Restriction map for
pFN21ASDA0025
(Linear) (Six-base) MAPSORT of: KIAA0025.seq Check: 6876 from: 1 to: 1173
KIAA0025.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 636 1173
Size: 636 537
AccI GT'mk_AC
Cuts at: 0 131 1173
Size: 131 1042
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 487 1052 1173
Size: 455 32 565 121
Fragments arranged by size:
565 455 121 32
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 545 1173
Size: 545 628
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 1173
Size: 388 32 753
Fragments arranged by size:
753 388 32
AlwNI CAG_nnn'CTG
Cuts at: 0 565 1173
Size: 565 608
ApaLI G'TGCA_C
Cuts at: 0 599 1173
Size: 599 574
ApoI r'AATT_y
Cuts at: 0 726 1173
Size: 726 447
AvaI C'yCGr_G
Cuts at: 0 19 136 1173
Size: 19 117 1037
Fragments arranged by size:
1037 117 19
BanII G_rGCy'C
Cuts at: 0 26 49 1173
Size: 26 23 1124
Fragments arranged by size:
1124 26 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 1040 1110 1173
Size: 1040 70 63
Fragments arranged by size:
1040 70 63
BciVI GTATCCnnnnn_n'
Cuts at: 0 356 847 1173
Size: 356 491 326
Fragments arranged by size:
491 356 326
BglI GCCn_nnn'nGGC
Cuts at: 0 301 448 1173
Size: 301 147 725
Fragments arranged by size:
725 301 147
Bme1580I G_kGCm'C
Cuts at: 0 603 1173
Size: 603 570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 375 1173
Size: 375 798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 617 734 1037 1173
Size: 114 503 117 303 136
Fragments arranged by size:
503 303 136 117 114
Bpu10I CC'TnA_GC
Cuts at: 0 121 859 1173
Size: 121 738 314
Fragments arranged by size:
738 314 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 345 411 680 1173
Size: 94 251 66 269 493
Fragments arranged by size:
493 269 251 94 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 4 624 1173
Size: 4 620 549
Fragments arranged by size:
620 549 4
BsaWI w'CCGG_w
Cuts at: 0 478 1173
Size: 478 695
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 835 865 1173
Size: 835 30 308
Fragments arranged by size:
835 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 683 843 959 1173
Size: 331 352 160 116 214
Fragments arranged by size:
352 331 214 160 116
BseYI C'CCAG_C
Cuts at: 0 443 1158 1173
Size: 443 715 15
Fragments arranged by size:
715 443 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 1173
Size: 608 565
BsiEI CG_ry'CG
Cuts at: 0 87 1173
Size: 87 1086
BsiHKAI G_wGCw'C
Cuts at: 0 603 1105 1173
Size: 603 502 68
Fragments arranged by size:
603 502 68
BsmI GAATG_Cn'
Cuts at: 0 451 690 744 1173
Size: 451 239 54 429
Fragments arranged by size:
451 429 239 54
Bsp1286I G_dGCh'C
Cuts at: 0 26 49 603 1105 1173
Size: 26 23 554 502 68
Fragments arranged by size:
554 502 68 26 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 636 911 1173
Size: 636 275 262
Fragments arranged by size:
636 275 262
BsrDI GCAATG_nn'
Cuts at: 0 264 1173
Size: 264 909
BsrFI r'CCGG_y
Cuts at: 0 939 1173
Size: 939 234
BstAPI GCAn_nnn'nTGC
Cuts at: 0 243 565 1173
Size: 243 322 608
Fragments arranged by size:
608 322 243
Bsu36I CC'TnA_GG
Cuts at: 0 350 1173
Size: 350 823
BtgI C'CryG_G
Cuts at: 0 87 1173
Size: 87 1086
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1149 1173
Size: 1149 24
BtsI GCAGTG_nn'
Cuts at: 0 563 1173
Size: 563 610
DraIII CAC_nnn'GTG
Cuts at: 0 598 748 1173
Size: 598 150 425
Fragments arranged by size:
598 425 150
EarI CTCTTCn'nnn_
Cuts at: 0 37 259 1145 1173
Size: 37 222 886 28
Fragments arranged by size:
886 222 37 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 455 487 617 734 1037 1052 1173
Size: 114 341 32 130 117 303 15 121
Fragments arranged by size:
341 303 130 121 117 114 32 15
EcoO109I rG'GnC_Cy
Cuts at: 0 466 990 1153 1173
Size: 466 524 163 20
Fragments arranged by size:
524 466 163 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1146 1173
Size: 1146 27
HaeII r_GCGC'y
Cuts at: 0 60 1173
Size: 60 1113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 773 1173
Size: 170 603 400
Fragments arranged by size:
603 400 170
MslI CAynn'nnrTG
Cuts at: 0 877 1173
Size: 877 296
MspA1I CmG'CkG
Cuts at: 0 89 661 1173
Size: 89 572 512
Fragments arranged by size:
572 512 89
NspI r_CATG'y
Cuts at: 0 241 549 1173
Size: 241 308 624
Fragments arranged by size:
624 308 241
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 961 1173
Size: 462 499 212
Fragments arranged by size:
499 462 212
PfoI T'CCnGG_A
Cuts at: 0 711 1173
Size: 711 462
PpuMI rG'GwC_Cy
Cuts at: 0 466 990 1173
Size: 466 524 183
Fragments arranged by size:
524 466 183
PvuII CAG'CTG
Cuts at: 0 661 1173
Size: 661 512
SacII CC_GC'GG
Cuts at: 0 90 1173
Size: 90 1083
SapI GCTCTTCn'nnn_
Cuts at: 0 37 1173
Size: 37 1136
ScaI AGT'ACT
Cuts at: 0 541 1173
Size: 541 632
SmlI C'TyrA_G
Cuts at: 0 109 360 426 695 1173
Size: 109 251 66 269 478
Fragments arranged by size:
478 269 251 109 66
SspI AAT'ATT
Cuts at: 0 553 1173
Size: 553 620
StuI AGG'CCT
Cuts at: 0 432 461 1173
Size: 432 29 712
Fragments arranged by size:
712 432 29
StyI C'CwwG_G
Cuts at: 0 296 456 1173
Size: 296 160 717
Fragments arranged by size:
717 296 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 28 485 905 948 1173
Size: 28 457 420 43 225
Fragments arranged by size:
457 420 225 43 28
TatI w'GTAC_w
Cuts at: 0 539 1065 1173
Size: 539 526 108
Fragments arranged by size:
539 526 108
XmnI GAAnn'nnTTC
Cuts at: 0 389 1173
Size: 389 784
Enzymes that do cut:
AarI AccI AcuI AlfI AloI AlwNI ApaLI ApoI
AvaI BanII BbsI BciVI BglI Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI
Bsu36I BtgI BtgZI BtsI DraIII EarI Eco57MI EcoO109I
FalI HaeII MmeI MslI MspA1I NspI PflMI PfoI
PpuMI PvuII SacII SapI ScaI SmlI SspI StuI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BsaAI BsaBI BsaHI BsiWI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I ClaI CspCI DraI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI