(Circular) (Six-base) MAPSORT of: pFN21ASDA0025.seq Check: 8345 from: 1 to: 5902 pFN21ASDA0025 5902 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2659 2659 Size: 5902 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 3245 1937 Size: 217 1091 4594 Fragments arranged by size: 4594 1091 217 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 2510 3075 3965 5349 5617 1632 Size: 846 32 565 890 1384 268 1917 Fragments arranged by size: 1917 1384 890 846 565 268 32 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2568 1184 Size: 1384 4518 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2411 2443 2411 Size: 32 5870 AlwNI CAG_nnn'CTG Cuts at: 2588 3645 5217 2588 Size: 1057 1572 3273 Fragments arranged by size: 3273 1572 1057 ApaLI G'TGCA_C Cuts at: 2622 3959 5115 2622 Size: 1337 1156 3409 Fragments arranged by size: 3409 1337 1156 ApoI r'AATT_y Cuts at: 1493 2749 3211 3437 3589 5698 1493 Size: 1256 462 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 462 226 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2159 3227 1870 Size: 73 99 117 1068 4545 Fragments arranged by size: 4545 1068 117 99 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3223 4678 618 Size: 325 217 627 1436 1455 1842 Fragments arranged by size: 1842 1455 1436 627 325 217 BanII G_rGCy'C Cuts at: 1391 2049 2072 3221 1391 Size: 658 23 1149 4072 Fragments arranged by size: 4072 1149 658 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3063 3133 928 Size: 971 1164 70 3697 Fragments arranged by size: 3697 1164 971 70 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2379 2870 3794 5004 2379 Size: 491 924 1210 3277 Fragments arranged by size: 3277 1210 924 491 BclI T'GATC_A Cuts at: 1548 1548 Size: 5902 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2324 2471 4513 Size: 107 122 71 631 605 652 147 2042 Cuts at: 4513 136 Size: 1525 Fragments arranged by size: 2042 1525 652 631 605 147 122 107 71 BglII A'GATC_T Cuts at: 5897 5897 Size: 5902 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 2626 3963 5119 1790 Size: 836 1337 1156 2573 Fragments arranged by size: 2573 1337 1156 836 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3633 4591 442 Size: 416 53 83 399 24 2216 958 1753 Fragments arranged by size: 2216 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2398 2398 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2137 2640 2757 3060 4562 1239 Size: 898 503 117 303 1502 2579 Fragments arranged by size: 2579 1502 898 503 303 117 Bpu10I CC'TnA_GC Cuts at: 2144 2882 2144 Size: 738 5164 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2117 2368 2434 2703 4027 4892 5190 2117 Size: 251 66 269 1324 865 298 2829 Fragments arranged by size: 2829 1324 865 298 269 251 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2027 2647 4565 882 Size: 617 528 620 1918 2219 Fragments arranged by size: 2219 1918 620 617 528 BsaAI yAC'GTr Cuts at: 493 1204 3199 3693 493 Size: 711 1995 494 2702 Fragments arranged by size: 2702 1995 711 494 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3709 4091 4726 275 Size: 53 83 186 936 2176 382 635 1451 Fragments arranged by size: 2176 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2501 4335 4733 5007 5154 5565 2501 Size: 1834 398 274 147 411 2838 Fragments arranged by size: 2838 1834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2858 2888 1178 Size: 30 165 30 1455 30 4192 Fragments arranged by size: 4192 1455 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2706 2866 2982 1186 Size: 1168 352 160 116 4106 Fragments arranged by size: 4106 1168 352 160 116 BseYI C'CCAG_C Cuts at: 2466 3181 5105 5601 2466 Size: 715 1924 496 2767 Fragments arranged by size: 2767 1924 715 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2631 1163 Size: 573 895 4434 Fragments arranged by size: 4434 895 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2110 3352 4113 5141 1540 Size: 275 186 109 1242 761 1028 2301 Fragments arranged by size: 2301 1242 1028 761 275 186 109 BsiHKAI G_wGCw'C Cuts at: 2626 3128 3963 4048 5119 2626 Size: 502 835 85 1071 3409 Fragments arranged by size: 3409 1071 835 502 85 BsmI GAATG_Cn' Cuts at: 2474 2713 2767 3413 3506 2474 Size: 239 54 646 93 4870 Fragments arranged by size: 4870 646 239 93 54 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2072 2626 3128 3221 3963 4048 Size: 399 259 23 554 502 93 742 85 Cuts at: 4048 5119 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 554 502 399 259 93 85 23 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2659 2934 3257 844 Size: 1815 275 323 3489 Fragments arranged by size: 3489 1815 323 275 BsrBI CCG'CTC Cuts at: 1394 1823 3789 1394 Size: 429 1966 3507 Fragments arranged by size: 3507 1966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2287 4397 4571 65 Size: 1183 1039 2110 174 1396 Fragments arranged by size: 2110 1396 1183 1039 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2962 4546 4733 1700 Size: 110 1152 1584 187 2869 Fragments arranged by size: 2869 1584 1152 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 2588 794 Size: 1472 322 4108 Fragments arranged by size: 4108 1472 322 BstBI TT'CG_AA Cuts at: 2007 4706 2007 Size: 2699 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 3232 3599 3984 4001 5453 5539 5551 5897 Size: 1260 367 385 17 1452 86 12 346 Cuts at: 5897 1972 Size: 1977 Fragments arranged by size: 1977 1452 1260 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2373 2373 Size: 5902 BtgI C'CryG_G Cuts at: 513 1065 2022 2110 513 Size: 552 957 88 4305 Fragments arranged by size: 4305 957 552 88 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3172 487 Size: 683 56 1946 3217 Fragments arranged by size: 3217 1946 683 56 BtsI GCAGTG_nn' Cuts at: 2586 3418 4212 4232 2586 Size: 832 794 20 4256 Fragments arranged by size: 4256 832 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5867 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DraIII CAC_nnn'GTG Cuts at: 2621 2771 2621 Size: 150 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3349 4238 5867 Size: 54 1425 50 275 18 1519 889 1629 Cuts at: 5867 8 Size: 43 Fragments arranged by size: 1629 1519 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3349 1537 Size: 275 1537 4090 Fragments arranged by size: 4090 1537 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2282 3168 3832 1353 Size: 707 222 886 664 3423 Fragments arranged by size: 3423 886 707 664 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2137 2478 2510 2640 2757 3060 3075 Size: 393 505 341 32 130 117 303 15 Cuts at: 3075 3965 4562 5349 5617 1239 Size: 890 597 787 268 1524 Fragments arranged by size: 1524 890 787 597 505 393 341 303 268 130 117 32 15 EcoO109I rG'GnC_Cy Cuts at: 2489 3013 3176 2489 Size: 524 163 5215 Fragments arranged by size: 5215 524 163 EcoRI G'AATT_C Cuts at: 3211 3589 3211 Size: 378 5524 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3169 3169 Size: 5902 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2083 5049 1678 Size: 405 2966 2531 Fragments arranged by size: 2966 2531 405 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4543 4575 4617 4649 1373 Size: 32 135 32 2971 32 42 32 2626 Fragments arranged by size: 2971 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3246 3492 669 Size: 988 281 1308 246 3079 Fragments arranged by size: 3079 1308 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 2796 5016 5200 1305 Size: 888 603 2220 184 2007 Fragments arranged by size: 2220 2007 888 603 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4080 Fragments arranged by size: 4080 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2900 3860 4219 4378 518 Size: 648 1734 960 359 159 2042 Fragments arranged by size: 2042 1734 960 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2112 2684 3318 3995 5143 5388 1606 Size: 506 572 634 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 634 572 506 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4393 Fragments arranged by size: 4393 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 2264 2572 3260 4805 1132 Size: 97 1035 308 688 1545 2229 Fragments arranged by size: 2229 1545 1035 688 308 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 2485 2984 1108 Size: 1377 499 4026 Fragments arranged by size: 4026 1377 499 PfoI T'CCnGG_A Cuts at: 2734 2734 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2489 3013 2489 Size: 524 5378 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PstI C_TGCA'G Cuts at: 830 3254 830 Size: 2424 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2684 2684 Size: 5902 SacII CC_GC'GG Cuts at: 2113 2113 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2060 Size: 5902 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 2564 4150 5862 2564 Size: 1586 1712 2604 Fragments arranged by size: 2604 1712 1586 SfcI C'TryA_G Cuts at: 826 1045 3250 4385 5066 5257 826 Size: 219 2205 1135 681 191 1471 Fragments arranged by size: 2205 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 2383 2449 2718 4006 4907 Size: 197 926 189 251 66 269 1288 901 Cuts at: 4907 5169 820 Size: 262 1553 Fragments arranged by size: 1553 1288 926 901 269 262 251 197 189 66 SnaBI TAC'GTA Cuts at: 493 3199 3693 493 Size: 2706 494 2702 Fragments arranged by size: 2706 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 3260 1132 Size: 2128 3774 SspI AAT'ATT Cuts at: 5 52 2576 3826 5 Size: 47 2524 1250 2081 Fragments arranged by size: 2524 2081 1250 47 StuI AGG'CCT Cuts at: 1517 2455 2484 1517 Size: 938 29 4935 Fragments arranged by size: 4935 938 29 StyI C'CwwG_G Cuts at: 513 1065 2022 2319 2479 3341 513 Size: 552 957 297 160 862 3074 Fragments arranged by size: 3074 957 862 552 297 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 2508 2928 2971 3929 3946 4099 Size: 1255 471 457 420 43 958 17 153 Cuts at: 4099 4284 4729 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 958 471 457 445 420 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2562 3088 4148 Size: 274 80 33 51 1443 585 526 1060 Cuts at: 4148 5860 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1060 585 526 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5862 5894 5862 Size: 32 5870 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 2412 4031 2412 Size: 1619 4283 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI