Restriction map for
pFN21ASDA0025
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 130 GTCTAC GT^[AC][GT]AC
AcsI 726 AAATTT [GA]^AATT[CT]
Alw44I 599 GTGCAC G^TGCAC
AlwNI 560 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 349 CCTGAGG CC^T[ACGT]AGG
ApaLI 599 GTGCAC G^TGCAC
ApoI 726 AAATTT [GA]^AATT[CT]
Asp700I 385 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI 1069 CTAG C^TAG
BsaWI 478 TCCGGT [AT]^CCGG[AT]
BsgI 623 CTGCAC GTGCAG(16/14)
BsiEI 84 CGACCG CG[GA][CT]^CG
BsrDI 257 GCAATG GCAATG(2/0)
BsrFI 939 GCCGGT [GA]^CCGG[CT]
BsrI 651 CCAGT ACTGG(1/-1)
Bsu36I 349 CCTGAGG CC^T[ACGT]AGG
CfoI 57 GCGC GCG^C
Cfr10I 939 GCCGGT [GA]^CCGG[CT]
DsaI 87 CCGCGG C^C[GA][CT]GG
Eco81I 349 CCTGAGG CC^T[ACGT]AGG
HaeII 56 AGCGCC [GA]GCGC^[CT]
HhaI 57 GCGC GCG^C
HinP1I 57 GCGC G^CGC
KspI 87 CCGCGG CCGC^GG
MaeI 1069 CTAG C^TAG
MslI 873 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PvuII 659 CAGCTG CAG^CTG
SacII 87 CCGCGG CCGC^GG
SapI 42 GAAGAGC GCTCTTC(1/4)
ScaI 539 AGTACT AGT^ACT
SspI 551 AATATT AAT^ATT
TaqI 783 TCGA T^CGA
Tsp45I 28 GTCAC ^GT[GC]AC
TthHB8I 783 TCGA T^CGA
XmnI 385 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AlwI 1021 GATCC GGATC(4/5)
192 GGATC
AspHI 599 GTGCAC G[AT]GC[AT]^C
1101 GAGCAC
AvaI 19 CCCGAG C^[CT]CG[GA]G
136 CCCGAG
BanII 22 GAGCCC G[GA]GC[CT]^C
45 GAGCCC
BbsI 1033 GAAGAC GAAGAC(2/6)
1117 GTCTTC
BciVI 345 GTATCC GTATCC(6/5)
836 GTATCC
BglI 295 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
442 GCCCAGCAGGC
BpuAI 1033 GAAGAC GAAGAC(2/6)
1117 GTCTTC
BsaI 10 GAGACC GGTCTC(1/5)
618 GGTCTC
BsiHKAI 599 GTGCAC G[AT]GC[AT]^C
1101 GAGCAC
BsmFI 209 GGGAC GGGAC(10/14)
339 GGGAC
BsoBI 19 CCCGAG C^[CT]CG[GA]G
136 CCCGAG
BspMI 627 ACCTGC ACCTGC(4/8)
902 ACCTGC
BstAPI 237 GCATGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
559 GCAGCCACTGC
DpnII 193 GATC ^GATC
1021 GATC
DraIII 593 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
743 CACAAGGTG
EcoT14I 296 CCAAGG C^C[AT][AT]GG
456 CCAAGG
HapII 479 CCGG C^CGG
940 CCGG
HpaII 479 CCGG C^CGG
940 CCGG
HphI 39 GGTGA GGTGA(8/7)
195 TCACC
MaeII 911 ACGT A^CGT
977 ACGT
MaeIII 28 GTCAC ^GT[ACGT]AC
482 GTTAC
MboI 193 GATC ^GATC
1021 GATC
MspA1I 87 CCGCGG C[AC]G^C[GT]G
659 CAGCTG
MspI 479 CCGG C^CGG
940 CCGG
NdeII 193 GATC ^GATC
1021 GATC
NspI 237 GCATGT [GA]CATG^[CT]
545 ACATGC
PflMI 456 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
955 CCAAATGATGG
PleI 4 GAGTC GAGTC(4/5)
267 GAGTC
PpuMI 465 GGGTCCT [GA]G^G[AT]CC[CT]
989 AGGACCC
Sau3AI 193 GATC ^GATC
1021 GATC
StuI 430 AGGCCT AGG^CCT
459 AGGCCT
StyI 296 CCAAGG C^C[AT][AT]GG
456 CCAAGG
Tsp509I 168 AATT ^AATT
727 AATT
TspRI 564 CACTG CA[GC]TG[ACGT][ACGT]^
1017 CACTG
Van91I 456 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
955 CCAAATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
Cfr13I DdeI DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HgaI
HinfI ItaI Ksp632I MboII MnlI
MseI MvaI MvnI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
SmlI TfiI Tru9I TseI