Restriction map for
pFN21ASDA0020
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII HgaI HincII
HindII HpaI HpaII KpnI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 646 GTCTAC GT^[AC][GT]AC
AccII 634 CGCG CG^CG
AcyI 670 GGCGCC G[GA]^CG[CT]C
BbeI 670 GGCGCC GGCGC^C
BbrPI 301 CACGTG CAC^GTG
BbsI 741 GAAGAC GAAGAC(2/6)
BpuAI 741 GAAGAC GAAGAC(2/6)
BsaAI 301 CACGTG [CT]AC^GT[GA]
BsaHI 670 GGCGCC G[GA]^CG[CT]C
BseRI 1396 CTCCTC GAGGAG(10/8)
BsmAI 1139 GAGAC GTCTC(1/5)
BsmBI 1139 GAGACG CGTCTC(1/5)
BsmI 1158 GAATGC GAATGC(1/-1)
BspMI 1058 ACCTGC ACCTGC(4/8)
BssSI 119 CTCGTG CACGAG(-5/-1)
BstUI 634 CGCG CG^CG
CfoI 671 GCGC GCG^C
DraI 292 TTTAAA TTT^AAA
DraII 899 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 397 GAAGAG CTCTTC(1/4)
EcoO109I 899 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 710 CCAAGG C^C[AT][AT]GG
HaeII 670 GGCGCC [GA]GCGC^[CT]
HhaI 671 GCGC GCG^C
Hin1I 670 GGCGCC G[GA]^CG[CT]C
HinP1I 671 GCGC G^CGC
HphI 441 TCACC GGTGA(8/7)
KasI 670 GGCGCC G^GCGCC
Ksp632I 397 GAAGAG CTCTTC(1/4)
MslI 1192 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 634 CGCG CG^CG
NarI 670 GGCGCC GG^CGCC
PflMI 1074 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 301 CACGTG CAC^GTG
PmlI 301 CACGTG CAC^GTG
PpuMI 899 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1235 CTGCAG CTGCA^G
SapI 397 GAAGAGC GCTCTTC(1/4)
SbfI 1234 CCTGCAGG CCTGCA^GG
SfcI 1235 CTGCAG C^T[GA][CT]AG
SfoI 670 GGCGCC GGC^GCC
SphI 321 GCATGC GCATG^C
Sse8387I 1234 CCTGCAGG CCTGCA^GG
StyI 710 CCAAGG C^C[AT][AT]GG
Van91I 1074 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1163 CCATCGCCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 241 AAATTT [GA]^AATT[CT]
1499 AAATTC
ApoI 241 AAATTT [GA]^AATT[CT]
1499 AAATTC
AvaII 900 GGTCC G^G[AT]CC
1008 GGTCC
BalI 680 TGGCCA TGG^CCA
768 TGGCCA
BanI 670 GGCGCC G^G[CT][GA]CC
1375 GGTGCC
BanII 400 GAGCTC G[GA]GC[CT]^C
1021 GAGCTC
BglI 673 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1168 GCCAGCTTGGC
BsaJI 710 CCAAGG C^C[ACGT][ACGT]GG
1355 CCTGGG
BsgI 932 CTGCAC GTGCAG(16/14)
1439 CTGCAC
BsrDI 863 CATTGC GCAATG(2/0)
1221 CATTGC
BstYI 927 AGATCC [GA]^GATC[CT]
1126 GGATCT
EaeI 680 TGGCCA [CT]^GGCC[GA]
768 TGGCCA
HindIII 823 AAGCTT A^AGCTT
1449 AAGCTT
MaeII 302 ACGT A^CGT
1142 ACGT
MflI 927 AGATCC [GA]^GATC[CT]
1126 GGATCT
MluNI 680 TGGCCA TGG^CCA
768 TGGCCA
MscI 680 TGGCCA TGG^CCA
768 TGGCCA
NlaIV 670 GGCGCC GG[ACGT]^[ACGT]CC
1375 GGTGCC
SacI 400 GAGCTC GAGCT^C
1021 GAGCTC
SspI 218 AATATT AAT^ATT
229 AATATT
TaqI 223 TCGA T^CGA
1371 TCGA
Tsp45I 29 GTGAC ^GT[GC]AC
1409 GTGAC
TthHB8I 223 TCGA T^CGA
1371 TCGA
XhoII 927 AGATCC [GA]^GATC[CT]
1126 GGATCT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BbvI BfaI BmyI BpmI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NspI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I TspRI