(Circular) (Six-base) MAPSORT of: pFN21ASDA0020.seq Check: 4060 from: 1 to: 6253 pFN21ASDA0020 6253 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4063 278 Size: 53 83 186 936 2527 2468 Fragments arranged by size: 2527 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2670 3596 1937 Size: 733 926 4594 Fragments arranged by size: 4594 926 733 Acc65I G'GTAC_C Cuts at: 3574 3574 Size: 6253 AclI AA'CG_TT Cuts at: 1563 4380 4753 1563 Size: 2817 373 3063 Fragments arranged by size: 3063 2817 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3296 3392 4316 5700 5968 1632 Size: 1664 96 924 1384 268 1917 Fragments arranged by size: 1917 1664 1384 924 268 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6056 AflIII A'CryG_T Cuts at: 1203 5152 6129 1203 Size: 3949 977 1327 Fragments arranged by size: 3949 1327 977 AgeI A'CCGG_T Cuts at: 5084 5084 Size: 6253 AhdI GACnn_n'nnGTC Cuts at: 4982 4982 Size: 6253 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6253 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3255 3287 3359 3391 3255 Size: 32 72 32 6117 Fragments arranged by size: 6117 72 32 32 AlwNI CAG_nnn'CTG Cuts at: 3996 5568 3996 Size: 1572 4681 ApaLI G'TGCA_C Cuts at: 4310 5466 4310 Size: 1156 5097 ApoI r'AATT_y Cuts at: 1493 2264 3522 3562 3788 3940 6049 1493 Size: 771 1258 40 226 152 2109 1697 Fragments arranged by size: 2109 1697 1258 771 226 152 40 AseI AT'TA_AT Cuts at: 160 1032 4807 160 Size: 872 3775 1606 Fragments arranged by size: 3775 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 AvaI C'yCGr_G Cuts at: 1870 1943 3578 1870 Size: 73 1635 4545 Fragments arranged by size: 4545 1635 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3015 3048 3015 Size: 33 6220 BamHI G'GATC_C Cuts at: 3583 3950 3583 Size: 367 5886 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2693 3398 3574 5029 618 Size: 325 217 627 906 705 176 1455 1842 Fragments arranged by size: 1842 1455 906 705 627 325 217 176 BanII G_rGCy'C Cuts at: 1391 2427 3048 3572 1391 Size: 1036 621 524 4072 Fragments arranged by size: 4072 1036 621 524 BbeI G_GCGC'C Cuts at: 2697 2697 Size: 6253 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2771 928 Size: 971 872 4410 Fragments arranged by size: 4410 971 872 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4444 4478 1903 Size: 34 2507 34 3678 Fragments arranged by size: 3678 2507 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4145 5355 4145 Size: 1210 5043 BclI T'GATC_A Cuts at: 1548 1548 Size: 6253 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2702 3197 4864 Size: 107 122 71 631 605 1030 495 1667 Cuts at: 4864 136 Size: 1525 Fragments arranged by size: 1667 1525 1030 631 605 495 122 107 71 BglII A'GATC_T Cuts at: 6248 6248 Size: 6253 BlpI GC'TnA_GC Cuts at: 3670 3670 Size: 6253 Bme1580I G_kGCm'C Cuts at: 1790 4314 5470 1790 Size: 2524 1156 2573 Fragments arranged by size: 2573 2524 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6253 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3984 4942 442 Size: 416 53 83 399 24 2567 958 1753 Fragments arranged by size: 2567 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3048 3180 3407 4913 1239 Size: 1809 132 227 1506 2579 Fragments arranged by size: 2579 1809 1506 227 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4378 5243 5541 4378 Size: 865 298 5090 Fragments arranged by size: 5090 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4916 882 Size: 617 3417 2219 Fragments arranged by size: 3417 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2326 3550 4044 493 Size: 711 1122 1224 494 2702 Fragments arranged by size: 2702 1224 1122 711 494 BsaBI GATnn'nnATC Cuts at: 6247 6247 Size: 6253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2694 4060 4442 5077 Size: 53 83 186 936 1161 1366 382 635 Cuts at: 5077 275 Size: 1451 Fragments arranged by size: 1451 1366 1161 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4686 5084 5358 5505 5916 4686 Size: 398 274 147 411 5023 Fragments arranged by size: 5023 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3030 3060 3310 3340 1178 Size: 30 165 30 1627 30 250 30 4091 Fragments arranged by size: 4091 1627 250 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3410 1186 Size: 2224 4029 BseYI C'CCAG_C Cuts at: 5456 5952 5456 Size: 496 5757 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2940 3447 1163 Size: 573 1204 507 3969 Fragments arranged by size: 3969 1204 573 507 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3703 4464 5492 1540 Size: 275 186 1702 761 1028 2301 Fragments arranged by size: 2301 1702 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2427 3048 3545 4314 4399 5470 2427 Size: 621 497 769 85 1071 3210 Fragments arranged by size: 3210 1071 769 621 497 85 BsmI GAATG_Cn' Cuts at: 3187 3764 3857 3187 Size: 577 93 5583 Fragments arranged by size: 5583 577 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3156 3156 Size: 6253 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2427 3048 3545 3572 4314 4399 5470 Size: 399 637 621 497 27 742 85 1071 Cuts at: 5470 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 637 621 497 399 85 27 BspEI T'CCGG_A Cuts at: 5916 5916 Size: 6253 BspHI T'CATG_A Cuts at: 4142 5872 4142 Size: 1730 4523 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3090 3608 844 Size: 2246 518 3489 Fragments arranged by size: 3489 2246 518 BsrBI CCG'CTC Cuts at: 1394 1823 4140 1394 Size: 429 2317 3507 Fragments arranged by size: 3507 2317 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2885 3243 4748 4922 65 Size: 1183 1637 358 1505 174 1396 Fragments arranged by size: 1637 1505 1396 1183 358 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4897 5084 1700 Size: 110 3087 187 2869 Fragments arranged by size: 3087 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6253 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6253 BssSI C'ACGA_G Cuts at: 2142 4313 5325 2142 Size: 2171 1012 3070 Fragments arranged by size: 3070 2171 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6253 BstBI TT'CG_AA Cuts at: 2007 5057 2007 Size: 3050 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6253 BstYI r'GATC_y Cuts at: 1972 2950 3149 3583 3950 4335 4352 5804 5890 Size: 978 199 434 367 385 17 1452 86 Cuts at: 5890 5902 6248 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 978 434 385 367 346 199 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5701 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3172 487 Size: 683 56 1946 3568 Fragments arranged by size: 3568 1946 683 56 BtsI GCAGTG_nn' Cuts at: 2710 3769 4563 4583 2710 Size: 1059 794 20 4380 Fragments arranged by size: 4380 1059 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6253 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6218 DraI TTT'AAA Cuts at: 2317 3557 2317 Size: 1240 5013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5260 809 Size: 507 3944 1802 Fragments arranged by size: 3944 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2703 2791 3700 Size: 54 1425 50 275 18 873 88 909 Cuts at: 3700 4589 6218 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 909 889 873 275 88 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3700 1537 Size: 275 1888 4090 Fragments arranged by size: 4090 1888 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 4183 1353 Size: 1062 1768 3423 Fragments arranged by size: 3423 1768 1062 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4840 5214 5360 387 Size: 1064 138 3251 374 146 1280 Fragments arranged by size: 3251 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2425 3046 2425 Size: 621 5632 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3048 3180 3296 3392 3407 4316 4913 Size: 393 1416 132 116 96 15 909 597 Cuts at: 4913 5700 5968 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1416 909 787 597 393 268 132 116 96 15 EcoO109I rG'GnC_Cy Cuts at: 2923 2923 Size: 6253 EcoRI G'AATT_C Cuts at: 3562 3940 3562 Size: 378 5875 FspI TGC'GCA Cuts at: 4759 4759 Size: 6253 HaeII r_GCGC'y Cuts at: 1678 2697 5400 1678 Size: 1019 2703 2531 Fragments arranged by size: 2703 2531 1019 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2527 2559 3030 3062 4894 Size: 32 135 32 955 32 471 32 1832 Cuts at: 4894 4926 4968 5000 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1832 955 471 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3597 3843 669 Size: 988 281 1659 246 3079 Fragments arranged by size: 3079 1659 988 281 246 HindIII A'AGCT_T Cuts at: 748 2846 3472 748 Size: 2098 626 3529 Fragments arranged by size: 3529 2098 626 HpaI GTT'AAC Cuts at: 3843 3843 Size: 6253 KasI G'GCGC_C Cuts at: 2693 2693 Size: 6253 KpnI G_GTAC'C Cuts at: 3578 3578 Size: 6253 MfeI C'AATT_G Cuts at: 3852 3852 Size: 6253 MluI A'CGCG_T Cuts at: 6129 6129 Size: 6253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5367 5551 1305 Size: 4062 184 2007 Fragments arranged by size: 4062 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2705 2793 10 Size: 54 1425 343 873 88 3470 Fragments arranged by size: 3470 1425 873 343 88 54 MslI CAynn'nnrTG Cuts at: 518 1166 3219 4211 4570 4729 518 Size: 648 2053 992 359 159 2042 Fragments arranged by size: 2053 2042 992 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3669 4346 5494 5739 1606 Size: 2063 677 1148 245 2120 Fragments arranged by size: 2120 2063 1148 677 245 NarI GG'CG_CC Cuts at: 2694 2694 Size: 6253 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5701 NdeI CA'TA_TG Cuts at: 387 387 Size: 6253 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6253 NotI GC'GGCC_GC Cuts at: 3700 3700 Size: 6253 NspI r_CATG'y Cuts at: 1132 1229 2348 2413 3356 3611 5156 1132 Size: 97 1119 65 943 255 1545 2229 Fragments arranged by size: 2229 1545 1119 943 255 97 65 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6253 PciI A'CATG_T Cuts at: 5152 5152 Size: 6253 PflMI CCAn_nnn'nTGG Cuts at: 1108 3103 1108 Size: 1995 4258 PmeI GTTT'AAAC Cuts at: 3557 3557 Size: 6253 PmlI CAC'GTG Cuts at: 2326 2326 Size: 6253 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4322 4354 5835 5867 4322 Size: 32 1481 32 4708 Fragments arranged by size: 4708 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2923 2923 Size: 6253 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6253 PsiI TTA'TAA Cuts at: 3823 3823 Size: 6253 PstI C_TGCA'G Cuts at: 830 3262 3605 830 Size: 2432 343 3478 Fragments arranged by size: 3478 2432 343 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6253 SacI G_AGCT'C Cuts at: 2427 3048 2427 Size: 621 5632 SalI G'TCGA_C Cuts at: 1936 3595 1936 Size: 1659 4594 SapI GCTCTTCn'nnn_ Cuts at: 2415 2415 Size: 6253 SbfI CC_TGCA'GG Cuts at: 3262 3605 3262 Size: 343 5910 ScaI AGT'ACT Cuts at: 4501 6213 4501 Size: 1712 4541 SfcI C'TryA_G Cuts at: 826 1045 3258 3601 4736 5417 5608 826 Size: 219 2213 343 1135 681 191 1471 Fragments arranged by size: 2213 1471 1135 681 343 219 191 SfoI GGC'GCC Cuts at: 2695 2695 Size: 6253 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6253 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6253 SmaI CCC'GGG Cuts at: 1872 3580 1872 Size: 1708 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4357 5258 5520 820 Size: 197 926 2414 901 262 1553 Fragments arranged by size: 2414 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3550 4044 493 Size: 3057 494 2702 Fragments arranged by size: 3057 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6253 SphI G_CATG'C Cuts at: 1132 2348 3611 1132 Size: 1216 1263 3774 Fragments arranged by size: 3774 1263 1216 SspI AAT'ATT Cuts at: 5 52 2243 2254 4177 5 Size: 47 2191 11 1923 2081 Fragments arranged by size: 2191 2081 1923 47 11 StuI AGG'CCT Cuts at: 1517 1517 Size: 6253 StyI C'CwwG_G Cuts at: 513 1065 2733 3692 513 Size: 552 1668 959 3074 Fragments arranged by size: 3074 1668 959 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4280 4297 4450 4635 5080 325 Size: 1255 2700 17 153 185 445 1498 Fragments arranged by size: 2700 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2931 4499 6211 Size: 274 80 33 51 1443 954 1568 1712 Cuts at: 6211 96 Size: 138 Fragments arranged by size: 1712 1568 1443 954 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6213 6245 6213 Size: 32 6221 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6253 XbaI T'CTAG_A Cuts at: 3589 3589 Size: 6253 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3193 3193 Size: 6253 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6253 XmaI C'CCGG_G Cuts at: 1870 3578 1870 Size: 1708 4545 XmnI GAAnn'nnTTC Cuts at: 4382 4382 Size: 6253 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4061 276 Size: 53 83 186 936 2527 2468 Fragments arranged by size: 2527 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI