Restriction map for
pFN21ASDA0026
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstZ17I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AhdI 126 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 113 CCTCAGG CC^T[ACGT]AGG
ApaI 305 GGGCCC GGGCC^C
AspEI 126 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 671 GAGCAC G[AT]GC[AT]^C
AvaI 308 CCCGGG C^[CT]CG[GA]G
AvaII 133 GGTCC G^G[AT]CC
BamHI 208 GGATCC G^GATCC
BanII 305 GGGCCC G[GA]GC[CT]^C
BbvCI 283 CCTCAGC CCTCAGC(-5/-2)
BfaI 74 CTAG C^TAG
BpmI 475 CTGGAG CTGGAG(16/14)
BsiHKAI 671 GAGCAC G[AT]GC[AT]^C
BsoBI 308 CCCGGG C^[CT]CG[GA]G
BsrDI 709 GCAATG GCAATG(2/0)
BssSI 29 CTCGTG CACGAG(-5/-1)
BstYI 208 GGATCC [GA]^GATC[CT]
Bsu36I 113 CCTCAGG CC^T[ACGT]AGG
DpnII 209 GATC ^GATC
DsaI 391 CCATGG C^C[GA][CT]GG
Eam1105I 126 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 52 GAAGAG CTCTTC(1/4)
Eco81I 113 CCTCAGG CC^T[ACGT]AGG
EcoRI 795 GAATTC G^AATTC
EcoT14I 391 CCATGG C^C[AT][AT]GG
HapII 309 CCGG C^CGG
HpaII 309 CCGG C^CGG
HphI 639 TCACC GGTGA(8/7)
Ksp632I 52 GAAGAG CTCTTC(1/4)
MaeI 74 CTAG C^TAG
MaeIII 418 GTTAC ^GT[ACGT]AC
MboI 209 GATC ^GATC
MflI 208 GGATCC [GA]^GATC[CT]
MslI 640 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 309 CCGG C^CGG
NcoI 391 CCATGG C^CATGG
NdeII 209 GATC ^GATC
NspI 83 ACATGC [GA]CATG^[CT]
PleI 243 GACTC GAGTC(4/5)
Sau3AI 209 GATC ^GATC
SmaI 308 CCCGGG CCC^GGG
SspI 563 AATATT AAT^ATT
StyI 391 CCATGG C^C[AT][AT]GG
TfiI 372 GATTC G^A[AT]TC
XhoII 208 GGATCC [GA]^GATC[CT]
XmaCI 308 CCCGGG C^CCGGG
XmaI 308 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 852 CCGC CCGC(-3/-1)
532 GCGG
AcsI 601 AAATTT [GA]^AATT[CT]
795 GAATTC
AfaI 269 GTAC GT^AC
846 GTAC
AlwI 209 GATCC GGATC(4/5)
208 GGATC
ApoI 601 AAATTT [GA]^AATT[CT]
795 GAATTC
BbvI 432 GCAGC GCAGC(8/12)
591 GCTGC
BcnI 308 CCCGG CC^[GC]GG
309 CCGGG
BsiYI 199 CCCCCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
247 CCTGGAAACGG
BslI 199 CCCCCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
247 CCTGGAAACGG
BsmFI 410 GGGAC GGGAC(10/14)
657 GTCCC
BstXI 166 CCAGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
660 CCAGGTTTATGG
Fnu4HI 432 GCAGC GC^[ACGT]GC
591 GCTGC
HinfI 243 GACTC G^A[ACGT]TC
372 GATTC
ItaI 432 GCAGC GC^[ACGT]GC
591 GCTGC
NciI 308 CCCGG CC^[GC]GG
309 CCGGG
PstI 44 CTGCAG CTGCA^G
188 CTGCAG
RsaI 269 GTAC GT^AC
846 GTAC
SfcI 44 CTGCAG C^T[GA][CT]AG
188 CTGCAG
TseI 432 GCAGC G^C[AT]GC
591 GCTGC
TspRI 321 CACTG CA[GC]TG[ACGT][ACGT]^
819 CAGTG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI BbsI BmyI BpuAI BsaJI
BseRI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI DraII EcoO109I EcoRII
HaeIII MboII MnlI MseI MspA1I
MvaI MwoI NlaIII NlaIV PvuII
Sau96I ScrFI SfaNI Tru9I Tsp509I