fragments arranged by size:
4558 957 760 552 418 400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6906
Fragments arranged by size:
6906 683 56
BtsI GCAGTG_nn'
Cuts at: 5161 5955 5975 5161
Size: 794 20 6831
Fragments arranged by size:
6831 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7645
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7610
DraI TTT'AAA
Cuts at: 4949 4949
Size: 7645
DraIII CAC_nnn'GTG
Cuts at: 2787 2866 2787
Size: 79 7566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3998 6652 809
Size: 507 2682 2654 1802
Fragments arranged by size:
2682 2654 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3502 3624 4572
Size: 54 1425 50 275 18 1672 122 948
Cuts at: 4572 4650 5092 5981 7610 8
Size: 78 442 889 1629 43
Fragments arranged by size:
1672 1629 1425 948 889 442 275 122
78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5092 1537
Size: 275 3280 4090
Fragments arranged by size:
4090 3280 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2540 2624 4299 5575 1353
Size: 1187 84 1675 1276 3423
Fragments arranged by size:
3423 1675 1276 1187 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2396 3248 4766 6232 6606 6752
Size: 1064 138 807 852 1518 1466 374 146
Cuts at: 6752 387
Size: 1280
Fragments arranged by size:
1518 1466 1280 1064 852 807 374 146
138
EcoICRI GAG'CTC
Cuts at: 2404 3925 2404
Size: 1521 6124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2622 2837 2843 2935 3266 3590 5708
Size: 393 990 215 6 92 331 324 2118
Cuts at: 5708 6305 7092 7360 1239
Size: 597 787 268 1524
Fragments arranged by size:
2118 1524 990 787 597 393 331 324
268 215 92 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3167 3233 3268 3407 3845 4112 3167
Size: 66 35 139 438 267 6700
Fragments arranged by size:
6700 438 267 139 66 35
EcoO109I rG'GnC_Cy
Cuts at: 3100 4105 4351 4352 4534 4846 3100
Size: 1005 246 1 182 312 5899
Fragments arranged by size:
5899 1005 312 246 182 1
EcoRI G'AATT_C
Cuts at: 4817 4954 5332 4817
Size: 137 378 7130
Fragments arranged by size:
7130 378 137
FspI TGC'GCA
Cuts at: 2681 3845 6151 2681
Size: 1164 2306 4175
Fragments arranged by size:
4175 2306 1164
HaeII r_GCGC'y
Cuts at: 1678 2503 3357 6792 1678
Size: 825 854 3435 2531
Fragments arranged by size:
3435 2531 854 825
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2101 2133 4535 4567 6286
Size: 32 135 32 529 32 2402 32 1719
Cuts at: 6286 6318 6360 6392 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2402 1719 529 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3808 3853 4989 5235 669
Size: 988 281 1870 45 1136 246 3079
Fragments arranged by size:
3079 1870 1136 988 281 246 45
HindIII A'AGCT_T
Cuts at: 748 4638 748
Size: 3890 3755
HpaI GTT'AAC
Cuts at: 5235 5235
Size: 7645
KasI G'GCGC_C
Cuts at: 3353 3353
Size: 7645
KpnI G_GTAC'C
Cuts at: 3634 4970 3634
Size: 1336 6309
MfeI C'AATT_G
Cuts at: 3696 5244 3696
Size: 1548 6097
MluI A'CGCG_T
Cuts at: 7521 7521
Size: 7645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2858 3423 3778 6759 6943 1305
Size: 1553 565 355 2981 184 2007
Fragments arranged by size:
2981 2007 1553 565 355 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3626 4574 10
Size: 54 1425 343 1794 948 3081
Fragments arranged by size:
3081 1794 1425 948 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2169 2333 2576 3323 3641 3881 3983
Size: 648 1003 164 243 747 318 240 102
Cuts at: 3983 4256 5603 5962 6121 518
Size: 273 1347 359 159 2042
Fragments arranged by size:
2042 1347 1003 747 648 359 318 273
243 240 164 159 102
MspA1I CmG'CkG
Cuts at: 1606 2672 5061 5738 6886 7131 1606
Size: 1066 2389 677 1148 245 2120
Fragments arranged by size:
2389 2120 1148 1066 677 245
NarI GG'CG_CC
Cuts at: 3354 3354
Size: 7645
NcoI C'CATG_G
Cuts at: 513 1065 2022 2440 513
Size: 552 957 418 5718
Fragments arranged by size:
5718 957 552 418
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7645
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7645
NotI GC'GGCC_GC
Cuts at: 5092 5092
Size: 7645
NsiI A_TGCA'T
Cuts at: 3948 3948
Size: 7645
NspI r_CATG'y
Cuts at: 1132 1229 2454 2648 2759 2896 3322 3905 5003
Size: 97 1225 194 111 137 426 583 1098
Cuts at: 5003 6548 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1225 1098 583 426 194 137
111 97
PasI CC'CwG_GG
Cuts at: 1348 2180 4696 1348
Size: 832 2516 4297
Fragments arranged by size:
4297 2516 832
PciI A'CATG_T
Cuts at: 2450 2644 3318 6544 2450
Size: 194 674 3226 3551
Fragments arranged by size:
3551 3226 674 194
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2866 1108
Size: 1758 5887
PfoI T'CCnGG_A
Cuts at: 2638 2814 4395 2638
Size: 176 1581 5888
Fragments arranged by size:
5888 1581 176
PmeI GTTT'AAAC
Cuts at: 4949 4949
Size: 7645
PmlI CAC'GTG
Cuts at: 4591 4591
Size: 7645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5714 5746 7227 7259 5714
Size: 32 1481 32 6100
Fragments arranged by size:
6100 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2585 1613
Size: 972 6673
PsiI TTA'TAA
Cuts at: 3156 5215 3156
Size: 2059 5586
PspOMI G'GGCC_C
Cuts at: 4351 4351
Size: 7645
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2011 2043 2011
Size: 32 7613
PstI C_TGCA'G
Cuts at: 830 4543 4558 4997 830
Size: 3713 15 439 3478
Fragments arranged by size:
3713 3478 439 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7645
PvuII CAG'CTG
Cuts at: 2672 2672
Size: 7645
SacI G_AGCT'C
Cuts at: 2406 3927 2406
Size: 1521 6124
SalI G'TCGA_C
Cuts at: 1936 4987 1936
Size: 3051 4594
SapI GCTCTTCn'nnn_
Cuts at: 4299 4299
Size: 7645
SbfI CC_TGCA'GG
Cuts at: 4997 4997
Size: 7645
ScaI AGT'ACT
Cuts at: 2216 5893 7605 2216
Size: 3677 1712 2256
Fragments arranged by size:
3677 2256 1712
SfcI C'TryA_G
Cuts at: 826 1045 4539 4554 4993 6128 6809 7000 826
Size: 219 3494 15 439 1135 681 191 1471
Fragments arranged by size:
3494 1471 1135 681 439 219 191 15
SfoI GGC'GCC
Cuts at: 3355 3355
Size: 7645
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7645
SgrAI Cr'CCGG_yG
Cuts at: 1700 3383 1700
Size: 1683 5962
SmaI CCC'GGG
Cuts at: 1872 4814 4972 1872
Size: 2942 158 4545
Fragments arranged by size:
4545 2942 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4037 4097 5749 6650 6912 820
Size: 197 926 2094 60 1652 901 262 1553
Fragments arranged by size:
2094 1652 1553 926 901 262 197 60
SnaBI TAC'GTA
Cuts at: 493 4942 5436 493
Size: 4449 494 2702
Fragments arranged by size:
4449 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7645
SphI G_CATG'C
Cuts at: 1132 2759 5003 1132
Size: 1627 2244 3774
Fragments arranged by size:
3774 2244 1627
SspI AAT'ATT
Cuts at: 5 52 5569 5
Size: 47 5517 2081
Fragments arranged by size:
5517 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7645
StyI C'CwwG_G
Cuts at: 513 1065 2022 2440 3879 4782 5084 513
Size: 552 957 418 1439 903 302 3074
Fragments arranged by size:
3074 1439 957 903 552 418 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4621 4709 5672 5689 5842 6027 6472
Size: 1255 3041 88 963 17 153 185 445
Cuts at: 6472 325
Size: 1498
Fragments arranged by size:
3041 1498 1255 963 445 185 153 88
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 2961 3465
Size: 274 80 33 51 1443 237 747 504
Cuts at: 3465 3963 4626 5891 7603 96
Size: 498 663 1265 1712 138
Fragments arranged by size:
1712 1443 1265 747 663 504 498 274
237 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7605 7637 7605
Size: 32 7613
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7645
XbaI T'CTAG_A
Cuts at: 4981 4981
Size: 7645
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4255 4255
Size: 7645
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7645
XmaI C'CCGG_G
Cuts at: 1870 4812 4970 1870
Size: 2942 158 4545
Fragments arranged by size:
4545 2942 158
XmnI GAAnn'nnTTC
Cuts at: 4371 5774 4371
Size: 1403 6242
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5453 276
Size: 53 83 186 936 3919 2468
Fragments arranged by size:
3919 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BsiWI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PpuMI
PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI