(Circular) (Six-base) MAPSORT of: pFN21ASDA0023.seq Check: 2885 from: 1 to: 11008 pFN21ASDA0023 11008 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3418 4542 6718 3418 Size: 1124 2176 7708 Fragments arranged by size: 7708 2176 1124 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4994 6056 8818 278 Size: 53 83 186 936 3458 1062 2762 2468 Fragments arranged by size: 3458 2762 2468 1062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3123 8351 1937 Size: 1186 5228 4594 Fragments arranged by size: 5228 4594 1186 Acc65I G'GTAC_C Cuts at: 2771 6866 7100 7421 8329 2771 Size: 4095 234 321 908 5450 Fragments arranged by size: 5450 4095 908 321 234 AclI AA'CG_TT Cuts at: 1563 5333 9135 9508 1563 Size: 3770 3802 373 3063 Fragments arranged by size: 3802 3770 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4025 4406 4619 5127 5514 7177 7461 7600 Size: 2393 381 213 508 387 1663 284 139 Cuts at: 7600 8084 9071 10455 10723 1632 Size: 484 987 1384 268 1917 Fragments arranged by size: 2393 1917 1663 1384 987 508 484 387 381 284 268 213 139 AfeI AGC'GCT Cuts at: 2085 2085 Size: 11008 AflII C'TTAA_G Cuts at: 820 1017 5474 820 Size: 197 4457 6354 Fragments arranged by size: 6354 4457 197 AflIII A'CryG_T Cuts at: 1203 9907 10884 1203 Size: 8704 977 1327 Fragments arranged by size: 8704 1327 977 AgeI A'CCGG_T Cuts at: 9839 9839 Size: 11008 AhdI GACnn_n'nnGTC Cuts at: 9737 9737 Size: 11008 AleI CACnn'nnGTG Cuts at: 1166 3704 6974 1166 Size: 2538 3270 5200 Fragments arranged by size: 5200 3270 2538 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3722 5280 8093 1184 Size: 2538 1558 2813 4099 Fragments arranged by size: 4099 2813 2538 1558 AlwNI CAG_nnn'CTG Cuts at: 2981 3361 3386 3566 4091 4507 4969 5435 5621 Size: 380 25 180 525 416 462 466 186 Cuts at: 5621 6755 6776 6956 7052 8751 10323 2981 Size: 1134 21 180 96 1699 1572 3666 Fragments arranged by size: 3666 1699 1572 1134 525 466 462 416 380 186 180 180 96 25 21 ApaLI G'TGCA_C Cuts at: 5223 9065 10221 5223 Size: 3842 1156 6010 Fragments arranged by size: 6010 3842 1156 ApoI r'AATT_y Cuts at: 1493 2297 4428 4454 5467 8317 8543 8695 10804 Size: 804 2131 26 1013 2850 226 152 2109 Cuts at: 10804 1493 Size: 1697 Fragments arranged by size: 2850 2131 2109 1697 1013 804 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 3030 3234 9562 160 Size: 872 1998 204 6328 1606 Fragments arranged by size: 6328 1998 1606 872 204 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11008 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2137 8333 1870 Size: 73 108 86 6196 4545 Fragments arranged by size: 6196 4545 108 86 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2763 2796 2763 Size: 33 10975 BamHI G'GATC_C Cuts at: 4399 6218 7691 7910 8338 8705 4399 Size: 1819 1473 219 428 367 6702 Fragments arranged by size: 6702 1819 1473 428 367 219 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2771 4434 4530 5302 5848 Size: 325 217 627 984 1663 96 772 546 Cuts at: 5848 6866 7100 7277 7421 7930 8329 9784 618 Size: 1018 234 177 144 509 399 1455 1842 Fragments arranged by size: 1842 1663 1455 1018 984 772 627 546 509 399 325 234 217 177 144 96 BanII G_rGCy'C Cuts at: 1391 2627 2901 3457 3519 6117 7976 8327 1391 Size: 1236 274 556 62 2598 1859 351 4072 Fragments arranged by size: 4072 2598 1859 1236 556 351 274 62 BbeI G_GCGC'C Cuts at: 4438 4438 Size: 11008 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2167 2773 4625 4859 4932 5649 7317 Size: 971 268 606 1852 234 73 717 1668 Cuts at: 7317 928 Size: 4619 Fragments arranged by size: 4619 1852 1668 971 717 606 268 234 73 BbvCI CC'TCA_GC Cuts at: 7426 7426 Size: 11008 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2021 2055 7975 8009 9199 9233 1903 Size: 34 84 34 5920 34 1190 34 3678 Fragments arranged by size: 5920 3678 1190 84 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3014 8900 10110 3014 Size: 5886 1210 3912 Fragments arranged by size: 5886 3912 1210 BclI T'GATC_A Cuts at: 1548 3022 1548 Size: 1474 9534 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5258 5300 5485 Size: 107 122 71 631 605 3586 42 185 Cuts at: 5485 7971 9619 136 Size: 2486 1648 1525 Fragments arranged by size: 3586 2486 1648 1525 631 605 185 122 107 71 42 BglII A'GATC_T Cuts at: 11003 11003 Size: 11008 BlpI GC'TnA_GC Cuts at: 4416 6897 6922 8425 4416 Size: 2481 25 1503 6999 Fragments arranged by size: 6999 2481 1503 25 Bme1580I G_kGCm'C Cuts at: 1790 3969 4187 5227 6066 6312 8006 9069 10225 Size: 2179 218 1040 839 246 1694 1063 1156 Cuts at: 10225 1790 Size: 2573 Fragments arranged by size: 2573 2179 1694 1156 1063 1040 839 246 218 BmgBI CAC'GTC Cuts at: 1318 6822 1318 Size: 5504 5504 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 3956 4957 Size: 416 53 83 399 24 2102 437 1001 Cuts at: 4957 5061 5411 5423 7632 8739 9697 442 Size: 104 350 12 2209 1107 958 1753 Fragments arranged by size: 2209 2102 1753 1107 1001 958 437 416 399 350 104 83 53 24 12 BmtI G_CTAG'C Cuts at: 1056 5163 1056 Size: 4107 6901 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4431 8070 4431 Size: 3639 7369 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2641 2811 3071 3165 3987 4863 4991 5961 Size: 1402 170 260 94 822 876 128 970 Cuts at: 5961 6796 7285 7827 7863 9668 1239 Size: 835 489 542 36 1805 2579 Fragments arranged by size: 2579 1805 1402 970 876 835 822 542 489 260 170 128 94 36 Bpu10I CC'TnA_GC Cuts at: 3945 7075 7426 3945 Size: 3130 351 7527 Fragments arranged by size: 7527 3130 351 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2341 3330 4328 5160 5334 6748 7270 9133 9998 Size: 989 998 832 174 1414 522 1863 865 Cuts at: 9998 10296 2341 Size: 298 3053 Fragments arranged by size: 3053 1863 1414 998 989 865 832 522 298 174 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4250 5463 6493 9671 882 Size: 617 2751 1213 1030 3178 2219 Fragments arranged by size: 3178 2751 2219 1213 1030 617 BsaAI yAC'GTr Cuts at: 493 1204 8305 8799 493 Size: 711 7101 494 2702 Fragments arranged by size: 7101 2702 711 494 BsaBI GATnn'nnATC Cuts at: 4086 4503 11002 4086 Size: 417 6499 4092 Fragments arranged by size: 6499 4092 417 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4435 4991 6053 8815 Size: 53 83 186 936 2902 556 1062 2762 Cuts at: 8815 9197 9832 275 Size: 382 635 1451 Fragments arranged by size: 2902 2762 1451 1062 936 635 556 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5501 9441 9839 10113 10260 10671 5501 Size: 3940 398 274 147 411 5838 Fragments arranged by size: 5838 3940 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2460 2490 3105 3135 3157 Size: 30 165 30 1057 30 615 30 22 Cuts at: 3157 3187 6509 6539 7395 7425 7803 7833 7974 Size: 30 3322 30 856 30 378 30 141 Cuts at: 7974 8004 1178 Size: 30 4182 Fragments arranged by size: 4182 3322 1057 856 615 378 165 141 30 30 30 30 30 30 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3331 4127 4208 6530 6537 7824 8115 1186 Size: 2145 796 81 2322 7 1287 291 4079 Fragments arranged by size: 4079 2322 2145 1287 796 291 81 7 BseYI C'CCAG_C Cuts at: 5908 6039 6531 6591 6664 7090 7951 10211 10707 Size: 131 492 60 73 426 861 2260 496 Cuts at: 10707 5908 Size: 6209 Fragments arranged by size: 6209 2260 861 496 492 426 131 73 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2899 5384 5658 6234 6789 1163 Size: 573 1163 2485 274 576 555 5382 Fragments arranged by size: 5382 2485 1163 576 573 555 274 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8458 9219 10247 1540 Size: 275 186 6457 761 1028 2301 Fragments arranged by size: 6457 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5227 9069 9154 10225 5227 Size: 3842 85 1071 6010 Fragments arranged by size: 6010 3842 1071 85 BsmI GAATG_Cn' Cuts at: 4704 5720 7937 8252 8519 8612 4704 Size: 1016 2217 315 267 93 7100 Fragments arranged by size: 7100 2217 1016 315 267 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2022 2829 3636 2022 Size: 807 807 9394 Fragments arranged by size: 9394 807 807 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2627 2901 3457 3519 3969 4187 5227 Size: 399 837 274 556 62 450 218 1040 Cuts at: 5227 6066 6117 6312 7976 8006 8327 9069 9154 Size: 839 51 195 1664 30 321 742 85 Cuts at: 9154 10225 1391 Size: 1071 2174 Fragments arranged by size: 2174 1664 1071 1040 839 837 742 556 450 399 321 274 218 195 85 62 51 30 BspEI T'CCGG_A Cuts at: 10671 10671 Size: 11008 BspHI T'CATG_A Cuts at: 8897 10627 8897 Size: 1730 9278 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3418 4542 5830 6718 8363 844 Size: 2574 1124 1288 888 1645 3489 Fragments arranged by size: 3489 2574 1645 1288 1124 888 BsrBI CCG'CTC Cuts at: 1394 1823 2056 8120 8895 1394 Size: 429 233 6064 775 3507 Fragments arranged by size: 6064 3507 775 429 233 BsrDI GCAATG_nn' Cuts at: 65 1248 3420 3741 4747 9503 9677 65 Size: 1183 2172 321 1006 4756 174 1396 Fragments arranged by size: 4756 2172 1396 1183 1006 321 174 BsrFI r'CCGG_y Cuts at: 1700 1810 9652 9839 1700 Size: 110 7842 187 2869 Fragments arranged by size: 7842 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 11008 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11008 BssSI C'ACGA_G Cuts at: 9068 10080 9068 Size: 1012 9996 BstAPI GCAn_nnn'nTGC Cuts at: 794 2981 3386 5093 6650 794 Size: 2187 405 1707 1557 5152 Fragments arranged by size: 5152 2187 1707 1557 405 BstBI TT'CG_AA Cuts at: 2007 9812 2007 Size: 7805 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 11008 BstXI CCAn_nnnn'nTGG Cuts at: 7835 7835 Size: 11008 BstYI r'GATC_y Cuts at: 1972 4087 4399 4868 6218 7691 7910 8147 8338 Size: 2115 312 469 1350 1473 219 237 191 Cuts at: 8338 8705 9090 9107 10559 10645 10657 11003 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2115 1977 1473 1452 1350 469 385 367 346 312 237 219 191 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2115 5782 6067 2115 Size: 3667 285 7056 Fragments arranged by size: 7056 3667 285 BtgI C'CryG_G Cuts at: 513 1065 2022 2511 2595 3108 5040 6672 513 Size: 552 957 489 84 513 1932 1632 4849 Fragments arranged by size: 4849 1932 1632 957 552 513 489 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 10269 Fragments arranged by size: 10269 683 56 BtsI GCAGTG_nn' Cuts at: 2652 4872 5019 5667 6615 6954 7177 8004 8524 Size: 2220 147 648 948 339 223 827 520 Cuts at: 8524 9318 9338 2652 Size: 794 20 4322 Fragments arranged by size: 4322 2220 948 827 794 648 520 339 223 147 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11008 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3120 3155 6808 6843 7843 7878 529 Size: 35 2556 35 3653 35 1000 35 3659 Fragments arranged by size: 3659 3653 2556 1000 35 35 35 35 DraI TTT'AAA Cuts at: 8312 8312 Size: 11008 DraIII CAC_nnn'GTG Cuts at: 4624 5129 5419 4624 Size: 505 290 10213 Fragments arranged by size: 10213 505 290 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4264 6485 10015 809 Size: 507 2948 2221 3530 1802 Fragments arranged by size: 3530 2948 2221 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4842 5292 5977 Size: 54 1425 50 275 18 3012 450 685 Cuts at: 5977 6780 7354 7546 7674 8040 8155 8455 9344 Size: 803 574 192 128 366 115 300 889 Cuts at: 9344 10973 8 Size: 1629 43 Fragments arranged by size: 3012 1629 1425 889 803 685 574 450 366 300 275 192 128 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8455 1537 Size: 275 6643 4090 Fragments arranged by size: 6643 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2592 3174 3832 4137 4601 6435 7567 7594 Size: 1239 582 658 305 464 1834 1132 27 Cuts at: 7594 8938 1353 Size: 1344 3423 Fragments arranged by size: 3423 1834 1344 1239 1132 658 582 464 305 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4634 9595 9969 10115 387 Size: 1064 138 3045 4961 374 146 1280 Fragments arranged by size: 4961 3045 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2641 2811 3071 3165 3987 4025 4406 Size: 393 1009 170 260 94 822 38 381 Cuts at: 4406 4619 4863 4991 5127 5514 5961 6796 7177 Size: 213 244 128 136 387 447 835 381 Cuts at: 7177 7285 7461 7600 7827 7863 8084 9071 9668 Size: 108 176 139 227 36 221 987 597 Cuts at: 9668 10455 10723 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1009 987 835 822 787 597 447 393 387 381 381 268 260 244 227 221 213 176 170 139 136 128 108 94 38 36 EcoNI CCTnn'n_nnAGG Cuts at: 5781 5781 Size: 11008 EcoO109I rG'GnC_Cy Cuts at: 2811 3563 5023 6679 6773 8034 8293 2811 Size: 752 1460 1656 94 1261 259 5526 Fragments arranged by size: 5526 1656 1460 1261 752 259 94 EcoRI G'AATT_C Cuts at: 8317 8695 8317 Size: 378 10630 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3763 3797 4436 5763 3763 Size: 34 639 1327 9008 Fragments arranged by size: 9008 1327 639 34 FspI TGC'GCA Cuts at: 9514 9514 Size: 11008 HaeII r_GCGC'y Cuts at: 1678 2087 4438 10155 1678 Size: 409 2351 5717 2531 Fragments arranged by size: 5717 2531 2351 409 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2914 2946 3157 3189 3985 Size: 32 135 32 1342 32 211 32 796 Cuts at: 3985 4017 4600 4632 5281 5313 5708 5740 6509 Size: 32 583 32 649 32 395 32 769 Cuts at: 6509 6541 7395 7427 8132 8164 9649 9681 9723 Size: 32 854 32 705 32 1485 32 42 Cuts at: 9723 9755 1373 Size: 32 2626 Fragments arranged by size: 2626 1485 1342 854 796 769 705 649 583 395 211 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3778 5424 8352 8598 669 Size: 988 281 1840 1646 2928 246 3079 Fragments arranged by size: 3079 2928 1840 1646 988 281 246 HindIII A'AGCT_T Cuts at: 748 2450 5526 7473 748 Size: 1702 3076 1947 4283 Fragments arranged by size: 4283 3076 1947 1702 HpaI GTT'AAC Cuts at: 8598 8598 Size: 11008 KasI G'GCGC_C Cuts at: 4434 4434 Size: 11008 KpnI G_GTAC'C Cuts at: 2775 6870 7104 7425 8333 2775 Size: 4095 234 321 908 5450 Fragments arranged by size: 5450 4095 908 321 234 MfeI C'AATT_G Cuts at: 8607 8607 Size: 11008 MluI A'CGCG_T Cuts at: 10884 10884 Size: 11008 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 3564 4119 6377 8081 10122 10306 1305 Size: 876 1383 555 2258 1704 2041 184 2007 Fragments arranged by size: 2258 2041 2007 1704 1383 876 555 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4844 5294 5979 7356 7548 Size: 54 1425 343 3012 450 685 1377 192 Cuts at: 7548 8042 8157 10 Size: 494 115 2861 Fragments arranged by size: 3012 2861 1425 1377 685 494 450 343 192 115 54 MslI CAynn'nnrTG Cuts at: 518 1166 3704 4446 6974 8966 9325 9484 518 Size: 648 2538 742 2528 1992 359 159 2042 Fragments arranged by size: 2538 2528 2042 1992 742 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2641 2913 3377 3650 3845 4007 5264 5309 Size: 1035 272 464 273 195 162 1257 45 Cuts at: 5309 5555 6338 6425 6755 7052 7430 8012 8424 Size: 246 783 87 330 297 378 582 412 Cuts at: 8424 9101 10249 10494 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1257 1148 1035 783 677 582 464 412 378 330 297 273 272 246 245 195 162 87 45 NarI GG'CG_CC Cuts at: 4435 4435 Size: 11008 NcoI C'CATG_G Cuts at: 513 1065 2022 2511 3108 513 Size: 552 957 489 597 8413 Fragments arranged by size: 8413 957 597 552 489 NdeI CA'TA_TG Cuts at: 387 387 Size: 11008 NheI G'CTAG_C Cuts at: 1052 5159 1052 Size: 4107 6901 NotI GC'GGCC_GC Cuts at: 8455 8455 Size: 11008 NruI TCG'CGA Cuts at: 8238 8238 Size: 11008 NspI r_CATG'y Cuts at: 1132 1229 8366 9911 1132 Size: 97 7137 1545 2229 Fragments arranged by size: 7137 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 7112 7916 1348 Size: 5764 804 4440 Fragments arranged by size: 5764 4440 804 PciI A'CATG_T Cuts at: 9907 9907 Size: 11008 PflMI CCAn_nnn'nTGG Cuts at: 1108 2314 3566 4969 7097 7886 1108 Size: 1206 1252 1403 2128 789 4230 Fragments arranged by size: 4230 2128 1403 1252 1206 789 PmeI GTTT'AAAC Cuts at: 8312 8312 Size: 11008 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9077 9109 10590 10622 9077 Size: 32 1481 32 9463 Fragments arranged by size: 9463 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2811 6679 8034 8293 2811 Size: 3868 1355 259 5526 Fragments arranged by size: 5526 3868 1355 259 PshAI GACnn'nnGTC Cuts at: 1613 6743 1613 Size: 5130 5878 PsiI TTA'TAA Cuts at: 4386 8578 4386 Size: 4192 6816 PspXI vC'TCGA_Gb Cuts at: 2137 2137 Size: 11008 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 7288 7320 7486 7518 7288 Size: 32 166 32 10778 Fragments arranged by size: 10778 166 32 32 PstI C_TGCA'G Cuts at: 830 2806 3376 3382 3628 6601 6622 6655 6850 Size: 1976 570 6 246 2973 21 33 195 Cuts at: 6850 8011 8360 830 Size: 1161 349 3478 Fragments arranged by size: 3478 2973 1976 1161 570 349 246 195 33 21 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11008 PvuII CAG'CTG Cuts at: 2641 2913 3377 3650 3845 5264 5309 5555 6338 Size: 272 464 273 195 1419 45 246 783 Cuts at: 6338 6755 7052 7430 8012 2641 Size: 417 297 378 582 5637 Fragments arranged by size: 5637 1419 783 582 464 417 378 297 273 272 246 195 45 SalI G'TCGA_C Cuts at: 1936 8350 1936 Size: 6414 4594 SanDI GG'GwC_CC Cuts at: 2811 6679 8034 8293 2811 Size: 3868 1355 259 5526 Fragments arranged by size: 5526 3868 1355 259 SbfI CC_TGCA'GG Cuts at: 8360 8360 Size: 11008 ScaI AGT'ACT Cuts at: 3322 6631 7528 9256 10968 3322 Size: 3309 897 1728 1712 3362 Fragments arranged by size: 3362 3309 1728 1712 897 SexAI A'CCwGG_T Cuts at: 4620 5106 5125 4620 Size: 486 19 10503 Fragments arranged by size: 10503 486 19 SfcI C'TryA_G Cuts at: 826 1045 2802 3372 3378 3624 5397 6333 6597 Size: 219 1757 570 6 246 1773 936 264 Cuts at: 6597 6618 6651 6846 8007 8356 9491 10172 10363 Size: 21 33 195 1161 349 1135 681 191 Cuts at: 10363 826 Size: 1471 Fragments arranged by size: 1773 1757 1471 1161 1135 936 681 570 349 264 246 219 195 191 33 21 6 SfoI GGC'GCC Cuts at: 4436 4436 Size: 11008 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11008 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11008 SmaI CCC'GGG Cuts at: 1872 8335 1872 Size: 6463 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2137 2320 3345 4307 5175 5349 Size: 197 926 194 183 1025 962 868 174 Cuts at: 5349 5474 6763 7285 9112 10013 10275 820 Size: 125 1289 522 1827 901 262 1553 Fragments arranged by size: 1827 1553 1289 1025 962 926 901 868 522 262 197 194 183 174 125 SnaBI TAC'GTA Cuts at: 493 8305 8799 493 Size: 7812 494 2702 Fragments arranged by size: 7812 2702 494 SpeI A'CTAG_T Cuts at: 152 6032 6269 6632 152 Size: 5880 237 363 4528 Fragments arranged by size: 5880 4528 363 237 SphI G_CATG'C Cuts at: 1132 8366 1132 Size: 7234 3774 SspI AAT'ATT Cuts at: 5 52 8932 5 Size: 47 8880 2081 Fragments arranged by size: 8880 2081 47 StuI AGG'CCT Cuts at: 1517 7145 1517 Size: 5628 5380 StyI C'CwwG_G Cuts at: 513 1065 2022 2127 2275 2511 3108 7624 7828 Size: 552 957 105 148 236 597 4516 204 Cuts at: 7828 8091 8152 8447 513 Size: 263 61 295 3074 Fragments arranged by size: 4516 3074 957 597 552 295 263 236 204 148 105 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2886 4003 5430 7217 9035 9052 9205 Size: 1255 1306 1117 1427 1787 1818 17 153 Cuts at: 9205 9390 9835 325 Size: 185 445 1498 Fragments arranged by size: 1818 1787 1498 1427 1306 1255 1117 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3320 6629 7526 Size: 274 80 33 51 1443 1343 3309 897 Cuts at: 7526 9254 10966 96 Size: 1728 1712 138 Fragments arranged by size: 3309 1728 1712 1443 1343 897 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2460 2492 2747 2779 4721 4753 4763 4795 5130 Size: 32 255 32 1942 32 10 32 335 Cuts at: 5130 5162 6334 6366 7803 7835 7974 8006 10968 Size: 32 1172 32 1437 32 139 32 2962 Cuts at: 10968 11000 2460 Size: 32 2468 Fragments arranged by size: 2962 2468 1942 1437 1172 335 255 139 32 32 32 32 32 32 32 32 32 10 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11008 XbaI T'CTAG_A Cuts at: 4483 6511 8344 4483 Size: 2028 1833 7147 Fragments arranged by size: 7147 2028 1833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 8196 8280 8196 Size: 84 10924 XhoI C'TCGA_G Cuts at: 1943 2137 1943 Size: 194 10814 XmaI C'CCGG_G Cuts at: 1870 8333 1870 Size: 6463 4545 XmnI GAAnn'nnTTC Cuts at: 5959 6582 9137 5959 Size: 623 2555 7830 Fragments arranged by size: 7830 2555 623 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4992 6054 8816 276 Size: 53 83 186 936 3458 1062 2762 2468 Fragments arranged by size: 3458 2762 2468 1062 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspOMI RsrII SacI SacII SapI SfiI SrfI SwaI