Restriction map for
pFN21ASDA0024
(Linear) (Six-base) MAPSORT of: KIAA0024.seq Check: 1743 from: 1 to: 1419
KIAA0024.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 1419
Size: 1337 82
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1296 1328 1419
Size: 1296 32 91
Fragments arranged by size:
1296 91 32
ApoI r'AATT_y
Cuts at: 0 363 968 1108 1419
Size: 363 605 140 311
Fragments arranged by size:
605 363 311 140
BanI G'GyrC_C
Cuts at: 0 1264 1419
Size: 1264 155
BanII G_rGCy'C
Cuts at: 0 602 1419
Size: 602 817
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 1325 1419
Size: 203 1122 94
Fragments arranged by size:
1122 203 94
BclI T'GATC_A
Cuts at: 0 68 464 881 1419
Size: 68 396 417 538
Fragments arranged by size:
538 417 396 68
BfrBI ATG'CAT
Cuts at: 0 57 612 1419
Size: 57 555 807
Fragments arranged by size:
807 555 57
BglI GCCn_nnn'nGGC
Cuts at: 0 535 1419
Size: 535 884
BglII A'GATC_T
Cuts at: 0 1119 1280 1419
Size: 1119 161 139
Fragments arranged by size:
1119 161 139
BlpI GC'TnA_GC
Cuts at: 0 136 1419
Size: 136 1283
BmrI ACTGGGnnnn_n'
Cuts at: 0 747 1419
Size: 747 672
BmtI G_CTAG'C
Cuts at: 0 812 1419
Size: 812 607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 235 1419
Size: 235 1184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 884 1108 1419
Size: 228 656 224 311
Fragments arranged by size:
656 311 228 224
Bpu10I CC'TnA_GC
Cuts at: 0 28 1419
Size: 28 1391
BsaI GGTCTCn'nnnn_
Cuts at: 0 565 1419
Size: 565 854
BsaBI GATnn'nnATC
Cuts at: 0 404 1419
Size: 404 1015
BsaHI Gr'CG_yC
Cuts at: 0 4 1419
Size: 4 1415
BsaWI w'CCGG_w
Cuts at: 0 62 1419
Size: 62 1357
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 607 1419
Size: 607 812
BseYI C'CCAG_C
Cuts at: 0 524 693 1061 1419
Size: 524 169 368 358
Fragments arranged by size:
524 368 358 169
BsiEI CG_ry'CG
Cuts at: 0 118 1419
Size: 118 1301
BsiHKAI G_wGCw'C
Cuts at: 0 329 602 1419
Size: 329 273 817
Fragments arranged by size:
817 329 273
BsmI GAATG_Cn'
Cuts at: 0 1380 1419
Size: 1380 39
Bsp1286I G_dGCh'C
Cuts at: 0 329 602 1419
Size: 329 273 817
Fragments arranged by size:
817 329 273
BspEI T'CCGG_A
Cuts at: 0 62 1419
Size: 62 1357
BspHI T'CATG_A
Cuts at: 0 878 1419
Size: 878 541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 363 1419
Size: 363 1056
BsrDI GCAATG_nn'
Cuts at: 0 681 1419
Size: 681 738
BsrFI r'CCGG_y
Cuts at: 0 113 710 1419
Size: 113 597 709
Fragments arranged by size:
709 597 113
BstEII G'GTnAC_C
Cuts at: 0 1228 1419
Size: 1228 191
BstXI CCAn_nnnn'nTGG
Cuts at: 0 299 959 1419
Size: 299 660 460
Fragments arranged by size:
660 460 299
BstYI r'GATC_y
Cuts at: 0 399 1119 1280 1419
Size: 399 720 161 139
Fragments arranged by size:
720 399 161 139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 663 698 1419
Size: 663 35 721
Fragments arranged by size:
721 663 35
DraIII CAC_nnn'GTG
Cuts at: 0 1037 1419
Size: 1037 382
EaeI y'GGCC_r
Cuts at: 0 115 1419
Size: 115 1304
EagI C'GGCC_G
Cuts at: 0 115 1419
Size: 115 1304
EarI CTCTTCn'nnn_
Cuts at: 0 361 607 1129 1419
Size: 361 246 522 290
Fragments arranged by size:
522 361 290 246
EcoICRI GAG'CTC
Cuts at: 0 600 1419
Size: 600 819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 884 1108 1337 1419
Size: 228 656 224 229 82
Fragments arranged by size:
656 229 228 224 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 174 474 1419
Size: 31 143 300 945
Fragments arranged by size:
945 300 143 31
EcoRI G'AATT_C
Cuts at: 0 968 1419
Size: 968 451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 945 1376 1419
Size: 945 431 43
Fragments arranged by size:
945 431 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 826 858 1106 1111 1138 1143 1359 1391
Size: 826 32 248 5 27 5 216 32
Cuts at: 1391 1419
Size: 28
Fragments arranged by size:
826 248 216 32 32 28 27 5
5
HindIII A'AGCT_T
Cuts at: 0 744 1419
Size: 744 675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1389 1419
Size: 1389 30
MslI CAynn'nnrTG
Cuts at: 0 250 1419
Size: 250 1169
MspA1I CmG'CkG
Cuts at: 0 894 1419
Size: 894 525
NheI G'CTAG_C
Cuts at: 0 808 1419
Size: 808 611
NsiI A_TGCA'T
Cuts at: 0 59 614 1419
Size: 59 555 805
Fragments arranged by size:
805 555 59
NspI r_CATG'y
Cuts at: 0 1339 1419
Size: 1339 80
PfoI T'CCnGG_A
Cuts at: 0 1085 1363 1419
Size: 1085 278 56
Fragments arranged by size:
1085 278 56
PstI C_TGCA'G
Cuts at: 0 797 1419
Size: 797 622
PvuII CAG'CTG
Cuts at: 0 894 1419
Size: 894 525
SacI G_AGCT'C
Cuts at: 0 602 1419
Size: 602 817
SapI GCTCTTCn'nnn_
Cuts at: 0 607 1419
Size: 607 812
ScaI AGT'ACT
Cuts at: 0 1012 1419
Size: 1012 407
SfcI C'TryA_G
Cuts at: 0 793 1269 1419
Size: 793 476 150
Fragments arranged by size:
793 476 150
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 535 1419
Size: 535 884
SphI G_CATG'C
Cuts at: 0 1339 1419
Size: 1339 80
StuI AGG'CCT
Cuts at: 0 538 1419
Size: 538 881
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1053 1344 1419
Size: 1053 291 75
Fragments arranged by size:
1053 291 75
TatI w'GTAC_w
Cuts at: 0 161 1010 1419
Size: 161 849 409
Fragments arranged by size:
849 409 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 1257 1289 1419
Size: 308 32 917 32 130
Fragments arranged by size:
917 308 130 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1283 1419
Size: 1283 136
XmnI GAAnn'nnTTC
Cuts at: 0 910 1419
Size: 910 509
Enzymes that do cut:
AcuI AloI ApoI BanI BanII BbsI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI BpmI Bpu10I
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstEII
BstXI BstYI CspCI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoRI FalI Hin4I HindIII MmeI MslI
MspA1I NheI NsiI NspI PfoI PstI PvuII SacI
SapI ScaI SfcI SfiI SphI StuI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI Bme1580I BmgBI BpuEI BsaAI BsaXI BsgI
BsiWI BsmBI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EciI EcoO109I EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StyI SwaI Tth111I XbaI XhoI XmaI
ZraI