Restriction map for
pFN21ASDA0023
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# NO CUT ENZYMES ( 85 enzymes) #
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AccIII AflIII AgeI AhdI ApaI
AscI AspEI AspI AviII AvrII
BbrPI BglII BlnI BsaAI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NotI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 1099 GTAGAC GT^[AC][GT]AC
AccII 6214 CGCG CG^CG
AclI 3309 AACGTT AA^CGTT
AflII 3451 CTTAAG C^TTAAG
Alw44I 3200 GTGCAC G^TGCAC
Aor51HI 60 AGCGCT AGC^GCT
ApaLI 3200 GTGCAC G^TGCAC
AspHI 3200 GTGCAC G[AT]GC[AT]^C
BbeI 2411 GGCGCC GGCGC^C
BbvCI 5402 CCTCAGC CCTCAGC(-5/-2)
BciVI 980 GTATCC GTATCC(6/5)
BclI 999 TGATCA T^GATCA
BfrI 3451 CTTAAG C^TTAAG
BsaWI 3478 TCCGGT [AT]^CCGG[AT]
BsiHKAI 3200 GTGCAC G[AT]GC[AT]^C
BstUI 6214 CGCG CG^CG
BstXI 5805 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 60 AGCGCT AGC^GCT
EcoNI 3754 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 999 TGATCA T^GATCA
KasI 2411 GGCGCC G^GCGCC
MvnI 6214 CGCG CG^CG
NarI 2411 GGCGCC GG^CGCC
NheI 3136 GCTAGC G^CTAGC
NruI 6213 TCGCGA TCG^CGA
PaeR7I 114 CTCGAG C^TCGAG
PshAI 4716 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3309 AACGTT AA^CGTT
SfoI 2411 GGCGCC GGC^GCC
StuI 5120 AGGCCT AGG^CCT
XhoI 114 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 2967 GACGTC GACGT^C
4029 GACGTC
AseI 1006 ATTAAT AT^TAAT
1210 ATTAAT
AsnI 1006 ATTAAT AT^TAAT
1210 ATTAAT
Asp700I 3932 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4555 GAACCTGTTC
AvaI 28 CCCGAG C^[CT]CG[GA]G
114 CTCGAG
BcgI 9 CGAGATGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5963 GCAAAGTGTTCG
BsaBI 2059 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2476 GATCAGCATC
BsoBI 28 CCCGAG C^[CT]CG[GA]G
114 CTCGAG
BsrBI 6095 CCGCTC CCGCTC(-3/-3)
31 GAGCGG
DrdI 2235 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4456 GACAGTGAGGTC
HaeII 60 AGCGCT [GA]GCGC^[CT]
2411 GGCGCC
HgaI 3694 GACGC GACGC(5/10)
1606 GCGTC
HincII 1753 GTCAAC GT[CT]^[GA]AC
3399 GTTGAC
HindII 1753 GTCAAC GT[CT]^[GA]AC
3399 GTTGAC
MamI 2059 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2476 GATCAGCATC
NcoI 488 CCATGG C^CATGG
1085 CCATGG
PshBI 1006 ATTAAT AT^TAAT
1210 ATTAAT
XbaI 2460 TCTAGA T^CTAGA
4488 TCTAGA
XcmI 6166 CCATTAAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6250 CCAACAGACTTCTGG
XmnI 3932 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4555 GAACCTGTTC
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
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Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp718I AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmlI
SpeI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII